动物营养学报 >

2022 , Vol. 34 >Issue 1: 228 - 236

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.022

研究论文 RESEARCH PAPER

饲粮添加不同水平的维生素A对肉鸡免疫功能及肝脏、十二指肠组织中一氧化氮代谢的影响

  • 李胤豪 ,
  • 张清月 ,
  • 郭晓宇 ,
  • 闫素梅 ,
  • 史彬林 ,
  • 赵艳丽
展开
  • 内蒙古农业大学动物科学学院, 动物营养与饲料科学自治区高等学校重点实验室, 呼和浩特 010018
李胤豪(1991-),男,甘肃张掖人,博士研究生,从事动物营养学的研究。E-mail:29749409@qq.com

收稿日期: 2021-05-25

  网络出版日期: 2022-01-18

基金资助

内蒙古自然科学基金项目(2015BS0315);内蒙古自治区高等学校科学研究项目(NJZZ16056)

收起

Effects of Dietary Supplementation with Different Levels of Vitamin A on Immune Function and Metabolism of Nitric Oxide in Liver and Duodenum of Broilers

  • LI Yinhao ,
  • ZHANG Qingyue ,
  • GUO Xiaoyu ,
  • YAN Sumei ,
  • SHI Binlin ,
  • ZHAO Yanli
Expand
  • Inner Mongolia Autonomous Region Key Laboratory of Animal Nutrition and Feed Science, Collage of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2021-05-25

  Online published: 2022-01-18

Fold

摘要

本试验旨在研究饲粮中添加不同水平的维生素A对肉鸡免疫功能及肝脏、十二指肠组织中一氧化氮（NO）含量及诱导型一氧化氮合酶（iNOS）基因相对表达量的影响。采用单因子完全随机试验设计，选取1日龄爱拔益加（AA）肉鸡240只（公母各占1/2），随机分为5组，每组6个重复，每个重复8只。各组分别在基础饲粮中添加不同水平的维生素A，添加水平依次为3 000、6 000、15 000、30 000和45 000 IU/kg。试验期42 d。结果表明：1）饲粮维生素A水平对21和42日龄肉鸡法氏囊指数均存在趋于显著的影响（P=0.078，P=0.060），以6 000和15 000 IU/kg组法氏囊指数最高，45 000 IU/kg组最低。2）饲粮维生素A水平对21和42日龄肉鸡血清中白细胞介素-1（IL-1）、42日龄白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）含量有显著影响（P<0.05），且随维生素A水平的提高均呈显著的二次曲线降低的剂量依赖关系（P<0.05），各组均以15 000 IU/kg组含量较高，45 000 IU/kg组含量较低；饲粮维生素A水平对42日龄肉鸡血清中白细胞介素-2（IL-2）含量有趋于显著的影响（P=0.075），且随饲粮维生素A水平的提高呈显著的一次线性升高的剂量依赖关系（P<0.05）。3）随饲粮维生素A水平的增加，肝脏NO含量呈显著的二次曲线下降（P<0.05），以6 000 IU/kg组的含量最高；肝脏iNOS、总一氧化氮合酶（tNOS）活性以及iNOS基因相对表达量均呈显著的一次降低趋势（P<0.05），30 000和45 000 IU/kg组酶活性及基因相对量表达量显著低于其他组（P<0.05）。十二指肠组织中NO含量、iNOS、tNOS活性以及iNOS基因相对表达量变化规律与之相似。综上所述，饲粮添加6 000~15 000 IU/kg的维生素A促进了肉鸡的免疫功能。肉鸡肝脏和十二指肠组织中NO含量和iNOS、tNOS活性及iNOS基因相对表达量与饲粮维生素A添加水平呈剂量依赖关系，低剂量提高NO含量，上调iNOS基因相对表达量，而高剂量则出现抑制作用。

关键词： 维生素A; 肉鸡; 免疫功能; NO; iNOS基因

本文引用格式

李胤豪 , 张清月 , 郭晓宇 , 闫素梅 , 史彬林 , 赵艳丽 . 饲粮添加不同水平的维生素A对肉鸡免疫功能及肝脏、十二指肠组织中一氧化氮代谢的影响[J]. 动物营养学报, 2022 , 34(1) : 228 -236 . DOI: 10.3969/j.issn.1006-267x.2022.01.022

