水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

饲料中添加磺胺甲恶唑和土霉素对团头鲂生长性能、血清生化指标、抗氧化能力和组织结构的影响

  • 孙盛明 ,
  • 陈健翔 ,
  • 朱悦洁 ,
  • 卢泽宇 ,
  • 戈贤平 ,
  • 程磊 ,
  • 石连玉
展开
  • 1. 上海海洋大学农业农村部淡水水产种质资源重点实验室, 上海 201306;
    2. 中国水产科学研究院淡水渔业研究中心, 无锡 214081;
    3. 南京农业大学无锡渔业学院, 无锡 214081;
    4. 农业农村部淡水水产生物技术与遗传育种重点实验室, 中国水产科学研究院黑龙江水产研究所, 哈尔滨 150070
孙盛明(1983-),男,山东莱州人,教授,博士,研究方向为水产动物环境生物学研究。E-mail:sunshengming621416@163.com

收稿日期: 2019-10-08

  网络出版日期: 2020-05-15

基金资助

中央级公益性科研院所基本科研业务费专项资金(2019XT0406);国家大宗淡水鱼类产业技术体系(CARS-46);农业部淡水水产生物技术与遗传育种重点实验室开放课题(2019KF02)

Effects of Dietary Sulfamethoxazole and Oxytetracycline on Growth Performance, Serum Biochemical Indexes, Antioxidant Ability and Tissue Structure of Juvenile Megalobrama amblycephala

  • SUN Shengming ,
  • CHEN Jianxiang ,
  • ZHU Yuejie ,
  • LU Zeyu ,
  • GE Xianping ,
  • CHENG Lei ,
  • SHI Lianyu
Expand
  • 1. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;
    2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;
    3. Wuxi Fishery College Nanjing Agricultural University, Wuxi 214081, China;
    4. Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China

Received date: 2019-10-08

  Online published: 2020-05-15

摘要

本试验旨在探究饲料中添加磺胺甲恶唑(SMZ)和土霉素(OTC)对团头鲂幼鱼生长、血清生化指标、抗氧化能力和组织结构的影响。选择平均体重为(3.20±0.09) g的健康团头鲂幼鱼270尾,随机分成3组,每组3个重复,每个重复30尾鱼。对照组饲喂不添加抗生素的基础饲料,试验组分别在基础饲料中添加0.20%磺胺甲恶唑(SMZ组)和0.25%土霉素(OTC组)。试验期56 d。结果表明:1)与对照组相比,SMZ组和OTC组团头鲂幼鱼的存活率、脏体比和肝体比均无显著差异(P>0.05),但终末体重、增重率、摄食率和饲料效率显著降低(P<0.05)。2)SMZ组和OTC组的血清中谷丙转氨酶和谷草转氨酶活性显著高于对照组(P<0.05),血清中碱性磷酸酶活性显著低于对照组(P<0.05)。3)SMZ组和OTC组的肝脏中超氧化物歧化酶、过氧化氢酶活性和谷胱甘肽含量显著低于对照组(P<0.05),肝脏中丙二醛含量显著高于对照组(P<0.05)。4)SMZ组和OTC组的肠道蛋白酶和脂肪酶活性显著低于对照组(P<0.05)。5)组织切片观察表明,SMZ组和OTC组团头鲂幼鱼肠道组织出现小肠绒毛形状不规则且杯状细胞减少现象,肝脏组织则出现部分细胞胞浆疏松或空泡状现象。综上所述,饲料中添加过量的抗生素会抑制团头鲂幼鱼的生长和抗氧化能力,并不同程度地改变肝脏和肠道组织结构。

本文引用格式

孙盛明 , 陈健翔 , 朱悦洁 , 卢泽宇 , 戈贤平 , 程磊 , 石连玉 . 饲料中添加磺胺甲恶唑和土霉素对团头鲂生长性能、血清生化指标、抗氧化能力和组织结构的影响[J]. 动物营养学报, 2020 , 32(5) : 2316 -2325 . DOI: 10.3969/j.issn.1006-267x.2020.05.041

