动物营养学报 >

2020 , Vol. 32 >Issue 8: 3843 - 3855

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.08.043

分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

基于宏基因组学解析瘤胃微生物调节荷斯坦奶牛乳蛋白含量的研究

  • 吴建民 ,
  • 王雍 ,
  • 周协琛 ,
  • 耿子健 ,
  • 贾大庆 ,
  • 韩杨 ,
  • 王建发 ,
  • 武瑞
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  • 黑龙江八一农垦大学动物科技学院, 黑龙江省牛病防治重点实验室, 大庆 163319
吴建民(1994-),男,黑龙江庆安人,硕士研究生,从事牛乳营养品质调控研究。E-mail:wujianmin1994@163.com

收稿日期: 2020-02-24

  网络出版日期: 2020-08-13

基金资助

国家自然科学基金项目（31402157，31772698）

收起

Rumen Microorganism Regulating Milk Protein Content in Holstein Dairy Cows Based on Metagenomic Analysis

  • WU Jianmin ,
  • WANG Yong ,
  • ZHOU Xiechen ,
  • GENG Zijian ,
  • JIA Daqing ,
  • HAN Yang ,
  • WANG Jianfa ,
  • WU Rui
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  • Key Laboratory of Prevention and Control of Cattle Diseases in Heilongjiang Province, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2020-02-24

  Online published: 2020-08-13

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摘要

本试验旨在研究相同饲粮结构及饲养环境下，基于宏基因组学解析乳蛋白含量与瘤胃微生物之间的潜在关系。以荷斯坦奶牛为研究对象，选择乳蛋白含量长期偏高（>3.7%）、长期偏低（3.0%~3.3%）的奶牛各3头，采集瘤胃内容物，通过宏基因组学方法分析二者之间的潜在调节作用。结果表明：拟杆菌门（Bacteroidetes）、拟杆菌纲（Bacteroidia）、普雷沃氏菌属（Prevotella）、普雷沃氏菌科（Prevotellaceae）、拟杆菌目（Bacteroidales）、栖瘤胃普雷沃氏菌（Prevotella ruminicola）相对含量在2组间呈现显著差异（P<0.05），其中Prevotella ruminicola为主要差异微生物，并首次发现三角酵母属（Trigonopsis）可能对调节乳蛋白含量具有一定作用。在乳蛋白含量长期偏高的奶牛瘤胃内，与乳蛋白前体物生成过程相关的氨基酸代谢和其他氨基酸代谢功能基因的相对丰度显著升高（P<0.05），与蛋白质降解及氨基酸代谢过程密切相关的COG0542、COG0612、COG1404、COG1506、COG2755、COG4870基因的相对丰度显著升高（P<0.05），COG1404基因相对丰度富集度最高，其在微生物降解蛋白质过程中通过表达多肽酶发挥功能。综上，在相同饲粮结构及饲养环境下，瘤胃内Prevotella ruminicola和Trigonopsis这2种微生物相对含量的差异对调节乳蛋白含量具有一定作用；微生物通过高表达COG0542、COG0612、COG1404、COG1506、COG2755、COG4870基因增强瘤胃内蛋白质降解及氨基酸代谢作用，影响乳蛋白前体物合成情况，进而调节乳蛋白的含量。

关键词： 荷斯坦奶牛; 瘤胃微生物; 乳蛋白; 前体物

本文引用格式

吴建民 , 王雍 , 周协琛 , 耿子健 , 贾大庆 , 韩杨 , 王建发 , 武瑞 . 基于宏基因组学解析瘤胃微生物调节荷斯坦奶牛乳蛋白含量的研究[J]. 动物营养学报, 2020 , 32(8) : 3843 -3855 . DOI: 10.3969/j.issn.1006-267x.2020.08.043

