猪营养与饲料 SWINE NUTRITION AND FEED

妊娠-哺乳期饲粮添加甜菜碱盐酸盐对母猪粪便菌群组成及代谢产物含量的影响

  • 马翠 ,
  • 高乾坤 ,
  • 祝倩 ,
  • 宋明彤 ,
  • 耿梅梅 ,
  • 韩强 ,
  • 孔祥峰
展开
  • 1. 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 动物营养生理与代谢过程湖南省重点实验室, 长沙 410125;
    2. 中国科学院大学, 北京 100049;
    3. 山东祥维斯生物科技股份有限公司, 潍坊 261000
马翠(1995-),女,河南商丘人,硕士研究生,从事单胃动物营养研究。E-mail:macui016@163.com

收稿日期: 2021-02-24

  网络出版日期: 2021-09-18

基金资助

国家重点研发计划课题(2018YFD0500404);中科院王宽诚率先人才计划"产研人才扶持项目";山东祥维斯生物科技股份有限公司横向课题(20190101-ISA-XWS)

Effects of Dietary Betaine Hydrochloride Supplementation on Fecal Microbiota Composition and Metabolites Contents of Sows During Pregnancy and Lactation

  • MA Cui ,
  • GAO Qiankun ,
  • ZHU Qian ,
  • SONG Mingtong ,
  • GENG Meimei ,
  • HAN Qiang ,
  • KONG Xiangfeng
Expand
  • 1. Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. Sunwin Biotech Shandong Co., Ltd., Weifang 261000, China

Received date: 2021-02-24

  Online published: 2021-09-18

Supported by

 

摘要

本试验旨在研究妊娠-哺乳期饲粮添加甜菜碱盐酸盐对母猪粪便菌群组成及代谢产物含量的影响。选用3~7胎次妊娠巴马香猪40头,随机分为2组,每组20头,单栏饲养。对照组饲喂基础饲粮,试验组在基础饲粮中添加3.5 kg/t的甜菜碱盐酸盐。试验期为母猪配种后第3天至分娩后第21天。分别在妊娠第105天和哺乳第21天,取母猪新鲜粪便样品用于微生物16S rDNA测序以及短链脂肪酸、生物胺、吲哚和粪臭素含量测定。结果表明:与对照组相比,饲粮添加甜菜碱盐酸盐显著增加了妊娠第105天母猪粪便迷踪菌门(Elusimicrobia)和粪芽孢菌属(Coprobacillus)相对丰度以及尸胺含量(P<0.05),哺乳第21天母猪粪便微生物Chao1和ACE指数和猪粪厌氧原体属(Anaeroplasma)相对丰度以及腐胺、亚精胺含量(P<0.05)。皮尔曼相关性分析发现,粪便厌氧原体属相对丰度与亚精胺含量呈正相关(P<0.05),与异戊酸和异丁酸含量呈负相关(P<0.05)。KEGG通路分析发现,与对照组相比,饲粮添加甜菜碱盐酸盐上调了妊娠第105天核苷酸代谢、聚糖生物合成与代谢、酶家族通路,上调了哺乳第21天异生素生物降解与代谢、萜类与多酮类代谢、脂代谢和其他氨基酸代谢通路。由此可见,饲粮添加甜菜碱盐酸盐可改变妊娠-哺乳母猪肠道菌群组成及代谢产物含量,进而改善机体代谢。

本文引用格式

马翠 , 高乾坤 , 祝倩 , 宋明彤 , 耿梅梅 , 韩强 , 孔祥峰 . 妊娠-哺乳期饲粮添加甜菜碱盐酸盐对母猪粪便菌群组成及代谢产物含量的影响[J]. 动物营养学报, 2021 , 33(9) : 4931 -4942 . DOI: 10.3969/j.issn.1006-267x.2021.09.014

