动物营养学报 >

2020 , Vol. 32 >Issue 5: 2164 - 2175

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.05.025

禽营养与饲料 POULTRY NUTRITION AND FEED

注射胰岛素对不同品种鸡血糖浓度和采食情况的影响

  • 王焕杰 ,
  • 杜鹏飞 ,
  • 张吉昌 ,
  • 朱瑶 ,
  • 姬杰菲 ,
  • 陈文 ,
  • 黄艳群
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  • 1. 河南农业大学饲料营养河南省工程实验室, 郑州 450002;
    2. 南阳市宛城区动物卫生监督所, 南阳 473000
王焕杰(1993-),女,河南周口人,硕士研究生,动物营养与饲料科学专业。E-mail:1932515966@qq.com

收稿日期: 2019-11-21

  网络出版日期: 2020-05-15

基金资助

智汇郑州1125聚才计划——创新领军人才项目

收起

Effects of Insulin Injection on Blood Glucose Concentration and Feeding Status of Different Breed Chickens

  • WANG Huanjie ,
  • DU Pengfei ,
  • ZHANG Jichang ,
  • ZHU Yao ,
  • JI Jiefei ,
  • CHEN Wen ,
  • HUANG Yanqun
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  • 1. Feed Nutrition Engineering Laboratory of Henan Province, Henan Agricultural University, Zhengzhou 450002, China;
    2. Nanyang Wancheng District Animal Health Supervision, Nanyang 473000, China

Received date: 2019-11-21

  Online published: 2020-05-15

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摘要

本试验旨在探究注射胰岛素对不同品种鸡血糖浓度和采食情况的影响。试验1选用21日龄海兰蛋鸡（简称蛋鸡）、丝毛乌骨鸡（简称乌鸡）和爱拔益加（AA）肉鸡（简称肉鸡）雄性雏鸡各120只，随机各分为4组：胰岛素禁食组（n=60，其中12只鸡用来测定血糖浓度的动态变化，其余48只鸡在注射胰岛素后0、15、120和240 min时，每个时间点各屠宰12只鸡，收集颈静脉血液10 mL，用来测定血清胰岛素浓度）、磷酸盐缓冲液（PBS）禁食组（n=12）、胰岛素喂食组（n=24）和PBS喂食组（n=24）。试验前禁食16 h，注射剂量为5 IU/kg的胰岛素或相同剂量的PBS，禁食各组在注射后继续保持禁食，并测定血糖浓度的动态变化；喂食各组在注射后立即恢复喂食，1/2鸡只用于测定血糖浓度的动态变化，1/2鸡只用于统计采食情况。试验2选用44日龄蛋鸡、乌鸡和肉鸡各48只，随机各分为2组：胰岛素组（n=24）和PBS组（n=24），试验前禁食16 h，注射剂量为2.3 IU/kg的胰岛素或相同剂量的PBS，注射120 min后恢复喂食，各组鸡1/2鸡只用于测定血糖浓度的动态变化，1/2鸡只用于统计采食情况。结果表明：1）禁食状态下，注射胰岛素可显著降低21日龄各品种鸡的血糖浓度（P<0.05），且注射后15 min时各品种鸡的血糖浓度均已显著低于基础（0 min）血糖浓度（P<0.05），且持续下降直到120 min时；肉鸡的血糖浓度与蛋鸡和乌鸡相比下降幅度更大，且120 min后血糖浓度恢复能力显著低于蛋鸡和乌鸡（P<0.05）；注射胰岛素后肉鸡不同时间点的血清胰岛素浓度均高于蛋鸡和乌鸡。2）与基础血糖浓度相比，21日龄时注射胰岛素后立即恢复喂食显著降低蛋鸡60和120 min时的血糖浓度（P<0.05），显著提高肉鸡30和60 min时的血糖浓度（P<0.05）。3）注射胰岛素后立即恢复喂食对21日龄各品种鸡240 min内的累积采食量无显著影响（P>0.05），显著降低蛋鸡和乌鸡注射后120~240 min时的采食量（P<0.05），显著提高肉鸡注射后30~60 min时的采食量（P<0.05）。4）注射胰岛素后120 min恢复喂食显著降低44日龄各品种鸡的采食量（P<0.05）。综上所述，各品种鸡对胰岛素的敏感性和血糖浓度响应变化存在差异，乌鸡和蛋鸡的血糖调控能力要优于肉鸡；注射胰岛素对鸡的采食量有显著影响，且对采食的影响存在品种差异。