Abstract

In this study, the effects of dietary vitamin A supplementation on immune function of broilers were studied, and the content of nitric oxide (NO) in the liver and duodenum and the relative expression level of inducible nitric oxide synthase (iNOS) mRNA were also tested. A total of 240 one-day-old Arbor Acres (AA) broiler chickens (half male and half female) were randomly allotted into five groups with six replicates of eight broilers in each replicate. The trial lasted for 42 days. Five different levels of vitamin A were added to basic diets in each group, with levels of 3 000, 6 000, 15 000, 30 000 and 45 000 IU/kg vitamin A, respectively. The results showed as follows:1) the dietary vitamin A had a significant effect on the bursa of Fabricius index of broilers at 21 and 42 days of age (P=0.078,P=0.060). The bursa of Fabricius index of the 6 000 and 15 000 IU/kg groups was the highest, and the 45 000 IU/kg group was the lowest. 2) Vitamin A levels had a significant effect on the serum contents of interleukin-1 (IL-1) of broiler at 21 and 42 days of age and interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) of broiler at 42 days of age (P<0.05), and with the increase of vitamin A level, there was a significant quadratic curve decreasing dose-dependent relationship (P<0.05), and the content was higher in the 15 000 IU/kg group and lower in the 45 000 IU/kg group; At 42 days of age, the level of dietary vitamin A tended to have a significant effect on the serum interleukin-2 content of broilers (P=0.075), and there was a significant linear increase with the increase of dietary vitamin A level (P<0.05). 3) With the increase of dietary vitamin A level, the liver nitric oxide content showed a significant quadratic curve decrease (P<0.05), and 6 000 IU/kg group was the highest; iNOS, total nitric oxide synthase (tNOS) activity and iNOS mRNA relative expression level showed a significant linear decrease (P<0.05). The enzyme activity and gene expression of the 30 000 and 45 000 IU/kg groups were significantly lower than those of the other groups (P<0.05).The changes of NO content, iNOS, tNOS activities and iNOS mRNA relative expression level in the duodenum were similar to those in the liver. In summary, the dietary supplementation with vitamin A of 6 000 to 15 000 IU/kg promotes the development of the immune function of the broilers. The NO content, iNOS, tNOS activities and iNOS mRNA relative expression level in the liver and duodenum of broilers have a dose effect with vitamin A, which is low level increases NO content and up-regulates the iNOS mRNA relative expression level, while high level shows inhibitory effect.