Abstract

This experiment was conducted to study the effects of dietary betaine hydrochloride supplementation on fecal microbiota composition and metabolites contents of sows during pregnancy and lactation. Forty pregnant Bama mini-pigs with three to seven parities were used and randomly allocated to two groups with 20 pigs per group, and fed individually. Sows in the control group were fed a basal diet and others in the experiment group were fed the basal diet supplemented with 3.5 kg/t betaine hydrochloride. The trial period was from day 3 after mating to postpartum day 21. At day 105 of pregnancy and day 21 of lactation, the fresh fecal samples of sows were collected for microbial 16S rDNA sequencing, as well as the determination of short-chain fatty acids, bioamines, indole and skatole contents. The results showed that compared with the control group, dietary betaine hydrochloride supplementation significantly increased the fecal Elusimicrobia and Coprobacillus relative abundances and cadaverine content at day 105 of pregnancy (P<0.05), and significantly increased the fecal microbiota Chao1, ACE indexes, fecal Anaeroplasma relative abundance and putrescine, spermidine contents at day 21 of lactation (P<0.05). The Pearson correlation analysis found that the fecal Anaeroplasma relative abundance was positively correlated with spermidine content (P<0.05), and was negatively correlated with isobutyrate and isovalerate contents (P<0.05). The KEGG pathway analysis showed that compared with the control group, dietary betaine hydrochloride supplementation up-regulated the nucleotide metabolism, glycan biosynthesis and metabolism and enzyme families pathways at day 105 of pregnancy, as well as xenobiotics biodegradation and metabolism, metabolism of terpenoids and polyketides, metabolism of other amino acids and lipid metabolism pathways at day 21 of lactation. In conclusion, dietary betaine hydrochloride supplementation can alter fecal microbiota composition and metabolites contents of sows during pregnancy and lactation, and then improve the host's metabolism.

