动物营养学报 >

2020 , Vol. 32 >Issue 5: 2241 - 2250

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.05.033

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

葡萄籽原花青素对公羔羊生长性能、精液品质及睾丸和附睾抗氧化指标的影响

  • 牟春堂 ,
  • 杨文军 ,
  • 王鹏举 ,
  • 任国栋 ,
  • 剧浩 ,
  • 任有蛇 ,
  • 郝小燕 ,
  • 张建新
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  • 山西农业大学动物科技学院, 太谷 030801
牟春堂(1989-),男,内蒙古呼伦贝尔人,博士,从事反刍动物营养与饲料科学研究。E-mail:sxndmct@sina.com

收稿日期: 2019-11-19

  网络出版日期: 2020-05-15

基金资助

国家重点研发项目（2018YFD0502104）；国家肉羊产业技术体系项目（CARS-38）；山西省基础研究计划项目（201801D221291）；“1331工程”畜牧学重点学科建设计划项目（J201911301）

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Effects of Grape Seed Procyanidins on Growth Performance, Semen Quality, Antioxidant Indexes in Testis and Epididymis of Ram

  • MU Chuntang ,
  • YANG Wenjun ,
  • WANG Pengju ,
  • REN Guodong ,
  • JU Hao ,
  • REN Youshe ,
  • HAO Xiaoyan ,
  • ZHANG Jianxin
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  • College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China

Received date: 2019-11-19

  Online published: 2020-05-15

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摘要

本试验旨在研究饲喂葡萄籽原花青素（GSPs）对公羔羊生长性能、精液品质及睾丸和附睾抗氧化指标的影响。试验选取4月龄、体重[（22.75±1.20） kg]相近的杜泊×小尾寒羊杂交一代公羔48只，随机分为4组，每组12只羊，在基础饲粮饲喂基础上分别补饲0（对照组）、10（10GSPs组）、20（20GSPs组）、40 mg/kg BW（40GSPs组）的GSPs。试验期共60 d，其中预试期15 d，正试期45 d。结果表明：1）10GSPs和20GSPs组平均日增重和平均日采食量显著高于对照组和40GSPs组（P<0.05），40GSPs组与对照组差异不显著（P>0.05）。补饲GSPs对料重比影响不显著（P>0.05）。2）GSPs补饲组的附睾重量均显著高于对照组（P<0.05），睾丸、附睾指数在4组间无显著差异（P>0.05）。20GSPs和40GSPs组的顶体完整率显著高于10GSPs组和对照组（P<0.05），精子畸形率显著低于对照组（P<0.05）。3）20GSPs和40GSPs组睾丸、附睾组织中的总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）活性显著高于对照组（P<0.05），GSPs补饲组附睾超氧化物歧化酶（SOD）活性均显著高于对照组（P<0.05），20GSPs和40GSPs组睾丸、附睾组织中丙二醛（MDA）含量显著低于10GSPs和对照组（P<0.05）。4）睾丸组织中，20GSPs组的SOD mRNA相对表达量显著高于10GSPs组和对照组（P<0.05），40GSPs组的谷胱甘肽过氧化物酶4（GPx4）mRNA相对表达量显著高于10GSPs组和对照组（P<0.05），4组间的过氧化氢酶（CAT）和核因子E2相关因子2（Nrf2）mRNA相对表达量差异不显著（P>0.05）；附睾组织中，20GSPs和40GSPs组SOD mRNA相对表达量显著高于对照组（P<0.05），与10GSPs组差异不显著（P>0.05）；GSPs补饲组GPx4 mRNA相对表达量均显著高于对照组（P<0.05）；20GSPs和40GSPs组Nrf2 mRNA相对表达量显著高于对照组（P<0.05）。综上，补饲适量GSPs可以促进绵羊公羔生长，提高睾丸和附睾组织抗氧化能力，进而改善精液品质；在本试验条件下GSPs最适补饲量为20 mg/kg BW。

关键词： 葡萄籽原花青素; 睾丸; 附睾; 精液品质; 抗氧化功能

本文引用格式

牟春堂 , 杨文军 , 王鹏举 , 任国栋 , 剧浩 , 任有蛇 , 郝小燕 , 张建新 . 葡萄籽原花青素对公羔羊生长性能、精液品质及睾丸和附睾抗氧化指标的影响[J]. 动物营养学报, 2020 , 32(5) : 2241 -2250 . DOI: 10.3969/j.issn.1006-267x.2020.05.033

