长期缺硒对大鼠体内D-丝氨酸、L-丝氨酸水平的影响

余海立; 张凤伟; 张彦; 张双庆

doi:10.3969/j.issn.1006-267x.2020.08.045

动物营养学报 >

2020 , Vol. 32 >Issue 8: 3869 - 3876

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.08.045

实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

长期缺硒对大鼠体内D-丝氨酸、L-丝氨酸水平的影响

余海立 ,
张凤伟 ,
张彦 ,
张双庆

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1. 酵母功能湖北省重点实验室, 宜昌 443003;
2. 中国疾病预防控制中心营养与健康所, 北京 100050

余海立(1992-),男,安徽六安人,硕士,研究方向为营养与保健。E-mail:yuhl@angelyeast.com

收稿日期: 2020-02-20

网络出版日期: 2020-08-13

基金资助

湖北省自然科学基金（2017CFB572，2018CFB612）

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Effects of Long-Term Selenium Deficiency on D-Serine and L-Serine Levels in Rats

YU Haili ,
ZHANG Fengwei ,
ZHANG Yan ,
ZHANG Shuangqing

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1. The Hubei Provincial Key Laboratory of Yeast Function, Yichang 443003, China;
2. National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China

Received date: 2020-02-20

Online published: 2020-08-13

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摘要

本试验旨在建立缺硒大鼠模型，研究长期缺硒对大鼠体内D-丝氨酸、L-丝氨酸水平的影响。选取3周龄雄性断乳大鼠12只，随机分为2组（每组6只）：缺硒组大鼠饲喂低硒饲粮（硒水平为0.02 mg/kg），对照组大鼠同期饲喂正常饲粮（硒水平为0.18 mg/kg），试验期300 d。利用电感耦合等离子体质谱法测定血浆中硒水平，利用邻苯二甲醛和N-乙酰-L-半胱氨酸柱前衍生，高效液相色谱法测定大鼠体内D-丝氨酸、L-丝氨酸水平。结果表明：1）使用Agilent ZORBAX SB-C18色谱柱（4.6 mm×150 mm，5 μm），采用50 mmol/L醋酸铵和甲醇作为流动相进行梯度洗脱，D-丝氨酸和L-丝氨酸得到很好地分离。2）与饲喂正常饲粮的大鼠相比，饲喂低硒饲粮的大鼠血浆中硒水平极显著降低（P<0.01），血浆中D-丝氨酸、L-丝氨酸以及小脑中L-丝氨酸水平升高，但差异未达显著水平（P>0.05）。由此可见，通过饲喂低硒饲粮可建立缺硒大鼠模型，长期低硒饲粮饲喂对大鼠血浆、小脑中D-丝氨酸、L-丝氨酸水平无显著影响。

关键词： 硒; D-丝氨酸; L-丝氨酸

本文引用格式

余海立 , 张凤伟 , 张彦 , 张双庆 . 长期缺硒对大鼠体内D-丝氨酸、L-丝氨酸水平的影响[J]. 动物营养学报, 2020 , 32(8) : 3869 -3876 . DOI: 10.3969/j.issn.1006-267x.2020.08.045

Abstract

This experiment was conducted to establish a selenium deficiency rat model and investigate effects of long-term selenium deficiency on D-serine and L-serine levels in rats. Twelve male weaned rats aged 3 weeks were randomly divided into 2 groups and each group had 6 rats. Rats in the selenium deficiency group were fed a low selenium diet (selenium level of 0.02 mg/kg) and rats in the control group were fed a normal diet (selenium level of 0.18 mg/kg). The experiment lasted for 300 days. Selenium level in plasma were determined using inductively coupled plasma mass spectrometry. High performance liquid chromatography was used to analyze the levels of D-serine and L-serine after derivatizating with o-phthalaldehyde and N-acetyl-L-cysteine. The results showed as follows:1) under the mobile phase consisting of 50 mmol/L of ammonium acetate and methanol, D-serine and L-serine were successfully separated on the Agilent ZORBAX SB-C18 column (4.6 mm×150 mm, 5 μm). 2) Compared with the rats fed normal diet, plasma selenium level of rats fed selenium deficiency diet was significantly decreased (P<0.01), and plasma D-serine, L-serine levels as well as cerebellum L-serine level were increased, but differences were not significant (P>0.05). In conclusion, the selenium deficiency rat model is successfully established by selenium-deficient diets, and D-serine, L-serine levels in plasma and cerebellum are not significantly affected by the long-term selenium deficiency.

Key words： selenium; D-serine; L-serine

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