Abstract

This experiment was in order to study a potential relationship between the milk protein content and rumen microorganism in dairy cows under the same dietary structure and feeding environment. Three Holstein dairy cows with high milk protein content (>3.7%) for a long time and three Holstein dairy cows with low milk protein content (3.0% to 3.3%) for a long time were selected. Their rumen fluid was collected and their regulatory effects were analyzed by macrogenomics. This experiment showed that Bacteroidetes, Bacteroidia, Prevotella, Prevotellaceae, Bacteroidales, Prevotella ruminicola showed significant differences between the two groups (P<0.05), Prevotella ruminicola was the main difference microorganism. It was found for the first time that Trigonopsis may had a certain effect in regulating milk protein content. There were a significant increase on the relative abundance of functional genes of amino acid metabolism and metabolism of other amino acids in the rumen of dairy cows of high milk protein content for a long time (P<0.05); there were a significant increase on the gene number of COG0542, COG0612, COG1404, COG1506, COG2755 and COG4870 that closely related to protein degradation and amino acid metabolism (P<0.05), and the highest concentration of COG1404 gene was found, which could function through the expression of peptidase. In conclusion, Prevotella ruminicola and Trigonopsis in rumen under the same dietary structure and feeding environment have a certain effect on the regulation of milk protein content. Through high expression of COG0542, COG0612, COG1404, COG1506, COG2755 and COG4870 genes, rumen protein degradation and amino acid metabolism are enhanced, which affect the synthesis of milk protein precursors, and then regulate milk protein content.

