动物营养学报 >

2020 , Vol. 32 >Issue 4: 1946 - 1956

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.04.055

研究简报 SHORT COMMUNICATION

玉米赤霉烯酮与脱氧雪腐镰刀菌烯醇联合染毒对雄性小鼠生殖毒性的影响及茶多酚的缓解作用

  • 曹利 ,
  • 赵杰 ,
  • 陈铃霞 ,
  • 谢昕 ,
  • 冉梦 ,
  • 申茂玉 ,
  • 朱雷 ,
  • 冯士彬 ,
  • 李玉 ,
  • 吴金节 ,
  • 王希春
展开
  • 安徽农业大学动物科技学院, 合肥 230036
曹利(1995-),女,安徽六安人,硕士研究生,研究方向为畜禽中毒病。E-mail:17352967806@163.com

收稿日期: 2019-09-30

  网络出版日期: 2020-04-16

基金资助

安徽省生猪产业技术体系资助项目（AHCYJSTX-05-07）

收起

Effects of Combined Exposure of Zearalenone and Deoxynivalenol on Reproductive Toxicity of Male Mice and Alleviation of Tea Polyphenols

  • CAO Li ,
  • ZHAO Jie ,
  • CHEN Lingxia ,
  • XIE Xin ,
  • RAN Meng ,
  • SHEN Maoyu ,
  • ZHU Lei ,
  • FENG Shibin ,
  • LI Yu ,
  • WU Jinjie ,
  • WANG Xichun
Expand
  • College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2019-09-30

  Online published: 2020-04-16

Supported by

 

Fold

摘要

本试验旨在研究茶多酚对玉米赤霉烯酮（ZEN）与脱氧雪腐镰刀菌烯醇（DON）联合染毒小鼠生殖毒性损伤的缓解作用。选取8周龄雄性昆明小鼠75只[体重（40±5）g]，随机分成5组，每组分别灌服生理盐水（对照组）、200 mg/kg茶多酚（Ⅰ组）、1 mg/kg ZEN+0.5 mg/kg DON（Ⅱ组）、100 mg/kg茶多酚+1 mg/kg ZEN+0.5 mg/kg DON（Ⅲ组）和200 mg/kg茶多酚+1 mg/kg ZEN+0.5 mg/kg DON（Ⅳ组），每组15只。试验期28 d。在试验第1、14、28天，从每组随机抽取5只小鼠称重采样，检测小鼠精子质量、睾丸脏器系数、睾丸组织标志酶活性、血清性激素含量及睾丸组织氧化与抗氧化指标，并制作睾丸组织切片。结果表明：1）与对照组相比，Ⅱ组小鼠睾丸脏器系数、精子活率及数量、血清性激素含量显著降低（P<0.05），睾丸组织碱性磷酸酶（AKP）、乳酸脱氢酶（LDH）、γ-谷氨酰转移酶（γ-GGT）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）及谷胱甘肽过氧化物酶（GSH-Px）活性显著降低（P<0.05）；精子畸形率、血清丙二醛（MAD）含量显著上升（P<0.05）。添加茶多酚后，小鼠的睾丸脏器系数、精子活率及数量、血清性激素含量、睾丸组织AKP、LDH、γ-GGT、CAT、SOD及GSH-Px活性显著上升（P<0.05），精子畸形率、血清MAD含量显著降低（P<0.05）。随着时间的推移，与第1天相比，Ⅰ组小鼠睾丸组织GSH-Px活性显著升高（P<0.05），Ⅱ组小鼠睾丸脏器系数、精子质量、睾丸组织标志酶活性、血清性激素含量、睾丸组织氧化与抗氧化指标均发生显著变化（P<0.05），Ⅲ组小鼠睾丸组织CAT及GSH-Px活性显著降低（P<0.05），Ⅳ组小鼠睾丸组织CAT活性显著降低（P<0.05）。2）睾丸组织病理切片显示染毒组小鼠睾丸生精小管基膜不完整，细胞排列紊乱，数量减少，体积缩小，细胞之间空泡化，管腔内精子数量减少，经茶多酚保护后得到改善。综上，茶多酚能有效改善ZEN与DON联合染毒小鼠精子质量与睾丸组织内标志酶活性，提高血清性激素含量及睾丸组织抗氧化功能，显著降低生殖毒性。

关键词： 茶多酚; 玉米赤霉烯酮; 脱氧雪腐镰刀菌烯醇; 小鼠; 生殖毒性

本文引用格式

曹利 , 赵杰 , 陈铃霞 , 谢昕 , 冉梦 , 申茂玉 , 朱雷 , 冯士彬 , 李玉 , 吴金节 , 王希春 . 玉米赤霉烯酮与脱氧雪腐镰刀菌烯醇联合染毒对雄性小鼠生殖毒性的影响及茶多酚的缓解作用[J]. 动物营养学报, 2020 , 32(4) : 1946 -1956 . DOI: 10.3969/j.issn.1006-267x.2020.04.055

