动物营养学报 >

2021 , Vol. 33 >Issue 2: 1111 - 1119

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.02.051

分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

壳寡糖对奶牛外周血单个核细胞抗氧化功能的促进作用

  • 郑亚光 ,
  • 齐敬宇 ,
  • 张博綦 ,
  • 赵艳丽 ,
  • 郭晓宇 ,
  • 史彬林 ,
  • 闫素梅
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  • 内蒙古农业大学动物科学学院, 呼和浩特 010018
郑亚光(1990-),男,内蒙古包头人,博士研究生,从事反刍动物营养研究。E-mail:zhengyaguangg@163.com

收稿日期: 2020-07-15

  网络出版日期: 2021-02-04

基金资助

国家自然科学基金项目（31672463）

收起

Promoting Effect of Chitooligosaccharides on Antioxidant Function of Peripheral Blood Mononuclear Cells of Dairy Cows

  • ZHENG Yaguang ,
  • QI Jingyu ,
  • ZHANG Boqi ,
  • ZHAO Yanli ,
  • GUO Xiaoyu ,
  • SHI Binlin ,
  • YAN Sumei
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  • College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2020-07-15

  Online published: 2021-02-04

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摘要

本试验以奶牛外周血单个核细胞（PBMC）为模型，旨在研究不同剂量壳寡糖（COS）对细胞活力、抗氧化功能和炎症因子含量的影响。采用单因素随机试验设计，将体外分离得到的PBMC随机分为5组，每组6个重复，对照组（CON组）细胞培养液中不添加COS；COS40、COS80、COS160和COS320组在细胞培养液中分别添加40、80、160和320 μg/mL的COS，在培养箱中培养48 h。结果表明：体外添加COS对PBMC抗氧化功能的促进效果呈剂量效应，可增强抗氧化酶如硫氧还蛋白还原酶（TrxR）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）的活性，其中以160 μg/mL的COS具有较好的效果。COS可抑制炎症因子白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）的产生，降低诱导型一氧化氮合酶（iNOS）的活性与一氧化氮（NO）的含量，并呈现剂量依赖性，以160 μg/mL COS具有较强的抑制作用，而320 μg/mL COS的抑制作用减弱。体外添加COS抑制了核因子-κB（NF-κB）p50和NF-κB p65的基因表达，并且呈现剂量依赖性。综上可知，COS可通过抑制NF-κB信号通路活性降低iNOS与炎症因子的基因表达以及NO的生成，进而提高PBMC的抗氧化功能。

关键词： 壳寡糖; 外周血单个核细胞; 抗氧化功能; 一氧化氮; 核因子-κB

本文引用格式

郑亚光 , 齐敬宇 , 张博綦 , 赵艳丽 , 郭晓宇 , 史彬林 , 闫素梅 . 壳寡糖对奶牛外周血单个核细胞抗氧化功能的促进作用[J]. 动物营养学报, 2021 , 33(2) : 1111 -1119 . DOI: 10.3969/j.issn.1006-267x.2021.02.051

Abstract

In this experiment, dairy cows' peripheral blood mononuclear cells (PBMC) were used as a model to study the effects of different doses of chitooligosaccharides (COS) on cell viability, antioxidant function and inflammatory factor contents. The test adopted a single-factor randomized test design, and the isolated PBMC in vitro were randomly divided into 5 groups with 6 replicates per group, one was the control group (CON group), and COS was not added to the cell culture medium; the remaining 4 groups were COS40, COS80, COS160 and COS320 groups, in which, the 40, 80, 160 and 320 μg/mL COS were added to the cell culture medium, respectively. The PBMC were incubated for 48 h in incubator. The results showed that the in vitro addition of COS had a dose-responsive effect on the antioxidant function of PBMC, which enhanced the activities of antioxidant enzymes thioredoxin reductase (TrxR), catalase (CAT) and superoxide dismutase (SOD). Among them, COS with 160 μg/mL had a better effect. COS inhibited the production of inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), and reduced the inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) content in a dose-dependent model. It had a good reduction effect in the COS dose of 160 μg/mL, and the inhibitory effect of 320 μg/mL COS was weakened. The in vitro addition of COS inhibited the gene expression of nuclear factor-κB (NF-κB) p50 and NF-κB p65, and in a dose-dependent model. It is suggested that COS may reduce the gene expression of iNOS and inflammatory factors and the production of NO by inhibiting the activity of NF-κB signaling pathway, and then promotes the antioxidant function of PBMC.

Key words： chitooligosaccharides; PBMC; antioxidant function; nitric oxide; nuclear factor-κB

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