动物营养学报 >

2022 , Vol. 34 >Issue 1: 404 - 412

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.038

研究论文 RESEARCH PAPER

不同剂量25羟基维生素D3对断奶前犊牛生长性能、营养物质表观消化率、血浆代谢物及粪便菌群的影响

  • 王丽华 ,
  • 徐宏建 ,
  • 张全宇 ,
  • 张城瑞 ,
  • 冯冠智 ,
  • 张永根
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  • 东北农业大学动物科学技术学院, 哈尔滨 150030
王丽华(1995-),女,内蒙古通辽人,硕士研究生,从事反刍动物营养与饲料科学研究。E-mail:wanglihua4822@163.com

收稿日期: 2021-06-22

  网络出版日期: 2022-01-18

基金资助

财务部和农业农村部国家现代农业产业技术体系资助

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Effects of Different Doses of 25-Hydroxyvitamin D3 on Growth Performance, Nutrient Apparent Digestibility, Plasma Metabolites and Fecal Flora of Pre-Weaning Calves

  • WANG Lihua ,
  • XU Hongjian ,
  • ZHANG Quanyu ,
  • ZHANG Chengrui ,
  • FENG Guanzhi ,
  • ZHANG Yonggen
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  • College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2021-06-22

  Online published: 2022-01-18

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摘要

本试验旨在研究饲粮添加不同剂量25羟基维生素D3[25（OH）VD3]对断奶前犊牛生长性能、营养物质表观消化率、血浆代谢物及粪便菌群的影响。选取18头健康、体重[（40.68±1.20）kg]相近的新生荷斯坦犊牛，随机分为3个组，每组6头牛。对照组饲喂基础饲粮，不添加25（OH）VD3；试验组每头犊牛每天分别在基础饲粮中添加6 000（低剂量组）和12 000 IU（高剂量组）的25（OH）VD3。试验期56 d。结果表明：1）与对照组相比，低剂量组和高剂量组体重和平均日增重显著提高（P<0.05），高剂量组体高显著提高（P<0.05），低剂量组和高剂量组粪便评分显著降低（P<0.05）。2）与对照组相比，低剂量组和高剂量组干物质表观消化率显著提高（P<0.05），高剂量组中性洗涤纤维（P=0.05）和钙表观消化率（P<0.05）显著提高。3）与对照组相比，低剂量组和高剂量组血浆25（OH）VD3和钙含量显著提高（P<0.05），高剂量组血浆磷含量显著提高（P<0.05）。4）与对照组相比，低剂量组和高剂量组血浆白细胞介素-2含量显著降低（P<0.05）。5）与对照组相比，低剂量组和高剂量组粪便中毛螺菌科（Lachnospiraceae）和普雷沃式菌科（Prevotellaceae）相对丰度显著提高（P<0.05），粪便中鼠杆菌科（Muribaculaceae）、克里斯滕森菌科（Christensenellaceae）、螺旋体科（Spirochaetaceae）和未培养_细菌_o_柔膜菌纲_RF39（uncultured_bacterium_o_Mollicutes_RF39）相对丰度显著降低（P<0.05）。由此可见，饲粮添加25（OH）VD3可以提高断奶前犊牛生长性能，促进营养物质消化吸收，降低炎症反应，改善粪便菌群，降低腹泻发生。

关键词： 犊牛; 25(OH)VD3; 生长性能; 血浆代谢物; 粪便菌群

本文引用格式

王丽华 , 徐宏建 , 张全宇 , 张城瑞 , 冯冠智 , 张永根 . 不同剂量25羟基维生素D3对断奶前犊牛生长性能、营养物质表观消化率、血浆代谢物及粪便菌群的影响[J]. 动物营养学报, 2022 , 34(1) : 404 -412 . DOI: 10.3969/j.issn.1006-267x.2022.01.038

Abstract

The objective of this experiment was to investigate the effects of dietary different doses of 25-hydroxyvitamin D3[25(OH)VD3] on growth performance, nutrient apparent digestibility, plasma metabolites and fecal flora of pre-weaning calves. Eighteen healthy newborn Holstein calves with similar body weight of (40.68±1.21) kg were randomly divided into 3 groups with 6 calves per group. Calves in the control group were fed a basal diet without 25(OH)VD3, and others in experimental groups were fed the basal diets supplemented with 6 000 (low-dose group) and 12 000 IU (high-dose group) 25(OH)VD3 per calf per day, respectively. The experiment lasted for 56 days. The results showed as follows:1) compared with the control group, the body weight and average daily gain of low-dose group and high-dose group were significantly increased (P<0.05), the body height of high-dose group was significantly increased (P<0.05), and the fecal score of low-dose group and high-dose group was significantly decreased (P<0.05). 2) Compared with the control group, the dry matter apparent digestibility of low-dose group and high-dose group was significantly increased (P<0.05), and the apparent digestibility of neutral detergent fiber (P=0.05) and calcium (P<0.05) of high-dose group was significantly increased. 3) Compared with the control group, the contents of 25(OH)VD3 and calcium in plasma of low-dose group and high-dose group were significantly increased (P<0.05), and the plasma phosphorus content of high-dose group was significantly increased (P<0.05). 4) Compared with the control group, the plasma interleukin-2 content of low-dose group and high-dose group was significantly decreased (P<0.05). 5) Compared with the control group, the relative abundances of Lachnospiraceae and Prevotellaceae in feces of low-dose group and high-dose group were significantly increased (P<0.05), and the relative abundances of Muribaculaceae, Christensenellaceae, Spirochaetaceae and uncultured_bacterium_o_Mollicutes_RF39 in feces were significantly decreased (P<0.05). In conclusion, dietary 25(OH)VD3 can improve the growth performance of pre-weaning calves, promote the digestion and absorption of nutrients, decrease the inflammatory response, improve the fecal flora, and reduce the occurrence of diarrhea.

