动物营养学报 >

2020 , Vol. 32 >Issue 11: 5458 - 5471

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.11.053

研究简报 SHORT COMMUNICATION

不同猫粮对猫应激刺激后生理指标和血液指标的影响

  • 王朝好 ,
  • 孙皓然 ,
  • 刘晓雅 ,
  • 尹伊娅 ,
  • 王行健 ,
  • 吴仕龙 ,
  • 魏豪斌 ,
  • 许源玲 ,
  • 冷军 ,
  • 尹文辉 ,
  • 王军军 ,
  • 胡长敏
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  • 1. 华中农业大学动物医院, 武汉 430070;
    2. 中国农业科学院特产研究所, 长春 130112;
    3. 深圳市红狗宠物营养研究院, 深圳 518117
王朝好(1997-),男,安徽六安人,硕士研究生,从事小动物临床研究。E-mail:845540231@qq.com

收稿日期: 2020-04-11

  网络出版日期: 2020-11-16

基金资助

营养学评价项目（2018）；湖北省教改项目（2017191）

收起

Effects of Different Cat Food on Physiological Indexes and Blood Indexes of Cats after Stress Stimulation

  • WANG Chaohao ,
  • SUN Haoran ,
  • LIU Xiaoya ,
  • YIN Yiya ,
  • WANG Xingjian ,
  • WU Shilong ,
  • WEI Haobin ,
  • XU Yuanling ,
  • LENG Jun ,
  • YIN Wenhui ,
  • WANG Junjun ,
  • HU Changmin
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  • 1. Animal Hospital of Huazhong Agricultural University, Wuhan 430070, China;
    2. Institute of Special Animal and Plant Sciences, Chinese Academy of Agriculture Sciences, Changchun 130112, China;
    3. Shenzhen Red Dog Pet Nutrition Institute, Shenzhen 518117, China

Received date: 2020-04-11

  Online published: 2020-11-16

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摘要

本试验旨在研究不同猫粮对猫应激刺激后基础生理指标和血液生理、生化、抗氧化等指标的影响。选择12只健康且体重相近的英国短毛猫，随机分为对照组（CON组）和试验组（CAS组），每组6只。CON组饲喂市售成猫商品粮，CAS组饲喂缓解应激刺激全价处方猫粮，2组给予相同的运输及慢性适度应激刺激及饲养条件。试验测定应激前后及试验结束时采食量、生理指标和血液指标。试验期为3周。结果表明：1）试验成功建立了猫应激刺激模型，与适应性饲喂期相比，应激造模期及观察期2组猫采食量显著下降（P<0.05）。2）2组间体重、体温、心率及呼吸频率差异不显著（P>0.05）。3）2组间血液白细胞数目、红细胞数目、血红蛋白浓度、淋巴细胞数目及嗜中性粒细胞数目差异不显著（P>0.05）；与第0天（应激造模前）相比，第7天CAS组血液白细胞数目显著降低（P<0.05），红细胞数目和血红蛋白浓度显著升高（P<0.05）。4）与CON组相比，第14天CAS组血清乳酸脱氢酶活性、甘油三酯含量显著降低（P<0.05），与第0天相比，第7天CAS组血清尿素氮、甘油三酯含量显著下降（P<0.05），第14天CAS组血清肌酐（P<0.05）、尿素氮含量（P<0.01）及乳酸脱氢酶活性（P<0.05）显著或极显著下降。5）2组间血清超氧化物歧化酶、谷胱甘肽过氧化物酶活性及丙二醛含量差异不显著（P>0.05），与第0天相比，第7天CAS组血清超氧化物歧化酶、谷胱甘肽过氧化物酶活性极显著升高（P<0.01）。6）2组间血清皮质醇含量差异不显著（P>0.05）。由此可见，本试验中全价处方猫粮能及时补充应激时机体代谢的营养消耗，达到缓解应激的目的，是一种可以应用于猫临床应激预防和抗应激治疗的营养解决方案。

关键词： 猫; 缓解应激; 处方粮; 生理指标; 宠物临床营养

本文引用格式

王朝好 , 孙皓然 , 刘晓雅 , 尹伊娅 , 王行健 , 吴仕龙 , 魏豪斌 , 许源玲 , 冷军 , 尹文辉 , 王军军 , 胡长敏 . 不同猫粮对猫应激刺激后生理指标和血液指标的影响[J]. 动物营养学报, 2020 , 32(11) : 5458 -5471 . DOI: 10.3969/j.issn.1006-267x.2020.11.053