Abstract

The present experiment was conducted to evaluate the alleviation of tea polyphenols on reproductive toxicity injury of mice induced by zearalenone (ZEN) and deoxynivalenol (DON). Seventy-five 8-week-old Kunming (male) mice were randomly divided into 5 groups, and each group was administered saline (control group), 200 mg/kg tea polyphenols (group Ⅰ), 1 mg/kg ZEN+0.5 mg/kg DON (group Ⅱ), 100 mg/kg tea polyphenols+1 mg/kg ZEN+0.5 mg/kg DON (group Ⅲ) and 200 mg/kg tea polyphenols+1 mg/kg ZEN+0.5 mg/kg DON (group Ⅳ), respectively, 15 mice in each group. The experiment lasted for 28 d. On the days 1, 14 and 28, five mice in each group were randomly selected and weighed, the sperm quality, testicular organ coefficient, testicular tissue marker enzymes activities, serum sex hormone contents, testicular tissue oxidation and antioxidant indexes of mice were detected, and testicular tissue sections were prepared. The results showed as follows:1) compared with the control group, testicular organ coefficient, sperm survival rate and quantity, serum sex hormone contents of mice in group Ⅱ were decreased significantly (P<0.05), the activities of alkaline phosphatase (AKP), lactate dehydrogenase (LDH), γ-glutamyltransferase (γ-GGT), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in testicular tissue were decreased significantly (P<0.05) and the sperm abnormality rate and the content of malondialdehyde (MAD) in serum increased significantly (P<0.05). After adding tea polyphenols, testicular organ coefficient, sperm survival rate and quantity, serum sex hormone contents, the activities of AKP, LDH, γ-GGT, CAT, SOD and GSH-Px in testicular tissue were increased significantly (P<0.05), the sperm abnormality rate and the content of MDA in serum were decreased significantly (P<0.05). Over time, compared with day 1, the activity of GSH-Px in testicular tissue in group Ⅰ was significantly increased (P<0.05), the testicular organ coefficient, sperm quality, testicular tissue marker enzymes activities, serum sex hormone contents, testicular tissue oxidation and antioxidant indexes in group Ⅱ were significantly changed (P<0.05), the activities of CAT and GSH-Px in testicular tissue in group Ⅲ were significantly decreased (P<0.05), the activity of CAT in testicular tissue in group Ⅳ was significantly decreased (P<0.05). 2) Histopathological sections of testis showed that the basement membrane of spermatogenic tubules was incomplete, cells arranged disorderly, the number and volume of spermatogenic tubules decreased, vacuolation between cells and sperm quantity decreased in the testicular lumen of mice in the exposed group, and improvement after protection by tea polyphenols. These results suggest that tea polyphenols can effectively improve the quality of sperm and the activities of marker enzymes in testicular tissues, the contnets of serum sex hormones and the antioxidant function of testicular tissues, decrease the reproductive toxicity of mice induced by ZEN and DON.

Key words： tea polyphenols; zearalenone; deoxynivalenol; mice; reproductive toxicity

