动物营养学报 >

2020 , Vol. 32 >Issue 5: 2209 - 2217

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.05.030

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

金银花提取物对热应激奶牛生产性能、血清生化指标和免疫功能的影响

  • 马峰涛 ,
  • 单强 ,
  • 金宇航 ,
  • 李洪洋 ,
  • 高铎 ,
  • 孙鹏
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  • 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193
马峰涛(1992-),男,河北石家庄人,硕士研究生,从事动物营养与饲料科学研究。E-mail:fengtaoma@qq.com

收稿日期: 2019-11-24

  网络出版日期: 2020-05-15

基金资助

国家重点研发计划（2016YFD0500507）；中国农业科学院科技创新工程（ASTIP-IAS07）

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Effects of Lonicera japonica Extract on Performance, Serum Biochemical Indexes and Immune Function of Heat-Stressed Dairy Cows

  • MA Fengtao ,
  • SHAN Qiang ,
  • JIN Yuhang ,
  • LI Hongyang ,
  • GAO Duo ,
  • SUN Peng
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  • State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2019-11-24

  Online published: 2020-05-15

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摘要

本试验旨在研究饲粮添加金银花提取物（LJE）对热应激奶牛生产性能、血清生化指标和免疫功能的影响，以探究金银花提取物有效缓解奶牛热应激的适宜添加量。试验选取20头产奶量、胎次、泌乳日龄相近的健康中国荷斯坦奶牛，随机分为4组，每组5头。对照组（CON组）饲喂基础饲粮，试验组分别在基础饲粮中添加14（LJE-14组）、28（LJE-28组）、56 g/d（LJE-56组）的金银花提取物。预试期2周，正试期8周。结果表明：1）饲粮添加金银花提取物可显著降低热应激奶牛直肠温度（P<0.05），而对呼吸频率无显著影响（P>0.05）。2）饲粮添加金银花提取物对热应激奶牛干物质采食量、产奶量、乳蛋白率、乳脂率、乳糖率、非脂乳固形物含量和总固形物含量无显著影响（P>0.05）。3）饲粮添加金银花提取物可显著降低热应激奶牛血清肌酐含量（P<0.05），对热应激奶牛血清尿酸、尿素、总蛋白、白蛋白和葡萄糖含量无显著影响（P>0.05）。随着金银花提取物添加量的增加，血清葡萄糖含量呈二次升高趋势（P=0.072）。4）LJE-28组热应激奶牛血清免疫球蛋白G和白细胞介素-4含量显著高于CON组（P<0.05）。饲粮添加金银花提取物对热应激奶牛血清免疫球蛋白A、免疫球蛋白M、白细胞介素-1β和白细胞介素-2含量无显著影响（P>0.05），有降低热应激奶牛血清热休克蛋白-72含量的趋势（P=0.084）。综上所述，饲粮添加金银花提取物对热应激奶牛生产性能无显著影响，可提高热应激奶牛免疫应答，有助于缓解奶牛热应激。本试验条件下，有效缓解奶牛热应激的金银花提取物适宜添加量为28 g/d。

关键词： 金银花提取物; 热应激; 奶牛; 生产性能; 血液清生化指标; 免疫功能

本文引用格式

马峰涛 , 单强 , 金宇航 , 李洪洋 , 高铎 , 孙鹏 . 金银花提取物对热应激奶牛生产性能、血清生化指标和免疫功能的影响[J]. 动物营养学报, 2020 , 32(5) : 2209 -2217 . DOI: 10.3969/j.issn.1006-267x.2020.05.030

Abstract

The objective of this study was to investigate the effects of Lonicera japonica extract (LJE) on performance, serum biochemical indexes and immune function of heat-stressed dairy cows, and to explore the optimum amount of LJE to effectively alleviate heat stress of dairy cows. Twenty healthy Chinese Holstein cows with similar milk yield, parity and lactation days were randomly assigned to 4 groups with 5 cows per group. Cows in the control group (CON group) were fed a basal diet, while other in experimental groups were fed basal diets supplemented with 14 (LJE-14 group), 28 (LJE-28 group) and 56 g/d (LJE-56 group) LJE, respectively. The pre-experimental period lasted for 2 weeks, and the experimental period lasted for 8 weeks. The results showed as follows:1) dietary supplementation of LJE could significantly decreased the rectal temperature of heat-stressed dairy cows (P<0.05), but had no significant effect on respiratory rate (P>0.05). 2) Dietary supplementation of LJE had no significant effects on dry matter intake, milk yield, milk fat rate, milk protein rate, lactose rate, non-fat solid content and total solid content of heat-stressed dairy cows (P>0.05). 3) Dietary supplementation of LJE could significantly decreased the serum creatinine content of heat-stressed dairy cows (P<0.05), but had no significant effect on the contents of uric acid, urea, total protein, albumin and glucose in serum (P>0.05). With the supplemental amount of LJE increased, the serum glucose content showed a quadratically increasing trend (P=0.072). 4) The contents of immunoglobulin G and interleukin-4 in serum of heat-stressed dairy cows of LJE-28 group were significantly higher than those of the CON group (P<0.05). Dietary supplementation of LJE had no significant effects on the contents of immunoglobulin A, immunoglobulin M, interleukin-1β and interleukin-2 in serum of heat-stressed dairy cows (P>0.05), and showed a trend to reduce the serum heat shock protein-72 content of heat-stressed dairy cows (P=0.084). In summary, dietary supplementation of LJE have no significant effect on the performance of heat-stressed dairy cows, can improve the immune response of heat-stressed dairy cows, and help to alleviate the heat stress of dairy cows. Under the condition of this experiment, the optimal amount for effectively alleviate heat stress of dairy cows is 28 g/d.

Key words： Lonicera japonica extract; heat stress; dairy cows; performance; serum biochemical indexes; immune function

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