关键词： 蛋鸡; 乌鸡; 肉鸡; 胰岛素; 血糖浓度; 采食

本文引用格式

王焕杰 , 杜鹏飞 , 张吉昌 , 朱瑶 , 姬杰菲 , 陈文 , 黄艳群 . 注射胰岛素对不同品种鸡血糖浓度和采食情况的影响[J]. 动物营养学报, 2020 , 32(5) : 2164 -2175 . DOI: 10.3969/j.issn.1006-267x.2020.05.025

Abstract

This study was conducted to explore the effects of insulin injection on blood glucose concentration and feeding status of different breed chickens. Experiment 1:one hundred and twenty male Hy-line laying hens (abbreviated as laying hens), silky black bone chickens (abbreviated as silky chickens) and Arbor Acres (AA) broiler chickens (abbreviated as broiler chickens) at 21 days of age were randomly divided into 4 groups, respectively, and the 4 groups were insulin fasting group (n=60, of which 12 chickens were used to measure dynamic changes of blood glucose concentration, and rest 48 chickens were slaughtered at 0, 15, 120 and 240 min after insulin injection and 12 chickens per time point, and 10 mL jugular blood were collected to determine serum insulin concentration), phosphate buffered saline (PBS) fasting group (n=12), insulin feeding group (n=24) and PBS feeding group (n=24). All chickens fasted for 16 h before test, injection dose was 5 IU/kg insulin or the same dosage of PBS, chickens in fasting groups were continued to fasted after injection and used to determine dynamic changes of blood glucose concentration, and chickens in feeding groups were feeding immediately after injection, one half were used to measure dynamic changes of blood glucose concentration and another half were used to statistics feeding status. Experiment 2:forty eight laying hens, silky chickens and broiler chickens at 44 days of age were randomly divided into 2 groups, respectively, insulin group (n=24) and PBS group (n=24). All chickens fasted for 16 h before test, injection dose was 2.3 IU/kg insulin or the same dosage of PBS, and feeding after 120 min of injection, one half in each group were used to measure dynamic changes of blood glucose concentration, and another half were used to statistics feeding statues. The results showed as follows:1) insulin injection significantly decreased blood glucose concentration of all breed chickens at 21 days of age in fasting state (P<0.05). Blood glucose concentration of all breed chickens was significantly lower than basic blood glucose concentration after 15 min of injection (P<0.05), and continued to drop until 120 min. Blood glucose concentration of broilers was decreased more sharply compared with laying hens and silky chickens, and recovery of blood glucose concentration was significantly lower than that of laying hens and silky chickens after 120 min (P<0.05). Serum insulin concentration of broilers at different time points was higher than that of laying hens and silky chicken after insulin injection. 2) Compared with basic blood glucose concentration, feeding immediately after insulin injection significantly decreased blood glucose concentration of laying hens at 21 days of age at 60 and 120 min (P<0.05), blood glucose concentration of broilers after insulin injection 30 and 60 min was significantly increased (P<0.05). 3) Feed immediately after insulin injection had no significant effect on cumulative feed intake of all breed chickens at 21 days of age in 240 min (P>0.05), and significantly decreased feed intake of laying hens and silky chickens after insulin injection from 120 to 240 min (P<0.05), significantly increased feed intake of broilers after insulin injection from 30 to 60 min (P<0.05). 4) Feeding after insulin injection 120 min significantly decreased food intake of all breed chickens at 44 days of age (P<0.05). In summary, there are differences in insulin sensitivity and blood glucose concentration response changes among different breed chickens, blood glucose regulation ability of silky chickens and laying hens are better than those of broilers. Insulin injection has significant effects on feed intake of chickens, and there are difference in influence on feed intake.

Key words： laying hens; silky chickens; broiler chickens; insulin; blood glucose concentration; feed intake

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