动物营养学报 >

2021 , Vol. 33 >Issue 2: 900 - 912

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.02.031

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

竹叶提取物对热应激奶牛泌乳性能及血清生化、抗氧化和免疫指标的影响

  • 李义 ,
  • 童津津 ,
  • 栗明月 ,
  • 韩思雨 ,
  • 赵璐宇 ,
  • 方洛云 ,
  • 蒋林树
展开
  • 北京农学院动物科学技术学院, 奶牛营养学北京市重点实验室, 北京 102206
李义(1997-),男,山东临沂人,硕士研究生,从事奶牛营养与免疫研究。E-mail:liyi_123_321@163.com

收稿日期: 2020-10-22

  网络出版日期: 2021-02-04

基金资助

北京市农业科技示范推广项目（20200130）；国家"十三五"重点研发计划（2016YFD0700201）；北京农学院学位与研究生教育改革与发展项目（5076516017/026）

收起

Effects of Bamboo Leaf Extract on Lactation Performance and Indexes of Biochemical, Antioxidant and Immune in Serum of Dairy Cows during Heat Stress

  • LI Yi ,
  • TONG Jinjin ,
  • LI Mingyue ,
  • HAN Siyu ,
  • ZHAO Luyu ,
  • FANG Luoyun ,
  • JIANG Linshu
Expand
  • Beijing Key Laboratory for Dairy Cow Nutrition, Beijing University of Agriculture, Beijing 102206, China

Received date: 2020-10-22

  Online published: 2021-02-04

Supported by

 

Fold

摘要

本试验旨在研究竹叶提取物对热应激奶牛泌乳性能及血清生化、抗氧化和免疫指标的影响。试验于2019年7—8月进行，试验期间日平均温湿度指数为75.87，奶牛处于自然热应激状态。选取24头体重[（559.2±37.4） kg]、泌乳天数[（185.7±16.9） d]、胎次[（3.3±0.3）胎]和产奶量[（37.6±0.8） kg/d]相近的荷斯坦奶牛，随机分为2组，每组12头。对照组饲喂全混合日粮（TMR），试验组在TMR基础上添加1.3 g/kg DM的竹叶提取物。预试期14 d，正试期21 d。分别于正试期第1、11、21天测量奶牛呼吸频率、直肠温度并采集血样和奶样，测定泌乳性能及血清生化、抗氧化和免疫指标。结果表明：1）试验组热应激奶牛的呼吸频率极显著低于对照组（P<0.01），直肠温度与对照组相比有降低的趋势（P=0.09）。2）试验组热应激奶牛的产奶量和乳脂率极显著高于对照组（P<0.01），乳中体细胞数与对照组相比有降低的趋势（P=0.09），但干物质采食量、乳蛋白率和乳糖率与对照组无显著差异（P>0.05）。3）试验组热应激奶牛的血清肾上腺素含量极显著高于对照组（P<0.01），血清前列腺素-2含量显著高于对照组（P<0.05），血清游离脂肪酸含量与对照组相比有升高的趋势（P=0.06），但血清糖皮质激素、三碘甲状腺原氨酸、甲状腺素、葡萄糖含量及乳酸脱氢酶活性与对照组无显著差异（P>0.05）。4）试验组热应激奶牛的血清超氧化物歧化酶和谷胱甘肽过氧化氢酶活性极显著高于对照组（P<0.01），血清丙二醛含量极显著低于对照组（P<0.01）。5）试验组热应激奶牛的血清肿瘤坏死因子-α和白细胞介素-2含量极显著高于对照组（P<0.01），血清白细胞介素-1和白细胞介素-1β含量极显著低于对照组（P<0.01），血清免疫球蛋白A含量显著高于对照组（P<0.05），血清免疫球蛋白G含量与对照组相比有升高的趋势（P=0.08），但血清干扰素-γ、白细胞介素-6和免疫球蛋白M含量与对照组无显著差异（P>0.05）。综上所述，饲粮中添加1.3 g/kg DM竹叶提取物降低了热应激奶牛的呼吸频率和直肠温度，提高了泌乳性能，缓解了机体的代谢紊乱，增强了机体的抗氧化能力，改善了机体的免疫功能。

