水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

不同来源虾青素对虹鳟生长性能、肉色和抗氧化能力的影响

  • 张春燕 ,
  • 文登鑫 ,
  • 姚文祥 ,
  • 李小勤 ,
  • 吴世林 ,
  • 冷向军
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  • 1. 上海海洋大学水产与生命学院, 水产科学国家级教学示范中心, 上海 201306;
    2. 上海海洋大学水产与生命学院, 农业部鱼类营养与环境生态研究中心, 上海 201306;
    3. 上海海洋大学水产与生命学院, 水产动物遗传育种中心上海市协同创新中心, 上海 201306;
    4. 广州智特奇生物科技有限公司, 广州 510663
张春燕(1996-),女,浙江平湖人,硕士研究生,研究方向为水产动物营养。E-mail:931780463@qq.com

收稿日期: 2020-07-16

  网络出版日期: 2021-02-04

基金资助

国家自然科学基金(31772858)

Effects of Different Astaxanthin Sources on Growth Performance, Flesh Color and Antioxidant Ability of Rainbow Trout (Oncorhynchus mykiss)

  • ZHANG Chunyan ,
  • WEN Dengxin ,
  • YAO Wenxiang ,
  • LI Xiaoqin ,
  • WU Shilin ,
  • LENG Xiangjun
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  • 1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;
    2. Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;
    3. Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China;
    4. Guangzhou Wisdom Bio-Technology Co., Ltd., Science Avenue, Guangzhou 510663, China

Received date: 2020-07-16

  Online published: 2021-02-04

Supported by

 

摘要

本试验旨在研究不同来源虾青素对虹鳟生长性能、肉色和抗氧化能力的影响。选取体质健壮、大小均匀的虹鳟375尾[平均体重为(6.28±0.07) g],随机分为5组,每组3个重复,每个重复25尾。试验配制5种等氮等能的饲料,分别为基础饲料和在基础饲料(对照组)中分别添加1.0 g/kg合成虾青素(Ast)、6.5 g/kg福寿花花瓣粉(AF)、3.4 g/kg福寿花提取物(AE)和4.4 g/kg雨生红球藻提取物(HE)(折算成虾青素含量均为100 mg/kg)的试验饲料,喂养虹鳟6周。结果表明:饲料中添加Ast、AE和HE对虹鳟增重率和饲料系数无显著影响(P>0.05),但AF组的增重率较对照组显著降低(P<0.05),饲料系数显著提高(P<0.05)。Ast、AF、AE和HE组的肌肉红度和黄度值、组织虾青素含量和血清类胡萝卜素含量(除AF组第2周)均显著高于对照组(P<0.05),肌肉亮度值显著低于对照组(P<0.05)。与对照组相比,饲料中添加Ast、AE和HE显著降低了肌肉冷冻损失和4、6 h的滴水损失,肌肉、肝脏和血清总超氧化物歧化酶活性和丙二醛含量(P<0.05),显著提高了抑制羟自由基能力(P<0.05)。上述结果表明,饲料中添加AE、HE能有效改善虹鳟肌肉颜色,增强机体抗氧化能力,达到和添加Ast一致的效果,但AF不宜直接用作虹鳟的着色剂。

本文引用格式

张春燕 , 文登鑫 , 姚文祥 , 李小勤 , 吴世林 , 冷向军 . 不同来源虾青素对虹鳟生长性能、肉色和抗氧化能力的影响[J]. 动物营养学报, 2021 , 33(2) : 1008 -1019 . DOI: 10.3969/j.issn.1006-267x.2021.02.041