Key words： Holstein dairy cows; rumen microorganisms; milk protein; precursor

参考文献

[1] 马美蓉.提高奶牛乳蛋白率的措施[J].饲料研究,2004(8):35-37.
[2] 马露,周凌云,卜登攀,等.日粮营养水平对奶牛乳蛋白合成的影响及其作用机理的研究进展[J].中国畜牧兽医,2012,39(10):121-127.
[3] 张海波.日粮补充棕榈酸对泌乳早期荷斯坦奶牛泌乳性能、血液生化指标和激素水平的影响[J].畜牧兽医学报,2019,50(1):203-210.
[4] LAPIERRE H,MARTINEAU R,HANIGAN M D,et al.Review:impact of protein and energy supply on the fate of amino acids from absorption to milk protein in dairy cows[J].Animal,2020,14(Suppl.1):S87-S102.
[5] ZANG Y,SILVA L H P,GHELICHKHAN M,et al.Incremental amounts of rumen-protected histidine increase plasma and muscle histidine concentrations and milk protein yield in dairy cows fed a metabolizable protein-deficient diet[J].Journal of Dairy Science,2019,102(5):4138-4154.  
[6] 李树聪,王加启.泌乳奶牛小肠可吸收氨基酸来源及其调控[J].中国奶牛,2005(4):14-17.
[7] 刘公明.小肠可消化必需氨基酸的构成对泌乳奶山羊乳蛋白合成的影响[D].硕士学位论文.泰安:山东农业大学,2017.
[8] ZHOU Y,SONG F,LI Y S,et al.Double-antibody based immunoassay for the detection of β-casein in bovine milk samples[J].Food Chemistry,2013,141(1):167-173.  
[9] 陈文亮,毛仁淡.加热对牛乳中β-乳球蛋白(β-LG)的影响[J].中国乳业,2006(2):54-55.
[10] 孙福昱,赵一广,熊本海,等.海带粉对饲喂高精料饲粮奶牛瘤胃发酵参数和菌群结构的影响[J].动物营养学报,2019,31(6):2842-2853.
[11] 牛化欣,常杰,胡宗福,等.基于组学技术研究反刍动物瘤胃微生物及其代谢功能的进展[J].畜牧兽医学报,2019,50(6):1113-1122.
[12] 孙会增.基于系统生物学技术解析饲草质量影响奶牛泌乳性能的生理与代谢机制[D].博士学位论文.杭州:浙江大学,2017.
[13] 田彦.基于高通量测序的中国荷斯坦奶牛瘤胃宏转录组研究[D].博士学位论文.北京:中国科学院北京基因组研究所,2015.
[14] BLASCO C,PICÓ Y,TORRES C M.Progress in analysis of residual antibacterials in food[J].TrAC Trends in Analytical Chemistry,2007,26(9):895-913.  
[15] NASROLLAHI S M,ZALI A,GHORBANI G R,et al.Variability in susceptibility to acidosis among high producing mid-lactation dairy cows is associated with rumen pH,fermentation,feed intake,sorting activity,and milk fat percentage[J].Animal Feed Science and Technology,2017,228:72-82.
[16] 马健,SHAH A M,王之盛.瘤胃微生物区系的影响因素及其调控措施[J].动物营养学报,2020, 32(5):1957-1964.
[17] JAMI E,ISRAEL A,KOTSER A,et al.Exploring the bovine rumen bacterial community from birth to adulthood[J].The ISME Journal,2013,7(6):1069-1079.  
[18] 逄宾宾,王雨,杨妍,等.健康中国荷斯坦奶牛不同发育阶段瘤胃的菌群多样性研究[J].黑龙江畜牧兽医,2018(5):14-18,261.
[19] 李子健,李大彪,高民,等.不同生理阶段荷斯坦奶牛瘤胃细菌多样性研究[J].动物营养学报,2018,30(8):3017-3025.
[20] MALMUTHUGE N,GUAN L L.Understanding host-microbial interactions in rumen:searching the best opportunity for microbiota manipulation[J].Journal of Animal Science and Biotechnology,2017,8(2):300-306.
[21] MARTIN C,FERLAY A,MOSONI P,et al.Increasing linseed supply in dairy cow diets based on hay or corn silage:effect on enteric methane emission,rumen microbial fermentation,and digestion[J].Journal of Dairy Science,2016,99(5):3445-3456.  
[22] XUE M Y,SUN H Z,WU X H,et al.Assessment of rumen bacteria in dairy cows with varied milk protein yield[J].Journal of Dairy Science,2019,102(6):5031-5041.  
[23] HAZLEWOOD G P,ORPIN C G,GREENWOOD Y,et al.Isolation of proteolytic rumen bacteria by use of selective medium containing leaf fraction 1 protein (ribulosebisphosphate carboxylase)[J].Applied and Environmental Microbiology,1983,45(6):1780-1784.  
[24] GRUNINGER R J,RIBEIRO G O,CAMERON A,et al.Invited review:application of meta-omics to understand the dynamic nature of the rumen microbiome and how it responds to diet in ruminants[J].Animal,2019,13(9):1843-1854.  
[25] 赵沁沁,刘军.变异三角酵母D-氨基酸氧化酶基因的克隆与表达[J].武汉工业学院学报,2011,30(1):22-25.
[26] 刘树臣,谢澜漪,李春,等.热带假丝酵母细胞内pH的测定及其与生长代谢活性的关系[J].生物工程学报,2004,20(2):279-283.
[27] 吴浪,卢冬亚,张艳,等.苜蓿干草与苜蓿青贮碳水化合物分子结构特征及其与奶牛瘤胃内细菌含量相关性研究[J].家畜生态学报,2019,40(9):39-44.
[28] LEVASSEUR A,DRULA E,LOMBARD V,et al.Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes[J].Biotechnology for Biofuels,2013,6:41.
[29] RICHARDSON C R,HATFIELD E E.The limiting amino acids in growing cattle[J].Journal of Animal Science,1978,46(3):740-745.  
[30] 刘倩,符潮,周传社,等.反刍动物瘤胃微生物限制性氨基酸代谢研究进展[J].家畜生态学报,2017,38(2):83-87.
[31] 李吕木,乐国伟,施用晖.蛋氨酸为氮、碳源对瘤胃微生物氨基酸代谢的影响[J].西北农林科技大学学报(自然科学版),2006,34(7):22-26.
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