关键词： 竹叶提取物; 奶牛; 热应激; 生化指标; 抗氧化指标; 免疫指标

本文引用格式

李义 , 童津津 , 栗明月 , 韩思雨 , 赵璐宇 , 方洛云 , 蒋林树 . 竹叶提取物对热应激奶牛泌乳性能及血清生化、抗氧化和免疫指标的影响[J]. 动物营养学报, 2021 , 33(2) : 900 -912 . DOI: 10.3969/j.issn.1006-267x.2021.02.031

Abstract

This experiment was conducted to investigate the effects of bamboo leaf extract on lactation performance and indexes of biochemical, antioxidant and immune in serum of dairy cows during heat stress. The experiment was conducted from July to August in 2019, the daily average temperature-humidity index was 75.87 during the experiment, and the dairy cows were in a state of natural heat stress. Twenty-four Holstein cows with similar body weight [(559.2±37.4) kg], days of lactation [(185.7±16.9) d], parity [(3.3±0.3) parities] and milk yield [(37.6±0.8) kg/d] were randomly divided into 2 groups with 12 cows per group. Cows in the control group were fed a total mixed ration (TMR), and others in the experimental group were fed the TMR supplemented with 1.3 g/kg DM bamboo leaf extract. The pre-experimental period lasted for 14 days, and the experimental period lasted for 21 days. On days 1, 11 and 21 of experimental period, the respiratory rate and rectal temperature were measured, and the milk samples and blood samples were collected to determine the lactation performance and indexes of biochemical, antioxidant and immune in serum. The results showed as follows: 1) the respiratory rate of dairy cows during heat stress in the experimental group was significantly lower than that in the control group (P<0.01), and the rectal temperature had a decreasing trend compared with the control group (P=0.09). 2) The milk yield and milk fat percentage of dairy cows during heat stress in the experimental group were significantly higher than those in the control group (P<0.01), the milk somatic cell count had a decreasing trend compared with the control group (P=0.09), but the dry matter intake, milk protein percentage and lactose percentage had no significant differences compared with the control group (P>0.05). 3) The serum adrenaline content of dairy cows during heat stress in the experimental group was significantly higher than that in the control group (P<0.01), the serum prostaglandin-2 content was significantly higher than that in the control group (P<0.05), the serum free fatty acid content had a increasing trend compared with the control group (P=0.06), but the contents of glucocorticoid, triiodothyronine, thyroxine, glucose and activity of lactate dehydrogenase in serum had no significant differences compared with the control group (P>0.05). 4) The activities of superoxide dismutase and glutathione catalase in serum of dairy cows during heat stress in the experimental group were significantly higher than those in the control group (P<0.01), and the serum malondialdehyde content was significantly lower than that in the control group (P<0.01). 5) The contents of tumor necrosis factor-α and interleukin-2 in serum of dairy cows during heat stress in the experimental group were significantly higher than those in the control group (P<0.01), the contents of interleukin-1 and interleukin-1β in serum were significantly lower than those in the control group (P<0.01), the serum immunoglobulin A content was significantly higher than that in the control group (P<0.05), the serum immunoglobulin G content had a increasing trend compared with the control group (P=0.08), but the contents of interferon-γ, interleukin-6 and immunoglobulin M in serum had no significant differences compared with the control group (P>0.05). It is concluded that dietary supplemented with 1.3 g/kg DM bamboo leaf extract can reduce the respiratory rate and rectal temperature of dairy cows during heat stress, improve the lactation performance, alleviate the body metabolic disorders and enhance antioxidant capacity and immune function.