Abstract

This study was investigated that the effects of different astaxanthin sources on the growth performance, flesh color and antioxidant ability of rainbow trout (Oncorhynchus mykiss). Three hundred and seventy-five rainbow trout with an average body weight of (6.27±0.04) g were randomly assigned to 5 groups with 3 replicates per group and 25 fish per replicate. Five iso-nitrogenous and iso-energetic diets were designed as the control diet and the astaxanthin sources supplemented diets, including 1.0 g/kg synthetic astaxanthin product (Ast), 6.5 g/kg Adonis aestivalis flower (AF), 3.4 g/kg Adonis aestivalis extract (AE) and 4.4 g/kg Haematococcus pluvialis extract (HE) with the same astaxanthin inclusion of 100 mg/kg. Then the five diets were fed to rainbow trout for 6 weeks. The results showed as follows: adding Ast, AE and HE had no significant effects on weight gain rate (WGR) and feed conversion ratio (FCR) (P>0.05). However, weight gain rate (WGR) in AF group significantly decreased (P<0.05) and feed conversion rate (FCR) significantly increased (P<0.05). The flesh redness (a*), yellowness (b*) values, tissue astaxanthin content and the serum carotenoid content (except the AF group at the 2nd week) in Ast, AF, AE and HE groups were significantly higher than those in control group (P<0.05), and the flesh lightness (L*) value was significantly lower than that in the control group (P<0.05). Compared with the control group, the supplementation of Ast, AE and HE significantly decreased the flesh thawing loss, drip loss at the 4 and 6 h (P<0.05), significantly reduced the total superoxide dismutase activity and malondialdehyde content in flesh, liver and serum (P<0.05), and significantly increased inhibition ability of hydroxyl radical (P<0.05). In conclusion, the supplementation of AE and HE in diet effectively improve the flesh pigmentation and antioxidant capacity of rainbow trout, and reach the similar effects to synthetic astaxanthin, while AF is not suggested to be used in the diet of rainbow trout.

