禽营养与饲料 POULTRY NUTRITION AND FEED

植物乳杆菌对大恒肉鸡生长性能、屠宰性能以及血清免疫和抗氧化功能的影响

  • 宋小燕 ,
  • 彭涵 ,
  • 余春林 ,
  • 陈家磊 ,
  • 杨礼 ,
  • 胡陈明 ,
  • 张增荣 ,
  • 邱莫寒 ,
  • 夏波 ,
  • 熊霞 ,
  • 蒋小松 ,
  • 杜华锐 ,
  • 李晴云 ,
  • 杨朝武
展开
  • 1. 四川省畜牧科学研究院, 动物遗传育种四川省重点实验室, 成都 610066;
    2. 四川大恒家禽育种有限公司, 成都 610066
宋小燕(1982-),女,湖南衡阳人,助理研究员,硕士,主要从事家禽营养研究。E-mail:babalasxy@163.com

收稿日期: 2020-07-07

  网络出版日期: 2021-02-04

基金资助

四川省基本科研业务专项(SASA201903);四川省科技项目(2016NYZ0025,2018NZDZX0004,2019YFN0009);四川省财政运行专项(SASA2020CZYX002,SASA2020CZYX007)

Effects of Lactobacillus plantarum on Growth Performance, Slaughter Performance, Serum Immune and Antioxidant Function of Dahen Broilers

  • SONG Xiaoyan ,
  • PENG Han ,
  • YU Chunlin ,
  • CHEN Jialei ,
  • YANG Li ,
  • HU Chenming ,
  • ZHANG Zengrong ,
  • QIU Mohan ,
  • XIA Bo ,
  • XIONG Xia ,
  • JIANG Xiaosong ,
  • DU Huarui ,
  • LI Qingyun ,
  • YANG Chaowu
Expand
  • 1. Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, China;
    2. Sichuan Daheng Poultry Breeding Company, Chengdu 610066, China

Received date: 2020-07-07

  Online published: 2021-02-04

Supported by

 

摘要

本文旨在研究植物乳杆菌对大恒肉鸡生长性能、屠宰性能以及血清免疫和抗氧化功能的影响。选择健康的1日龄大恒商品代肉雏鸡2 400只,公母各占1/2,分开饲养,公鸡和母鸡各随机分为5个组,每个组6个重复,每个重复40只鸡。对照组(Ⅰ组)饲喂基础饲粮,Ⅱ组、Ⅲ组、Ⅳ组和Ⅴ组分别在基础饲粮中添加1×105、1×106、1×107和1×108 CFU/kg植物乳杆菌,试验期70 d。结果表明:1)与对照组相比,Ⅳ组母鸡期末体重显著提高(P<0.05),其余各试验组与对照组相比差异不显著(P<0.05)。各组公鸡、母鸡平均日采食量、平均日增重和料重比差异均不显著(P>0.05),但随着植物乳杆菌添加水平的提高,料重比呈下降趋势。2)各组公鸡、母鸡屠宰性能差异均不显著(P>0.05)。3)Ⅴ组公鸡血清免疫球蛋白G (IgG)含量显著高于对照组、Ⅱ组、Ⅲ组和Ⅳ组(P<0.05),血清免疫球蛋白A (IgA)和干扰素-γ含量显著高于Ⅱ组、Ⅲ组和Ⅳ组(P<0.05),血清免疫球蛋白M和白细胞介素-2含量显著高于Ⅱ组和Ⅲ组(P<0.05);Ⅴ组母鸡血清IgA和IgG含量显著高于对照组和Ⅱ组(P<0.05)。4)Ⅴ组公鸡血清谷胱甘肽过氧化物酶(GSH-Px)活性最高,显著高于Ⅱ组和Ⅲ组(P<0.05),血清总超氧化物歧化酶活性显著高于Ⅲ组(P<0.05);Ⅴ组母鸡血清GSH-Px活性最高,显著高于Ⅲ组和Ⅳ组(P<0.05);各组公鸡、母鸡血清丙二醛含量差异均不显著(P>0.05)。综上所述,饲粮添加高水平的植物乳杆菌能显著提高大恒肉鸡血清免疫功能,一定程度上增强血清抗氧化功能,对生长性能和屠宰性能无显著影响。

