反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

不同浓度纤维素酶菌剂处理对小麦、青稞和油菜黄贮体外发酵特性的影响

  • 李斌 ,
  • 旦增曲珍 ,
  • 白玛央金 ,
  • 马金英 ,
  • 金涛
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  • 西藏自治区农牧科学院畜牧兽医研究所, 拉萨 850009
李斌(1989-),男,黑龙江哈尔滨人,助理研究员,硕士,从事奶牛育种研究。E-mail:276504821@qq.com

收稿日期: 2021-02-15

  网络出版日期: 2021-09-18

基金资助

西藏农作物秸秆综合利用技术集成与示范项目(XZ201901NB07)

Effects of Different Concentrations of Cellulase Agent Treatment on Fermentation Characteristics of Wheat, Highland Barley and Rape Yellow Storages

  • LI Bin ,
  • Danzengquzhen ,
  • Baimayangjin ,
  • MA Jinying ,
  • JIN Tao
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  • Academy of Agricultural and Animal Sciences in Tibet Autonomous, Lhasa 850009, China

Received date: 2021-02-15

  Online published: 2021-09-18

Supported by

 

摘要

本试验旨在研究不同浓度纤维素酶菌剂处理对小麦、青稞和油菜黄贮体外发酵特性的影响。小麦、青稞和油菜黄贮各分为3个组,分为对照组、添加1×1010 CFU/kg纤维素分解菌组(Ⅰ组)和添加2×1010 CFU/kg纤维素分解菌组(Ⅱ组),共9个组。结果表明:1)Ⅰ组的小麦和青稞黄贮的感官评分显著高于对照组(P<0.05)。Ⅰ和Ⅱ组的小麦和青稞黄贮的乳酸、乙酸和丙酸含量显著高于对照组(P<0.05),中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)含量显著低于对照组(P<0.05)。Ⅱ组的小麦、青稞和油菜黄贮的粗蛋白质含量显著高于对照组(P<0.05)。2)Ⅰ和Ⅱ组的小麦和青稞黄贮的干物质降解率(DMD)显著高于对照组(P<0.05)。Ⅰ和Ⅱ组的小麦黄贮的中性洗涤纤维降解率(NDFD)显著高于对照组(P<0.05),Ⅰ组的青稞黄贮的NDFD显著高于对照组和Ⅱ组(P<0.05)。3)Ⅰ和Ⅱ组的小麦和青稞黄贮的总挥发性脂肪酸(TVFA)含量显著高于对照组(P<0.05)。Ⅱ组的小麦黄贮的丁酸含量显著低于对照组和Ⅰ组(P<0.05)。4)Ⅰ和Ⅱ组的小麦和青稞黄贮的48 h总产气量显著高于对照组(P<0.05)。由此可见,添加纤维素分解菌可增加小麦、青稞和油菜黄贮的DMD、NDFD和产气量,促进饲料的消化降解,促进黄贮体外发酵,且添加量以2×1010 CFU/kg为宜。

本文引用格式

李斌 , 旦增曲珍 , 白玛央金 , 马金英 , 金涛 . 不同浓度纤维素酶菌剂处理对小麦、青稞和油菜黄贮体外发酵特性的影响[J]. 动物营养学报, 2021 , 33(9) : 5016 -5024 . DOI: 10.3969/j.issn.1006-267x.2021.09.022

Abstract

This experiment was conducted to investigate the effects of different concentrations of cellulase agent treatment on fermentation characteristics of wheat, highland barley and rape yellow storages. The wheat, highland barley and rape yellow storages were divided into 3 groups, which were the control group, adding 1×1010 CFU/kg cellulolytic bacteria group (group Ⅰ) and adding 2×1010 CFU/kg cellulolytic bacteria group (group Ⅱ), there were 9 groups. The results showed as follows:1) the sensory score of wheat and highland barley yellow storages of group Ⅰ was significantly higher than that of the control group (P<0.05). The contents of lactic acid, acetic acid and propionic acid in wheat and highland barley yellow storages of groups Ⅰ and Ⅱ were significantly higher than that of the control group (P<0.05), and the contents of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were significantly lower than that of the control group (P<0.05). The content of crude protein in wheat, highland barley and rape yellow storages was significantly higher than that of the control group (P<0.05). 2) The dry matter degradation rate (DMD) of wheat and highland barley yellow storages of groups Ⅰ and Ⅱ was significantly higher than that of the control group (P<0.05). The neutral detergent fiber degradation rate (NDFD) of wheat yellow storage of groups Ⅰ and Ⅱ was significantly higher than that of the control group (P<0.05), and the NDFD of highland barley yellow storage of group Ⅰ was significantly higher than that of the control group and group Ⅱ (P<0.05). 3) The content of total volatile fatty acids (TVFA) in wheat and highland barley yellow storages of groups Ⅰ and Ⅱ was significantly higher than that of the control group (P<0.05). The content of butyric acid in wheat yellow storage of group Ⅱ was significantly lower than that of the control group and group Ⅰ (P<0.05). 4) The 48 h total gas production of wheat yellow storage of groups Ⅰ and Ⅱ was significantly higher than that of the control group (P<0.05). In conclusion, adding cellulolytic bacteria can increase the DMD, NDFD and gas production of wheat, highland barley and rape yellow storages, and promote the digestion and degradation of feed, promote the in vitro fermentation of yellow storage, and the best addition amount is 2×1010 CFU/kg.