Abstract

This experiment was conducted to study the effects of dietary sulfamethoxazole (SMZ) and oxytetracycline (OTC) on growth performance, serum biochemical indexes, antioxidant ability and tissue structure of juvenile Megalobrama amblycephala. A total of 270 juvenile Megalobrama amblycephala with an average body weight of (3.20±0.09) g were randomly divided into 3 groups with 3 replicates per group and 30 fish per replicate. Fish in the control group were fed a basal diet without antibiotic, and others in experiment groups were fed the basal diet supplemented 0.20% sulfamethoxazole (SMZ group) and 0.25% oxytetracycline (OTC group), respectively. The experiment lasted for 56 days. The results showed as follows:1) compared with the control group, the survival rate, viscerosomtic index and hepatosomatic index of juvenile Megalobrama amblycephala of SMZ group and OTC group had no significant difference (P>0.05), but the final body weight, weight gain rate, feed rate and feed efficiency rate were significantly decreased (P<0.05). 2) The activities of alanine transaminase and aspartate transaminase in serum of SMZ group and OTC group were significantly higher than those of the control group (P<0.05), and the serum alkaline phosphatase activity was significantly lower than that of the control group (P<0.05). 3) The superoxide dismutase and catalase activities and glutathione content in liver of SMZ group and OTC group were significantly lower than those of the control group (P<0.05), and the liver malondialdehyde content was significantly higher than that of the control group (P<0.05). 4) The activities of protease and lipase in intestine of SMZ group and OTC group were significantly lower than those of the control group (P<0.05). 5) The observation of tissue section showed that the irregular intestinal villus shape and goblet cells decreased in intestinal tissue of juvenile Megalobrama amblycephala, and the partial hepatocytes with cytoplasmic loosening or vacuolation in SMZ group and OTC group. In summary, dietary excessive antibiotic can inhibit the growth and antioxidant ability of juvenile Megalobrama amblycephala, and change the structure in liver and intestine in different degrees.