Abstract

The aim of this experiment was to investigate the effects of feeding grape seed procyanidins (GSPs) on growth performance, semen quality, testis and epididymis antioxidant indexes of male lambs. A total of forty-eight 4-month-old Dorper (♂)×small Tail Han sheep (♀) F1 male lambs with similarly weight of (22.75±1.2) kg were randomly selected and divided into 4 groups equally. All the lambs fed a basal diet and supplemented with 0 (control group), 10 (10GSPs group), 20 (20GSPs group) and 40 mg/kg BW (40GSPs group) GSPs, respectively. The experiment was conducted over 60 d, with the first 15 d for adaptation and 45 d for test. The results showed as follows:1)average daily gain (ADG) and average daily feed intake (ADFI) in 10GSPs和20GSPs groups were significantly higher than those in the control group and 40GSPs group (P<0.05), with no significant difference between 40GSPs group and control group (P>0.05). No significant difference was observed in feed conversion ratio among GSPs groups (P>0.05). 2) The weight of epididymis in the GSPs groups was significantly higher than that in the control group (P<0.05), and the indexes of testis and epididymis had no significant difference among the four groups (P>0.05). The acrosome integrity in 20GSPs and 40GSPs groups was significantly higher than that in 10GSPs and control groups (P<0.05), and the rate of sperm malformation in 20GSPs and 40GSPs groups was significantly lower than that in control group (P<0.05). 3) The activity of total antioxidant capacity (T-AOC) and glutathion peroxidase (GSH-Px) in testis and epididymis in 20GSPs and 40GSPs groups were significantly higher than those in control group (P<0.05). The superoxide dismutase (SOD) activity in epididymis in three GSPs groups was significantly higher than that in the contol group (P<0.05), and the malonaldehyde (MDA) content in the testis and epididymis in 20GSPs and 40GSPs groups was significantly lower than that in 10GSPs group and control group (P<0.05). 4) In testis, the relative expression levels of SOD mRNA in 20GSPs group and glutathione peroxidase 4 (GPx4) mRNA in 40GSPs group were significantly higher than those in 10GSPs group and control group (P<0.05). There was no significant difference among four groups in the relative expression levels of catalase (CAT) and nuclear factor E2 correlation factor 2 (Nrf2) mRNA (P>0.05). In epididymis, the relative expression levels of SOD mRNA in 20GSPs and 40GSPs groups was significantly higher than that in control group (P<0.05), but had no significantly difference compared with 10GSPs group (P>0.05). The relative expression level of GPx4 mRNA in three GSPs groups was significantly higher than that in control group (P<0.05). Additionally, the relative expression level of Nrf2 mRNA in 20GSPs and 40GSPs groups was significantly higher than that in control group (P<0.05). In summary, dietary supplementation of GSPs can promote the growth and improve the antioxidant status of testis and epididymis of ram, thereby improving semen quality. The optimal GSPs supplemental level is 20 mg/kg BW under the condition of this experiment.

Key words： grape seed procyanidins; testis; epididymis; semen quality; antioxidant function