参考文献

[1] YUAN X F,LI P P,WANG H,et al.Enhancing the anaerobic digestion of corn stalks using composite microbial pretreatment[J].Journal of Microbiology and Biotechnology,2011,21(7):746-752.  
[2] OLADOSU Y,RAFII M Y,ABDULLAH N,et al.Fermentation quality and additives:a case of rice straw silage[J].BioMed Research International,2016,2016:7985167.
[3] LARA E C,BASSO F C,DE ASSIS F B,et al.Changes in the nutritive value and aerobic stability of corn silages inoculated with Bacillus subtilis alone or combined with Lactobacillus plantarum[J].Animal Production Science,2016,56(11):1867-1874.  
[4] WANG H L,HAO W,NING T T,et al.Characterization of culturable yeast species associating with whole crop corn and total mixed ration silage[J].Asian-Australasian Journal of Animal Sciences,2018,31(2):198-207.  
[5] RANJIT N K,KUNG L,Jr.The effect of Lactobacillus buchneri,Lactobacillus plantarum,or a chemical preservative on the fermentation and aerobic stability of corn silage[J].Journal of Dairy Science,2000,83(3):526-535.  
[6] SEO J K,PARK T S,KWON I H,et al.Characterization of cellulolytic and xylanolytic enzymes of bacillus licheniformis JK7 isolated from the rumen of a native korean goat[J].Asian-Australasian Journal of Animal Sciences,2013,26(1):50-58.  
[7] WANG T Y,CHEN H L,LU M Y J,et al.Functional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses[J].Biotechnology for Biofuels,2011,4(1):24.
[8] LEE S M,GUAN L L,EUN J S,et al.The effect of anaerobic fungal inoculation on the fermentation characteristics of rice straw silages[J].Journal of Applied Microbiology,2015,118(3):565-573.  
[9] GUO G,SHEN C,LIU Q,et al.The effect of lactic acid bacteria inoculums on in vitro rumen fermentation,methane production,ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage[J].Journal of Integrative Agriculture,2020,19(3):838-847.  
[10] 张庆芳,王泽坤,姜南,等.一株瘤胃源产纤维素酶菌株的筛选、鉴定及其酶学特性研究[J].中国酿造,2019,38(4):46-50. ZHANG Q F,WANG Z K,JIANG N,et al.Screening,identification and enzymatic characteristics of a cellulose-producing strain from rumen[J].China Brewing,2019,38(4):46-50.(in Chinese)
[11] 张元庆,王栋,孟庆翔.瘤胃微生物纤维体研究进展[J].中国饲料,2005(12):2-4. ZHANG Y Q,WANG D,MENG Q X.Research progress of rumen microbial fibrous bodies[J].China Feed,2005(12):2-4.(in Chinese)
[12] MENKE K H,STEINGASS H.Estimation of the energetic feed value obtained by chemical analysis and in vitro gas production using rumen fluid[J].Animal Research and Development,1988,28:7-55.
[13] 农业部畜牧兽医司.青贮饲料质量评定标准[J].上海奶牛,1997(2):29-31. Department of Animal Husbandry and Veterinary Medicine, Ministry of Agriculture.Silage quality evaluation standard[J].Shanghai Dairy Cattle,1997(2):29-31.(in Chinese)
[14] WANG M,JANSSEN P H,SUN X Z,et al.A mathematical model to describe in vitro kinetics of H2 gas accumulation[J].Animal Feed Science and Technology,2013,184(1/2/3/4):1-16.
[15] WANG M,SUN X Z,TANG S X,et al.Deriving fractional rate of degradation of logistic-exponential (LE) model to evaluate early in vitro fermentation[J].Animal,2013,7(6):920-929.  
[16] WANG M,TANG S X,TAN Z L.Modeling in vitro gas production kinetics:derivation of logistic-exponential (LE) equations and comparison of models[J].Animal Feed Science and Technology,2011,165(3/4):137-150.
[17] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].畜牧与饲料科学,2010,31(6/7):37. FENG Z C,GAO M.Improvement of colorimetric determination of ammonia nitrogen content in rumen liquid[J].Inner Mongolia Animal Husbandry Science,2010,31(6/7):37.(in Chinese)