Key words： calves; 25(OH)VD3; growth performance; plasma metabolites; fecal flora

参考文献

[1] NELSON C D,POWELL J L,PRICE D M,et al.Assessment of serum 25-hydroxyvitamin D concentrations of beef cows and calves across seasons and geographical locations[J].Journal of Animal Science,2016,94(9):3958-3965.  
[2] 陈相辉.犊牛佝偻病的防治措施分析[J].中国动物保健,2017,19(6):14-15. CHEN X H.Analysis of prevention and control measures of calf rickets[J].China Animal Health,2017,19(6):14-15.(in Chinese)
[3] TAYLOR M S,KNOWLTON K F,MCGILLIARD M L,et al.Blood mineral,hormone,and osteocalcin responses of multiparous Jersey cows to an oral dose of 25-hydroxyvitamin D3 or vitamin D3 before parturition[J].Journal of Dairy Science,2008,91(6):2408-2416.  
[4] NORMAN A W,HENRY H.1,25-dihydroxycholecalciferol-a hormonally active form of vitamin D[J].Recent Progress in Hormone Research,1974,30:431-480.
[5] TRECHSEL U,BONJOUR J P,FLEISCH H.Influence of dietary calcium,phosphorus,and vitamin D on the conversion of 25-hydroxyvitamin D3 to 1,25-dihydroxyvitamin D3 by kidney tubules of diphosphonate-treated quails[J].Calcified Tissue Research,1977,22(Suppl):461-462.
[6] 王海洲,曹金党,秦甜甜.25-羟基维生素D3的生物学特性及其在生猪饲粮中的应用研究进展[J].养猪,2021(1):45-48. WANG H Z,CAO J D,QIN T T.Biological characteristics of 25 hydroxyvitamin D3 and its application in pig diet[J].Swine Production,2021(1):45-48.(in Chinese)
[7] NELSON C D,LIPPOLIS J D,REINHARDT T A,et al.Vitamin D status of dairy cattle:outcomes of current practices in the dairy industry[J].Journal of Dairy Science,2016,99(12):10150-10160.  
[8] XU H J,WANG L H,ZHANG Q Y,et al.Effects of 25-hydroxyvitamin D3 on growth performance,fecal scores,vitamin D3 metabolites,antioxidant status,and inflammatory and stress-related parameters in weaning calves[J/OL].Animal Feed Science and Technology,2021:114946.(2021-04-22)[2021-05-12].https://www.sciencedirect.com/science/article/abs/pii/S0377840121001322.DOI:10.1016/j.anifeedsci.2021.114946.
[9] BAR A,SHARVIT M,NOFF D,et al.Absorption and excretion of cholecalciferol and of 25-hydroxycholecalciferol and metabolites in birds[J].The Journal of Nutrition,1980,110(10):1930-1934.  
[10] YANG J W,TIAN G,CHEN D W,et al.Dietary 25-hydroxyvitamin D3 supplementation alleviates porcine epidemic diarrhea virus infection by improving intestinal structure and immune response in weaned pigs[J].Animals,2019,9(9):627.
[11] 廖波,张克英,丁雪梅,等.饲粮添加25-羟基维生素D3对轮状病毒攻毒和未攻毒断奶仔猪血清和肠内容物抗体和细胞因子水平的影响[J].动物营养学报,2011,23(1):34-42. LIAO B,ZHANG K Y,DING X M,et al.Effects of dietary supplementation with 25-OH-D3 on antibody and cytokine levels in serum and intestinal contents of weanling pigs with or without rotavirus challenge[J].Chinese Journal of Animal Nutrition,2011,23(1):34-42.(in Chinese)
[12] 张艳梅.羟基-D3的生理功能和机理研究[J].山西农业科学,2006,34(2):76-78. ZHANG Y M.Physiological function and mechanism of OH-D3[J].Journal of Shanxi Agricultural Sciences,2006,34(2):76-78.(in Chinese)
[13] AOAC.Official methods of analysis[M].Washington D.C.:Association of Official Analytical Chemists,1984:152-169.
[14] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.  
[15] FRITTS C A,WALDROUP P W.Effect of source and level of vitamin D on live performance and bone development in growing broilers[J].Journal of Applied Poultry Research,2003,12(1):45-52.  
[16] MAALOUF N M.The noncalciotropic actions of vitamin D:recent clinical developments[J].Current Opinion in Nephrology and Hypertension,2008,17(4):408-415.  
