实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

2种氧弹热量计测定样品总能的差异及其对能量消化率的影响

  • 杜中原 ,
  • 苏艳芳 ,
  • 陈凯旋 ,
  • 张虎 ,
  • 高庆涛 ,
  • 赵峰
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  • 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193
杜中原(1993-),男,河南永城人,硕士研究生,从事饲料养分生物学效价评定的研究。E-mail:1826080182@qq.com

收稿日期: 2020-06-28

  网络出版日期: 2021-02-04

基金资助

中国农业科学院科技创新工程(ASTIP-IAS07);温氏食品集团股份有限公司-中国农业科学院北京畜牧兽医研究所合作项目(2018-YF-01)

Difference in Two Types of Oxygen Bomb Calorimeter to Determine Gross Energy and Its Effects on Energy Digestibility of Samples

  • DU Zhongyuan ,
  • SU Yanfang ,
  • CHEN Kaixuan ,
  • ZHANG Hu ,
  • GAO Qingtao ,
  • ZHAO Feng
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  • State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2020-06-28

  Online published: 2021-02-04

Supported by

 

摘要

本试验旨在研究2种氧弹热量计测定样品总能(GE)的差异及其对能量消化率的影响。采用两样本比较试验设计,考察2种氧弹热量计测定4个饲粮、3个饲料原料及4个粪样的GE与其变异系数的差异,以及测定饲粮、饲料原料和仿生消化未消化残渣GE后计算的酶水解物能值(EHGE)和能量消化率的差异。在GE日内与日间变异的测定中,在每天09:00—21:00,每3 h测定1次样品的GE值,共测定4次,累计测定4 d。在仿生消化法饲粮能量消化率的测定中,每个样品的仿生消化设10个重复,未消化残渣绝干后随机分成2组。2种氧弹热量计测定饲粮或饲料原料GE,每个样品重复测定2次,对每个未消化残渣重复样品的GE测定1次。结果表明:1)2种氧弹热量计测定饲粮、饲料原料的GE存在显著差异(P<0.01),而粪样的GE差异不显著(P>0.05)。2)与氧弹热量计型号1相比,氧弹热量计型号2测定粪样GE的日内、日间及总变异系数相对较小。3)2种氧弹热量计测定样品及残渣的GE,由此计算的饲粮3、棉籽粕的EHGE在2种氧弹热量计之间有显著差异(P<0.05),饲粮3及3个饲料原料的能量消化率在2种氧弹热量计之间有显著差异(P<0.05)。由此可见,2种型号氧弹热量计在测定饲粮及饲料原料的GE上存在差异,在测定粪便GE的重复性上差异较大。2种氧弹热量计间测定样品GE的差异引起了部分样品EHGE及能量消化率的差异,但这一差异程度远低于GE差异的程度。

本文引用格式

杜中原 , 苏艳芳 , 陈凯旋 , 张虎 , 高庆涛 , 赵峰 . 2种氧弹热量计测定样品总能的差异及其对能量消化率的影响[J]. 动物营养学报, 2021 , 33(2) : 1128 -1136 . DOI: 10.3969/j.issn.1006-267x.2021.02.053

Abstract

The objective of this experiment was to investigate the difference in the two types of oxygen bomb calorimeter to determine gross energy (GE) and its effects on energy digestibility of samples. A two-sample comparison experimental design was adopted to study the difference in GE and its coefficient of variation for four diets, three feed ingredients and four feces determined with two types of oxygen bomb calorimeters, as well as the difference in the enzymatic hydrolysate gross energy (EHGE) and energy digestibility calculated from the GE of diets, feed ingredients and corresponding residues after simulated digestion. In the determination of intra-day and inter-day variation, the GE of the samples were measured four times daily with once every 3 h from 09:00 to 21:00 and continued to measure for 4 d. In the determination of diet energy digestibility in the simulated digestion, each sample contained 10 replicates. The undigested residues were dried and randomly divided into 2 groups. The two types of oxygen bomb calorimeter were used to measure the GE of each diets or feed ingredients in duplicate, and the GE of each undigested residues only once. The results showed as follows: 1) there were significant differences between the GE measured by the two types of oxygen bomb calorimeter for each of diets and feed ingredients (P<0.05), while no difference was observed between the GE of feces. 2) The smaller intra-day, inter-day and the total coefficients of variation of GE for feces were observed in the oxygen bomb calorimeter type 2 compared with oxygen bomb calorimeter type 1. 3) There were significant differences in the EHGE in each of diet 3 and cottonseed meal and energy digestibilities in each of diet 3 and three feed ingredients calculated from the GE of samples and undigested residues measured by two types of oxygen bomb calorimeter (P<0.05). In conclusion, two types of oxygen bomb calorimeter present a difference in GE measurement for diets and feed ingredients, and present a great difference in the repeatability of fecal GE. Differences in GE measured by two types of oxygen bomb calorimeter lead to differences in EHGE and energy digestibility in some samples, but the extent of this difference is much less than the difference in GE.