[18] WANG M,SUN X Z,JANSSEN P H,et al.Responses of methane production and fermentation pathways to the increased dissolved hydrogen concentration generated by eight substrates in in vitro ruminal cultures[J].Animal Feed Science and Technology,2014,194:1-11.
[19] 董晓玲,孟庆翔.丙酸和丙酸菌添加对高油玉米秸秆青贮发酵品质以及瘤胃发酵参数影响的研究[J].中国畜牧杂志,2007,43(15):35-38. DONG X L,MENG Q X.Effects of propionic acid and propionic acid bacteria on the fermentation and in vitro digestibility of high oil corn stover silage[J].Chinese Journal of Animal Science,2007,43(15):35-38.(in Chinese)
[20] 王金洛,韩正康,陈杰.山羊瘤胃微生物蛋白(MCP)与十二指肠食糜MCP浓度的比较研究[J].动物营养学报,1991,3(2):61. WANG J L,HAN Z K,CHEN J.Comparative study on the concentration of goat rumen microbial protein (MCP) and duodenal chyme MCP[J].Chinese Journal of Animal Nutrition,1991,3(2):61.(in Chinese)
[21] 孙娟娟,玉柱,薛艳林,等.不同添加剂处理对小麦秸黄贮饲料发酵品质的影响[J].中国畜牧杂志,2007,43(23):52-55. SUN J J,YU Z,XUE Y L,et al.Effects of different additive treatments on fermentation quality of wheat straw[J].Chinese Journal of Animal Science,2007,43(23):52-55.(in Chinese)
[22] 马广英.作物秸秆黄贮效果研究[D].硕士学位论文.石河子:石河子大学,2014. MA G Y.A research on the effect of ensiled crop straw[D].Master's Thesis.Shihezi:Shihezi University,2014.(in Chinese)
[23] JUÁREZ R A S,CERRILLO S M A,GUTIÉRREZ O E,et al.Assessment of the nutritional value of tropical grasses obtained from conventional analyses and in vitro gas production[J].Técnica Pecuaria en México,2009,47(1):55-67.  
[24] 杜江华,黄国欣,李彦芳,等.3种来源兼性纤维素分解菌与固氮菌混合对体外发酵的影响[J].中国畜牧杂志,2017,53(9):109-113. DU J H,HUANG G X,LI Y F,et al.Effects of three sources of facultative bacteria cellulose mixed with nitrogen-ffixing bacteria on in vitro fermentation[J].Chinese Journal of Animal Science,2017,53(9):109-113.(in Chinese)
[25] 李昊.固氮菌和纤维素分解菌的分离及混合添加对瘤胃发酵的影响[D].硕士学位论文.哈尔滨:东北农业大学,2013. LI H.The isolation of nitrogen-fixing bacteria and cellulose degrading bacteria and the effect of the two microbes on in vitro fermentation[D].Master's Thesis.Harbin:Northeast Agricultural University,2013.(in Chinese)
[26] 杜江华.瘤胃固氮菌与三种来源兼性纤维菌混合对体外发酵影响[D].硕士学位论文.哈尔滨:东北农业大学,2017. DU J H.Effects of three sources of facultative bacteria cellulose mixed with ruminal nitrogen-fixing bacteria on in vitro fermentation[D].Master's Thesis.Harbin:Northeast Agricultural University,2017.(in Chinese)
[27] ZHOU M,CHUNG Y H,BEAUCHEMIN K A,et al.Relationship between rumen methanogens and methane production in dairy cows fed diets supplemented with a feed enzyme additive[J].Journal of Applied Microbiology,2011,111(5):1148-1158.  
[28] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2004. FENG Y L.Ruminant nutrition[M].Beijing:Science Press,2004.(in Chinese)
[29] 孙满吉,杨琼,宫福臣,等.纤维素分解菌对奶牛瘤胃发酵的影响[J].中国饲料,2013(19):9-11,17. SUN M J,YANG Q,GONG F C,et al.Effects of cellulose decomposing bacteria on rumen fermentation of dairy cows[J].China Feed,2013(19):9-11,17.(in Chinese)
[30] SÁNCHEZ-SANTILLÁN P,COBOS-PERALTA M A.In vitro production of volatile fatty acids by reactivated cellulolytic bacteria and total ruminal bacteria in cellulosic substrate[J].Agrociencia,2016,50(5):565-574.
[31] 黄立敏.筛选纤维分解菌及其对体外瘤胃发酵、奶牛生产性能的影响[D].硕士学位论文.哈尔滨:东北农业大学,2014. HUANG L M.Filter cellulose-degrading bacterial the effect on rumen fermentation and production performance in dairy cows[D].Master's Thesis.Harbin:Northeast Agricultural University,2014.
[32] 乔国华,单安山.直接饲喂微生物培养物对奶牛瘤胃发酵产甲烷及生产性能的影响[J].中国畜牧兽医,2006,33(5):11-14. QIAO G H,SHAN A S.The effect of different direct-fed microbial culture on methane production in vitro and production performance in dairy cattle[J].China Animal Husbandry & Veterinary Medicine,2006,33(5):11-14.
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