本试验旨在研究饲粮中锌、锰和植酸酶水平对肉鸡生长性能、血清生化指标以及血清中锌、锰含量的影响，探究饲粮中添加植酸酶是否能提高锌、锰的吸收利用率，从而降低饲粮中锌、锰的添加量。选取14日龄体重接近、健康的爱拔益加（AA）肉鸡696只，随机分成6个组，每组12个重复，每个重复10只鸡。试验采用2×3双因子试验设计，分别在基础饲粮中添加2个水平的植酸酶（0和2 000 FTY/kg）和3个水平的锌、锰组合（30 mg/kg锌+40 mg/kg锰、60 mg/kg锌+70 mg/kg锰、90 mg/kg锌+100 mg/kg锰），共组成6种饲粮。试验期为28 d。结果表明：1）饲粮中添加植酸酶极显著提高了14~28日龄肉鸡的平均日增重（P=0.001），显著提高了29~42日龄肉鸡的平均日增重（P=0.038），显著降低了14~28日龄肉鸡的料重比（P=0.022）。2）饲粮中添加植酸酶显著提高了肉鸡血清中谷丙转氨酶（ALT）和总超氧化物歧化酶（T-SOD）活性（P=0.027、P=0.037），极显著提高了血清中谷草转氨酶（AST）、磷酸肌酸激酶（CPK）和锰超氧化物歧化酶（Mn-SOD）活性（P=0.003、P=0.001、P=0.007）。饲粮中添加锌、锰显著降低了肉鸡血清中尿素氮（UN）含量（P=0.041）。植酸酶和锌、锰的交互作用显著或极显著影响肉鸡血清中CPK和T-SOD活性（P=0.010、P=0.006）。3）饲粮中添加锌、锰显著提高了肉鸡血清中锌含量（P=0.033），饲粮中添加植酸酶显著提高了肉鸡血清中锌含量（P=0.047）。综上所述，饲粮中添加植酸酶能够提高肉鸡生长性能和血清抗氧化酶活性。饲粮中添加锌、锰有利于机体蛋白质的合成。饲粮中添加植酸酶并没有达到降低锌、锰添加量的目的。

The purpose of this study was to investigate the effects of dietary zinc, manganese and phytase levels on growth performance, serum biochemical parameters and serum zinc and manganese contents of broilers, to explore whether dietary phytase can improve the absorption and utilization of zinc and manganese, so as to reduce the supplemental levels of zinc and manganese in diet. A total of 696 fourteen-day-old healthy Arbor Acres (AA) broilers with similar body weight were randomly divided into 6 groups with 12 replicates per group and 10 broilers per replicate. The experiment was based on a 2×3 two factors design, 2 levels of phytase (0 and 2 000 FTY/kg) and 3 levels of zinc and manganese combination (30 mg/kg zinc+40 mg/kg manganese, 60 mg/kg zinc+70 mg/kg manganese, 90 mg/kg zinc+100 mg/kg, respectivly), composed 6 kinds of diets. The experiment lasted for 28 days. The results showed as follows:1) dietary addition of phytase significantly increased the average daily gain of broilers during 14 to 28 days of age (P=0.001), significantly increased the average daily gain of broilers during 29 to 42 days of age (P=0.038), and significantly decreased the feed to gain ratio of broilers during 14 to 28 days of age (P=0.022). 2) Dietary addition of phytase significantly increased the activities of glutamic-pyruvic transaminase (ALT) and total superoxide dismutase (T-SOD) in serum of broilers (P=0.027 and P=0.037), and significantly increased the activities of glutamic oxalacetic transaminase (AST), creatine phosphate kinase (CPK) and manganese superoxide dismutase (Mn-SOD) in serum (P=0.003, P=0.001 and P=0.007). Dietary addition of zinc and manganese significantly decreased the serum urea nitrogen (UN) content in serum of broilers (P=0.041). The interaction of phytase with zinc and manganese significantly affected the activities of CPK and T-SOD in serum of broilers (P=0.010 and P=0.006). 3) Dietary addition of zinc and manganese significantly increased the serum zinc content in serum of broilers (P=0.033), and dietary addition of phytase significantly increased the serum zinc content in serum of broilers (P=0.047). To sum up, dietary addition of phytase can improve the growth performance and serum antioxidant enzyme activities of broilers. Dietary addition of zinc and manganese promote the protein synthesis. Dietary addition of phytase do not reduce the additive amount of zinc and manganese.