Key words： bamboo leaf extract; dairy cows; heat stress; biochemical indexes; antioxidant indexes; immune indexes

参考文献

[1] YORK L,HEFFERNAN C,RYMER C,et al.A deterministic evaluation of heat stress mitigation and feed cost under climate change within the smallholder dairy sector[J].Animal,2017,11(5):900-909.  
[2] GERNAND E,KONIG S,KIPP C.Influence of on-farm measurements for heat stress indicators on dairy cow productivity, female fertility and health[J].Journal of Dairy Science,2019,102(7):6660-6671.  
[3] PERDOMO M C,MARSOLA R S,FAVORETO M G,et al.Effects of feeding live yeast at 2 dosages on performance and feeding behavior of dairy cows under heat stress[J].Journal of Dairy Science,2020,103(1):325-339.  
[4] SAMMAD A,UMER S,SHI R,et al.Dairy cow reproduction under the influence of heat stress[J].Journal of Animal Physiology and Animal Nutrition,2020,104(4):978-986.  
[5] CHEN K L,WANG H L,JIANG L Z,et al.Heat stress induces apoptosis through disruption of dynamic mitochondrial networks in dairy cow mammary epithelial cells[J].In Vitro Cellular and Developmental Biology Animal,2020,56(4):322-331.  
[6] LEE J S,KANG S,KIM M J,et al.Dietary supplementation with combined extracts from garlic (Allium sativum),brown seaweed (Undaria pinnatifida),and pinecone (Pinus koraiensis) improves milk production in Holstein cows under heat stress conditions[J].Asian-Australasian Journal of Animal Sciences,2020,33(1):111-119.  
[7] QING G,HANGQING L,PENGTAO W,et al.Investigation of physicochemical properties and antioxidant activity of ultrafine bamboo leaf powder prepared by ball milling[J].Journal of Food Processing and Preservation,2020,44(7):175-184.
[8] WANG J,TANG F,YUE Y,et al.Development and validation of an HPTLC method for simultaneous quantitation of isoorientin,isovitexin, orientin,and vitexin in bamboo-leaf flavonoids[J].Journal of AOAC International,2010,93(5):1376-1383.  
[9] 张金枝,邵庆均,吴晓琴,等.竹叶黄酮对断奶仔猪生产性能的影响[J].中国畜牧杂志,2003(1):25-29. ZHANG J Z,SHAO Q J,WU X Q,et al.Effect of bamboo leaf flavone on the performance of weaned piglets[J].Chinese Journal of Animal Science,2003(1):25-29.(in Chinese)
[10] 杨奇,杨宇生,曾妮,等.竹叶黄酮对持续高温状态下肉鸡生长性能和屠宰性能的影响[J].饲料工业,2014,35(8):6-8. YANG Q,YANG Y S,ZENG N,et al.Effects of bamboo flavones on growth performance and slaughtering performance of broiler chickens under continuous high temperature[J].Feed Industry Magazine,2014,35(8):6-8.(in Chinese)
[11] 侯昆,童津津,楚康康,等.竹叶黄酮与青蒿提取物对患隐性乳房炎奶牛产奶性能、乳中体细胞数及血清免疫和抗氧化相关指标的影响[J].动物营养学报,2019,31(9):4286-4295. HOU K,TONG J J,CHU K K,et al.Effects of bamboo leaf flavonoids and artemisia annua extract on milk performance,milk somatic cell count and serum immune and antioxidant related indexes of dairy cows with subclinical mastitis[J].Chinese Journal of Animal Nutrition,2019,31(9):4286-4295.(in Chinese)
[12] 栗明月,方洛云,苏汉书,等.竹叶提取物对奶牛泌乳性能、血液常规指标、免疫和抗氧化性能的影响[J].动物营养学报,2019,31(7):3302-3309. LI M Y,FANG L Y,SUN H S,et al.Effects of bamboo leaf extracts on lactation performance,blood routine indexes and immune and antioxidant ability of dairy cows[J].Chinese Journal of Animal Nutrition,2019,31(7):3302-3309.(in Chinese)