[17] MITCHELL R D,EDWARDS H M,Jr,MCDANIEL G R.The effects of ultraviolet light and cholecalciferol and its metabolites on the development of leg abnormalities in chickens genetically selected for a high and low incidence of tibial dyschondroplasia[J].Poultry Science,1997,76(2):346-354.  
[18] CELI P,WILLIAMS S,ENGSTROM M,et al.Safety evaluation of dietary levels of 25-hydroxyvitamin D3 in growing calves[J].Food and Chemical Toxicology,2018,111:641-649.
[19] NONNECKE B J,MCGILL J L,RIDPATH J F,et al.Acute phase response elicited by experimental bovine diarrhea virus (BVDV) infection is associated with decreased vitamin D and E status of vitamin-replete preruminant calves[J].Journal of Dairy Science,2014,97(9):5566-5579.  
[20] DUFFY S K,KELLY A K,RAJAURIA G,et al.The effect of 25-hydroxyvitamin D3 and phytase inclusion on pig performance,bone parameters and pork quality in finisher pigs[J].Journal of Animal Physiology and Animal Nutrition,2018,102(5):1296-1305.  
[21] ADHIKARI R,WHITE D,HOUSE J D,et al.Effects of additional dosage of vitamin D3,vitamin D2,and 25-hydroxyvitamin D3 on calcium and phosphorus utilization,egg quality and bone mineralization in laying hens[J].Poultry Science,2020,99(1):364-373.  
[22] BECHDEL S I,HILSTON N W,GUERRANT N B.The vitamin D requirement (U.S.P Units) for growth and well-being of calves from birth to six months of age[J].Journal of Dairy Science,1937,20(7):434-435.
[23] LITTLEDIKE E T,HORST R L.Vitamin D3 toxicity in dairy cows[J].Journal of Dairy Science,1982,65(5):749-759.  
[24] HORST R L,GOFF J P,REINHARDT T A.Calcium and vitamin D metabolism in the dairy cow[J].Journal of Dairy Science,1994,77(7):1936-1951.  
[25] XU H J,JIANG X,ZHANG C R,et al.Effects of dietary 25-hydroxyvitamin D3 on the lactation performance,blood metabolites,antioxidant and immune function in dairy cows[J].Livestock Science,2021,248:104497.
[26] VON ROSENBERG S J,WEBER G M,ERHARDT A,et al.Tolerance evaluation of overdosed dietary levels of 25-hydroxyvitamin D3 in growing piglets[J].Journal of Animal Physiology and Animal Nutrition,2016,100(2):371-380.  
[27] YANG J W,TIAN G,CHEN D W,et al.Effects of dietary 25-hydroxyvitamin D3 supplementation on growth performance,immune function and antioxidative capacity in weaned piglets[J].Archives of Animal Nutrition,2019,73(1):44-51.  
[28] FRANCESCHI R T,YOUNG J.Regulation of alkaline phosphatase by 1,25-dihydroxyvitamin D3 and ascorbic acid in bone-derived cells[J].Journal of Bone and Mineral Research,2009,5(11):1157-1167.  
[29] 黄伟,夏明月,李明丽,等.急性腹泻患儿血清25-羟维生素D与炎性细胞因子水平的关系[J].山东医药,2017,57(10):58-59. HUANG W,XIA M Y,LI M L,et al.Relationship between serum 25-hydroxyvitamin D and levels of inflammatory cytokines in children with acute diarrhea[J].Shandong Medical Journal,2017,57(10):58-59.(in Chinese)
[30] MVLLER K,ODUM N,BENDTZEN K.1,25-dihydroxyvitamin D3 selectively reduces interleukin-2 levels and proliferation of human T cell lines in vitro[J].Immunology Letters,1993,35(2):177-182.  
[31] KIM H S,WHON T W,SUNG H,et al.Longitudinal evaluation of fecal microbiota transplantation for ameliorating calf diarrhea and improving growth performance[J].Nature Communications,2021,12(1):161.
[32] 王新华,张星星,王立民,等.转IGF-1基因超细毛羊与非转基因羊肠道粪便菌群多样性及组成差异分析[J].畜牧兽医学报,2020,51(10):2387-2402. WANG X H,ZHANG X X,WANG L M,et al.Differences of the intestinal microbial flora diversity and composition in IGF-1 transgenic superfine wool sheep and non-transgenic sheep[J].Acta Veterinaria et Zootechnica Sinica,2020,51(10):2387-2402.(in Chinese)
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