艾蒿醇提物对肉仔鸡生长性能及肝脾抗氧化和免疫指标的影响

张晶; 杨硕; 邢媛媛; 杜海东; 江阳; 徐元庆; 金晓; 史彬林

doi:10.3969/j.issn.1006-267x.2020.11.024

动物营养学报 >

2020 , Vol. 32 >Issue 11: 5185 - 5194

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.11.024

禽营养与饲料 POULTRY NUTRITION AND FEED

艾蒿醇提物对肉仔鸡生长性能及肝脾抗氧化和免疫指标的影响

张晶 ,
杨硕 ,
邢媛媛 ,
杜海东 ,
江阳 ,
徐元庆 ,
金晓 ,
史彬林

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内蒙古农业大学动物科学学院, 呼和浩特 010018

张晶(1996-),女,内蒙古包头人,硕士研究生,研究方向为动物环境与营养。E-mail:jingyuer96@163.com

收稿日期: 2020-04-29

网络出版日期: 2020-11-16

基金资助

国家自然科学基金项目（31660674）

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Effects of Artemisia argyi Ethanol Extract on Growth Performance, Immunity and Antioxidant Indexes in Liver and Spleen of Broilers

ZHANG Jing ,
YANG Shuo ,
XING Yuanyuan ,
DU Haidong ,
JIANG Yang ,
XU Yuanqing ,
JIN Xiao ,
SHI Binlin

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College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2020-04-29

Online published: 2020-11-16

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摘要

本试验旨在研究艾蒿醇提物（AAEE）对肉仔鸡生长性能及肝脾抗氧化和免疫指标的影响。选取240只1日龄爱拔益加（AA）肉仔鸡，采用完全随机试验设计，随机分成5组，在基础饲粮中分别添加0（对照）、250、500、750和1 000 mg/kg AAEE，每组6个重复，每个重复8只鸡（公母各占1/2），试验期42 d。结果表明：1）42日龄时，750 mg/kg AAEE添加组的肉仔鸡末体重极显著高于对照组（P<0.01），且有一次线性升高趋势（P=0.094）；500和750 mg/kg AAEE添加组的总采食量显著低于对照组（P<0.05），且有显著的一次线性（P=0.071）或二次曲线（P=0.075）降低趋势。2）42日龄时，与对照组相比，添加500和750 mg/kg AAEE显著提高了肝脏过氧化氢酶（CAT）活性（P<0.05），极显著提高了总抗氧化能力（T-AOC）（P<0.01），且2项指标均随AAEE添加量的增加呈显著的一次线性（P<0.05）或极显著的二次曲线（P<0.01）升高效应。21日龄时，与对照组相比，添加750 mg/kg AAEE分别极显著提高了脾脏总超氧化物歧化酶（T-SOD）、CAT活性和T-AOC （P<0.01），且三者均有极显著的一次线性或二次曲线升高效应（P<0.01）。42日龄时，与对照组相比，添加750 mg/kg AAEE极显著提高了脾脏CAT活性（P<0.01），显著提高了T-AOC （P<0.05），显著降低丙二醛（MDA）含量（P<0.05），此外CAT活性具有极显著的一次线性或二次曲线升高效应（P<0.01）。3）42日龄时，与对照组相比，添加500和750 mg/kg AAEE极显著降低了肝脏白细胞介素-1β（IL-1β）含量（P<0.01），显著降低白细胞介素-6（IL-6）含量（P<0.05），且二者均有极显著的二次曲线降低效应（P<0.01）。21日龄时，与对照组相比，添加500和750 mg/kg AAEE分别显著降低了脾脏IL-1β和IL-6含量（P<0.05），此外IL-1β具有显著的二次曲线降低效应（P<0.05）；添加750和1 000 mg/kg AAEE同时极显著升高脾脏白细胞介素-2（IL-2）和白细胞介素-4（IL-4）含量（P<0.01），且二者均有极显著的一次线性或二次曲线升高效应（P<0.01）。42日龄时，添加750 mg/kg AAEE使脾脏IL-1β含量显著低于对照组（P<0.05），且具有显著的二次曲线降低效应（P<0.05）。综上所述，750 mg/kg的AAEE添加量更有利于AA肉仔鸡生长，提高其抗氧化和免疫功能。