[13] AOAC.Official methods of analysis[S].15th ed.Arlington VA:AOAC International Press,2011.
[14] SONG X,LUO J,FU D,et al.Traditional Chinese medicine prescriptions enhance growth performance of heat stressed beef cattle by relieving heat stress responses and increasing apparent nutrient digestibility[J].Asian-Australasian Journal of Animal Sciences,2014,27(10):1513-1520.  
[15] OTTO P I,GUIMARAES S,VERARDO L L,et al.Genome-wide association studies for heat stress response in Bos taurus×Bos indicus crossbred cattle[J].Journal of Dairy Science,2019,102(9):8148-8158.  
[16] 刘德义,贺绍君,刘世清,等.大豆黄酮对热应激奶牛血细胞数和生理常数的影响[J].中国奶牛,2013(1):23-26. LIU D Y,HE S J,LIU S Q,et al.Effects of soy flavones on blood cell count and physiological constants in cows under heat stress[J].China Dairy Cattle,2013(1):23-26.(in Chinese)
[17] ZHANG L,MAO Y,PAN J,et al.Bamboo leaf extract ameliorates cardiac fibrosis possibly via alleviating inflammation, oxidative stress and apoptosis[J].Biomedicine & Pharmacotherapy,2017,95(21):808-817.
[18] XUN Q,HU C,LI X,et al.GLCCI1 rs37973 is associated with the response of adrenal hormone to inhaled corticosteroids in asthma[J].World Allergy Organization Journal,2019,12(3):100017.
[19] SAFA S,KARGAR S,MOGHADDAM G A,et al.Heat stress abatement during the postpartum period:effects on whole lactation milk yield,indicators of metabolic status,inflammatory cytokines,and biomarkers of the oxidative stress[J].Journal of Animal Science,2019,97(1):122-132.  
[20] ZHAN J,LIU M,SU X,et al.Effects of alfalfa flavonoids on the production performance,immune system,and ruminal fermentation of dairy cows[J].Asian-Australasian Journal of Animal Sciences,2017,30(10):1416-1424.  
[21] GESSNER D K,KOCH C,ROMBERG F J,et al.The effect of grape seed and grape marc meal extract on milk performance and the expression of genes of endoplasmic reticulum stress and inflammation in the liver of dairy cows in early lactation[J].Journal of Dairy Science,2015,98(12):8856-8868.  
[22] GJERSTAD J K,LIGHTMAN S L,SPIGA F.Role of glucocorticoid negative feedback in the regulation of HPA axis pulsatility[J].Stress-The International Journal on The Biology of Stress,2018,21(5):403-416.  
[23] SCHWINN A C,SAUER F J,GERBER V,et al.Free and bound cortisol in plasma and saliva during ACTH challenge in dairy cows and horses[J].Journal of Animal Science,2018,96(1):76-84.  
[24] 刘嘉莉,窦金焕,胡丽蓉,等.热应激对奶牛生理和免疫功能的影响及其机理[J].中国畜牧兽医,2018,45(1):263-270. LIU J L,DOU J H,HU L R,et al.Effects of Heat stress on physiological and immune function of dairy cows and its mechanism[J].China Animal Husbandry and Veterinary Medicine,2018,45(1):263-270.(in Chinese)
[25] MARETT L C,AULDIST M J,WALES W J,et al.Responses to metabolic challenges in dairy cows with high or low milk yield during an extended lactation[J].Journal of Dairy Science,2019,102(5):4590-4605.  
[26] LEITE D S W,POEHLAND R,CARVALHO D O C,et al.Shading effect on physiological parameters and in vitro embryo production of tropical adapted Nellore heifers in integrated crop-livestock-forest systems[J].Tropical Animal Health and Production,2020,52(5):2273-2281.  
