分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

高能饲粮对育成期蛋鸡肝脏miRNA表达谱的影响

  • 王星果 ,
  • 卢建 ,
  • 关树勇 ,
  • 曲亮 ,
  • 孙平江 ,
  • 郭军 ,
  • 胡玉萍 ,
  • 窦套存 ,
  • 马猛 ,
  • 李永峰 ,
  • 王克华
展开
  • 1. 江苏省家禽科学研究所, 扬州 225125;
    2. 南京农业大学, 南京 210095;
    3. 江苏峪口禽业有限公司, 宿迁 223733
王星果(1984-),男,江苏扬州人,副研究员,博士,主要从事蛋鸡遗传育种和脂质代谢研究。E-mail:yzwangxingguo@163.com

收稿日期: 2020-07-16

  网络出版日期: 2021-02-04

基金资助

江苏省家禽遗传育种重点实验室资助项目(JQLAB-ZZ-202003);省属公益类科研院所自主科研经费(BM2018026);现代农业产业技术体系建设专项资金(CARS-40-K01);江苏省农业重大新品种创制项目(PZCZ201729);江苏省自然科学基金青年基金(BK20190240)

Effects of High Energy Diet on Liver miRNA Expression Profile of Laying Hens during Growing Period

  • WANG Xingguo ,
  • LU Jian ,
  • GUAN Shuyong ,
  • QU Liang ,
  • SUN Pingjiang ,
  • GUO Jun ,
  • HU Yuping ,
  • DOU Taocun ,
  • MA Meng ,
  • LI Yongfeng ,
  • WANG Kehua
Expand
  • 1. Jiangsu Institute of Poultry Science, Yangzhou 225125, China;
    2. Nanjing Agricultural University, Nanjing 210095, China;
    3. Jiangsu Yukou Poultry Industry Co., Ltd., Suqian 223733, China

Received date: 2020-07-16

  Online published: 2021-02-04

Supported by

 

摘要

本研究利用高能和低能饲粮饲喂育成期蛋鸡,运用small RNA测序方法对肝脏中miRNA进行检测,对其表达量进行分析,对差异表达miRNA进行靶基因预测,并对差异表达的预测靶基因进行基因本体论(GO)功能和京都基因与基因组百科全书(KEGG)信号通路富集分析。结果表明:与低能组相比,高能组有2个miRNA表达显著上调(P<0.05),6个miRNA表达显著下调(P<0.05);在差异表达基因中共预测出差异表达miRNA的41个靶基因,其中miR-15a的预测靶基因最多,为26个;差异表达靶基因GO和KEGG分析发现,免疫和分子功能调控是富集程度最高的过程,而显著富集的信号通路与免疫和脂质代谢相关。由此可见,能量摄入量通过影响miRNA的表达进而上调或下调肝脏中免疫和脂质代谢相关靶基因的表达,最终调控蛋鸡的免疫和脂质代谢,并影响生产性能。

本文引用格式

王星果 , 卢建 , 关树勇 , 曲亮 , 孙平江 , 郭军 , 胡玉萍 , 窦套存 , 马猛 , 李永峰 , 王克华 . 高能饲粮对育成期蛋鸡肝脏miRNA表达谱的影响[J]. 动物营养学报, 2021 , 33(2) : 1081 -1090 . DOI: 10.3969/j.issn.1006-267x.2021.02.048

Abstract

In current study, miRNAs in liver were detected by small RNA sequencing of laying hens fed with high-energy or low-energy during the growing period, and their expression levels were analyzed. Target genes of differentially expressed miRNAs were predicted, and differentially expressed target genes were used to undergo gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis. The results showed that compared with low energy group, the expression levels of 2 miRNAs in high energy group were up-regulated and 6 miRNAs were down-regulated (P<0.05). Forty target genes of differentially expressed miRNAs were predicted in differentially expressed genes, and the number of predicted target genes of miR-15a was the biggest for 26. Analysis of GO and KEGG of differentially expressed target genes showed that immune and regulation of molecular function were the most enriched processes, and the significantly enriched signaling pathways were related with immune and lipid metabolism. It is indicates that energy intake regulates immune and lipid metabolism of laying hens by influencing expression of miRNAs and then upregulates or downregulates expression of immune and lipid metabolism related target genes in liver, and finally affects production performance.