参考文献

[1] 伊风军.抗生素添加剂在饲料中的应用历史和现状[J].中国畜牧兽医文摘,2017,33(11):221.
[2] 叶文红.抗生素渔药的使用情况及其替代品发展简介[J].河北渔业,2018(12):55-57.
[3] GUO J H,SELBY K,BOXALL A B A.Assessment of the risks of mixtures of major use veterinary antibiotics in European surface waters[J].Environmental Science & Technology,2016,50(15):8282-8289.  
[4] 叶继丹,韩友文,赵吉伟,等.喹乙醇对鲤肝胰脏抗氧化酶系统的影响[J].水产学报,2004,28(3):231-235.
[5] HE S X,WANG Q M,LI S N,et al.Antibiotic growth promoter olaquindox increases pathogen susceptibility in fish by inducing gut microbiota dysbiosis[J].Science China Life Sciences,2017,60(11):1260-1270.  
[6] ZHAO Y,LIU H,WANG Q,et al.The influence of three antibiotics on the growth,intestinal enzyme activities,and immune response of the juvenile sea cucumber Apostichopus japonicus selenka[J].Fish & Shellfish Immunology,2019,84:434-440.
[7] LIMBU S M,ZHOU L,SU S X,et al.Chronic exposure to low environmental concentrations and legal aquaculture doses of antibiotics cause systemic adverse effects in Nile tilapia and provoke differential human health risk[J].Environment International,2018,115:205-219.
[8] 曾巧云,丁丹,檀笑.中国农业土壤中四环素类抗生素污染现状及来源研究进展[J].生态环境学报,2018,27(9):1774-1782.
[9] 肖贺.复方磺胺嘧啶在罗非鱼体内的药代动力学研究[D].硕士学位论文.上海:上海海洋大学,2014:6-8.
[10] 范克俭.土霉素和新诺明在中国明对虾体内的药代动力学研究[D].硕士学位论文.青岛:中国海洋大学,2005:1-13.
[11] 郑增忍,赵思俊,邹明,等.土霉素在大菱鲆体内药代动力学及残留消除规律研究[J].中国农学通报,2008,24(10):559-563.
[12] ELEMA M O,HOFF K A,KRISTENSEN H G.Bioavailability of oxytetracycline from medicated feed administered to Atlantic salmon (Salmo salar L.) in seawater[J].Aquaculture,1996,143(1):7-14.  
[13] 王群,孙修涛,刘德月,等.复方新诺明在鲈鱼体内的药物代谢动力学研究[J].海洋科学,2001,25(2):35-38.
[14] 张石云,宋超,张敬卫,等.微塑料暴露对罗非鱼肌肉中磺胺甲唑残留的影响[J].生态与农村环境学报,2018,34(9):857-864.
[15] 原盛广,崔艳芳,张文婧.北京农贸市场常见淡水鱼体内抗生素残留调查研究[J].生态毒理学报,2015,10(3):311-317.
[16] 任鸣春,周群兰,缪凌鸿,等.团头鲂营养需求与健康研究进展[J].水产学报,2015,39(5):761-768.
[17] 中华人民共和国农业部渔业局.2019年中国渔业统计年鉴[M].北京:中国农业出版社,2019:25-35.
[18] LIU X,STEELE J C,MENG X Z.Usage,residue,and human health risk of antibiotics in Chinese aquaculture:a review[J].Environmental Pollution,2017,223:161-169.
[19] YONAR E M,YONAR M S,SILICI S.Protective effect of propolis against oxidative stress and immunosuppression induced by oxytetracycline in rainbow trout (Oncorhynchus mykiss,W.)[J].Fish & Shellfish Immunology,2011,31(2):318-325.  
[20] 辛福言,李晓川,陈四清,等.高效液相色谱法分析鲤鱼体内土霉素残留的研究[J].海洋水产研究,1996,17(1):71-75.
[21] 张博翔,班龙科,周睿,等.6种磺胺类抗生素的液相色谱法分析条件及优化[J].安徽建筑大学学报,2018,26(6):51-55.
[22] 郑宗林,周玉岩,龚哲宇.几种抗生素对异育银鲫生长性能及饲料利用的影响[J].粮食与饲料工业,2008(4):31-33.
[23] 陈四清,辛福言,李晓川,等.饲喂土霉素对鲤鱼生长及代谢残留的研究[J].饲料工业,1997,18(5):20-21.
[24] 胡俊茹,王国霞,莫文艳,等.黑水虻幼虫粉替代鱼粉对鲈鱼幼鱼生长性能、体组成、血浆生化指标和组织结构的影响[J].动物营养学报,2018,30(2):613-623.
[25] 李娜,赵玉民,陈正元,等.抗生素诱导肠道菌群失调对肠黏膜屏障和肝脏功能的动态影响[J].动物营养学报,2019,31(3):1278-1287.
[26] NYBLOM H,BERGGREN U,BALLDIN J,et al.High AST/ALT ratio may indicate advanced alcoholic liver disease rather than heavy drinking[J].Alcohol and Alcoholism,2004,39(4):336-339.  
[27] 时博,郁欢欢,梁晓芳,等.酵母水解物对大口黑鲈生长性能、血浆生化指标以及肝脏组织健康的影响[J].动物营养学报,2018,30(7):2772-2781.
[28] WINSTON G W,DI GIULIO R T.Prooxidant and antioxidant mechanisms in aquatic organisms[J].Aquatic Toxicology,1991,19(2):137-161.  
[29] 徐永平,汪婷婷,孙永欣,等.水产动物溶菌酶研究的最新进展[J].水产科学,2011,30(5):307-310.
[30] 毕景成.肠碱性磷酸酶在肠屏障中作用的研究进展[J].肠外与肠内营养,2015,22(4):244-247.
[31] 冯建军,姚志刚,张子平,等.大黄鱼碱性磷酸酶基因的克隆及表达特征分析[J].水产学报,2014,38(9):1243-1254.
[32] 黄莹,姚远,朱晓鸣,等.饲喂不同浓度黄曲霉毒素B1饲料对草鱼幼鱼生长和毒素积累的影响[J].水产学报,2019,43(4):723-730.
[33] ZHOU L,LIMBU S M,QIAO F,et al.Influence of long-term feeding antibiotics on the gut health of zebrafish[J].Zebrafish,2018,15(4):340-348.  
[34] 刘玥宏,丁悦,徐敬东.肠道杯状细胞及其功能研究进展[J].世界华人消化杂志,2017,25(14):1279-1286.
[35] KIM A,KIM N,ROH H J,et al.Administration of antibiotics can cause dysbiosis in fish gut[J].Aquaculture,2019,512:734330.
[36] 刘莉,王中康,俞和韦,等.饲喂四环素和链霉素对贡嘎蝠蛾幼虫生长和肠道消化酶的影响[J].昆虫知识,2008,45(2):272-275.
文章导航

/