动物营养学报 >

2021 , Vol. 33 >Issue 9: 5266 - 5276

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.09.046

实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

玉米赤霉烯酮降解菌株的筛选及降解特性研究

  • 段锦 ,
  • 丁轲 ,
  • 余祖华 ,
  • 李旺 ,
  • 李元晓 ,
  • 何万领 ,
  • 曹平华 ,
  • 赵龙妹 ,
  • 贾艳艳 ,
  • 王玉琴 ,
  • 刘宁
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  • 1. 河南科技大学宏翔生物饲料实验室, 洛阳 471000;
    2. 河南省动物疫病与公共卫生重点实验室, 洛阳 471000;
    3. 河南省肉羊繁育工程技术研究中心, 洛阳 471000
段锦(1993-),女,河南永城人,硕士研究生,从事动物微生态与动物传染病学研究。E-mail:dj1108mm@163.com

收稿日期: 2021-01-24

  网络出版日期: 2021-09-18

基金资助

国家自然科学基金项目（32072771）；河南省自然科学基金项目（182300410052）

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Screening and Degradation Characteristics of Strains Degrading Zearalenone

  • DUAN Jin ,
  • DING Ke ,
  • YU Zuhua ,
  • LI Wang ,
  • LI Yuanxiao ,
  • HE Wanling ,
  • CAO Pinghua ,
  • ZHAO Longmei ,
  • JIA Yanyan ,
  • WANG Yuqin ,
  • LIU Ning
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  • 1. Hongxiang Biological Feed Laboratory, Henan University of Science and Technology, Luoyang 471000, China;
    2. Key Laboratory of Animal Disease and Public Health of Henan Province, Luoyang 471000, China;
    3. Research Center of Breeding Engineering Technology for Meat Sheep of Henan Province, Luoyang 471000, China

Received date: 2021-01-24

  Online published: 2021-09-18

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摘要

本试验旨在筛选能够有效降解玉米赤霉烯酮（ZEN）的微生物菌株。试验以ZEN为唯一碳源对发霉饲料、动物粪便等样品中的菌株进行分离筛选，选取其中降解率较高的菌株进行鉴定。研究菌株不同活性成分的ZEN降解效率以及不同培养条件下菌株的ZEN降解效率，初步探明菌株降解机制，优化菌株降解条件，并通过小鼠试验评价菌株的体内ZEN降解效果。结果表明：从样品中筛选出2株能有效降解ZEN的菌株，分别命名为NA-J21和MLS-H32，经鉴定分别是解淀粉芽孢杆菌（Bacillus amyloliquefaciens）及枯草芽孢杆菌（Bacillus subtilis）。37℃培养48 h，菌株NA-J21与MLS-H32的ZEN降解率分别为71.3%和61.5%。2株菌主要通过细胞分泌的胞外酶降解ZEN，此外菌株细胞壁对ZEN有一定的吸附能力。菌株NA-J21降解ZEN的最适条件为接种量2.0%，初始pH 7.0，温度37℃，培养时间12 h；菌株MLS-H32的最佳ZEN降解条件为接种量2.5%，初始pH 7.0，温度32℃，培养时间12 h。菌株NA-J21和MLS-H32能够缓解ZEN中毒引起的小鼠脏器损伤，对ZEN污染小鼠有一定治疗效果。综上所述，本试验筛选的菌株NA-J21和MLS-H32能够有效降解ZEN，是微生物降解ZEN的良好选择。

关键词： 霉菌毒素; 玉米赤霉烯酮; 微生物降解; 芽孢杆菌; 降解特性

本文引用格式

段锦 , 丁轲 , 余祖华 , 李旺 , 李元晓 , 何万领 , 曹平华 , 赵龙妹 , 贾艳艳 , 王玉琴 , 刘宁 . 玉米赤霉烯酮降解菌株的筛选及降解特性研究[J]. 动物营养学报, 2021 , 33(9) : 5266 -5276 . DOI: 10.3969/j.issn.1006-267x.2021.09.046

Abstract

The purpose of this study was to screen the microorganism strains that could safely and effectively degrade zearalenone (ZEN). ZEN was used as the sole carbon to isolate and screen the strains in moldy feed, animal feces and other samples, the strains with higher degradation rates were selected out for identification. The ZEN degradation mechanism of the strains was preliminarily explored. The effects of different inoculation amount, temperature, pH and culture time on the degradation rate were studied, and the safety of the strain was evaluated by mouse experiments. The results showed that two strains that can effectively degraded ZEN were selected from the samples. They were identified as Bacillus amyloliquefaciens and Bacillus subtilis, respectively. After cultured 48 h, 71.3% of the ZEN was degraded by the NA-J21 strain, and 61.5% of ZEN was degraded by the MLS-H32 strain. Both strains degrade ZEN mainly through extracellular enzymes secreted by cells, and the cell walls of the strains had a certain degree of adsorption capacity for ZEN. The optimal conditions for NA-J21 strain to degrade ZEN were that the inoculation amount was 2.0%, the initial pH was 7.0, at a temperature of 37℃ and culture time was 12 h; and for MLS-H32, the optimal conditions for ZEN degradation were that the inoculation amount was 2.5%, the initial pH was 7.0, 32℃, and culture time was 14 h. NA-J21 and MLS-H32 strains could alleviate the organ damage caused by ZEN poisoning in mice, and were safe on some level. The Bacillus amyloliquefaciens and Bacillus subtilis screened in this study can effectively degrade ZEN and both are good microbial for the degradation of ZEN.

Key words： mycotoxins; zearalenone; bio-degradation; Bacillus; degradation characteristics

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