研究论文 RESEARCH PAPER

甘蔗尾或甘蔗与构树叶混合青贮对其发酵品质的影响

  • 周晓康 ,
  • 张男吉 ,
  • 张洁 ,
  • 顾啟超 ,
  • 郑一民 ,
  • 麻艳群 ,
  • 邹彩霞
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  • 广西大学动物科学与技术学院, 南宁 530003
周晓康(1995-),男,四川遂宁人,硕士研究生,研究方向为反刍动物营养。E-mail:xiaokangzhou2019@gmail.com

收稿日期: 2021-05-13

  网络出版日期: 2022-01-18

基金资助

国家自然科学基金项目(31860661)

Effects of Mixed Silage of Sugarcane Top or Sugarcane and Broussonetia papyrifera Leaf on Its Fermentation Quality

  • ZHOU Xiaokang ,
  • ZHANG Nanji ,
  • ZHANG Jie ,
  • GU Qichao ,
  • ZHENG Yimin ,
  • MA Yanqun ,
  • ZOU Caixia
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  • College of Animal Science and Technology, Guangxi University, Nanning 530003, China

Received date: 2021-05-13

  Online published: 2022-01-18

摘要

本试验旨在通过混合青贮的方式,探究甘蔗尾或甘蔗与构树叶混合青贮对其发酵品质的影响,为改善广西地区的非常规饲料资源利用率和混合青贮饲料的实际应用提供科学依据和技术支撑。试验1:甘蔗尾与构树叶混合青贮;试验2:甘蔗与构树叶混合青贮。甘蔗尾或甘蔗与构树叶的混合比例分别为100:0、75:25、50:50、25:75、0:100。试验1结果表明:1)与甘蔗尾单独青贮相比,甘蔗尾与构树叶混合青贮显著增加了混合青贮饲料的粗蛋白质、乙酸含量(P<0.05),显著提高了有氧暴露后乙酸和丙酸的含量(P<0.05),并显著降低了肠杆菌数量(P<0.05)。2)与构树叶单独青贮相比,构树叶与甘蔗尾混合青贮显著增加了混合青贮饲料的可溶性碳水化合物含量(P<0.05);25%构树叶与75%甘蔗尾混合青贮显著降低了混合青贮饲料的pH和氨态氮含量(P<0.05)。试验2结果表明:1)与甘蔗单独青贮相比,甘蔗与构树叶混合青贮显著降低了混合青贮饲料的乙醇含量(P<0.05)。2)与构树叶单独青贮相比,25%构树叶与75%甘蔗混合青贮显著增加了混合青贮饲料的粗蛋白质、可溶性碳水化合物、乳酸和乙酸含量(P<0.05),同时显著降低了pH、氨态氮和丁酸含量(P<0.05),并使混合青贮饲料有氧暴露后的pH更稳定,同时大幅抑制霉菌的增长。综上可知,与构树叶混合青贮时,甘蔗占比75%能显著改善混合青贮饲料的发酵品质,而甘蔗尾的占比需要超过75%才能明显改善混合青贮饲料的发酵品质。

本文引用格式

周晓康 , 张男吉 , 张洁 , 顾啟超 , 郑一民 , 麻艳群 , 邹彩霞 . 甘蔗尾或甘蔗与构树叶混合青贮对其发酵品质的影响[J]. 动物营养学报, 2022 , 34(1) : 516 -532 . DOI: 10.3969/j.issn.1006-267x.2022.01.048

