反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

瘤胃体外发酵评价红曲米酒糟作为山羊饲料原料的可行性

  • 简仕燕 ,
  • 王菲 ,
  • 郭子義 ,
  • 马政发 ,
  • 陈祥 ,
  • 吴文旋 ,
  • 陈鑫珠 ,
  • 刘景 ,
  • 吴佳海 ,
  • 牟琼
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  • 1. 贵州大学动物科学学院动物营养与饲料科学研究所, 贵阳 550025;
    2. 贵州大学高原山地动物遗传育种与繁殖教育部重点实验室, 贵阳 550025;
    3. 贵州大学新农村发展研究院, 贵阳 550025;
    4. 贵州省山地畜禽养殖污染控制与资源化技术工程实验室, 贵阳 550025;
    5. 福建省农业科学院畜牧兽医研究所, 福州 350013;
    6. 贵州省草业研究所, 贵阳 550006
简仕燕(1994-),女,贵州遵义人,硕士研究生,动物营养与饲料科学专业。E-mail:2270601473@qq.com

收稿日期: 2020-03-21

  网络出版日期: 2020-08-13

基金资助

国家重点研发计划项目(2018YFD0502005);福建省农科院对外合作项目(DEC201910);国家自然科学基金项目(31960674);贵州省高层次科技创新人才计划(黔科合人才[2016]4024)

Evaluation of Red Yeast Rice Distillers' Grains Used as Feed for Goats by Rumen Fermentation in Vitro

  • JIAN Shiyan ,
  • WANG Fei ,
  • GUO Ziyi ,
  • MA Zhengfa ,
  • CHEN Xiang ,
  • WU Wenxuan ,
  • CHEN Xinzhu ,
  • LIU Jing ,
  • WU Jiahai ,
  • MOU Qiong
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  • 1. Institute of Animal Nutrition and Feed Science, College of Animal Science, Guizhou University, Guiyang 550025, China;
    2. Ministry of Education Key Laboratory of Animal Genetics, Breeding and Reproduction of Plateau Mountain Region, Guizhou University, Guiyang 550025, China;
    3. Institute of New Rural Development, Guizhou University, Guiyang 550025, China;
    4. Engineering Key Lab of Plateau Mountain Livestock Pollution Control and Resource Reuse Technology of Guizhou Province, Guiyang 550025, China;
    5. Animal Husbandry and Veterinary Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
    6. Institute of Guizhou Paratulcure Science, Guiyang 550006, China

Received date: 2020-03-21

  Online published: 2020-08-13

摘要

本试验旨在通过体外法研究红曲米酒糟对山羊瘤胃产气量与发酵参数的影响,评价其作为山羊饲料原料的可行性。采集9只山羊的瘤胃液,应用ANKOM RFS产气系统进行体外发酵,其中对照组发酵底物不含红曲米酒糟,试验Ⅰ组、试验Ⅱ组、试验Ⅲ组发酵底物分别含有50、100、150 mg/g的红曲米酒糟。测定指标包括:1、2、3、4、5、6、7、8、9、10、11、12、24、36、48、72 h的产气量(GP)以及体外培养72 h的pH、干物质消失率(DMD)、氨态氮(NH3-N)与挥发性脂肪酸(VFA)浓度、纤维素酶活性。结果显示:1)试验Ⅲ组在1~5 h内产气量显著高于对照组(P<0.05);各组干物质消失率差异不显著(P>0.05);各组发酵液pH均处于正常水平,且氨态氮浓度组间差异不显著(P>0.05)。2)各组发酵液乙酸、丙酸、丁酸、总挥发性脂肪酸浓度与乙酸/丙酸差异不显著(P>0.05)。3)试验Ⅲ组发酵液木聚糖酶、纤维二糖酶活性均显著高于对照组(P<0.05)。由此可见,红曲米酒糟可增加山羊瘤胃体外发酵产气量、提高纤维素酶活性,促进瘤胃发酵,具有作为山羊饲料原料的潜力,值得开展饲养试验确定其在山羊饲粮中的适宜添加量。

本文引用格式

简仕燕 , 王菲 , 郭子義 , 马政发 , 陈祥 , 吴文旋 , 陈鑫珠 , 刘景 , 吴佳海 , 牟琼 . 瘤胃体外发酵评价红曲米酒糟作为山羊饲料原料的可行性[J]. 动物营养学报, 2020 , 32(8) : 3780 -3788 . DOI: 10.3969/j.issn.1006-267x.2020.08.037

Abstract

The purpose of this experiment was to investigate the effects of red yeast rice distillers' grains (RYRDG) on rumen gas production and fermentation parameters of goats in vitro for evaluating the feasibility of RYRDG used as feed for goats. Nine goats were used as the donor of rumen fluid. The ANKOM RFS gas production measurement system was used for in vitro fermentation. Fermentation substances of control group did not contain RYRDG, while the fermentation substances of trial groups Ⅰ, Ⅱ and Ⅲ contained 50, 100 and 150 mg/g RYRDG, respectively. Gas production were determined after 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 24, 36, 48 and 72 h culture, and dry matter disappearance, pH, the concentrations of ammoniacal nitrogen (NH3-N) and volatile fat acids and cellulases activity were determined after 72 h culture. The results showed as follows:1) trial group Ⅲ had significantly higher gas production than control group after 1 to 5 h culture (P<0.05). There was no significant difference in dry matter disappearance among 4 groups (P>0.05). The fermentation broth pH of each group was in the normal level, and the NH3-N concentration had no significant difference among 4 groups (P>0.05). 2) The concentrations of fermentation broth acetate, propionate, butyrate, total volatile fat acids and acetate/propionate were not significant difference among all groups (P>0.05). 3) The activities of fermentation broth xylanase and cellobiase of trial group Ⅲ was significantly increased compared with control group (P<0.05). It is suggested that RYRDG can increase the gas production of rumen fermentation in vitro of goats,enhance the increase cellulase activity, and improve the rumen fermentation, and thus it has potential as feed for goats. Further feeding trial is needed to set the appropriate supplemental level in the diet of goats.

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