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蝉花多糖和植物提取物对雁荡麻鸡生长性能和免疫功能的影响

  • 李瑞萍 ,
  • 刘长国 ,
  • 董丽艳 ,
  • 王秋霞 ,
  • 徐时湘 ,
  • 李国勤 ,
  • 田勇 ,
  • 柴一秋 ,
  • 刘素贞 ,
  • 卢立志
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  • 1. 浙江农林大学动物科技学院, 杭州 311300;
    2. 浙江省农业科学院畜牧兽医研究所, 杭州 310021;
    3. 温州科技职业学院动物科学学院, 温州 325000;
    4. 浙江省亚热带作物研究所, 温州市虫生真菌资源研究与开发重点实验室, 温州 325005
李瑞萍(1993-),女,浙江温州人,硕士研究生,研究方向为家禽遗传与育种研究。E-mail:243870832@qq.com

收稿日期: 2020-03-27

  网络出版日期: 2020-11-16

基金资助

浙江省公益基础研究项目"蝉花虫草提取物在鸡无抗生素饲养中的研究和应用"(LGN19C170004);浙江省农科院仙居鸡产业团队科技特派员项目

Effects of Cordyceps cicadae Polysaccharide and Plant Extract on Growth Performance and Immune Function of Yandang Ma-Chicken

  • LI Ruiping ,
  • LIU Zhangguo ,
  • DONG Liyan ,
  • WANG Qiuxia ,
  • XU Shixiang ,
  • LI Guoqin ,
  • TIAN Yong ,
  • CHAI Yiqiu ,
  • LIU Suzhen ,
  • LU Lizhi
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  • 1. College of Animal Science and Technology, Zhejiang A&F University, Hangzhou 311300, China;
    2. Animal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    3. College of Animal Science, Wenzhou Vocational College of Science and Technology, Wenzhou 325000, China;
    4. Key Laboratory of Entomogenous Fungus Resources Research and Development of Wenzhou, Zhejiang Institute of Subtropical Crops, Wenzhou 325005, China

Received date: 2020-03-27

  Online published: 2020-11-16

摘要

本试验旨在探讨蝉花多糖和植物提取物对雁荡麻鸡生长性能和免疫功能的影响。随机选取同一批次的1日龄雁荡麻鸡雏鸡315只,随机分为7组,分别为空白对照组(A组)、免疫对照组(B组)、抗生素对照组(C组)、蝉花多糖组(D组)、蝉花多糖植物提取物低剂量复合组(E组)、蝉花多糖植物提取物高剂量复合组(F组)和植物提取物组(G组),每组3个重复,每个重复15只。分别于11~17日龄和25~31日龄每天1次灌服试验鸡,其中A组和B组各灌服生理盐水0.5 mL/只,C组灌服杆菌肽锌0.5 mL/只(50 mg/kg BW),D~G组分别灌服蝉花多糖0.5 mL/只(50 mg/kg BW)、蝉花多糖(25 mg/kg BW)+植物提取物(125 mg/kg BW)0.5 mL/只、蝉花多糖(50 mg/kg BW)+植物提取物(125 mg/kg BW)0.5 mL/只和植物提取物0.5 mL/只(250 mg/kg BW)。试验期42 d。结果显示:1)10~28日龄,D~G组雁荡麻鸡平均日增重均显著高于B组(P<0.05);与B组、C组相比,G组料重比有所降低(P>0.05)。2)29~42日龄,D组、F组雁荡麻鸡平均日增重显著高于B组、C组(P<0.05)。3)42日龄,与B组相比,D组、E组雁荡麻鸡体重显著提高(P<0.05)。4)F组、G组雁荡麻鸡血清免疫球蛋白A含量显著高于A组、C组(P<0.05),F组雁荡麻鸡血清免疫球蛋白G含量显著高于A~C组(P<0.05),D组、F组雁荡麻鸡血清补体3含量显著高于C组(P<0.05)。5)与A组、B组相比,E组、G组雁荡麻鸡血清干扰素-γ、肿瘤坏死因子-α、白细胞介素-2含量均显著提高(P<0.05),D组、F组雁荡麻鸡血清白细胞介素-4含量显著高于A~C组(P<0.05)。综上可知,蝉花多糖、植物提取物及其复合物能促进雁荡麻鸡生长,提高其免疫力。本试验条件下,蝉花多糖(50 mg/kg BW)+植物提取物(125 mg/kg BW)高剂量复合组效果最佳。