Abstract

This experiment was conducted to investigate the effects of different cat food on basal physiological indexes and blood physiological, biochemical, antioxidant indexes after stress stimulation. Twelve healthy British shorthairs with similar body weight were randomly divided into a control group (CON group) and trial group (CAS group), and each group had 6 cats. CON group was fed a commercial cat food, while CAS group was fed stress relieve clinical cat food, and the same chronic moderate stress stimulation and feeding conditions were given. Mean feed intake, basic physiological indexes and blood indexes were measured before and after stress, also at the end of the experiment. The experimental period was 3 weeks. The results showed as follows: 1) the cats’ stress stimulation model was successfully established. Compared with the adaptive feeding period, the feed intake of the two groups decreased significantly during the stress modeling period and the observation period (P<0.05). 2) There were no significant differences in body weight, body temperature, heart rate and respiratory rate between two groups (P>0.05). 3) There were no significant differences in the number of white blood cells, red blood cells, hemoglobin concentration, lymphocyte number and neutrophil number between the two groups (P>0.05). Compared with day 0 (before modeling), the number of white blood cells in CAS group on day 7 was significantly decreased (P<0.05), while the number of red blood cells and hemoglobin concentration were significantly increased (P<0.05). 4) Compared with CON group, the activity of lactate dehydrogenase and triglyceride content in serum in CAS group were significantly decreased on day 14 (P<0.05). Compared with day 0, the contents of urea nitrogen and triglyceride in serum in CAS group were significantly decreased on day 7 (P<0.05). And the contents of creatinine (P<0.05), urea nitrogen (P<0.01), lactate dehydrogenase activity (P<0.05) in serum in CAS group were significantly decreased on day 14. 5) There was no significant difference in the activities of superoxide dismutase and glutathione peroxidase, malondialdehyde content in serum between two group (P>0.05). Compared with day 0, the activities of superoxide dismutase and glutathione peroxidase in serum in CAS group were significantly increased on day 7 (P<0.01). 6) There was no significant difference in serum cortisol content between two groups (P>0.05). In summary, in this study complete cat prescription food can timely supplement the nutrient consumption of body metabolism during stress and achieve the purpose of relieving stress. It is a nutritional solution that can be applied to the clinical anti-stress prevention and treatment of cats.

Key words： cat; relieve stress; prescription food; physiological index; clinical nutrition of pet