本文引用格式

宋小燕 , 彭涵 , 余春林 , 陈家磊 , 杨礼 , 胡陈明 , 张增荣 , 邱莫寒 , 夏波 , 熊霞 , 蒋小松 , 杜华锐 , 李晴云 , 杨朝武 . 植物乳杆菌对大恒肉鸡生长性能、屠宰性能以及血清免疫和抗氧化功能的影响[J]. 动物营养学报, 2021 , 33(2) : 822 -831 . DOI: 10.3969/j.issn.1006-267x.2021.02.023

Abstract

The present study was designed to investigate the effects of Lactobacillus plantarum on growth performance, slaughter performance, serum immune and antioxidant function of Dahen broilers. A total of 2 400 healthy one-day-old Dahen commercial broiler chickens (half male, half female and raised separately) were selected and cocks and hens were both randomly divided into 5 groups with 6 replicates per group and 40 broilers per replicate, respectively. Broilers in the control group (group Ⅰ) were fed a basal diet, and those in groups Ⅱ, Ⅲ, Ⅳ and Ⅴ were fed the basal diets supplemented with 1×105, 1×106, 1×107 and 1×108 CFU/kg Lactobacillus plantarum, respectively. The experiment lasted for 70 days. The results showed as follows: 1) the final body weight of hens in group Ⅴ was significantly increased compared with the control group (P<0.05), while there was no significant difference between the other experimental groups and the control group (P>0.05). There was no significant difference in average daily feed intake, average daily gain and feed to gain ratio among groups both in cocks and hens (P>0.05), but it could be seen that the feed to gain ratio was decreasing with the increase of Lactobacillus plantarum supplemental level. 2) There was no significant difference in slaughter performance among different groups both in cocks and hens (P>0.05). 3) The serum immunoglobulin G (IgG) content of cocks in group Ⅴ was significantly higher than that in the control group and groups Ⅱ, Ⅲ and Ⅳ (P<0.05), the serum contents of immunoglobulin A (IgA) and interferon-γ (INF-γ) of cocks in group Ⅴ were significantly higher than those in groups Ⅱ, Ⅲ and Ⅳ (P<0.05), and the serum contents of immunoglobulin M and interleukin-2 of cocks in group Ⅴ were significantly higher than those in groups Ⅱ and Ⅲ (P<0.05). The serum contents of IgA and IgG of hens in group Ⅴ were significantly higher than those in the control group and group Ⅱ (P<0.05). 4) The serum glutathione peroxidase (GSH-Px) activity of cocks in group Ⅴ was the highest and significantly higher than that in groups Ⅱ and Ⅲ (P<0.05), the serum total superoxide dismutase activity of cocks in group Ⅴ was significantly higher than that in group Ⅲ (P<0.05). The serum GSH-Px activity of hens in group Ⅴ was the highest and significantly higher than that in groups Ⅲ and Ⅳ (P<0.05). There was no significant difference in serum malonaldehyde content among different groups both in cocks and hens. In summary, the diet supplemented with high level of Lactobacillus plantarum can improve the serum immune function and enhance the serum antioxidant function in a certain extent, while has no significant effect on growth performance and slaughter performance of Dahen broilers.