本文引用格式

李瑞萍 , 刘长国 , 董丽艳 , 王秋霞 , 徐时湘 , 李国勤 , 田勇 , 柴一秋 , 刘素贞 , 卢立志 . 蝉花多糖和植物提取物对雁荡麻鸡生长性能和免疫功能的影响[J]. 动物营养学报, 2020 , 32(11) : 5433 -5440 . DOI: 10.3969/j.issn.1006-267x.2020.11.050

Abstract

This study investigated the effects of Cordyceps cicadae polysaccharide and plant extract on the growth performance and immune function of Yandang Ma-chicken. A total of 315 Yandang Ma-chicks of 1-day-old randomly selected from the same batch were randomly divided into 7 groups (each group containing 3 replicates and 15 chicks in each replicate) as blank control group (group A), immune control group (group B), antibiotics control group (group C), Cordyceps cicadae polysaccharide group (group D), the low-dose compound of Cordyceps cicadae polysaccharide and plant extract group (group E), the high-dose compound of Cordyceps cicadae polysaccharide and plant extract group (group F), and the plant extract group (group G). The chickens were drenched once per day at the ages of 11 to 17 days and 25 to 31 days, respectively. Group A and group B received 0.5 mL normal saline per bird, group C was administrated with 0.5 mL bacitracin zinc per bird (50 mg/kg BW), group D to group G were administrated with 0.5 mL Cordyceps cicadae polysaccharide per bird (50 mg/kg BW), 0.5 mL Cordyceps cicadae polysaccharide (25 mg/kg BW)+plant extract (125 mg/kg BW) per bird, 0.5 mL Cordyceps cicadae polysaccharide (50 mg/kg BW)+plant extract (125 mg/kg BW) per bird, and 0.5 mL plant extract (250 mg/kg BW) per bird, respectively. The trial lasted for 42 days. The results showed as follows: 1) from 10 to 28 days of age, the average daily gain of Yandang Ma-chicken in group D to group G was significantly higher than that in group B (P<0.05), and compared with group B and group C, the feed to gain ratio in group G was reduced (P>0.05). 2) From 29 to 42 days of age, the average daily gain of Yandang Ma-chicken in group D and group F was significantly higher than that in group B and group C (P<0.05). 3) At the age of 42 days, compared with group B, the body weight of Yandang Ma-chicken in group D and group E was significantly increased (P<0.05). 4) The serum immunoglobulin A content of Yandang Ma-chicken in group F and group G was significantly higher than that in group A and group C (P<0.05), the serum immunoglobulin G content of Yandang Ma-chicken in group F was significantly higher than that in group A to group C (P<0.05), and the serum complement 3 content of Yandang Ma-chicken in group D and group F was significantly higher than that in group C (P<0.05). 5) Compared with group A and group B, the serum contents of interferon-γ, tumor necrosis factor-α and interleukin-2 of Yandang Ma-chicken in group E and group G were all significantly increased (P<0.05), and the serum IL-4 content of Yandang Ma-chicken in group D and group F was significantly higher than that in group A to group C (P<0.05). To sum up, the results show that Cordyceps cicadae polysaccharide, plant extract and their compound can promote the growth and enhance the immunity of Yandang Ma-chicken. Under the condition of this experiment, the high-dose combination group of Cordyceps cicadae polysaccharide (50 mg/kg BW) and (125 mg/kg BW) has the best effect.