参考文献

[1] ROMERO L M,PLATTS S H,SCHOECH S J,et al.Understanding stress in the healthy animal-potential paths for progress[J].Stress,2015,18(5):491-497.  
[2] SOLOMON D A,KELLER M B,LEON A C,et al.Multiple recurrences of major depressive disorder[J].American Journal of Psychiatry,2000,157(2):229-233.  
[3] LOWE S E,BRADSHAW J W S.Ontogeny of individuality in the domestic cat in the home environment[J].Animal Behaviour,2001,61(1):231-237.  
[4] ROCHLITZ I.Feline welfare issues[M]//TURNER D C,BATESON P.The domestic cat.Cambridge:Cambridge University Press,2000:131-153.
[5] BEAVER B V.Feline grooming behavior[M]//BEAVER B V.Feline behavior:a guide for veterinarians.2nd ed.Saunders,St Louis:Elsevier,2003.
[6] BUFFINGTON C A T,WESTROPP J L,CHEW D J,et al.Risk factors associated with clinical signs of lower urinary tract disease in indoor-housed cats[J].Journal of the American Veterinary Medical Association,2006,228(5):722-725.  
[7] TANAKA A,WAGNER D C,KASS P H,et al.Associations among weight loss,stress,and upper respiratory tract infection in shelter cats[J].Journal of the American Veterinary Medical Association,2012,240(5):570-576.  
[8] BEATA C,BEAUMONT E,COLL V,et al.Effect of alpha-casozepine (Zylkene) on anxiety in cats[J].Journal of Veterinary Behavior,2007,2(2):40-46.  
[9] 顾景范,杜寿玢,郭长江.现代临床营养学[M].2版.北京:科学出版社,2009.
[10] 谭艳芳.复合抗应激剂对舍饲条件下猪的抗应激效果的研究[D].硕士学位论文.长沙:湖南农业大学,2003.
[11] 李昊阳,钟荣珍,杨连玉.评价应激的方法及抗应激添加剂的研究进展[J].中国畜牧杂志,2014,50(13):95-99.
[12] KIM K Y,CHOI Y H,HOSSEINDOUST A,et al.Evaluation of high nutrient diets and additional dextrose on reproductive performance and litter performance of heat-tressed lactating sows[J].Animal Science Journal,2019,90(9):1212-1219.  
[13] 孙凤莉,李英,刘荣昌,等.抗热应激剂对奶牛泌乳性能、乳成分及血清指标的影响[C]//中国畜牧兽医学会动物营养学分会第十次学术研讨会论文集.杭州:中国畜牧兽医学会动物营养学分会,2008.
[14] 郭春华,柴映青,王康宁.高温对不同体重生长猪采食量影响模式的研究[J].养猪,2004(4):15-18.
[15] 李绍钰,张敏红,张子仪,等.热应激对肉用仔鸡生产性能及生理生化指标的影响[J].华北农学报,2000,15(3):140-144.
[16] 邢漫萍,杨少雄,林大捷,等.运输性应激对文昌鸡生理指标的影响[J].家禽科学,2016(4):9-10.
[17] 魏光河,张培晏.运输对荣昌猪生理生化指标的影响[J].动物医学进展,2011,32(10):123-127.
[18] SRIKANDAKUMAR A,JOHNSON E H.Effect of heat stress on milk production,rectal temperature,respiratory rate and blood chemistry in Holstein,Jersey and Australian milking Zebu cows[J].Tropical Animal Health and Production,2004,36(7):685-692.  
[19] 单强,马峰涛,金宇航,等.富铬酵母对热应激奶牛生产性能、血清指标及血浆和牛奶中微量元素含量的影响[J].动物营养学报,2020,32(5):2198-2208.
[20] 马峰涛,单强,金宇航,等.金银花提取物对热应激奶牛生产性能、血清生化指标和免疫功能的影响[J].动物营养学报,2020,32(5):2209-2217.
[21] 黄光远,赵川,杨晓炼,等.急性持续热应激对成年兔血液部分生理生化指标的影响[J].安徽农业科学,2015,43(32):69-71,153.
[22] 马永生,董艳娇,马雄,等.运输应激对甘南牦牛血液生理生化指标的影响[J].畜牧兽医杂志,2016,35(5):20-22.
[23] 王雪莹,王之盛,薛白,等.烟酸对热应激牦牛生长性能、营养物质表观消化率和血液指标的影响[J].动物营养学报,2020,32(5):2228-2240.
[24] 范超,吴勇,刘孝然,等.断奶应激对奶水牛犊牛血液生理生化指标和免疫功能的影响[J].中国畜牧兽医,2016,43(7):1749-1754.
[25] 赵恩军,华修国,张斌,等.冷热刺激对犬血清皮质醇、促肾上腺皮质激素及血液生理指标的影响[J].畜牧兽医学报,2003,34(5):457-460.
[26] 高维平,姜桂苗,梁伟超,等.运输应激对驴血液指标的影响[J].中国畜牧兽医,2019,46(2):373-379.
[27] 杨焕民,李士泽.动物冷应激的研究进展[J].黑龙江畜牧兽医,1999(3):3-5.
[28] EARLEY B,FISHER A D,O'RIORDAN E G.Effects of pre-transport fasting on the physiological responses of young cattle to 8-hour road transport[J].Irish Journal of Agricultural and Food Research,2006,45(1):51-60.