Abstract

This experiment was conducted to investigate the effects of mixed silage of sugarcane top or sugarcane and Broussonetia papyrifera leaf on fermentation quality through mixed silage, and provide scientific basis and technology for improving the utilization rate of unconventional feed resources in Guangxi and the practical application of mixed silage support. Experiment 1:mixed silage of sugarcane top and Broussonetia papyrifera leaf; experiment 2:mixed silage of sugarcane and Broussonetia papyrifera leaf. The mixing ratios of the two raw materials were 100:0, 75:25, 50:50, 25:75 and 0:100, respectively. The results of experiment 1 showed as follows:1) compared with sugarcane top silage alone, the sugarcane top mixed silage with Broussonetia papyrifera leaf increased the crude protein (CP) and acetic acid content of the mixed silage, and increased the growth rate of acetic acid and propionic acid content after aerobic exposure, and also reduced the number of enterobacteria (P<0.05). 2) Compared with Broussonetia papyrifera leaf silage alone, Broussonetia papyrifera leaf mixed silage with sugarcane top significantly increased the water-soluble carbohydrates (WSC) content of the mixed silage (P<0.05); when 25% Broussonetia papyrifera leaf mixed with 75% sugarcane top silage, it could significantly reduce the pH and ammoniacal nitrogen (NH3-N) content of mixed silage (P<0.05). The results of experiment 2 showed as follows:1) compared with sugarcane silage alone, sugarcane mixed silage with Broussonetia papyrifera leaf significantly reduced the ethanol content of the mixed silage (P<0.05). 2) Compared with Broussonetia papyrifera leaf silage alone, Broussonetia papyrifera leaf mixed silage with 75% sugarcane significantly increased the contents of CP, WSC, lactic acid and acetic acid of the mixed silage (P<0.05), while significantly reduced the pH, NH3-N and butyric acid contents (P<0.05), and made the pH of the mixed silage more stable after aerobic exposure, while greatly inhibiting the growth of molds. In summary, when mixed with Broussonetia papyrifera leaf, the proportion of sugarcane is 75% can significantly improve the fermentation quality of the mixed silage; while the proportion of sugarcane top exceed 75% can obviously improve the fermentation quality of the mixed silage.