参考文献

[1] ISO.ISO 9831-1998 Animal feeding stuffs,animal products,and faeces or urine-determination of gross calorific value bomb calorimeter method[S].Switzerland:ISO,1998.
[2] 蔡阿敏,李鹏涛,范逸婷,等.粉碎粒度对氧弹式量热法测定全混合日粮总能的影响[J].动物营养学报,2019,31(9):4194-4199. CAI A M,LI P T,FAN Y T,et al.Effects of grinding particle size on gross energy of total mixed ration measured by oxygen bomb calorimetry[J].Chinese Journal of Animal Nutrition,2019,31(9):4194-4199.(in Chinese)
[3] ROJAS O J,STEIN H H.Effects of reducing the particle size of corn grain on the concentration of digestible and metabolizable energy and on the digestibility of energy and nutrients in corn grain fed to growing pigs[J].Livestock Science,2015,181:187-193.
[4] 赖建辉,田超.饲料总能和粪能的测定技术[J].饲料工业,1994,15(8):32-34. LAI J H,TIAN C.Technique for determining gross energy of feed and feces[J].Feed Industry,1994,15(8):32-34.(in Chinese)
[5] SHEN J F,ZHU S G,LIU X Z,et al.Measurement of heating value of rice husk by using oxygen bomb calorimeter with benzoic acid as combustion adjuvant[J].Energy Procedia,2012,17:208-213.
[6] SIBBALD I R,MORSE P M.Variation among gross energy values measured by two modes of adiabatic oxygen bomb calorimetry[J].Poultry Science,1982,61(5):994-997.  
[7] GAO Q T,ZHAO F,DANG F K,et al.Effect of corn particle size on the particle size of intestinal digesta or feces and nutrient digestibility of corn-soybean meal diets for growing pigs[J].Animals,2020,10(5):876.
[8] 党方坤.生长猪肠内消化酶的纯化与肠液体外模拟的研究[D].硕士学位论文.北京.中国农业科学院,2018. DANG F K.Study on purification of digestive enzymes in intestinal tract and in vitro simulation of intestinal fluid for growing pigs[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2018.(in Chinese)
[9] 赵峰,张宏福,张子仪.单胃动物仿生消化系统操作手册[M].2版.北京:中国农业科学院,2011. ZHAO F,ZHANG H F,ZHANG Z Y.Manual of simulated digestion system for monogastric animal[M].2nd ed.Beijing:Chinese Academy of Agricultural Sciences,2011.(in Chinese)
[10] 蒋红卫,夏结来.基于样本变异系数的组间与组内变异统计量[C]//中国卫生统计学术交流大会论文集.武汉:[s.n.],2006:1-7. JIANG H W,XIA J L.Statistics for inter and intra group variation based on coefficient of variation in samples[C]//Proceedings of China health statistics conference.Wuhan:[s.n.],2006:1-7. (in Chinese)
[11] 李仲玉,徐良梅,王洪亮.PARR 6300氧弹量热仪故障排除及使用中应注意的问题[J].饲料博览,2009(9):28-30. LI Z Y,XU L M,WANG H L.Troubleshooting and attention in the use for PARR 6300 oxygen bomb calorimeter.[J].Feed Review,2009(9):28-30.(in Chinese)
[12] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007. ZHANG L Y.Feed analysis and quality test technology[M].3 ed.Beijing:China Agricultural University Press,2007.(in Chinese)
[13] AZAM F,QAISRANI S N,KHALIQUE A,et al.Exploring nutritive profile,metabolizable energy,protein,and digestible amino acids contents of indigenous protein sources of different locations for male broilers[J].Poultry Science,2019,98(10):4664-4672.  
[14] ALI ABBASI M,GHAZANFARI S,SHARIFI S D,et al.Influence of dietary plant fats and antioxidant supplementations on performance,apparent metabolizable energy and protein digestibility,lipid oxidation and fatty acid composition of meat in broiler chicken[J].Veterinary Medicine and Science,2020,6(1):54-68.  
[15] XIE F,LI Y K,ZHAO J B,et al.Comparative digestibility of energy and nutrients in four fibrous ingredients fed to barrows at three different initial body weights[J].Canadian Journal of Animal Science,2018,99(2):315-325.
[16] JAWORSKI N W,STEIN H H.Disappearance of nutrients and energy in the stomach and small intestine,cecum,and colon of pigs fed corn-soybean meal diets containing distillers dried grains with solubles,wheat middlings,or soybean hulls[J].Journal of Animal Science,2017,95(2):727-739.  
[17] MA D L,MA X K,LIU L,et al.Chemical composition,energy,and amino acid digestibility in 7 cottonseed co-products fed to growing pigs[J].Journal of Animal Science,2018,96(4):1338-1349.  
[18] QUEMENEUR K,MONTAGNE L,LEGALL M,et al.Relation between feeding behaviour and energy metabolism in pigs fed diets enriched in dietary fibre and wheat aleurone[J].Animals,2020,14(3):508-519.
[19] TANG X P,XIANG R,CHEN S J,et al.Effects of fermented cottonseed meal and enzymatic hydrolyzed cottonseed meal on amino acid digestibility and metabolic energy in white leghorn rooster[J].Pakistan Journal of Zoology,2018,50(3):957-962.
[20] 马艳,谭卫红,沈娟章,等.新型氧弹量热仪测定谷物的热值[J].生命科学仪器,2013,11(3):42-44. MA Y,TAN W H,SHEN J Z,et al.Determination of the heat values of several cereals with bobm-calorimeter[J].Life Science Instruments,2013,11(3):42-44. (in Chinese)
[21] 娄瑞颍,刘国华,张玉萍,等.玉米理化品质及其鸡代谢能的变异研究[J].饲料工业,2011,32(16):34-38. LOU R Y,LIU G H,ZHANG Y P,et al.Physical and chemical quality of corn and its variation research of metabolizable energy in broilers[J].Feed Industry,2011,32(16):34-38.(in Chinese)
[22] 李全丰.中国玉米猪有效营养成分预测方程的构建[D].博士学位论文.北京:中国农业大学,2014. LI Q F.Establish prediction modeling on available nutrients with Chinese corn for pigs[D].Ph.D.Thesis.Beijing:China Agricultural University.2014.(in Chinese)
[23] D'ALFONSO T H,MANBECK H B,ROUSH W B.Partitioning of variance in true metabolizable energy determinations:an example using wheat data[J].Animal Feed Science and Technology,1999,80(1):29-41.  
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