动物营养学报 >

2022 , Vol. 34 >Issue 1: 215 - 227

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.021

研究论文 RESEARCH PAPER

黄芩素对22~63日龄湘黄肉鸡生长性能、免疫功能、抗氧化能力、血脂状态及肉品质的影响

  • 高书锋 ,
  • 雷平 ,
  • 李咏梅 ,
  • 王升平 ,
  • 龚平 ,
  • 曾奥 ,
  • 曾发姣 ,
  • 舒燕 ,
  • 周小玲 ,
  • 周映华 ,
  • 缪东 ,
  • 刘惠知
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  • 湖南省微生物研究院, 饲用微生态制剂湖南省工程实验室, 湖南省农用微生物工程技术研究中心, 长沙 410009
高书锋(1978-),男,河南洛阳人,副研究员,硕士,主要从事动物营养与健康养殖研究。E-mail:oooobook@163.com

收稿日期: 2021-05-31

  网络出版日期: 2022-01-18

基金资助

国家自然科学基金项目(32100069);湖南省自然科学基金项目(2017JJ2166,2020JJ5322)

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Effects of Baicalein on Growth Performance, Immune Function, Antioxidant Ability, Blood Lipid Status and Meat Quality of Hunan Yellow Chickens during 22 to 63 Days of Age

  • GAO Shufeng ,
  • LEI Ping ,
  • LI Yongmei ,
  • WANG Shengping ,
  • GONG Ping ,
  • ZENG Ao ,
  • ZENG Fajiao ,
  • SHU Yan ,
  • ZHOU Xiaoling ,
  • ZHOU Yinghua ,
  • MIAO Dong ,
  • LIU Huizhi
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  • Hunan Provincial Engineering and Technology Research Center for Agricultural Microbiology Application, Feeding Probiotics Engineering Laboratory of Hunan Province, Hunan Institute of Microbiology, Changsha 410009, China

Received date: 2021-05-31

  Online published: 2022-01-18

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摘要

本试验旨在研究黄芩素对22~63日龄湘黄肉鸡生长性能、免疫功能、抗氧化能力、血脂状态及肉品质的影响。选择200只初始体重相近的健康22日龄湘黄肉鸡，随机分为5个组，每组5个重复，每个重复8只鸡（公母各占1/2）。对照组饲喂基础饲粮，试验组在基础饲粮基础上分别添加0.10、0.20、0.30和0.40 g/kg黄芩素。试验期42 d。结果表明：1）与对照组相比，22~42日龄，0.20 g/kg黄芩素组平均日采食量（ADFI）显著降低（P<0.05），0.30 g/kg黄芩素组ADFI显著增加（P<0.05），各试验组平均日增重（ADG）、料重比（F/G）和死亡率均无显著差异（P>0.05）；43~63日龄，各试验组ADFI均显著降低（P<0.05），而ADG、F/G和死亡率均无显著差异（P>0.05）；22~63日龄，0.20 g/kg黄芩素组ADFI和F/G均显著降低（P<0.05），0.40 g/kg黄芩素组ADG显著降低（P<0.05），各试验组死亡率均无显著差异（P>0.05）。2）与对照组相比，0.20 g/kg黄芩素组血清免疫球蛋白A（IgA）含量显著增加（P<0.05），0.30 g/kg黄芩素组血清IgA和免疫球蛋白G（IgG）含量均显著增加（P<0.05）；各试验组血清白细胞介素-2（IL-2）含量均显著增加（P<0.05），而血清免疫球蛋白M（IgM）、白细胞介素-1β（IL-1β）、白细胞介素-4（IL-4）和肿瘤坏死因子-α（TNF-α）含量均无显著差异（P>0.05）。3）与对照组相比，0.10和0.20 g/kg黄芩素组血清总超氧化物歧化酶（T-SOD）活性均显著增加（P<0.05），各试验组血清总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）活性及谷胱甘肽（GSH）、过氧化氢（H2O2）和丙二醛（MDA）含量均无显著差异（P>0.05）；0.20 g/kg黄芩素组肝脏T-AOC和T-SOD活性均显著增加（P<0.05），0.10 g/kg黄芩素组肝脏T-AOC显著增加（P<0.05），各试验组肝脏H2O2含量均显著降低（P<0.05），而肝脏GSH-Px活性、GSH和MDA含量均无显著差异（P>0.05）。4）与对照组相比，0.30 g/kg黄芩素组血清总胆固醇（TC）含量显著降低（P<0.05）；各试验组血清甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）含量均无显著差异（P>0.05）。5）与对照组相比，0.40 g/kg黄芩素组胸肌屠宰后24 h黄度值显著降低（P<0.05）；各试验组胸肌屠宰后24 h亮度值均显著降低（P<0.05），而屠宰后45 min pH与亮度、红度、黄度值以及屠宰后24 h pH、红度值、剪切力和滴水损失率均无显著差异（P>0.05）。由此可见，黄芩素可改善22~63日龄湘黄肉鸡的生长性能、免疫功能、抗氧化能力、血脂状态和肉品质，综合考虑，饲粮中黄芩素的适宜添加量为0.20~0.30 g/kg。

