动物营养学报 >

2020 , Vol. 32 >Issue 5: 2126 - 2137

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.05.021

禽营养与饲料 POULTRY NUTRITION AND FEED

外源褪黑素对产蛋后期蛋鸡生产性能、血清指标及体内褪黑素含量的影响

  • 郝二英 ,
  • 黄晨轩 ,
  • 孙浩政 ,
  • 王德贺 ,
  • 周荣艳 ,
  • 黄仁录 ,
  • 陈辉
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  • 河北农业大学动物科技学院, 保定 071000
郝二英(1989-),女,河北石家庄人,博士研究生,从事单胃动物营养的研究。E-mail:277179119@qq.com

收稿日期: 2019-10-29

  网络出版日期: 2020-05-15

基金资助

国家蛋鸡产业技术体系（CARS-40-K20）

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Effects of Exogenous Melatonin on Performance, Serum Indexes and Melatonin Content of Laying Hens during Later Laying Period

  • HAO Erying ,
  • HUANG Chenxuan ,
  • SUN Haozheng ,
  • WANG Dehe ,
  • ZHOU Rongyan ,
  • HUANG Renlu ,
  • CHEN Hui
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  • College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China

Received date: 2019-10-29

  Online published: 2020-05-15

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摘要

为了研究不同浓度的外源褪黑素对产蛋后期蛋鸡生产性能、体内褪黑素含量及血清指标的影响，本试验随机选取健康、体重相近的70周龄大午金凤蛋鸡60只，随机分为3组，每组4个重复，每个重复5只。对照组腹腔注射等量生理盐水，试验Ⅰ组腹腔注射褪黑素20 mg/（kg·d），试验Ⅱ组腹腔注射褪黑素50 mg/（kg·d）。光照条件为16L：8D，预试期7 d，正试期28 d。结果显示：1）与对照组相比，褪黑素处理极显著提高了第15~21天和第22~28天蛋鸡的产蛋率（P<0.01），显著提高了大白卵泡的数量（P<0.05），但并没有对蛋品质造成显著的影响（P>0.05）。2）与对照组相比，在第21和28天时，褪黑素处理显著或极显著提高了血清和蛋黄中的褪黑素含量（P<0.05或P<0.01）；在第28天时，50 mg/（kg·d）褪黑素极显著提高了卵巢中的褪黑素含量（P<0.01）。3）与对照组相比，在第21和28天时，50 mg/（kg·d）褪黑素极显著提高了血清和蛋黄中超氧化物歧化酶（SOD）活性及总抗氧化能力（T-AOC）（P<0.01），极显著降低了氧自由基（ROS）含量（P<0.01）；在第28天时，50 mg/（kg·d）褪黑素极显著提高了卵巢中SOD活性及T-AOC（P<0.01），极显著降低了ROS含量（P<0.01）。4）与对照组相比，在第21和28天时，50 mg/（kg·d）褪黑素极显著提高了血清免疫球蛋白（Ig）A、IgG、IgM含量（P<0.01）。5）与对照组相比，在第21和28天时，褪黑素处理极显著提高了血清促卵泡激素（FSH）和促黄体素（LH）含量（P<0.01）。综上，外源褪黑素显著提高了产蛋后期蛋鸡的产蛋率，提高了机体的抗氧化性能、免疫性能及生殖激素水平，为产蛋后期外源补充褪黑素提高产蛋性能提供理论依据。

关键词： 褪黑素; 产蛋后期; 产蛋率; 抗氧化指标; 卵泡数量

本文引用格式

郝二英 , 黄晨轩 , 孙浩政 , 王德贺 , 周荣艳 , 黄仁录 , 陈辉 . 外源褪黑素对产蛋后期蛋鸡生产性能、血清指标及体内褪黑素含量的影响[J]. 动物营养学报, 2020 , 32(5) : 2126 -2137 . DOI: 10.3969/j.issn.1006-267x.2020.05.021

Abstract

To investigate the effects of different concentrations of exogenous melatonin on performance, melatonin content and serum indexes of laying hens during later laying period, 60 healthy Dawu Jinfeng laying hens with similar healthy body weight were randomly divided into 3 groups, each group had 4 repetitions, and each had 5 repetitions. The control group was intraperitoneally injected normal saline. The test group Ⅰ was injected intraperitoneally with 20 mg/(kg·d) melatonin, and the test group Ⅱ was intraperitoneally injected with 50 mg/(kg·d) melatonin. The illumination condition was 16L:8D, the test period included a 7-d pre-test period and a 28-d trial period. The results showed as follows:1) compared with the control group, the melatonin treatment significantly increased the laying rate at d 15 to 21 and d 22 to 28 (P<0.01), and significantly increased the number of large white follicles (P<0.05), but had no significant effect on egg quality (P>0.05). 2) Compared with the control group, the melatonin treatment significantly increased the melatonin contents in the serum and yolk at d 21 and 28 (P<0.05 or P<0.01); at d 28, the 50 mg/(kg·d) melatonin treatment significantly increased the melatonin contnet in the ovaries (P<0.01). 3) Compared with the control group, at d 21 and 28, 50 mg/(kg·d) melatonin significantly increased the superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) (P<0.01), and significantly reduced the content of oxygen free radicals (ROS) (P<0.01); at d 28, 50 mg/(kg·d) melatonin significantly increased the activity of SOD and T-AOC (P<0.01), and significantly reduced the content of ROS in the ovary (P<0.01). 4) Compared with the control group, at d 21 and 28, 50 mg/(kg·d) melatonin significantly increased the serum immunoglobulin (Ig) A, IgG and IgM contents (P<0.01). 5) Compared with the control group, melatonin treatment significantly increased the follicle-stimulating hormone (FSH) and luteotropin (LH) contents in serum at d 21 and 28 (P<0.01). Exogenous melatonin treatment significantly improves the laying rate of laying hens in the later laying period, improves the body's antioxidant performance, immune performance, and reproductive hormone levels. It provided a theoretical basis for exogenous melatonin supplementation to improve egg production during the end of laying.

Key words： melatonin; later laying period; laying rate; antioxidant index; number of follicles

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