参考文献

[1] JUAN C,RAIOLA A,MAÑES J,et al.Presence of mycotoxin in commercial infant formulas and baby foods from Italian market[J].Food Control,2014,39:227-236.
[2] SMITH J E,SOLOMONS G,LEWIS C,et al.Role of mycotoxins in human and animal nutrition and health[J].Neurogastroenterology & Motility,2010,3(4):187-192.
[3] ABID-ESSEFI S,OUANES Z,HASSEN W,et al.Cytotoxicity,inhibition of DNA and protein syntheses and oxidative damage in cultured cells exposed to zearalenone[J].Toxicology in Vitro,2004,18(4):467-474.  
[4] PETROV V,QURESHI M K,HILLE J,et al.Occurrence,biochemistry and biological effects of host-selective plant mycotoxins[J].Food and Chemical Toxicology,2018,112:251-264.
[5] 闫昭明,陈清华,陈凤鸣.玉米赤霉烯酮毒性研究[J].动物营养学报,2018,30(9):3453-3458.
[6] SPRANDO R L,COLLINS T F X,BLACK T N,et al.Characterization of the effect of deoxynivalenol on selected male reproductive endpoints[J].Food and Chemical Toxicology,2005,43(4):623-635.  
[7] AHMED K K M,BARAL M.Tea polyphenols as antioxidants[J].Free Radicals and Antioxidants,2011,1(2):2-3.  
[8] 石惠宇,闫素梅.维生素A对动物氧化应激的减缓作用机制[J].动物营养学报,2019,31(6):2458-2464.
[9] LAMBERT J D,YANG C S.Mechanisms of cancer prevention by tea constituents[J].The Journal of Nutrition,2003,133(10):3262S-3267S.
[10] FRAGA C G,CROFT K D,KENNEDY D O,et al.The effects of polyphenols and other bioactives on human health[J].Food & Function,2019,10(2):514-528.  
[11] SENTKOWSKA A,PYRZYŃ SKA K.Investigation of antioxidant activity of selenium compounds and their mixtures with tea polyphenols[J].Molecular Biology Reports,2019,46(3):3019-3024.  
[12] 王慧敏,朱军莉,陆海霞,等.茶多酚抑制腐败希瓦氏菌机理研究[J].茶叶科学,2014,34(2):149-155.
[13] 李振,陈现伟.茶多酚的免疫调节作用及应用[J].中国兽药杂志,2004,38(4):33-35.
[14] 张劲,彭天英,张令君,等.茶多酚提取技术及其功能化研究进展[J].广东化工,2019,46(4):86-87.
[15] 元辉雄,庞艳芳,王俊利,等.铝对大鼠精子质量及精子线粒体的影响[J].中华男科学杂志,2019,25(7):579-585.
[16] 王文祥,张文昌,廖惠珍.茶多酚对镉致大鼠精子损伤的保护作用[J].福建医科大学学报,2011,45(5):319-322,326.
[17] 黄菊,张光佑,沈伶,等.茶多酚对大鼠卵巢卵泡发育的影响[J].汕头大学医学院学报,2015,28(2):94-96.
[18] 逄健,孙中义.小剂量玉米赤霉烯酮损害雄性小鼠生殖能力的研究[J].第三军医大学学报,2017,39(12):1225-1230.
[19] 赵丽华,张爱君.砷中毒对大鼠睾丸组织及精子形态的影响[J].中国地方病防治杂志,2018,33(4):370,446.
[20] IBRAHIM A,NATARAJAN S,GHAFOORUNISSA.Dietary trans-fatty acids alter adipocyte plasma membrane fatty acid composition and insulin sensitivity in rats[J].Metabolism,2005,54(2):240-246.  
[21] MOZAFFARIAN D,PISCHON T,HANKINSON S E,et al.Dietary intake of trans fatty acids and systemic inflammation in women[J].The American Journal of Clinical Nutrition,2004,79(4):606-612.  
[22] HASCHEK W M,HALIBURTON J C.Fusarium moniliforme and zearalenone toxicoses in domestic animals:a review[M]//RICHARD J L,THURSTON J R.Diagnosis of mycotoxicoses.Dordrecht:Springer,1986:156.
[23] 刘彩艳.猪玉米赤霉烯酮中毒的临床表现及其治疗[J].现代畜牧科技,2016(9):114.
[24] 凌阿茹.脱氧雪腐镰刀菌烯醇诱导睾丸组织细胞凋亡及精子损伤的作用机制研究[C]//中国毒理学会第七次全国会员代表大会暨中国毒理学会第六次中青年学者科技论坛论文摘要.北京:中国毒理学会,2018:2.
[25] 樊友平.精液微量元素锌、硒对精子质量的影响[J].生殖与避孕,2005(1):63.
[26] 王卫杰,李惠,王乐,等.饲料添加剂的蓄积对幼犬睾丸功能相关酶活性的影响[J].饲料博览,2016(7):1-3.
[27] 张波,徐光翠,刘峰,等.氧乐果对小鼠睾丸特征性酶的影响及茶多酚的拮抗作用[J].环境与健康杂志,2005,22(5):340-342.
[28] GENG X,SHAO H,ZHANG Z H,et al.Malathion-induced testicular toxicity is associated with spermatogenic apoptosis and alterations in testicular enzymes and hormone levels in male Wistar rats[J].Environmental Toxicology and Pharmacology,2015,39(2):659-667.  
[29] 郭海,才秀莲,王国秀.锰诱导大鼠生精细胞凋亡过程中半胱氨酸酶-3 mRNA与增殖细胞核抗原表达变化[J].解剖学杂志,2015,38(1):15-17,103.
[30] 李晓凤,杨旸,周义军.妊娠期氰戊菊酯暴露对雄性仔鼠睾丸标志酶的影响[J].上海交通大学学报(医学版),2011,31(8):1117-1120.
[31] 丁皎,孙应彪,常旭红,等.硫酸镍对体外小鼠精原细胞所致氧化应激水平的研究[J].毒理学杂志,2010,24(1):34-37.
[32] 王希春,曹利,刘秦,等.脱氧雪腐镰刀菌烯醇和黄曲霉毒素B1单一及联合染毒对小鼠脑组织病理变化、抗氧化性能和紧密连接蛋白表达量的影响[J].浙江农业学报,2019,31(6):886-892.
[33] 杨小军,贺喜,何丽霞,等.日粮添加多不饱和脂肪酸对肉仔鸡抗氧化指标的影响[J].动物营养学报,2008,20(3):299-304.
[34] UTOMO A,JIANG X Z,FURUTA S,et al.Identification of a novel putative non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) essential for alleviating oxidative stress generated from polyunsaturated fatty acids in breast cancer cells[J].Journal of Biological Chemistry,2004,279(42):43522-43529.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2