[27] LH B,JB W,SR S,et al.Postabsorptive carbohydrate adaptations to heat stress and monensin supplementation in lactating Holstein cows[J].Journal of Dairy Science,2011,94(11):5620-5633.  
[28] 宋小珍,付戴波,瞿明仁,等.热应激对肉牛血清内分泌激素含量、抗氧化酶活性及生理生化指标的影响[J].动物营养学报,2012,24(12):2485-2490. SONG X Z,FU D B,QU M R,et al.Effects of heat stress on serum endocrine hormone content,antioxidant enzyme activity and physiological and biochemical indexes of beef cattle[J].Chinese Journal of Animal Nutrition,2012,24(12):2485-2490.(in Chinese)
[29] COLLIER R J,STIENING C M,POLLARD B C,et al.Use of gene expression microarrays for evaluating environmental stress tolerance at the cellular level in cattle[J].Journal of Animal Science,2006,84(7):123-135.
[30] AMER H A,AWAD E T,EL-BATRAWI A M.Effect of aflatoxin B1 on alkaline phosphatase and lactic dehydrogenase levels in liver and serum of broiler chicks[J].Archiv fur Experimentelle Veterinarmedizin,1988,42(4):595-602.
[31] REICHMANN D,VOTH W,JAKOB U.Maintaining a healthy proteome during oxidative stress[J].Molecular Cell,2018,69(2):203-213.  
[32] SURAI P F,KOCHISH I I,FISININ V I,et al.Revisiting oxidative stress and the use of organic selenium in dairy cow nutrition[J].Animals,2019,9(7):104-114.
[33] GONG J,XIA D,HUANG J,et al.Functional components of bamboo shavings and bamboo leaf extracts and their antioxidant activities in vitro[J].Journal of Medicinal Food,2015,18(4):453-459.  
[34] YU Y,LI Z,CAO G,et al.Bamboo leaf flavonoids extracts alleviate oxidative stress in Hepg2 cells via naturally modulating reactive oxygen species production and Nrf2-MEdiated antioxidant defense responses[J].Journal of Food Science,2019,84(6):1609-1620.  
[35] LU L L,SMITH M T,YU K,et al.IFN-gamma-independent immune markers of Mycobacterium tuberculosis exposure[J].Nature Medicine,2019,25(6):977-987.  
[36] BALDASSARRE M,NALDI M,DOMENICALI M,et al.Simple and rapid LC-MS method for the determination of circulating albumin microheterogeneity in veal calves exposed to heat stress[J].Journal of Pharmaceutical and Biomedical Analysis,2017,144(11):263-268.
[37] SHAN C H,GUO J,SUN X,et al.Effects of fermented Chinese herbal medicines on milk performance and immune function in late-lactation cows under heat stress conditions[J].Journal of Animal Science,2018,96(10):4444-4457.
[38] MA F T,SHAN Q,JIN Y H,et al.Effect of Lonicera japonica extract on lactation performance,antioxidant status,and endocrine and immune function in heat-stressed mid-lactation dairy cows[J].Journal of Dairy Science,2020(5):417-428.
[39] 李留安,杨晓静,杜改梅,等.HPA轴应激应答反应产生的中枢调节机制[J].中国兽医杂志,2010(6):65-66. LI L A,YANG X J,DU G M,et al.The central regulatory mechanism of HPA axis stress response[J].Chinese Journal of Veterinary Medicine,2010(6):65-66.(in Chinese)
[40] STRONG R A,SILVA E B,CHENG H W,et al.Acute brief heat stress in late gestation alters neonatal calf innate immune functions[J].Journal of Dairy Science,2015,98(11):7771-7783.  
[41] YAGHOUBI S M,GHORBANI G R,RAHMANI H R,et al.Growth, weaning performance and blood indicators of humoral immunity in Holstein calves fed supplemental flavonoids[J].Journal of Animal Physiology and Animal Nutrition,2008,92(4):456-462.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2