参考文献

[1] LIAO X D,MA G,CAI J,et al.Effects of Clostridium butyricum on growth performance,antioxidation,and immune function of broilers[J].Poultry Science,2015,94(4):662-667.  
[2] PARK Y H,HAMIDON F,RAJANGAN C,et al.Application of probiotics for the production of safe and high-quality poultry meat[J].Korean Journal for Food Science of Animal Resources,2016,36(5):567-576.  
[3] 田志梅,蒋宗勇,王丽,等.益生菌的功能及其在生猪养殖产业中的应用[J].动物营养学报,2019,31(3):1020-1030. TIAN Z M,JIANG Z Y,WANG L,et al.Function and application of probiotics in pig breeding[J].Chinese Journal of Animal Nutrition,2019,31(3):1020-1030.(in Chinese)
[4] 谢文惠.复合益生菌制剂对肉鸡生长、免疫功能及肠道菌群的影响[D].硕士学位论文.大庆:黑龙江八一农垦大学,2018. XIE W H.Effects of compound probiotics on growth,immunity indices and intestinal flora in broilers[D].Master's Thesis.Daqing:Heilongjiang Bayi Agricultural University,2018.(in Chinese)
[5] 吴宇薇,杨海明,张泉.饲粮添加益生菌对肉鸡免疫机能的影响[J].中国家禽,2019,41(12):43-46. WU Y W,YANG H M,ZHANG Q.Effect of dietary probiotics on immune function of broilers[J].China Poultry,2019,41(12):43-46.(in Chinese)
[6] 马千里,刘冬,顾瑞霞.植物乳杆菌的益生特性及其在乳制品中的应用[J].中国奶牛,2014(1):36-40. MA Q L,LIU D,GU R X.Probiotic properties of lactobacillus plantarum and its application in dairy products[J].China Dairy Cattle,2014(1):36-40.(in Chinese)
[7] 张龙林,刘婧媛.植物乳杆菌在动物生产中的应用[J].湖南饲料,2017(4):30-31. ZHANG L L,LIU J Y.Application of Lactobacillus plantarum in animal production[J].Hunan Feed,2017(4):30-31.(in Chinese)
[8] 张光磊,廖奇,江书忠,等.植物乳杆菌在肉鸡生长中的应用研究进展[J].饲料博览,2019(5):35-37. ZHANG G L,LIAO Q,JIANG S Z,et al.Research progress of application of Lactobacillus plantarum in broiler growth[J].Feed Review,2019(5):35-37.(in Chinese)
[9] 胡宗福,牛化欣,于建华,等.饲料中添加植物乳杆菌对细鳞鲑生长及肠道菌群多样性的影响[J].动物营养学报,2020,32(1):346-356. HU Z F,NIU H X,YU J H,et al.Effects of Lactobacillus plantarum supplementation on growth and intestinal microbial flora diversity of Brachymystax lenok[J].Chinese Journal of Animal Nutrition,2020,32(1):346-356.(in Chinese)
[10] PIEPER R,JANCZYK P,URUBSCHUROV V,et al.Effect of a single oral administration of Lactobacillus plantarum DSMZ 8862/8866 before and at the time point of weaning on intestinal microbial communities in piglets[J].International Journal of Food Microbiology,2009,130(3):227-232.  
[11] 中华人民共和国农业部.NY/T 823-2004家禽生产性能名词术语和度量统计方法[S].北京:中国农业出版社,2004. The Ministry of Agriculture of the People's Republic of China.NY/T 823-2004 Terms and methods of measurement and statistics for poultry production performance[J].Beijing:China Agriculture Press,2004.(in Chinese)
[12] 陆银,武旭峰,费拥军,等.复合益生菌对肉仔鸡的生长性能及屠体品质的影响[J].中国畜牧杂志,2013,49(1):50-53. LU Y,WU X F,FEI Y J,et al.Effect of compound probiotics on growth performance and carcass quality of broilers[J].Chinese Journal of Animal Science,2013,49(1):50-53.(in Chinese)
[13] SHOKRYAZDAN P,JAHROMI M F,LIANG J B,et al.Effects of a Lactobacillus salivarius mixture on performance,intestinal health and serum lipids of broiler chickens[J].PLoS One,2017,12(5):e0175959.
[14] 郭欣怡,张曼,韩飞,等.不同益生菌制剂对肉鸡生产性能、免疫功能和肠道菌群的影响[J].家畜生态学报,2016,37(11):79-83. GUO X Y,ZHANG M,HAN F,et al.Effects of different probiotics on production performance,immunologic function and gut bacteria of broiler chickens[J].Journal of Domestic Animal Ecology,2016,37(11):79-83.(in Chinese)