[29] NOHARA M,TOHEI A,SATO T,et al.Evaluation of response to restraint stress by salivary corticosterone levels in adult male mice[J].Journal of Veterinary Medical Science,2016,78(5):775-780.  
[30] 肖海霞,托乎提·阿及德,石国庆,等.不同月龄断奶应激对马驹血清指标和体增重的影响[J].畜牧兽医学报,2015,46(11):2010-2019.
[31] KNOWLES T G,BROWN S N,EDWARDS J E,et al.Effect on young calves of a one-hour feeding stop during a 19-hour road journey[J].Veterinary Record,1999,144(25):687-692.  
[32] 邓红雨,范佳英,郑立,等.禁食禁水长途运输和运输后恢复对肉牛血液指标的影响[J].中国兽医学报,2012,32(12):1866-1871,1885.
[33] SWANSON J C,MORROW-TESCH J.Cattle transport:historical,research,and future perspectives[J].Journal of Animal Science,2001,79(Suppl.E):E102-E109.
[34] 芦春莲,李妍,曹玉凤,等.肉牛宰前运输应激对其血液理化指标及免疫机能的影响[J].中国兽医学报,2016,36(7):1173-1177.
[35] 高得仪,韩博,王清兰,等.猪运输应激血液生化指标变化[J].中国兽医学报,1996,16(3):285-289.
[36] 韩瑾瑾,杨高丰,张凯韩,等.夏南牛运输前后生理指标和血液指标的对比研究[J].动物医学进展,2011,32(3):121-124.
[37] 翁恺麒,张扬,陈博雯,等.运输应激和宰前休息对肉鸭血液指标和肉品质的影响[J].中国家禽,2017,39(14):37-40.
[38] DAS R,SAILO L,VERMA N,et al.Impact of heat stress on health and performance of dairy animals:a review[J].Veterinary World,2016,9(3):260-268.  
[39] SONG X Z,LUO J R,FU D B,et al.Traditional Chinese medicine prescriptions enhance growth performance of heat stressed beef cattle by relieving heat stress responses and increasing apparent nutrient digestibility[J].Asian-Australasian Journal of Animal Sciences,2014,27(10):1513-1520.  
[40] 曾涵芳,李烨青,王泽栋,等.黄芪多糖对热应激下奶牛生产性能、血清生化指标的影响[J].中国奶牛,2019(12):42-49.
[41] COMA J,CARRION D,ZIMMERMAN D R.Use of plasma urea nitrogen as a rapid response criterion to determine the lysine requirement of pigs[J].Journal of Animal Science,1995,73(2):472-481.  
[42] 王玲,罗宗刚,蔡明成,等.热应激对肉牛血清离子、酶活性、抗氧化及免疫指标的影响[J].西南师范大学学报(自然科学版),2017,42(12):37-41.
[43] BALNAVE DBRYDEN W L.Advances in the amino acid nutrition of broilers[J].Asian-Australasian Journal of Animal Sciences,2000,13:73.
[44] 孙皓然.饲粮精氨酸水平对蓝狐生长性能和免疫功能的影响[D].硕士学位论文.北京:中国农业科学院,2016.
[45] YU S,PAETAU-ROBINSON I.Dietary supplements of vitamins E and C and β-carotene reduce oxidative stress in cats with renal insufficiency[J].Veterinary Research Communications,2006,30(4):403-413.  
[46] 李玉丹,杨保奎,杨榛,等.不同化学形式微量元素对热应激奶牛生产性能和血清指标的影响[J].动物营养学报,2015,27(11):3558-3566.
[47] SAEKI K,YUO A,TAKAKU F.Cell-cycle-regulated phosphorylation of cAMP response element-binding protein:identification of novel phosphorylation sites[J].Biochemical Journal,1999,338:49-54.
[48] 朱骞,秦海斌,温海,等.运输应激对拉布拉多犬血液皮质醇和生化指标的影响[J].天津农学院学报,2018,25(2):39-41.
[49] CASE L P,DARISTOTLE L,HAYEK M G,et al.Canine and feline nutrition:a resource for companion animal professionals[M].3rd ed.Amsterdam:Elsevier,2011.
[50] 邓华彬,周佳,唐超,等.血球蛋白粉和水解羽毛粉在犬猫粮上的应用研究[J].饲料与畜牧,2019(8):59-64.
[51] 朱惠玲,刘玉兰,陈少魁,等.鱼油提高LPS应激仔猪小肠屏障功能、抑制CRH/CRHR1信号通路[C]//中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集.武汉:中国畜牧兽医学会动物营养学分会,2016.
[52] 美国动物营养国家研究委员会犬猫营养特别委员会组.犬猫营养需要[M].丁丽敏,夏兆飞译.北京:中国农业大学出版社,2010.
[53] 李欣.广州市宠物猫饮食状况与疾病关系的调查及中药处方粮的辅助治疗作用[D].硕士学位论文.广州:华南农业大学,2016.
[54] 陈代文,吴德,张克英,等.猪抗病营养理论与实践[M].北京:中国农业大学出版社,2012.
[55] 文利新,邬静,李荣芳,等.疫苗免疫应激临床观察及其机理研究[C]//中国畜牧兽医学会家畜内科学分会2009年学术研讨会论文集.青岛:中国畜牧兽医学会家畜内科学分会,2009.
[56] 齐莎日娜.猪β-防御素基因表达特点及精氨酸的调节作用[D].博士学位论文.成都:四川农业大学,2010.
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