猪营养与饲料 SWINE NUTRITION AND FEED

母猪妊娠饲粮中添加甲基供体对新生仔猪肝脏糖脂代谢和线粒体功能的影响

  • 简丽 ,
  • 邹田德 ,
  • 李建军 ,
  • 谌俊 ,
  • 贺琴 ,
  • 何嘉 ,
  • 夏莹莹 ,
  • 谢飞 ,
  • 王自蕊 ,
  • 游金明
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  • 1. 江西农业大学动物科学技术学院, 江西省动物营养重点实验室, 江西省营养饲料开发工程研究中心, 江西省优质安全畜禽生产产教融合重点创新中心, 南昌 330045;
    2. 江西省赣州市畜牧研究所, 赣州 341000
简丽(1996-),女,安徽滁州人,硕士研究生,从事猪营养与饲料科学研究。E-mail:1041005739@qq.com

收稿日期: 2021-01-31

  网络出版日期: 2021-09-18

基金资助

国家重点研发计划项目(2018YFD0500401);江西省生猪产业技术体系(JXARS-03-营养与饲料岗)

Effects of Methyl-Donor Supplementation in Diets for Pregnant Sows on Hepatic Glycolipid Metabolism and Mitochondrial Function of Newborn Piglets

  • JIAN Li ,
  • ZOU Tiande ,
  • LI Jianjun ,
  • CHEN Jun ,
  • HE Qin ,
  • HE Jia ,
  • XIA Yingying ,
  • XIE Fei ,
  • WANG Zirui ,
  • YOU Jinming
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  • 1. Jiangxi Province Key Laboratory of Animal Nutrition, Jiangxi Province Nutrition & Feed Development Engineering Research Center, Jiangxi Province Key Innovation Center of Integration in Production and Education for High-Quality and Safe Livestock and Poultry, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China;
    2. Ganzhou Institute of Animal Husbandry, Ganzhou 341000, China

Received date: 2021-01-31

  Online published: 2021-09-18

Supported by

 

摘要

本试验旨在研究母猪妊娠饲粮中添加甲基供体对新生仔猪肝脏糖脂代谢和线粒体功能的影响。将平均体重为(102.8±6.3)kg的"杜洛克×二花脸"初胎母猪在人工授精后分为2组,分别饲喂基础饲粮(对照组,n=21)和添加甲基供体(4 700 mg/kg蛋氨酸、16.3 mg/kg叶酸、2 230 mg/kg胆碱、0.15 mg/kg维生素B12和1 180 mg/kg维生素B6)的基础饲粮(甲基供体组,n=22)。饲养试验从母猪配种开始至母猪分娩结束。母猪分娩结束后,称取新生仔猪重,然后每组选取8窝,每窝选取1头仔猪(每组共选取8头仔猪)进行屠宰采样,测定新生仔猪内脏器官重量,并采集肝脏,用于测定肝脏糖脂代谢和线粒体功能指标。结果表明:1)相比对照组,母猪妊娠饲粮中添加甲基供体对新生仔猪个体重和肝脏重量无显著影响(P>0.05),但显著提高了新生仔猪脾脏重量(P<0.05)。2)相比对照组,母猪妊娠饲粮中添加甲基供体显著提高了新生仔猪肝脏中糖原(Gly)含量、磷酸烯醇式丙酮酸羧激酶(PEPCK)与葡萄糖-6-磷酸酶(G6PC)活性(P<0.05),并显著降低了新生仔猪肝脏中甘油三酯(TG)和总胆固醇(TC)含量(P<0.05)。3)相比对照组,母猪妊娠饲粮中添加甲基供体显著上调了新生仔猪肝脏糖异生相关酶如PEPCK1和G6PC的mRNA和蛋白相对表达量(P<0.05),显著下调了新生仔猪肝脏固醇调节元件结合蛋白-1C(SREBP-1C)和硬脂酰辅酶A去饱和酶(SCD)等脂质合成相关基因的mRNA相对表达量(P<0.05),同时还显著上调了新生仔猪肝脏过氧化物酶体增殖物激活受体α(PPARα)和脂蛋白脂酶(LPL)等肝脏脂质分解相关基因的mRNA相对表达量(P<0.05)。4)相比对照组,母猪妊娠饲粮中添加甲基供体显著提高了新生仔猪肝脏三磷酸腺苷(ATP)、氧化型辅酶Ⅰ(NAD+)、线粒体DNA(mtDNA)含量以及柠檬酸合酶(CS)和琥珀酸脱氢酶(SDH)活性(P<0.05)。5)相比对照组,母猪妊娠饲粮中添加甲基供体显著提高了新生仔猪肝脏过氧化物酶体增殖物激活受体γ辅激活因子-1α(PGC-1α)、细胞核呼吸因子-1(NRF-1)的mRNA相对表达量(P<0.05)。综上所述,母猪妊娠饲粮中添加甲基供体可抑制新生仔猪肝脏脂质沉积,促进新生仔猪糖异生,改善新生仔猪肝脏糖脂代谢和线粒体功能。