关键词： 黄芩素; 湘黄肉鸡; 生长性能; 免疫功能; 抗氧化能力; 血脂状态; 肉品质

本文引用格式

高书锋 , 雷平 , 李咏梅 , 王升平 , 龚平 , 曾奥 , 曾发姣 , 舒燕 , 周小玲 , 周映华 , 缪东 , 刘惠知 . 黄芩素对22~63日龄湘黄肉鸡生长性能、免疫功能、抗氧化能力、血脂状态及肉品质的影响[J]. 动物营养学报, 2022 , 34(1) : 215 -227 . DOI: 10.3969/j.issn.1006-267x.2022.01.021

Abstract

This experiment was to investigate the effects of baicalein on growth performance, immune function, antioxidant ability, blood lipid status and meat quality of Hunan yellow chickens during 22 to 63 days of age. Two hundred healthy 22-day-old Hunan yellow chickens with similar initial body weight were randomly allocated into 5 groups with 5 replicates per group and 8 chickens per replicate (males and females in half). Chickens in control group were fed a basal diet, and those in experiment groups were fed the basal diet supplemented with 0.10, 0.20, 0.30 and 0.40 g/kg baicalein, respectively. The experiment lasted for 42 days. The results showed as follows:1) compared with the control group, for 22 to 42 days of age,the average daily feed intake (ADFI) of 0.20 and 0.30 g/kg baicalein groups were significant1y decreased and increased, respectively (P<0.05), but no significant difference was observed in the average daily gain (ADG), feed/gain (F/G) and mortality of all experimental groups (P>0.05); for 43 to 63 days of age, the ADFI of all experiment groups were significantly decreased (P<0.05), while the ADG, F/G and mortality had no significant differences (P>0.05); for 22 to 63 days of age, the ADFI and F/G of 0.20 g/kg baicalein group and the ADG of 0.40 g/kg baicalein group were significantly decreased (P<0.05), but no significant difference was observed in the mortality of all experiment groups (P>0.05). 2) Compared with the control group, the immunoglobulin A (IgA) content in serum of 0.20 g/kg baicalein group was significantly increased (P<0.05), and the IgA and immunoglobulin G (IgG) contents in serum of 0.30 g/kg baicalein group were significantly increased (P<0.05); the interleukin-2 (IL-2) content in serum of all experiment groups was significantly increased (P<0.05), while the IgM, interleukin-1β (IL-1β), interleukin-4 (IL-4) and tumor necrosis factor-α (TNF-α) contents had no significant differences (P>0.05). 3) Compared with the control group, significant increases of total superoxide dismutase (T-SOD) activity in serum were observed in 0.10 and 0.20 g/kg baicalein groups (P<0.05), and no significant difference was detected in total antioxidant capacity (T-AOC), glutathione peroxidase(GSH-Px) activity, glutathione (GSH), hydrogen peroxide(H2O2) and malondialdehyde(MDA) contents in serum of all experiment groups (P>0.05); the liver T-AOC and T-SOD activity of 0.20 g/kg baicalein group were increased significantly (P<0.05), the liver T-AOC of 0.10 g/kg baicalein group was significantly increased (P<0.05), the liver H2O2 content of all experiment groups was significantly decreased (P<0.05), while the liver GSH-Px activity, GSH and MDA contents had no significant differences (P>0.05). 4) Compared with the control group, serum total cholesterol (TC) content of 0.30 g/kg baicalein group was decreased (P<0.05), but no significant differences were observed in triglyceride (TG), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) contents in serum of all experimental groups (P>0.05). 5) Compared with the control group, the yellowness of chest muscle for 24 h after slaughter of 0.40 g/kg baicalein group was significantly decreased (P<0.05); the lightness of chest muscle for 24 h after slaughter of all experiment groups was significantly decreased (P<0.05), while the pH, lightness, redness and yellowness for 45 min after slaughter, pH and redness for 24 h after slaughter, shear force and drip loss rate had no significant differences (P>0.05). In conclusion, baicalein can improve the growth performance, immune function, antioxidant ability, blood lipid status and meat quality of Hunan yellow chickens during 22 to 63 days of age. Considering all results of this experiment, the appropriate baicalein supplemental levels in the diet are 0.20 to 0.30 g/kg.

Key words： baicalein; Hunan yellow chickens; growth performance; immune function; antioxidant ability; blood lipid status; meat quality

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