[15] 县怡涵,赵秀英,李晨博,等.植物乳杆菌和干酪乳杆菌对仔猪生长性能、器官指数及小肠形态的影响[J].动物营养学报,2015,27(12):3805-3811. XIAN Y H,ZHAO X Y,LI C B,et al.Effects of Lactobacillus plantarum and Lactobacillus casei on growth performance,organ index and intestinal morphology of piglets[J].Chinese Journal of Animal Nutrition,2015,27(12):3805-3811.(in Chinese)
[16] 蔡中梅.饲粮中添加巨大芽孢杆菌制剂对仔鹅生产性能、养分利用及肠道菌群的影响[D].硕士学位论文.扬州:扬州大学,2016. CAI Z M.Effect of Bacillus megatherium on the production performance,nutrient utilization and intestinal microflora of geese from 1 to 70 days of age[D].Master's Thesis.Yangzhou:Yangzhou University,2016.(in Chinese)
[17] 黄金华,李泰佑,王士长,等.复合益生菌制剂对肉鸡生长性能、屠宰性能和肌肉品质的影响[J].畜牧与饲料科学,2014,35(5):30-33. HUANG J X,LI T Y,WANG S C,et al.Effects of complex-probiotic-preparation on growth performance,slaughter performance and meat quality of broiler[J].Animal Husbandry and Feed Science,2014,35(5):30-33.(in Chinese)
[18] 贾志新.丁酸梭菌对樱桃谷肉鸭生长性能、免疫和抗氧化功能及肠道食糜VFA含量的影响[D].硕士学位论文.南京:南京农业大学,2014. JIA Z X.Effects of Clostridium butyricum on the growth performance,immune and antioxidant function and the intestinal tract chyme VFA concentration in cherry valley ducks[D].Master's Thesis.Nanjing:Nanjing Agricultural University,2014.(in Chinese)
[19] 徐基利.不同乳酸菌及其添加量对肉仔鸡生长性能、盲肠菌群和免疫功能的影响[D].硕士学位论文.哈尔滨:东北农业大学,2011. XU J L.Effects of different Lactobacillus strains and dosages on growth performance,cecal microflora and immunity of broilers[D].Master's Thesis.Harbin:Northeast Agricultural University,2011.(in Chinese)
[20] 甄玉国,秦贵信,赵巍,等.植物乳杆菌及其培养物对肉仔鸡生长性能和免疫功能的影响[J].中国畜牧杂志,2019,52(13):68-72. ZHEN Y G,QIN G X,ZHAO W,et al.Effects of Lactobacillus plantarum and its culture on growth performance and immune function in broilers[J].Chinese Journal of Animal Science,2019,52(13):68-72.(in Chinese)
[21] LIU H,ZHANG J,ZHANG S H,et al.Oral administration of Lactobacillus fermentum I5007 favors intestinal development and alters the intestinal microbiota in formula-fed piglets[J].International Journal of Food Microbilogy,2014,62(4):860-866.
[22] 田浪,何彦侠,侯月娥,等.复合益生菌制剂对黄羽肉鸡生产性能、抗氧化指标及免疫功能的影响[J].中国兽医学报,2017,37(8):1540-1544,1582. TIAN L,HE Y X,HOU Y E,et al.Effects of dietary mixed probiotics on the growth performance,antioxidant activity and immune function in yellow-feathered broilers[J].Chinese Journal of Veterinary Science,2017,37(8):1540-1544,1582.(in Chinese)
[23] 袁文华,李国勤,韩安法,等.益生菌对青年鸽生长、免疫和抗氧化性能及繁殖相关基因表达的影响[J].动物营养学报,2019,31(7):3294-3301. YUAN W H,LI G Q,HAN A F,et al.Effects of probiotics on growth,immunity,antioxidation and expression of genes related to reproduction of young pigeons[J].Chinese Journal of Animal Nutrition,2019,31(7):3294-3301.(in Chinese)
[24] 廖秀冬.丁酸梭菌的筛选及其对动物抗氧化能力和肉鸡肉品质影响的研究[D].博士学位论文.北京:中国农业大学,2015. LIAO X D.Screening of Clostridium butyricum and its effects on animal antioxidation and meat quality in broiler chickens[D].Ph.D. Thesis.Beijing:China Agricultural University,2015.(in Chinese)
[25] 吴凡,奚雨萌,唐波,等.不同蛋白水平日粮中添加益生素及有机酸复合制剂对青脚麻鸡免疫及抗氧化功能的影响[J].畜牧与兽医,2016,48(1):10-15. WU F,XI Y Y,TANG B,et al.Effects of dietary mixed probiotics and organic acids with different crude protein levels on the immune function and antioxidant activity in green feet broilers[J].Animal Husbandry & Veterinary Medicine,2016,48(1):10-15.(in Chinese)
文章导航

/