参考文献

[1] BAKER R T M,PFEIFFER A M,SCHÖNER F J,et al.Pigmenting efficacy of astaxanthin and canthaxanthin in fresh-water reared Atlantic salmon,Salmo salar[J].Animal Feed Science and Technology,2002,99(1/2/3/4):97-106.
[2] PHAM M A,BYUN H G,KIM K D,et al.Effects of dietary carotenoid source and level on growth,skin pigmentation,antioxidant activity and chemical composition of juvenile olive flounder Paralichthys olivaceus[J].Aquaculture,2014,431:65-72.
[3] STOREBAKKEN T,SØRENSEN M,BJERKENG B,et al.Utilization of astaxanthin from red yeast,Xanthophyllomyces dendrorhous,in rainbow trout,Oncorhynchus mykiss:effects of enzymatic cell wall disruption and feed extrusion temperature[J].Aquaculture,2004,236(1/2/3/4):391-403.
[4] TEIMOURI M,AMIRKOLAIE A K,YEGANEH S.The effects of Spirulina platensis meal as a feed supplement on growth performance and pigmentation of rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,2013,396:14-19.
[5] YUAN J P,CHEN F,LIU X,et al.Carotenoid composition in the green microalga Chlorococcum[J].Food Chemistry,2002,76(3):319-325.  
[6] RENSTRØM B,BERGER H,LIAAEN-JENSEN S.Esterified,optically pure (3S,3'S)-astaxanthin from flowers of Adonis annua[J].Biochemical Systematics and Ecology,1981,9(4):249-250.  
[7] HOSSEINI M,TAHERKHANI M,GHORBANI NOHOOJI M.Introduction of Adonis aestivalis as a new source of effective cytotoxic cardiac glycoside[J].Natural Product Research,2019,33(6):915-920.  
[8] KAMATA T,NEAMTU G,TANAKA Y,et al.Utilization of Adonis aestivalis as a dietary pigment source for rainbow trout Salmo gairdneri[J].Nippon Suisan Gakkaishi,1990,56(5):783-788.  
[9] LORENZ R T,CYSEWSKI G R.Commercial potential for Haematococcus microalgae as a natural source of astaxanthin[J].Trends in Biotechnology,2000,18(4):160-167.  
[10] AL-BULISHI M S M,XUE C H,TANG Q J.Health aspects of astaxanthin:a review[J].Canadian Journal of Clinical Nutrition,2015,3(2):71-78.  
[11] HAGEN C,SIEGMUND S,BRAUNE W.Ultrastructural and chemical changes in the cell wall of Haematococcus pluvialis (Volvocales,Chlorophyta) during aplanospore formation[J].European Journal of Phycology,2002,37(2):217-226.  
[12] SHEIKHZADEH N,TAYEFI-NASRABADI H,OUSHANI A K,et al.Effects of Haematococcus pluvialis supplementation on antioxidant system and metabolism in rainbow trout (Oncorhynchus mykiss)[J].Fish Physiology and Biochemistry,2012,38(2):413-419.  
[13] TOLASA S,CAKLI S,OSTERMEYER U.Determination of astaxanthin and canthaxanthin in salmonid[J].European Food Research and Technology,2005,221(6):787-791.  
[14] TEJERA N,CEJAS J R,RODRIGUEZ C,et al.Pigmentation,carotenoids,lipid peroxides and lipid composition of skin of red porgy (Pagrus pagrus) fed diets supplemented with different astaxanthin sources[J].Aquaculture,2007,270(1/2/3/4):218-230.
[15] LI M,WU W J,ZHOU P P,et al.Comparison effect of dietary astaxanthin and Haematococcus pluvialis on growth performance,antioxidant status and immune response of large yellow croaker Pseudosciaena crocea[J].Aquaculture,2014,434:227-232.
[16] ZAŤKOVÁ I,SERGEJEVOVÁ M,URBAN J,et al.Carotenoid-enriched microalgal biomass as feed supplement for freshwater ornamentals:albinic form of wels catfish (Silurus glanis)[J].Aquaculture Nutrition,2011,17(3):278-286.  
[17] JU Z Y,DENG D F,DOMINY W G,et al.Pigmentation of Pacific White shrimp,Litopenaeus vannamei,by dietary astaxanthin extracted from Haematococcus pluvialis[J].Journal of the World Aquaculture Society,2011,42(5):633-644.  
[18] ZHAO X X,HU J,ZHANG X Q,et al.Effects of E/Z isomers and coating materials of astaxanthin products on the pigmentation and antioxidation of rainbow trout,Oncorhynchus mykiss[J].Journal of the World Aquaculture Society,2016,47(3):341-351.  
[19] ZHANG J J,LI X Q,LENG X J,et al.Effects of dietary astaxanthins on pigmentation of flesh and tissue antioxidation of rainbow trout (Oncorhynchus mykiss)[J].Aquaculture International,2013,21(3):579-589.  
[20] SONG X L,WANG L,LI X Q,et al.Dietary astaxanthin improved the body pigmentation and antioxidant function,but not the growth of discus fish (Symphysodon spp.)[J].Aquaculture Research,2017,48(4):1359-1367.  
[21] CHRISTIANSEN R,TORRISSEN O J.Growth and survival of Atlantic salmon,Salmo salar L.fed different dietary levels of astaxanthin.Juveniles[J].Aquaculture Nutrition,1996,2(1):55-62.  
[22] 王磊,陈再忠,冷向军,等.饲料中添加雨生红球藻对七彩神仙鱼生长、体色及抗氧化力的影响[J].淡水渔业,2016,46(6):92-97. WANG L,CHEN Z Z,LENG X J,et al.Effect of Haematococcus pluvialis on growth,body color andantioxidation capacity of discus fish Symphysodon haraldi[J].Freshwater Fisheries,2016,46(6):92-97.(in Chinese)