本文引用格式

简丽 , 邹田德 , 李建军 , 谌俊 , 贺琴 , 何嘉 , 夏莹莹 , 谢飞 , 王自蕊 , 游金明 . 母猪妊娠饲粮中添加甲基供体对新生仔猪肝脏糖脂代谢和线粒体功能的影响[J]. 动物营养学报, 2021 , 33(9) : 4920 -4930 . DOI: 10.3969/j.issn.1006-267x.2021.09.013

Abstract

This study aimed to investigate the effects of methyl-donor supplementation in diets for pregnant sows on hepatic glycolipid metabolism and mitochondrial function of newborn piglets. "Duroc×Erhuanlian" crossbred gilts with the body weight of (102.8±6.3) kg were randomly allotted two groups after artificial insemination (21 and 22 gilts for control group and methyl-donor group, respectively). Gilts in the methyl-donor group and control group were fed a basal diet supplemented with or without methyl-donor, and the methyl-donor included 4 700 mg/kg methionine, 16.3 mg/kg folate, 2 230 mg/kg choline, 0.15 mg/kg vitamin B12 and 1 180 mg/kg vitamin B6. The feeding trail were conducted until farrowing. After farrowing, the newborn piglets were weighted, and 8 litters were selected and 1 neonate per litter were slaughtered for liver samples collection. The visceral organs of newborns were separated and weighed, and liver samples were collected for the analysis of glycolipid metabolism and mitochondrial function indices. The results show as follows:1) compared with the control group, methyl-donor supplementation in the pregnant sows' diet did not significantly affect the individual weight and liver weight of newborn piglets (P>0.05), but significantly increased the spleen weight of newborn piglets (P<0.05). 2) Compared with the control group, methyl-donor supplementation in the pregnant sows' diet significantly increased the hepatic glycogen content, phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6PC) activities of newborn piglets (P<0.05), and significantly decreased the hepatic triglyceride and total cholesterol contents of newborn piglets (P<0.05). 3) Compared with the control group, methyl-donor supplementation in the pregnant sows' diet significantly down-regulated the mRNA and protein relative expression levels of hepatic lipid-synthesis-related genes such as sterol regulatory element-binding protein-1C (SREBP-1C) and stearoyl coenzyme A desaturase (SCD) (P<0.05), and significantly up-regulated hepatic gluconeogenesis-related enzymes such as PEPCK1 and G6PC at both mRNA and protein relative expression levels, as well as the mRNA relative expression levels of hepatic lipidolysis-related genes such as peroxisome proliferator-activated receptor-α (PPARα) and lipoprotein lipase (LPL) of newborn piglets (P<0.05). 4) Compared with the control group, methyl-donor supplementation in the pregnant sows' diet significantly increased the contents of adenosine triphosphate (ATP), NAD+ and mitochondrial DNA (mtDNA), and the activities of citrate synthase (CS) and succinate dehydrogenase (SDH) in the liver of newborn piglets (P<0.05). 5) Compared with the control group, methyl-donor supplementation in the pregnant sows' diet significantly up-regulated the mRNA relative expression levels of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) and nuclear respiratory factor-1 (NRF-1) in liver of newborn piglets (P<0.05). Overall, methyl-donor supplementation in the pregnant sows' diet can inhibit the hepatic lipid synthesis, promote the gluconeogenesis, and thus improve the hepatic glycolipid metabolism and mitochondrial function of newborn piglets.

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