关键词： 艾蒿醇提物; 生长性能; 免疫指标; 抗氧化指标

本文引用格式

张晶 , 杨硕 , 邢媛媛 , 杜海东 , 江阳 , 徐元庆 , 金晓 , 史彬林 . 艾蒿醇提物对肉仔鸡生长性能及肝脾抗氧化和免疫指标的影响[J]. 动物营养学报, 2020 , 32(11) : 5185 -5194 . DOI: 10.3969/j.issn.1006-267x.2020.11.024

Abstract

The aim of this study was to investigate the effects of Artemisia argyi ethanol extract (AAEE) on growth performance, immunity and antioxidant indexes in liver and spleen of broilers. Using completely randomized trial design, two hundred and forty Arbor Acres broilers (1 day of age) were selected and randomly allotted to 5 groups, and each replicate with 8 broilers (half males and half females). Basal diet supplemented with 0 (control), 250, 500, 750, and 1 000 mg/kg of AAEE were constituted the experiment diets, respectively. The experiment lasted for 42 days. The results showed as follows: 1) at 42 days of age, the final body weight of broilers in 750 mg/kg AAEE group was significantly higher than that in the control group (P<0.01) and increased linearly (P=0.094). Adding 500 and 750 mg/kg AAEE significantly decreased the total feed intake (FI) compared with the control group (P<0.05). Moreover, total FI linearly (P=0.071) or quadratically decreased (P=0.075) with the supplementation of AAEE. 2) At 42 days of age, dietary supplementation of 500 and 750 mg/kg AAEE significantly increased catalase (CAT) activity in liver (P<0.05) and significantly increased total antioxidant capacity (T-AOC) (P<0.01) compared with the control group, and both of them linearly (P<0.05) or quadratically (P<0.01) increased in the liver. In spleen of broilers, compared with the control group, adding 750 mg/kg AAEE significantly increased the T-AOC and the activities of CAT and total superoxide dismutase (T-SOD) (P<0.01). And all above indexes linearly or quadratically (P<0.01) increased at 21 days of age. Adding 750 mg/kg AAEE significantly increased CAT activity (P<0.01) and T-AOC (P<0.05) in spleen, and significantly decreased malondialdehyde (MDA) content (P<0.05) compared with the control group at 42 days of age. In addition, CAT linearly or quadratically increased (P<0.01). 3) In the liver of broilers, 500 and 750 mg/kg AAEE significantly reduced interleukin-1β (IL-1β) (P<0.01) and interleukin-6 (IL-6) (P<0.05) contents in liver compared with the control group, and both of them quadratically decreased (P<0.01) at 42 days of age. In the spleen of broilers, compared with the control group, adding 500 and 750 mg/kg AAEE significantly reduced IL-1β and IL-6 contents (P<0.05) at 21 days of age. In addition, IL-1β contents quadratically decreased (P<0.05). Compared with the control group, adding 750 and 1 000 mg/kg AAEE significantly increased interleukin-2 (IL-2) and interleukin-4 (IL-4) (P<0.01) contents, and both of them linearly or quadratically increased (P<0.01) at 21 days of age. Adding 750 mg/kg AAEE, IL-1β content was not only significantly lower than that in the control group (P<0.05), but also quadratically decreased (P<0.05) at 42 days of age. In conclusion, the addition of 750 mg/kg AAEE is more beneficial to the growth and improvement of antioxidant and immune function of broilers.

Key words： Artemisia argyi ethanol extract; growth performance; immune index; antioxidant index

参考文献

[1] 辛翔飞,郑麦青,文杰,等.2019年肉鸡产业形势分析、未来展望与对策建议[J].中国畜牧杂志,2020,56(3):155-159.
[2] 刘超齐,常娟,王平,等.艾草的生物学功能及在畜牧生产上的应用[J].动物营养学报,2018,30(9):3417-3422.
[3] 张鹏飞.艾蒿水提物对脂多糖刺激的肉仔鸡免疫和抗氧化功能的影响及其机理研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2018.
[4] CORRÊA-FERREIRA M L,FERREIRA D M,DALLAZEN J L,et al.Gastroprotective effects and structural characterization of a pectic fraction isolated from Artemisia campestris subsp maritima[J].International Journal of Biological Macromolecules,2018,107:2395-2403.
[5] KANG J R,KANG M J,CHOI M H,et al.Physicochemical characteristics of ethanol extract from artemisia Argyi H.using different preparation methods[J].Journal of Life Science,2017,27(1):23-31.