参考文献

[1] 艾庆辉,苗又青,麦康森.单宁的抗营养作用与去除方法的研究进展[J].中国海洋大学学报(自然科学版),2011,41(1):33-40. AI Q H,MIAO Y Q,MAI K S.The anti-nutritional effects and the degradations of dietary tannins:a review[J].Periodical of Ocean University of China (Natural Science Edition),2011,41(1):33-40.(in Chinese)
[2] 陈奕业,邓铭,李斌,等.不同添加剂对构树叶青贮品质的影响[J].黑龙江畜牧兽医,2019(14):120-124. CHEN Y Y,DENG M,LI B,et al.Effects of different additives on the quality of Broussonetia papyrifera leaf silage[J].Heilongjiang Animal Science and Veterinary Medicine,2019(14):120-124.(in Chinese)
[3] 黄峰,李浩,王坤,等.附生微生物对青贮发酵品质和有氧稳定性的影响[J].动物营养学报,2020,32(12):5976-5984. HUANG F,LI H,WANG K,et al.Effects of epiphytic microorganisms on fermentation quality and aerobic stability of silage[J].Chinese Journal of Animal Nutrition,2020,32(12):5976-5984.(in Chinese)
[4] WU Q L,GUO W Q,ZHENG H S,et al.Enhancement of volatile fatty acid production by co-fermentation of food waste and excess sludge without pH control:the mechanism and microbial community analyses[J].Bioresource Technology,2016,216:653-660.
[5] 张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2003. ZHANG L Y.Feed analysis and feed quality testing technology[M].Beijing:China Agricultural University Press,2003.(in Chinese)
[6] VAN SOEST P J.Development of a comprehensive system of feed analyses and its application to forages[J].Journal of Animal Science,1967,26(1):119-128.  
[7] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.饲料中粗纤维的含量测定过滤法:GB/T 6434-2006[S].北京:中国标准出版社,2006. General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,Standardization Administration of the People's Republic of China.Feeding stuffs-determination of crude fiber content method with intermediate filtration:GB/T 6434-2006[S].Beijing:Standards Press of China,2006.(in Chinese)
[8] MURPHY R P.A method for the extraction of plant samples and the determination of total soluble carbohydrates[J].Journal of the Science of Food and Agriculture,1958,9(11):714-717.  
[9] 姜军,徐仁扣,赵安珍.用酸碱滴定法测定酸性红壤的pH缓冲容量[J].土壤通报,2006,37(6):1247-1248. JIANG J,XU R K,ZHAO A Z.Determination of pH buffer capacity of acid red soils by acid-base titration[J].Chinese Journal of Soil Science,2006,37(6):1247-1248.(in Chinese)
[10] 王鹏,白春生,刘林,等.低温条件下混合乳酸菌制剂对芦苇发酵品质的影响[J].草地学报,2011,19(1):127-131. WANG P,BAI C S,LIU L,et al.Effects of lactic acid bacteria inoculant on the fermentation quality of reed grass (Phragmites australis Cav.Trin.ex Sterd.) at low temperature[J].Acta Agrestia Sinica,2011,19(1):127-131.(in Chinese)
[11] BRODERICK G A,KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].Journal of Dairy Science,1980,63(1):64-75.  
[12] PRYCE J D.A modification of the Barker-Summerson method for the determination of lactic acid[J].Analyst,1969,94(125):1151-1152.
[13] ERWIN E S,MARCO G J,EMERY E M.Volatile fatty acid analyses of blood and rumen fluid by gas chromatography[J].Journal of Dairy Science,1961,44(9):1768-1771.  
[14] 吴大林,郭静,石鸿辉.构树作为新型蛋白饲料的研究进展[J].中国猪业,2017,12(5):56-59. WU D L,GUO J,SHI H H.Research progress of Broussonetia papyrifera as a new protein feed[J].China Swine Industry,2017,12(5):56-59.(in Chinese)
[15] BRUNING C L,YOKOYAMA M T.Characteristics of live and killed brewer's yeast slurries and intoxication by intraruminal administration to cattle[J].Journal of Animal Science,1988,66(2):585-591.  
[16] KUNG L,Jr,SHAVER R D,GRANT R J,et al.Silage review:interpretation of chemical,microbial,and organoleptic components of silages[J].Journal of Dairy Science,2018,101(5):4020-4033.  
[17] PEIXOTO P V,BRUST L A C,BRITO M F,et al.Ethanol poisoning in cattle by ingestion of waste beer yeast in Brazil[M]//RIET-CORREA F,PFISTER J,SCHILD A L,et al.Poisoning by plants,mycotoxins and related toxins.Boston:CABI,2011:494-498.
[18] BERGEN W G,BYREM T M,GRANT A L.Ensiling characteristics of whole-crop small grains harvested at milk and dough stages[J].Journal of Animal Science,1991,69(4):1766-1774.  
[19] KUNG L,SHAVER R.Interpretation and use of silage fermentation analysis reports[J].Focus on Forage,2001,3(13):1-5.
[20] REN F Y,HE R C,ZHOU X K,et al.Dynamic changes in fermentation profiles and bacterial community composition during sugarcane top silage fermentation:a preliminary study[J].Bioresource Technology,2019,285:121315.
[21] GUAN H,YAN Y H,LI X L,et al.Microbial communities and natural fermentation of corn silages prepared with farm bunker-silo in Southwest China[J].Bioresource Technology,2018,265:282-290.
[22] HENDERSON N.Silage additives[J].Animal Feed Science and Technology,1993,45(1):35-56.  
[23] OETZEL G R.Herd-level ketosis-diagnosis and risk factors[C]//American Association of Bovine Practitioners 40th Annual Conference:Preconference Seminar 7C:Dairy Herd Problem Investigation Strategies:Transition Cow Troubleshooting,Vancouver:AABP,2007.
[24] DENNY J,BHAT M,ECKMANN K.Outbreak of Escherichia coli O157:H7 associated with raw milk consumption in the Pacific Northwest[J].Foodborne Pathogens and Disease,2008,5(3):321-328.  
[25] GOTLIEB A.Mycotoxins in silage:a silent loss in profits[EB/OL].[2004-11-25] https://www.doc-developpement-durable.org/file/Culture/Culture-plantes-alimentaires/FICHES_PLANTES/ble-cereales/maladies/7.1c_mycotoxins_in_silage.pdf.
[26] KOROSTELEVA S N,SMITH T K,BOERMANS H J.Effects of feed naturally contaminated with Fusarium mycotoxins on metabolism and immunity of dairy cows[J].Journal of Dairy Science,2009,92(4):1585-1593.  
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