禽营养与饲料 POULTRY NUTRITION AND FEED

丁酸梭菌和枯草芽孢杆菌对肉鹅生长性能、消化酶活性、抗氧化功能和肠道形态的影响

  • 于洁 ,
  • 范雪 ,
  • 赵敏孟 ,
  • 董飚 ,
  • 王健 ,
  • 龚道清
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  • 1. 扬州大学动物科学与技术学院, 扬州 225009;
    2. 江苏农牧科技职业学院, 泰州 225300
于洁(1996-),女,山西晋中人,硕士研究生,研究方向为家禽生产。E-mail:yuj126315@163.com

收稿日期: 2020-07-05

  网络出版日期: 2021-02-04

基金资助

江苏省农业科技自主创新资金项目(CX(18)1004);扬州市科技局项目(YZ2018131);泰州市农业科技支撑项目(TN201802)

Effects of Clostridium butyricum and Bacillus subtilis on Growth Performance, Digestive Enzyme Activities, Antioxidant Function and Intestinal Morphology of Meat Geese

  • YU Jie ,
  • FAN Xue ,
  • ZHAO Minmeng ,
  • DONG Biao ,
  • WANG Jian ,
  • GONG Daoqing
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  • 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China

Received date: 2020-07-05

  Online published: 2021-02-04

Supported by

 

摘要

本试验旨在研究饲粮中添加丁酸梭菌和枯草芽孢杆菌对1~70日龄扬州鹅生长性能、消化酶活性、抗氧化功能和肠道形态的影响。选择1日龄健康、体重相近的扬州鹅公鹅288只,随机分为4组,每组6个重复,每个重复12只鹅。A组(对照组)饲喂基础饲粮,B组在基础饲粮中添加250 mg/kg丁酸梭菌(有效活菌数为3.0×106 CFU/g),C组在基础饲粮中添加250 mg/kg枯草芽孢杆菌(有效活菌数为2.0×107 CFU/g),D组在基础饲粮中添加250 mg/kg丁酸梭菌+250 mg/kg枯草芽孢杆菌。试验期10周。结果表明:1) B、C和D组总增重显著高于对照组(P<0.05),C、D组总采食量显著高于对照组(P<0.05)。2) B、C和D组空肠脂肪酶活性显著高于对照组(P<0.05)。3) C、D组血清和回肠总抗氧化能力显著高于对照组(P<0.05)。D组空肠谷胱甘肽含量显著高于对照组(P<0.05),C、D组空肠总超氧化物歧化酶活性显著高于对照组(P<0.05)。D组回肠谷胱甘肽过氧化物酶活性显著高于对照组(P<0.05)。4) C组空肠绒毛高度/隐窝深度(V/C)显著高于对照组(P<0.05)。B、C和D组回肠绒毛高度显著高于对照组(P<0.05),C、D组回肠V/C显著高于对照组(P<0.05)。由此可见,饲粮中添加丁酸梭菌和枯草芽孢杆菌及二者混合制剂可提高肉鹅总增重、空肠脂肪酶活性及抗氧化功能,添加枯草芽孢杆菌可更好地改善肠道形态。

本文引用格式

于洁 , 范雪 , 赵敏孟 , 董飚 , 王健 , 龚道清 . 丁酸梭菌和枯草芽孢杆菌对肉鹅生长性能、消化酶活性、抗氧化功能和肠道形态的影响[J]. 动物营养学报, 2021 , 33(2) : 860 -868 . DOI: 10.3969/j.issn.1006-267x.2021.02.027

Abstract

This study aimed to investigate the effects of Clostridium butyricum and Bacillus subtilis on growth performance, digestive enzyme activities, antioxidant function and intestinal morphology of Yangzhou geese aged from 1 to 70 days. A total of 288 healthy one-day-old Yangzhou male geese with similar body weight were randomly divided into 4 groups with 6 replicates in each group and 12 geese in each replicate. Geese in group A (control group) were fed a basal diet, geese in group B were fed the basal diet supplemented with 250 mg/kg Clostridium butyricum (effective viable count was 3.0×106 CFU/g), geese in group C were fed the basal diet supplemented with 250 mg/kg Bacillus subtilis (effective viable count was 2.0×107 CFU/g), and geese in group D were fed the basal diet supplemented with 250 mg/kg Clostridium butyricum+250 mg/kg Bacillus subtilis. The experiment period was 10 weeks. The results showed as follows:1) the total weight gain of groups B, C and D was significantly higher than that of the control group (P<0.05), and the total feed intake of groups C and D was significantly higher than that of the control group (P<0.05). 2) The jejunal lipase activity of groups B, C and D was significantly higher than that of the control group (P<0.05). 3) The total antioxidant capacity in serum and ileal of groups C and D was significantly higher than that of the control group (P<0.05). The jejunal glutathione content of group D was significantly higher than that of the control group (P<0.05), and the jejunum total superoxide dismutase activity of groups C and D was significantly higher than that of the control group (P<0.05). The ileal glutathione peroxidase activity of group D was significantly higher than that of the control group (P<0.05). 4) The jejunal villi height/crypt depth (V/C) of group C was significantly higher than that of the control group (P<0.05). The ileal villus height of groups B, C and D was significantly higher than that of the control group (P<0.05), and the ileal V/C of groups C and D was significantly higher than that of the control group (P<0.05). It is concluded that dietary Clostridium butyricum, Bacillus subtilis and their mixture preparation can improve the total weight gain, jejunal lipase activity and antioxidant capacity of meat geese, in addition, dietary Bacillus subtilis can improve the intestinal morphology better.

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