参考文献

[1] 李飞翔,王文建,卞红星,等.蛋鸡饲养管理及营养方面存在的误区[J].中国禽业导刊,2017,34(13):65-67. LI F X,WANG W J,BIAN H X,et al.Mistakes in breeding management and nutrition of laying hens[J].Guide to Chinese Poultry,2017,34(13):65-67.(in Chinese)
[2] 费强.蛋鸡产蛋性能下降的原因及对策[J].家禽科学,2013(2):32-33. FEI Q.Reasons and strategies of performance decrease of laying hens[J].Poultry Science,2013(2):32-33.(in Chinese)
[3] SCHUTTE J B,VAN WEERDEN E J.Requirement of the hen for sulphur-containing amino acids[J].British Poultry Science,1978,19(5):573-581.  
[4] 赵振福,曲长海.维生素在蛋鸡生产中的应用[J].养殖技术顾问,2009(4):42. ZHAO Z F,QU C H.Application of vitamins in production of laying hens[J].Technical Advisor for Animal Husbandry,2009(4):42.(in Chinese)
[5] 张士辉.从饲料配制着手提高蛋鸡产蛋率[J].今日畜牧兽医,2011(5):43-44. ZHANG S H.From feed preparation to improve egg production rate of laying hens[J].Today Animal Husbandry and Veterinary Medicine,2011(5):43-44.(in Chinese)
[6] 呙于明.家禽营养与饲料[M].北京:中国农业大学出版社,1997. GUO Y M.Poultry nutrition and feed[M].Beijing:China Agricultural University Press,1997.(in Chinese)
[7] 张利敏,姚军虎,董延.产蛋鸡粗蛋白质与代谢能需要量研究进展与应用[J].饲料工业,2012,33(3):13-16. ZHANG L M,YAO J H,DONG Y.Research progress and application on crude protein and metabolic energy requirement in laying hens[J].Feed Industry,2012,33(3):13-16.(in Chinese)
[8] ESAU C,DAVIS S,MURRAY S F,et al.miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting[J].Cell Metabolism,2006,3(2):87-98.  
[9] VINCIGUERRA M,SGROI A,VEYRAT-DUREBEX C,et al.Unsaturated fatty acids inhibit the expression of tumor suppressor phosphatase and tensin homolog (PTEN) via microRNA-21 up-regulation in hepatocytes[J].Hepatology,2009,49(4):1176-1184.  
[10] TAKAHASHI K,ODA Y,TOYODA Y,et al.Regulation of cytochrome b5 expression by miR-223 in human liver:effects on cytochrome P450 activities[J].Pharmaceutical Research,2014,31(3):780-794.  
[11] 吕秋凤,董公麟,邱波宁,等.不同能量水平日粮对育成蛋鸡生长性能与胫骨长的影响[C]//全国动物生理生化第七届全国代表大会暨第十三次学术交流会论文集.沈阳:中国畜牧兽医学会,2014. LYU Q F,DONG G L,QIU B N,et al.Effects of diets with different energy levels on growth performance and tibial length of laying hens during growing period[C]//Proceedings of the 7th National Congress and the 13th Academic Meeting of Animal Physiology and Biochemistry.Shenyang:Chinese Association of Animal Science and Veterinary Medicine,2014.(in Chinese)
[12] 张李俊,魏清宇,叶红心,等.育成期白壳蛋鸡不同能量饲料的试验[J].养禽与禽病防治,2005(11):5-6. ZHANG L J,WEI Q Y,YE H X,et al.Experiment on white layer fed by different energy feed during growing period[J].Poultry Husbandry and Disease Control,2005(11):5-6.(in Chinese)
[13] 李娜,徐廷生,雷雪芹,等.卢氏绿壳蛋鸡育成期适宜能量水平的研究[J].中国农学通报,2019,35(5):154-159. LI N,XU T S,LEI X Q,et al.Appropriate energy level for Lushi green-shell egg laying pullets during growing period[J].Chinese Agricultural Science Bulletin,2019,35(5):154-159.(in Chinese)
[14] WANG X G,SHAO F,YU J F,et al.MicroRNA-122 targets genes related to liver metabolism in chickens[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2015,184:29-35.
[15] WANG X G,YU J F,SHAO F,et al.microRNA-122 targets the P4HA1 mRNA and regulates its expression in chicken hepatocytes[J].Italian Journal of Animal Science,2019,18(1):587-593.  
[16] LI Y Y,WANG X G,YU J F,et al.miR-122 targets the vanin 1 gene to regulate its expression in chickens[J].Poultry Science,2016,95(5):1145-1150.  