[6] SIDDIQUI A,AKHTAR J,SHAHAB U M S,et al.A naturally occurring flavone (chrysin):chemistry,occurrence,pharmacokinetic,toxicity,molecular targets and medicinal properties[J].Journal of Biologically Active Products from Nature,2018,8(4):208-227.

[7] 杨硕,邢媛媛,郭世伟,等.不同采收期艾蒿水提物体外抗氧化活性的研究[J].饲料研究,2019,42(6):83-86.
[8] 范建平.两种绿绒蒿属植物醇提物抑制白血病细胞增殖效应的生物学机制研究[D].博士学位论文.西安:陕西师范大学,2018.
[9] SONG X W,WEN X,HE J W,et al.Phytochemical components and biological activities of Artemisia argyi[J].Journal of Functional Foods,2019,52:648-662.
[10] 杨硕,徐元庆,邢媛媛,等.植物源黄酮类化合物对动物免疫和抗氧化功能影响的研究进展[J].动物营养学报,2019,31(7):2958-2964.
[11] ZHOU Y F,MAO S G,ZHOU M X.Effect of the flavonoid baicalein as a feed additive on the growth performance,immunity,and antioxidant capacity of broiler chickens[J].Poultry Science,2019,98(7):2790-2799.

[12] CAI L M,ZHANG X S,HOU M L,et al.Natural flavone tricetin suppresses oxidized LDL-induced endothelial inflammation mediated by Egr-1[J].International Immunopharmacology,2020,80:106224.
[13] 杨洁,曹旭蓉,林佳丽,等.黄杨叶中黄酮多糖和多酚含量及其抗氧化活性[J].农业与技术,2018,38(15):22-24.
[14] 胡林峰,崔乘幸,吴玉博,等.艾蒿化学成分及其生物活性研究进展[J].河南科技学院学报(自然科学版),2010,38(4):75-78.
[15] 曹振兴.艾蒿粉对肉仔鸡生产性能和免疫功能的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2016.
[16] 楚维斌.艾蒿水提物对肉仔鸡生产性能和免疫功能的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2016.
[17] 牛壮.艾蒿多糖对肉仔鸡生长性能及肠道相关指标的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2019.
[18] 周明星.基于Nrf2信号通路的天然抗氧化剂研究[D].硕士学位论文.济南:山东大学,2018.
[19] 陈宏燕.艾蒿粉对肉仔鸡抗氧化和免疫功能及相关基因表达量的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2017.
[20] 赵飞.艾蒿水提物对肉仔鸡免疫和抗氧化功能及相关基因表达的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2017.
[21] NOVIDIYANTO N,FARMAWATI A,LESTARI L A.Pengaruh pemberian kecambah kacang hijau[Phaseolus radiatus (L.)] terhadap kadar malondealdehid (MDA) plasma dan jaringan hati tikus Sprague Dawley yang diberi pakan lemak tinggi[J].Journal Gizi Klinik Indonesia,2017,13(2):82-89.
[22] 高温婷,王金荣,苏兰利,等.畜禽抗氧化应激过程中核因子E2相关因子2信号通路的调控作用机制[J].饲料研究,2020,43(4):137-140.
[23] 杨汝才,杨胜林,谭斌,等.饲粮中添加辣木黄酮对蛋雏鸭生长、免疫及抗氧化功能的影响[J].中国家禽,2019,41(1):31-34.
[24] 李莹.DHM对雏鸡生长和免疫功能影响及其缓解LPS致肝脾损伤作用[D].博士学位论文.哈尔滨:东北农业大学,2019.
[25] 张婷婷,姚镜.离子通道在免疫细胞和免疫应答反应中的功能研究进展[J].生理学报,2019,71(6):894-904.
[26] 门连超,赵勤,何树梅,等.藏药兔耳草醇提物对小鼠免疫功能的影响[J].现代免疫学,2017,37(4):335-341.
[27] QUINTANS-JÚNIOR L J,GANDHI S R,PASSOS F R S,et al.Dereplication and quantification of the ethanol extract of Miconia albicans (melastomaceae) by HPLC-DAD-ESI-/MS/MS,and assessment of its anti-hyperalgesic and anti-inflammatory profiles in a mice arthritis-like model:Evidence for involvement of TNF-α,IL-1β and IL-6[J].Journal of Ethnopharmacology,2020,258:112938.
[28] 董建新,刘晓光,李晨曦,等.酸枣叶醇提物对小鼠巨噬细胞M1和M2型极化的影响[J].天然产物研究与开发,2020,32(6):961-967.

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