动物营养学报 >

2021 , Vol. 33 >Issue 9: 5162 - 5173

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.09.036

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

补喂胍基乙酸对伊犁马运动性能、糖代谢以及抗氧化能力的影响

  • 刘律 ,
  • 李晓斌 ,
  • 马艳 ,
  • 杨昊 ,
  • 张文杰 ,
  • 鲁豪 ,
  • 卫鑫岚 ,
  • 高俊洁 ,
  • 欧阳文 ,
  • 杨开伦
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  • 1. 新疆农业大学动物科学学院, 新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052;
    2. 新疆伊犁哈萨克自治州昭苏县种马场, 昭苏 835000
刘律(1994-),女,山东滨州人,硕士研究生,研究方向动物营养与饲料科学。E-mail:2367474907@qq.com

收稿日期: 2021-02-25

  网络出版日期: 2021-09-18

基金资助

自治区"十三五"重大专项"运动马健康养殖共性技术研发"（2017A01002-2-01）

收起

Effects of Supplementary Feeding Guanidinoacetic Acid on Movement Performance, Glucose Metabolism and Antioxidant Capacity of Yili Horses

  • LIU Lyu ,
  • LI Xiaobin ,
  • MA Yan ,
  • YANG Hao ,
  • ZHANG Wenjie ,
  • LU Hao ,
  • WEI Xinlan ,
  • GAO Junjie ,
  • OUYANG Wen ,
  • YANG Kailun
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  • 1. Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Xinjiang Agricultural University, Urumqi 830052, China;
    2. Yili Kazak Autonomous Prefecture of Zhaosu Racecourse, Zhaosu 835000, China

Received date: 2021-02-25

  Online published: 2021-09-18

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摘要

本试验旨在探究补喂胍基乙酸（GAA）对伊犁马运动性能、糖代谢以及抗氧化能力的影响。选取20匹年龄为2~3岁、体重[（373.20±30.53）kg]相近且经过严格训练的速度赛用伊犁马，随机分为4组，每组5匹（3♀+2），每日进行等强度训练。每匹马每天饲喂精料补充料4 kg，自由采食青干草，在此基础上，各组每天每匹马分别补喂0（CON组）、25（25 GAA组）、50（50 GAA组）和75 mg/kg BW的GAA（75 GAA组），进行为期8周的试验。在试验第47天进行2 000 m速度赛，并采集血浆和尿液样品。结果表明：1）补喂GAA可以缩短伊犁马2 000 m比赛用时。2）50 GAA组赛后即刻血浆GAA、肌酸、肌酐含量显著高于CON组（P<0.05）；50 GAA和75 GAA组赛后即刻血浆肌酸激酶活性均极显著高于CON组（P<0.01），同时50 GAA组极显著高于75 GAA组（P<0.01）。各试验组赛后即刻尿的肌酐排出量均极显著高于CON组（P<0.01）。3）各试验组赛前2 h、赛后即刻和赛后2 h血浆胰岛素含量均极显著高于CON组（P<0.01）；各试验组赛前2 h、赛后即刻和赛后2 h的血浆葡萄糖、乳酸含量均显著低于CON组（P<0.05）。4）与CON组相比，各试验组赛前2 h和赛后2 h血浆总抗氧化能力及谷胱甘肽过氧化物酶、超氧化物歧化酶活性均显著高于CON组（P<0.05）。在本试验条件下，补喂GAA提高了伊犁马2 000 m速度赛的运动成绩、血浆肌酸代谢物含量以及机体抗氧化能力，促进了运动马机体糖代谢对运动的供能。因此，GAA可作为营养调控剂，用于缓解运动马运动疲劳或促进运动后的疲劳恢复，以每匹马补喂50 mg/kg时效果最佳。

关键词： 伊犁马; 胍基乙酸; 肌酸; 糖代谢; 抗氧化能力

本文引用格式

刘律 , 李晓斌 , 马艳 , 杨昊 , 张文杰 , 鲁豪 , 卫鑫岚 , 高俊洁 , 欧阳文 , 杨开伦 . 补喂胍基乙酸对伊犁马运动性能、糖代谢以及抗氧化能力的影响[J]. 动物营养学报, 2021 , 33(9) : 5162 -5173 . DOI: 10.3969/j.issn.1006-267x.2021.09.036

Abstract

The purpose of this study was to investigate the effects of supplementary feeding guanidineacetic acid (GAA) on movement performance, glucose metabolism and antioxidant capacity of Yili horses. Twenty Yili horses aged 2 to 3 years old, similar in weight (373.20±30.53) kg, and rigorously trained for speed races, were randomly divided into 4 groups and with 5 horses (3♀+2) in each group, and the training intensity were consistent. Each horse was fed 4 kg of concentrate supplements per day, and freely collected dry herbage. On this basis, each horse in each group was fed 0 (CON group), 25 (25 GAA group), 50 (50 GAA group) and 75 mg/kg GAA (75 GAA group) every day for an 8-week trial. On the 47th day of the trial, a 2 000 m speed race was performed, and blood and urine samples were collected. The results showed as follows:1) supplementation of GAA acid can shorten the time of 2 000 m race in Yili horses. 2) The contents of plasma GAA, creatine and creatinine in 50 GAA group were significantly higher than those in CON group immediately after competition (P<0.05). Plasma creatine kinase activity in 50 GAA and 75 GAA groups was significantly higher than that in CON group immediately after competition (P<0.01). The urine creatinine excretion in all experimental groups was significantly higher than that in CON group (P<0.01). 3) The plasma insulin content in each experimental group was significantly higher than that in CON group at 2 h before, immediately and 2 h after competition (P<0.05). The contents of plasma glucose and lactic acid in each experimental group at 2 h before, immediately and 2 h after the competition were significantly lower than those in the CON group (P<0.05). 4) The activities of plasma total antioxidant capacity, glutathione peroxidase and superoxide dismutase in all experimental groups were significantly higher than those in CON group at 2 h before and 2 h after competition (P<0.05). Under the conditions of this experiment, GAA supplementation increase the content of plasma creatine metabolites, the ability of antioxidation and the performance of 2000 m speed race, and promote the energy supply of glucose metabolism to exercise. Therefore, GAA can be used as a nutritional regulator to relieve exercise fatigue or promote fatigue recovery of sports horses. The best supplementation level of GAA is 50 mg/kg per horse.

Key words： Yili horses; GAA; creatine; glucose metabolism; antioxidant capacity

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