参考文献

[1] RASOOLI A,JALALI M T,NOURI M,et al.Effects of chronic heat stress on testicular structures,serum testosterone and cortisol concentrations in developing lambs[J].Animal Reproduction Science,2010,117(1/2):55-59.
[2] 金亚倩,刘文忠,赵俊星,等.酿酒葡萄皮渣对单栏饲养方式下公绵羊繁殖性能及睾丸抗氧化性的影响[J].畜牧兽医学报,2016,47(11):2228-2239.
[3] 施力光,赵春萍,曹婷,等.不同日粮精粗比对海南黑山羊抗氧化性能的影响[J].中国草食动物科学,2015,35(1):29-31.
[4] KIM H S,QUON M J,KIM J A.New insights into the mechanisms of polyphenols beyond antioxidant properties;lessons from the green tea polyphenol,epigallocatechin 3-gallate[J].Redox Biology,2014,2:187-195.
[5] 师维.原花青素对玉米赤霉烯酮诱导小鼠睾丸支持细胞氧化损伤的保护作用[D].硕士学位论文.沈阳:沈阳农业大学,2017.
[6] 厉秀梅.饲养密度与偏热环境对肉鸡骨骼和肌肉生长、氧化及肠道形态的影响[D].硕士学位论文.北京:中国农业科学院,2018.
[7] 侯志高,王振勇,柴同杰,等.不同精粗比日粮对奶牛机体氧化应激和瘤胃内环境稳定性的影响[J].畜牧兽医学报,2008,39(4):455-459.
[8] CALLAGHAN M J,MCAULIFFE P,RODGERS R J,et al.Subacute ruminal acidosis reduces sperm quality in beef bulls[J].Journal of Animal Science,2016,94(8):3215-3228.  
[9] 张晓春,陈指龙,方娟,等.白藜芦醇通过沉默调节蛋白1-解偶联蛋白2信号通路降低小鼠睾丸间质细胞TM3的氧化损伤[J].动物营养学报,2018,30(7):2632-2640.
[10] 常旭红,田敏敏,张琼,等.葡萄籽原花青素对顺铂引起大鼠睾丸损伤的保护机制[C]//中国毒理学会第七次全国会员代表大会暨中国毒理学会第六次中青年学者科技论坛论文摘要.重庆:中国毒理学会,2018.
[11] 王友良,甘功友,张冬初,等.原花青素对反式脂肪酸致雄性小鼠生殖损伤的保护作用[J].食品安全质量检测学报,2018,9(20):5365-5369.
[12] 郑燕,杨明建,申海玉,等.茶多酚对UVB照射雄性小鼠生殖系统的保护作用[J].河北工业大学学报,2012,41(3):19-23.
[13] 李永义,段绪东,赵娇,等.茶多酚对氧化应激仔猪生长性能和免疫功能的影响[J].中国畜牧杂志,2011,47(15):53-57.
[14] 王凯,王洋,孙娟娟,等.苜蓿和红车轴草黄酮提取物对绵羊生长性能和血液相关指标的影响[J].中国兽医学报,2017,37(4):704-709.
[15] 潘发明,李发弟,郝正里,等.茶渣单宁含量对绵羊养分消化利用与氮代谢参数的影响[J].畜牧兽医学报,2012(1):75-85.
[16] ZHAO J X,LI Q,ZHANG R X,et al.Effect of dietary grape pomace on growth performance,meat quality and antioxidant activity in ram lambs[J].Animal Feed Science and Technology,2018,236:76-85.
[17] 金亚倩,郝松华,赵俊星,等.日粮中添加葡萄皮渣对绵羊生长性能、器官指数及血液生化指标的影响[J].中国畜牧兽医,2016,43(9):2326-2332.
[18] 潘发明,李发弟,郝正里,等.茶渣单宁含量对绵羊养分消化利用与氮代谢参数的影响[J].畜牧兽医学报,2012,43(1):71-81.
[19] 赵梦迪,邸凌峰,唐泽宇,等.单宁与饲用纤维素酶对湖羊瘤胃微生物菌群的影响[J].中国畜牧兽医,2019,46(1):112-122.
[20] BITGUL G,TEKMEN I,KELES D,et al.Protective effects of resveratrol against chronic immobilization stress on testis[J].ISRN Urology,2013,2013:278720.
[21] YASSA H A,GEORGE S M,REFAIY A E R M,et al.Camellia sinensis (green tea) extract attenuate acrylamide induced testicular damage in albino rats[J].Environmental Toxicology,2014,29(10):1155-1161.  
[22] 王秋林.机体氧化还原状态的研究[D].博士学位论文.成都:四川大学,2005.
[23] 张建新,王茜,荀文娟,等.GPxs家族基因在山羊不同组织和睾丸不同发育时期的表达特性研究[J].畜牧兽医学报,2011,42(5):650-657.
[24] SNEDDON A A,WU H C,FARQUHARSON A,et al.Regulation of selenoprotein GPx4 expression and activity in human endothelial cells by fatty acids,cytokines and antioxidants[J].Atherosclerosis,2003,171(1):57-65.  
[25] ALVAREZ J G,STOREY B T.Spontaneous lipid peroxidation in rabbit epididymal spermatozoa:its effect on sperm motility[J].Biology of Reproduction,1982,27(5):1102-1108.  
[26] 赵俊星,刘向东,金亚倩,等.日粮中添加酿酒葡萄渣对绵羊肉品质及肌肉抗氧化性的影响[J].畜牧兽医学报,2017,48(8):1481-1490.
[27] ZHAO J X,JIN Y Q,DU M,et al.The effect of dietary grape pomace supplementation on epididymal sperm quality and testicular antioxidant ability in ram lambs[J].Theriogenology,2017,97:50-56.
[28] 任有蛇,秦小伟,郭丽娜,等.日粮纳米锌水平对公羊睾丸和附睾Cu-ZnSOD表达的影响[J].畜牧兽医学报,2014,45(10):1622-1630.
[29] MCMAHON M,ITOH K,YAMAMOTO M,et al.Keap1-dependent proteasomal degradation of transcription factor Nrf2 contributes to the negative regulation of antioxidant response element-driven gene expression[J].Journal of Biological Chemistry,2003,278(24):21592-21600.  
[30] ZHONG R Z,XIAO W J,ZHOU D W,et al.Effect of tea catechins on regulation of cell proliferation and antioxidant enzyme expression in H2O2-induced primary hepatocytes of goat in vitro[J].Journal of Animal Physiology and Animal Nutrition,2013,97(3):475-484.  
[31] 张婷,刘瑞,宋小锋,等.葡萄籽原花青素通过Nrf2/ARE信号通路抗高糖诱导的HUVEC-12细胞氧化损伤[J].天然产物研究与开发,2017,29(8):1265-1269.
[32] 金亚倩.日粮中添加酿酒葡萄皮渣对公绵羊繁殖性能及睾丸、附睾抗氧化性的影响[D].硕士学位论文.晋中:山西农业大学,2016.
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