Key words： vitamin A; broiler; immune function; NO; iNOS gene

参考文献

[1] 石惠宇,闫素梅.维生素A对动物氧化应激的减缓作用机制[J].动物营养学报,2019,31(6):2458-2464. SHI H Y,YAN S M.Mitigation mechanism of vitamin A on oxidative stress in animals[J].Chinese Journal of Animal Nutrition,2019,31(6):2458-2464.(in Chinese)
[2] 郭晓宇,韩俊英,闫素梅,等.维生素A对肉鸡生长性能、免疫及抗氧化功能的影响[J].动物营养学报,2019,31(8):3582-3589. GUO X Y,HAN J Y,YAN S M,et al.Effects of vitamin A on growth performance,immunity and antioxidant function of broilers[J].Chinese Journal of Animal Nutrition,2019,31(8):3582-3589.(in Chinese)
[3] SAVARIS V D L,SOUZA C,WACHHOLZ L,et al.Interactions between lipid source and vitamin A on broiler performance,blood parameters,fat and protein deposition rate,and bone development[J].Poultry Science,2021,100(1):174-185.  
[4] SCHMIDT H H,WALTER U.NO at work[J].Cell,1994,78(6):919-925.  
[5] LI J,SHI B,YAN S,et al.Effects of chitosan on nitric oxide production and inducible nitric oxide synthase activity and mRNA expression in weaned piglets[J].Czech Journal of Animal Science,2015,60(8):359-366.
[6] ZHENG L,PAN Y Y,FENG Y H,et al.L-arginine supplementation in mice enhances No production in spleen cells and inhibits Plasmodium yoelii transmission in mosquitoes[J].Parasites & Vectors,2015,8:326.
[7] HODA K N,MOHAMMAD H S,GHOLAMREZA E D.An in vitro model for investigation of vitamin A effects on wound healing[J].International Journal for Vitamin and Nutrition Research,2021(1):1-6.
[8] SHI H Y,YAN S M,GUO Y M,et al.Vitamin A pretreatment protects NO-induced bovine mammary epithelial cells from oxidative stress by modulating Nrf2 and NF-κB signaling pathways[J].Journal of Animal Science,2018,96(4):1305-1316.  
[9] GOVERSE G,OLIVIER B J,MOLENAAR R,et al.Vitamin A metabolism and mucosal immune function are distinct between BALB/c and C57BL/6 mice[J].European Journal of Immunology,2015,45(1):89-100.  
[10] 郭世伟.黄花蒿水提物对肉仔鸡生长性能及免疫和抗氧化功能的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2020. GUO S W.Effects of Artemisia annua L. aqueous extract on growth performance,immune and antioxidative function of broilers[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University,2020.(in Chinese)
[11] HUNG L F,LAI J H,LIN L C,et al.Retinoid acid inhibits IL-1-induced iNOS,COX-2 and chemokine production in human chondrocytes[J].Immunological Investigations,2008,37(7):675-693.  
[12] WANG Y B,LI L,GOU Z Y,et al.Effects of maternal and dietary vitamin A on growth performance,meat quality,antioxidant status,and immune function of offspring broilers[J].Poultry Science,2020,99(8):3930-3940.  
[13] ROSS A C.Vitamin A and retinoic acid in T cell-related immunity[J].American Journal of Clinical Nutrition,2012,96(5):1166S-1172S.
[14] YANG H M,LIANG J R,DAI H,et al.Effects of vitamin A supplementation in the diet of breeding geese on offspring intestinal tissue morphology and immune performance[J].Asian Australasian Journal of Animal Sciences,2020,33(9):1463-1469.  
[15] 梁静茹,代航,杨海明,等.饲粮添加维生素A对种公鹅肝脏维生素A沉积、免疫及抗氧化性能的影响[J].中国饲料,2020(9):69-73. LIANG J R,DAI H,YANG H M,et al.Effects of dietary vitamin A supplementation on vitamin A content,immunity and antioxidant activity in liver of geese[J].China Feed,2020(9):69-73.(in Chinese)
[16] SALAK-JOHNSON J L,MCGLONE J J.Making sense of apparently conflicting data:stress and immunity in swine and cattle[J].Journal of Animal Science,2007,85(Suppl.13):E81-E88.
[17] MCDANIEL K L,RESTORI K H,DODDS J W,et al.Vitamin A-deficient hosts become nonsymptomatic reservoirs of Escherichia coli-like enteric infections[J].Infection and Immunity,2015,83(7):2984-2991.  
[18] BONO M R,TEJON G,FLORES-SANTIBAÑEZ F,et al.Retinoic acid as a modulator of T cell immunity[J].Nutrients,2016,8(6):349.
[19] YUAN J M,ROSHDY A R,GUO Y M,et al.Effect of dietary vitamin A on reproductive performance and immune response of broiler breeders[J].PLoS One,2014,9(8):e105677.
[20] MONCADA S,HIGGS E A.The discovery of nitric oxide and its role in vascular biology[J].British Journal of Pharmacology,2006,147(Suppl.1):S193-S201.
[21] UǦAR-CANKAL D,OZMERIC N.A multifaceted molecule,nitric oxide in oral and periodontal diseases[J].Clinica Chimica Acta,2006,366(1/2):90-100.
[22] GROSS T J,KREMENS K,POWERS L S,et al.Epigenetic silencing of the human NOS2 gene:rethinking the role of nitric oxide in human macrophage inflammatory responses[J].Journal of Immunology,2014,192(5):2326-2338.  
[23] OKADA H,OHTSUKA H,KON NAI S,et al.Effects of lipopolysaccharide on production of interleukin-1 and interleukin-6 by bovine mammary epithelial cells in vitro[J].The Journal of Veterinary Medical Science,1999,61(1):33-35.  
[24] DHEEN S T,JUN Y,YAN Z,et al.Retinoic acid inhibits expression of TNF-α and iNOS in activated rat microglia[J].Glia,2005,50(1):21-31.  
[25] NEGHAB H K,SOHEILIFAR M H,DJAVID G E.An in vitro model for investigation of vitamin A effects on wound healing[J].International Journal for Vitamin and Nutrition Research,2021:1-6.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2