反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

丁酸梭菌对犊牛生长性能和血清生化、抗氧化、免疫指标及粪便微生物数量的影响

  • 何家俊 ,
  • 吴汉葵 ,
  • 杨昕涧 ,
  • 李莉 ,
  • 赵国强 ,
  • 孟庆翔 ,
  • 解祥学
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  • 1. 广东溢多利生物科技股份有限公司, 珠海 519060;
    2. 广东燕塘乳业股份有限公司, 广州 510507;
    3. 中国农业大学动物科技学院, 北京 100193
何家俊(1993-),男,湖北襄阳人,硕士研究生,从事反刍动物营养研究。E-mail:1026760975@qq.com

收稿日期: 2021-02-20

  网络出版日期: 2021-09-18

基金资助

农业部公益性行业(农业)专项(201503134)

Effects of Clostridium butyricum on Growth Performance, Serum Biochemical, Antioxidant, Immune indexes and Fecal Microorganism Number of Calves

  • HE Jiajun ,
  • WU Hankui ,
  • YANG Xinjian ,
  • LI Li ,
  • ZHAO Guoqiang ,
  • MENG Qingxiang ,
  • XIE Xiangxue
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  • 1. Guangdong VTR Bio-Tech Co., Ltd., Zhuhai 519060, China;
    2. Guangdong Yangtang Dairy Company, Guangzhou 510507, China;
    3. College of Animal and Technology, China Agricultural University, Beijing 100193, China

Received date: 2021-02-20

  Online published: 2021-09-18

Supported by

 

摘要

本试验旨在研究饲粮中添加丁酸梭菌(Clostridium butyricum)对犊牛生长性能和血清生化、抗氧化、免疫指标及粪便微生物数量的影响。试验选取45头体重相近的(30±3)日龄海福特牛×日本黑牛♀杂交一代犊牛,随机分为3组,每组15头牛。对照组饲喂基础饲粮,试验组分别在基础饲粮的基础上添加4(CB4组)和8 g/d丁酸梭菌(CB8组)。丁酸梭菌活菌数为5×108 CFU/g。试验期30 d。结果表明:1)CB4组和CB8组的平均日增重较对照组分别提高了90和120 g(P>0.05),腹泻率较对照组分别降低了0.20和0.52个百分点。2)CB4组和CB8组的血清总蛋白(TP)含量及谷丙转氨酶(ALT)和谷草转氨酶(AST)活性较对照组显著升高(P<0.05)。CB8组的血清葡萄糖(GLU)含量较对照组和CB4组显著升高(P<0.05)。3)CB8组的血清谷胱甘肽过氧化物酶(GSH-Px)活性较对照组显著升高(P<0.05),CB4组血清超氧化物歧化酶(SOD)活性较对照组显著升高(P<0.05)。CB8组的血清GSH-Px活性较CB4组显著升高(P<0.05)。CB8组的血清免疫球蛋白G(IgG)含量较对照组显著升高(P<0.05)。4)CB4组和CB8组的粪便丁酸梭菌数量较对照组显著升高(P<0.05)。CB4组和CB8组的粪便大肠杆菌数量较对照组显著降低(P<0.05)。CB4组的粪便沙门氏菌数量较对照组显著降低(P<0.05)。由此可见,饲粮中添加丁酸梭菌能提高犊牛的生长性能,降低腹泻率,提高血清GLU和IgG含量,增强机体抗氧化能力和免疫功能,改善犊牛健康。本试验中,饲粮中添加8 g/d丁酸梭菌(活菌数为5×108 CFU/g)的效果优于4 g/d。

本文引用格式

何家俊 , 吴汉葵 , 杨昕涧 , 李莉 , 赵国强 , 孟庆翔 , 解祥学 . 丁酸梭菌对犊牛生长性能和血清生化、抗氧化、免疫指标及粪便微生物数量的影响[J]. 动物营养学报, 2021 , 33(9) : 5076 -5085 . DOI: 10.3969/j.issn.1006-267x.2021.09.028

Abstract

This experiment was conducted to investigate the effects of Clostridium butyricum on growth performance, serum biochemical, antioxidant, immune indexes and fecal microorganism number of calves. Forty-five Haifute×Japanese Black ♀ F1 hybrid calves aged about (30±3) days with similar body weight were randomly assigned to 3 groups with 15 calves per group. Calves in the control group were fed a basal diet, and others in experimental groups were fed the basal diet supplemented with 4 (CB4 group) and 8 g/d Clostridium butyricum (CB8 group), respectively. The live bacteria number of Clostridium butyricum was 5×108 CFU/g. The experiment lasted for 30 days. The results showed as follows:1) compared with the control group, the average daily gain of CB4 group and CB8 group was increased by 90 and 120 g, respectively (P>0.05), and the diarrhea rate was decreased by 0.20 and 0.52 percentage point, respectively. 2) The total protein (TP) content and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in serum of CB4 group and CB8 group were significantly higher than those of the control group (P<0.05). The serum glucose (GLU) content of CB8 group was significantly higher than that of control group and CB4 group (P<0.05). 3) The serum glutathione peroxidase (GSH-Px) activity of CB8 group was significantly higher than that of the control group (P<0.05), and the serum superoxide dismutase (SOD) activity of CB4 group was significantly higher than that of the control group (P<0.05). The serum GSH-Px activity of CB8 group was significantly higher than that of CB4 group (P<0.05). The serum immunoglobulin G (IgG) content of CB8 group was significantly higher than that of the control group (P<0.05). 4) The fecal Clostridium butyricum number of CB4 group and CB8 group was significantly higher than that of the control group (P<0.05). The fecal Escherichia coli number of CB4 group and CB8 group was significantly lower than that of the control group (P<0.05). The fecal Salmonella number of CB4 group was significantly lower than that of the control group (P<0.05). In conclusion, adding Clostridium butyricum into the diet can improve the growth performance of calves, reduce the diarrhea rate, increase the contents of GLU and IgG in serum, enhance the body antioxidant capacity and immune function, improve the health of calves. In this experiment, the effect of adding 8 g/d Clostridium butyricum is better than that of 4 g/d.

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