参考文献

[1] CRAIG S A S.Betaine in human nutrition[J].The American Journal of Clinical Nutrition,2004,80(3):539-549.  
[2] RATRIYANTO A,MOSENTHIN R,BAUER E,et al.Metabolic,osmoregulatory and nutritional functions of betaine in monogastric animals[J].Asian-Australasian Journal of Animal Science,2009,22(10):1461-1476.  
[3] WANG H C,LI S S,FANG S L,et al.Betaine improves intestinal functions by enhancing digestive enzymes,ameliorating intestinal morphology,and enriching intestinal microbiota in high-salt stressed rats[J].Nutrients,2018,10(7):907.
[4] DICKINSON H,ELLERY S,IRELAND Z,et al.Creatine supplementation during pregnancy:summary of experimental studies suggesting a treatment to improve fetal and neonatal morbidity and reduce mortality in high-risk human pregnancy[J].BMC Pregnancy and Childbirth,2014,14:150.
[5] CORBETT H E,DUBÉ C D,SLOW S,et al.Uptake of betaine into mouse cumulus-oocyte complexes via the SLC7A6 isoform of y+L transporter[J].Biology of Reproduction,2014,90(4):81.
[6] GRESSE R,CHAUCHEYRAS-DURAND F,FLEURY M A,et al.Gut microbiota dysbiosis in postweaning piglets:understanding the keys to health[J].Trends in Microbiology,2017,25(10):851-873.  
[7] ZIAR H,GÉRARD P,RIAZI A.Effect of prebiotic carbohydrates on growth,bile survival and cholesterol uptake abilities of dairy-related bacteria[J].Journal of the Science of Food and Agriculture,2014,94(6):1184-1190.  
[8] DEBELIUS J,SONG S J,VAZQUEZ-BAEZA Y,et al.Tiny microbes,enormous impacts:what matters in gut microbiome studies?[J].Genome Biology,2016,17(1):217.
[9] MA C,ZHANG W H,GAO Q K,et al.Dietary synbiotic alters plasma biochemical parameters and fecal microbiota and metabolites in sows[J].Journal of Functional Foods,2020,75:104221.
[10] 高乾坤,马翠,孔祥峰,等.饲粮添加甜菜碱对巴马香猪繁殖性能、初乳成分及血浆代谢物和繁殖激素含量的影响[J].动物营养学报,2020,32(2):646-653. GAO Q K,MA C,KONG X F,et al.Effects of dietary betaine supplementation on reproductive performance,colostrum composition and plasma metabolite and reproductive hormone contents of Bama mini-pigs[J].Chinese Journal of Animal Nutrition,2020,32(2):646-653.(in Chinese)
[11] HU L,LIU Y,YAN C,et al.Postnatal nutritional restriction affects growth and immune function of piglets with intra-uterine growth restriction[J].The British Journal of Nutrition,2015,114(1):53-62.  
[12] DESANTIS T Z,HUGENHOLTZ P,LARSEN N,et al.Greengenes,a chimera-checked 16S rRNA gene database and workbench compatible with ARB[J].Applied and Environmental Microbiology,2006,72(7):5069-5072.  
[13] LI H,QU J P,LI T T,et al.Diet simplification selects for high gut microbial diversity and strong fermenting ability in high-altitude pikas[J].Applied Microbiology and Biotechnology,2018,102(15):6739-6751.  
[14] CHEN Q,WANG Y,JIAO F Z,et al.Betaine inhibits Toll-like receptor 4 responses and restores intestinal microbiota in acute liver failure mice[J].Scientific Reports,2020,10(1):21850.
[15] ZHENG H,DIETRICH C,RADEK R,et al.Endomicrobium proavitum,the first isolate of Endomicrobia class. nov. (phylum Elusimicrobia)-an ultramicrobacterium with an unusual cell cycle that fixes nitrogen with a group Ⅳ nitrogenase[J].Environmental Microbiology,2016,18(1):191-204.  
[16] WANG K,NAN X M,TONG J J,et al.Steam explosion pretreatment changes ruminal fermentation in vitro of corn stover by shifting archaeal and bacterial community structure[J].Frontiers in Microbiology,2020,11:2027.
[17] BRAHE L K,LE CHATELIER E,PRIFTI E,et al.Specific gut microbiota features and metabolic markers in postmenopausal women with obesity[J].Nutrition & Diabetes,2015,5(6):e159.
[18] BELLER A,KRUGLOV A,DUREK P,et al.Specific microbiota enhances intestinal IgA levels by inducing TGF-β in T follicular helper cells of Peyer's patches in mice[J].European Journal of Immunology,2020,50(6):783-794.  
[19] GUTZEIT C,MAGRI G,CERUTTI A.Intestinal IgA production and its role in host-microbe interaction[J].Immunological Reviews,2014,260(1):76-85.  
[20] BOETS E,GOMAND S V,DEROOVER L,et al.Systemic availability and metabolism of colonic-derived short-chain fatty acids in healthy subjects:a stable isotope study[J].The Journal of Physiology,2017,595(2):541-555.  
[21] YAN H,AJUWON K M.Butyrate modifies intestinal barrier function in IPEC-J2 cells through a selective upregulation of tight junction proteins and activation of the Akt signaling pathway[J].PLoS One,2017,12(6):e0179586.
[22] YOSHIKAWA S,ARAOKA R,KAJIHARA Y,et al.Valerate production by Megasphaera elsdenii isolated from pig feces[J].Journal of Bioscience and Bioengineering,2018,125(5):519-524.  
[23] FUSI E,BALDI A,CHELI F,et al.Effects of putrescine,cadaverine,spermine,spermidine and β-phenylethylamine on cultured bovine mammary epithelial cells[J].Italian Journal of Animal Science,2008,7(2):131-140.  
[24] 胡彩虹,俞颂东,许梓荣.猪粪便细菌群作用下3-甲基吲哚(粪臭素)和吲哚形成的研究[J].中国畜牧杂志,2002,38(5):10-11. HU C H,YU S D,XU Z R.Effect of mixed population of pig fecal bacteria on 3-methylindole (skatole) and indole production[J].Chinese Journal of Animal Science,2002,38(5):10-11.(in Chinese)
[25] LIU H Y,HOU R,YANG G Q,et al.In vitro effects of inulin and soya bean oligosaccharide on skatole production and the intestinal microbiota in broilers[J].Journal of Animal Physiology and Animal Nutrition,2018,102(3):706-716.  
[26] YADAV P N,LIU Z,RAFI M M.A diarylheptanoid from lesser galangal (Alpinia officinarum) inhibits proinflammatory mediators via inhibition of mitogen-activated protein kinase,p44/42,and transcription factor nuclear factor-kappa B[J].The Journal of Pharmacology and Experimental Therapeutics,2003,305(3):925-931.  
[27] MA C,GAO Q K,ZHANG W H,et al.Alterations in the blood parameters and fecal microbiota and metabolites during pregnant and lactating stages in Bama mini pigs as a model[J].Mediators of Inflammation,2020:8829072.
[28] WALLACE R J.Ruminal microbial metabolism of peptides and amino acids[J].The Journal of Nutrition,1996,126(4 Suppl):1326S-1334S.
文章导航

/