[23] PAGE G I,DAVIES S J.Tissue astaxanthin and canthaxanthin distribution in rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar)[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2006,143(1):125-132.  
[24] YANAR Y,BÜYÜKÇAPAR H,YANAR M,et al.Effect of carotenoids from red pepper and marigold flower on pigmentation,sensory properties and fatty acid composition of rainbow trout[J].Food Chemistry,2007,100(1):326-330.  
[25] AMAR E C,KIRON V,SATOH S,et al.Influence of various dietary synthetic carotenoids on bio-defence mechanisms in rainbow trout,Oncorhynchus mykiss (Walbaum)[J].Aquaculture Research,2001,32(Suppl.1):162-173.
[26] RAHMAN M M,KHOSRAVI S,CHANG K H,et al.Effects of dietary inclusion of astaxanthin on growth,muscle pigmentation and antioxidant capacity of juvenile rainbow trout (Oncorhynchus mykiss)[J].Preventive Nutrition and Food Science,2016,21(3):281-288.  
[27] DE LA MORA G I,ARREDONDO-FIGUEROA J L,PONCE-PALAFOX J T,et al.Comparison of red chilli (Capsicum annuum) oleoresin and astaxanthin on rainbow trout (Oncorhyncus mykiss) fillet pigmentation[J].Aquaculture,2006,258(1/2/3/4):487-495.
[28] KAMATA T,TANAKA Y,YAMADA S,et al.Study of carotenoid composition and fatty acids of astaxanthin diester in rainbow trout Salmo gairdneri fed the Adonis extract[J].Nippon Suisan Gakkaishi,1990,56(5):789-794.  
[29] MAOKA T,ETOH T,KISHIMOTO S,et al.Carotenoids and their fatty acid esters in the petals of Adonis aestivalis[J].Journal of Oleo Science,2011,60(2):47-52.  
[30] SOMMER T R,POTTS W T,MORRISSY N M.Utilization of microalgal astaxanthin by rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,1991,94(1):79-88.  
[31] HORN D,RIEGER J.Organic nanoparticles in the aqueous phase-theory,experiment,and use[J].Angewandte Chemie (International Edition),2001,40(23):4330-4361.  
[32] HENMI H,IWATA T,HATA M.Studies on the carotenoids in the muscle of salmons Ⅰ.Intracellular distribution of carotenoids in the muscle studies on the carotenoids in the muscle of salmons[J].Tohoku Journal of Agricultural Research,1987,37(3/4):101-111.
[33] BOWEN J,SOUTAR C,SERWATA R D,et al.Utilization of (3S,3'S)-astaxanthin acyl esters in pigmentation of rainbow trout (Oncorhynchus mykiss)[J].Aquaculture Nutrition,2002,8(1):59-68.  
[34] SCHIEDT K.Absorption and metabolism of carotenoids in birds,fish and crustaceans[M]//BRITTON G,LIAAEN-JENSEN S,PFANDER H.Carotenoids volume 3:biosynthesis and metabolism.Basel,Switzerland:Birkhäuser,1998:285-358.
[35] 周庆新,杨鲁,徐杰.雨生红球藻源虾青素酯的消化吸收特性研究[J].中国食品学报,2019,19(4):125-132. ZHOU Q X,YANG L,XU J,et al.Studies on the digestion and absorption characteristics of esterified astaxanthins from Haematococcus pluvialis[J].Journal of Chinese Institute of Food Science and Technology,2019,19(4):125-132.(in Chinese)
[36] 苏芳.类胡萝卜素在藻虾蟹鱼中的结构分布特征及虾青素异构化研究[D].博士学位论文.青岛:中国科学院海洋研究所,2018:93-99. SU F.A study on the structure distribution of carotenoids in algae,shrimps,crabs and fishes and the isomerization of astaxanthin[D].Ph.D.Thesis.Qingdao:Institute of Oceanology,Chinese Academy of Sciences,2018:93-99.(in Chinese)
[37] NAKANO T,KANMURI T,SATO M,et al.Effect of astaxanthin rich red yeast (Phaffia rhodozyma) on oxidative stress in rainbow trout[J].Biochimica et Biophysica Acta:General Subjects,1999,1426(1):119-125.  
[38] PAN C H,CHIEN Y H,WANG Y J,et al.Antioxidant defence to ammonia stress of characins (Hyphessobrycon eques Steindachner) fed diets supplemented with carotenoids[J].Aquaculture Nutrition,2011,17(3):258-266.  
[39] WANG Y J,CHIEN Y W,PAN C H,et al.Effects of dietary supplementation of carotenoids on survival,growth,pigmentation,and antioxidant capacity of characins,Hyphessobrycon callistus[J].Aquaculture,2006,261(2):641-648.  
[40] PAN C H,CHIEN Y H,HUNTER B.The resistance to ammonia stress of Penaeus monodon Fabricius juvenile fed diets supplemented with astaxanthin[J].Journal of Experimental Marine Biology and Ecology,2003,297(1):107-118.  
[41] NAGUIB Y M A.Antioxidant activities of astaxanthin and related carotenoids[J].Journal of Agricultural and Food Chemistry,2000,48(4):1150-1154.  
[42] 李小勤,胡斌,冷向军,等.VE对草鱼成鱼肌肉品质和抗氧化性能的影响[J].水生生物学报,2009,33(6):1132-1139. LI X Q,HU B,LENG X J,et al.Effect of supplemental VE on meat quality and antioxidant capacity of adult grass carp[J].Acta Hydrobiologica Sinica,2009,33(6):1132-1139.(in Chinese)
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