[17] SHAO F,WANG X G,YU J F,et al.Expression of miR-33 from an SREBF2 intron targets the FTO gene in the chicken[J].PLoS One,2014,9(3):e91236.
[18] SHAO F,WANG X,YU J,et al.Expression of miR-33 from an SREBP2 intron inhibits the expression of the fatty acid oxidation-regulatory genes CROT and HADHB in chicken liver[J].British Poultry Science,2019,60(2):115-124.  
[19] 韩威,盛中伟,朱云芬,等.鸡性成熟启动前后下丘脑microRNA表达谱分析[J].畜牧兽医学报,2017,48(1):23-30. HAN W,SHENG Z W,ZHU Y F,et al. Analysis of hypothalamic microRNA expression profile during chicken puberty onset[J].Chinese Journal of Animal and Veterinary Sciences,2017,48(1):23-30.(in Chinese)
[20] 王免免,周艳婷,张蒙,等.外源皮质酮对雏鸡法氏囊microRNA表达的影响[J].畜牧与兽医,2018,50(5):71-76. WANG M M,ZHOU Y T,ZHANG M,et al.Efect of exogenous corticosterone on the expression of miRNAs in bursa of Fabricius in chicken[J].Animal Husbandry& Veterinary Medicin,2018,50(5):71-76.(in Chinese)
[21] KHATRI B,SEO D,SHOUSE S,et al.MicroRNA profiling associated with muscle growth in modern broilers compared to an unselected chicken breed[J].BMC Genomics,2018,19:683.
[22] 孙瑞萍,王峰,晁哲,等.五指山猪与长白猪骨骼肌miRNA转录组比较分析[J].生物技术通报,2020:1-8.(2020-05-13).https://kns.cnki.net/KCMS/detail/11.2396.Q. 20200513.1438.009.html. SUN R P,WANG F,CHAO Z,et al.Comparative analysis on miRNA transcriptomes of skeletal muscle between Wuzhishan pig and Landrace[J].Biotechnology Bulletin,2020:1-8.(2020-05-13).https://kns.cnki.net/KCMS/detail/11.2396.Q. 20200513.1438.009.html.(in Chinese)
[23] WANG X G,YANG L,WANG H J,et al.Growth hormone-regulated mRNAs and miRNAs in chicken hepatocytes[J].PLoS One,2014,9(11):e112896.
[24] YU N,YONG S,KIM H K,et al.Identification of tumor suppressor miRNAs by integrative miRNA and mRNA sequencing of matched tumor-normal samples in lung adenocarcinoma[J].Molecular Oncology,2019,13(6):1356-1368.  
[25] LI Y F,CHEN Y,JIN W J,et al.Analyses of MicroRNA and mRNA expression profiles reveal the crucial interaction networks and pathways for regulation of chicken breast muscle development[J].Frontiers in Genetics,2019,10:197.
[26] 禹保军,虎红红,王卫振,等.基于转录组测序技术进行差异miRNA与mRNA的关联分析[J].基因组学与应用生物学,2020:1-12.(2020-06-18).https://kns.cnki.net/KCMS/detail/45.1369.Q.20200618.0917.002.html. YU B J,HU H H,WANG W Z,et al. Association analysis of miRNA and mRNA based on transcriptome sequencing technology[J].Genomics and Applied Biology,2020:1-12.(2020-06-18).https://kns.cnki.net/KCMS/detail/45.1369.Q.20200618.0917.002.html.(in Chinese)
[27] 张宗辉,曹海虹,许崇波,等.主要组织相容性复合体Ⅰ类分子交叉递呈研究进展[J].生命科学,2020,32(5):485-493. ZHANG Z H,CAO H H,XU C B,et al.The research progress of cross-presentation for MHC Ⅰ molecules[J].Chinese Bulletin of Life Sciences,2020,32(5):485-493.(in Chinese)
[28] 秦菱涔,杜先智.结核分枝杆菌抑制吞噬体-溶酶体形成机制研究进展[J].国际呼吸杂志,2020,40(3):211-214. QIN L C,DU X Z.Research Progress on mechanism of inhibition of phagosome-lysosome formation by Mycobacterium tuberculosis[J].International Journal of Respiration,2020,40(3):211-214.(in Chinese)
[29] WALPOLE G F W,PLUMB J D,CHUNG D,et al.Inactivation of Rho GTPases by Burkholderia cenocepacia induces a wash-mediated actin polymerization that delays phagosome maturation[J].Cell Reports,2020,31(9):107721.
[30] AGRAWAL A,KHAN M J,GRAUGNARD D E,et al.Prepartal energy intake alters blood polymorphonuclear leukocyte transcriptome during the peripartal period in holstein cows[J].Bioinformatics and Biology Insights,2017,11:1177932217704667.
文章导航

/