参考文献

[1] 刘素贞,厉晓腊,涂国众,等.虫草蝉花废弃培养基对肉鸡免疫因子及生长发育的影响[J].中国家禽,2012,34(9):64-65.
[2] 李龙宇,葛言琪,伍一炜,等.蝉花多糖的研究进展[J].现代中药研究与实践,2019,33(4):83-86.
[3] 秦文平,慕程,殷世鹏.中药蝉花的化学成分及药理作用研究进展[J].中国中医药图书情报杂志,2019,43(4):73-76.
[4] 孔华,许丽娟,白宇,等.蝉花虫草粗多糖对鸡新城疫抗体水平影响的研究[J].黑龙江畜牧兽医,2017(15):211-214.
[5] HE J,HE Y X,PAN D D,et al.Associations of gut microbiota with heat stress-induced changes of growth,fat deposition,intestinal morphology,and antioxidant capacity in ducks[J].Frontiers in Microbiology,2019,10:903.
[6] 厉晓腊,陈官菊,方鸣,等.蝉花虫草多糖脱蛋白研究[J].浙江农业科学,2018,59(11):2009-2012.
[7] 李贞明,张贝贝,余苗,等.植物提取物的生物学功能及其在肉鸡生产中的应用进展[J].广东农业科学,2019,46(6):110-117.
[8] 方磊涵,王振,王留,等.中药复方多糖对肉仔鸡生长性能和免疫功能的影响[J].中国兽医杂志,2018,54(9):56-59.
[9] ZHU N H,WANG J,YU L F,et al.Modulation of growth performance and intestinal microbiota in chickens fed plant extracts or virginiamycin[J].Frontiers in Microbiology,2019,10:1333.doi:10.3389/fmicb.2019.01333.
[10] 梁龙华.复合微生态制剂、复合植物提取物、碱性负离子液在不同阶段生长育肥猪日粮中的应用研究[D].硕士学位论文.南宁:广西大学,2017.
[11] 吴春琴,孙思维,干贤通,等.雁荡麻鸡遗传资源调查与分析[J].中国家禽,2017,39(2):67-70.
[12] 杜泓明,汪晓东,王晓磊,等.不同益生菌培养物对断奶仔猪生长性能、免疫功能及血液生化指标的影响[J].中国兽医学报,2017,37(7):1379-1384,1400.
[13] 马铁华,马立,贾荣玲.植物提取物对肉鸡生长性能、养分消化率及盲肠微生物组成的影响[J].中国饲料,2018(20):46-51.
[14] 赵利军,张梅,王炼,等.中药复方对动物机体免疫功能影响的研究进展[J].畜牧与饲料科学,2014,35(2):95-98.
[15] 李树鹏.黄芪多糖益生菌合生元对雏鸡生长和免疫作用的研究[D].硕士学位论文.杨凌:西北农林科技大学,2005.
[16] 郭欣怡,张曼,韩飞,等.复合微生态制剂与黄芪多糖对鸡免疫器官指数和新城疫-禽流感免疫效果的影响[J].中国农学通报,2016,32(32):20-24.
[17] 王琳,赵国先,冯志华,等.白术多糖、枯草芽孢杆菌对育成期蛋鸡生长性能和血清免疫指标的影响[J].中国家禽,2017,39(16):31-35.
[18] 朱永刚,王磊,崔东安,等.植物精油在畜禽生产中的应用效果研究进展[J].中国畜牧兽医,2016,43(7):1812-1817.
[19] 王兰,陈代文,余冰,等.植物精油对肉鸡生长性能、抗氧化能力和免疫机能的影响[J].动物营养学报,2019,31(2):831-838.
[20] 邢帅兵,陈代文,余冰,等.枯草芽孢杆菌对断奶仔猪生长性能和肠道形态、黏膜免疫及菌群数量的影响[J].动物营养学报,2020,32(5):2066-2073.
[21] 李蕴玉,李佩国,张艳英,等.白细胞介素-2(IL-2)在兽医中的应用进展(综述)[J].河北科技师范学院学报,2006,20(4):63-66.
[22] 李立科,罗启慧,黄超,等.大豆异黄酮对雄性大鼠脾脏IL-2、IL-4、TNF-α、INF-γ蛋白表达的影响[J].浙江农业学报,2017,29(9):1458-1464.
[23] SCHRODER K,HERTZOG P J,RAVASI T,et al.Interferon-γ:an overview of signals,mechanisms andfunctions[J].Journal of Leukocyte Biology,2004,75(2):163-189.  
[24] 邹云.植物多糖对断奶仔猪生长性能和免疫功能的影响[D].硕士学位论文.长沙:湖南农业大学,2014.
[25] 刘宽辉,田卫军,高珍珍,等.多糖-硒化多糖复方增强鸡免疫功能的比较研究[J].南京农业大学学报,2017,40(3):521-528.
[26] 王海,王建发,贺显晶,等.缺硒对雏鸡空肠和回肠IFN-γ、IL-2、IL-4基因转录的影响[J].中国兽医学报,2019,39(2):276-280.
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