动物营养学报 >

2022 , Vol. 34 >Issue 1: 533 - 543

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.049

研究论文 RESEARCH PAPER

饲料中维生素K3含量对大口黑鲈消化能力、血清代谢指标、肌肉氨基酸和脂肪酸组成的影响

  • 魏翔 ,
  • 颜克涛 ,
  • 戴宇峰 ,
  • 华雪铭 ,
  • 李向 ,
  • 杭莹
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  • 1. 上海海洋大学, 农业农村部鱼类营养与环境生态研究中心, 上海 201306;
    2. 上海海洋大学, 农业农村部淡水水产种质资源重点实验室, 上海 201306;
    3. 上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306;
    4. 王屋水库管理所, 龙口 265721;
    5. 常州市天宁区郑陆畜牧兽医站, 常州 213111
魏翔(1995-),男,江苏连云港人,硕士研究生,从事水产动物营养学研究。E-mail:981820800@qq.com

收稿日期: 2021-05-06

  网络出版日期: 2022-01-18

基金资助

水产动物遗传育种协同创新基金(D-8006-13-0025)

收起

Effects of Dietary Vitamin K3 Content on Digestive Ability, Serum Metabolism Indexes, Muscle Amino Acid and Fatty Acid Composition of Largemouth Bass (Micropterus salmoides)

  • WEI Xiang ,
  • YAN Ketao ,
  • DAI Yufeng ,
  • HUA Xueming ,
  • LI Xiang ,
  • HANG Ying
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  • 1. Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;
    2. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;
    3. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;
    4. Administration Bureau of Wangwu Revervoir, Longkou 265721, China;
    5. Zhenglu Animal Husbandry and Veterinary Station, Changzhou 213111, China

Received date: 2021-05-06

  Online published: 2022-01-18

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摘要

为研究饲料中维生素K3含量对大口黑鲈消化能力、血清代谢指标、肌肉氨基酸和脂肪酸组成的影响，在基础饲料中分别添加不同水平的维生素K3，配制成维生素K3含量分别为0.78（K0组）、5.80（K5组）、10.82（K10组）、15.84（K15组）和20.85 mg/kg（K20组）的5种试验饲料，对初均重为（12.96±0.07）g健康大口黑鲈幼鱼进行为期8周的饲养试验。结果表明：添加维生素K3的各组胃和肠道的蛋白酶、淀粉酶和脂肪酶活性均显著低于K0组（P<0.05）。K10组、K15组和K20组的表观消化率和蛋白质表观消化率显著低于K0组（P<0.05）。K20组的血清尿素氮含量显著高于其他4组（P<0.05），血清总蛋白、白蛋白、总胆固醇和甘油三酯含量在各组之间无显著差异（P>0.05）。肌肉必需氨基酸、非必需氨基酸和总氨基酸含量以及必需氨基酸指数在各组之间无显著差异（P>0.05），但是随着饲料中维生素K3含量的增加，必需氨基酸和总氨基酸含量呈增加的趋势。K15组的肌肉单不饱和脂肪酸含量显著高于其他4组（P<0.05），其中C16：1n7和C18：1n5含量均较其他组显著升高（P<0.05）。肌肉饱和脂肪酸和多不饱和脂肪酸含量在各组之间无显著差异（P>0.05）。综上可知，饲料中添加维生素K3可以改善大口黑鲈肌肉氨基酸组成，但当饲料中维生素K3含量≥ 5.80 mg/kg时会使大口黑鲈的消化能力下降，并且高剂量的维生素K3会改变大口黑鲈的蛋白质和脂质代谢。

关键词： 大口黑鲈; 维生素K3; 消化; 代谢; 肌肉组成

本文引用格式

魏翔 , 颜克涛 , 戴宇峰 , 华雪铭 , 李向 , 杭莹 . 饲料中维生素K3含量对大口黑鲈消化能力、血清代谢指标、肌肉氨基酸和脂肪酸组成的影响[J]. 动物营养学报, 2022 , 34(1) : 533 -543 . DOI: 10.3969/j.issn.1006-267x.2022.01.049

Abstract

An 8-week feeding trail was carried out to investigate the effects of dietary vitamin K3 content on the digestive ability, serum metabolism indexes, muscle amino acid and fatty acid composition of largemouth bass (Micropterus salmoides). vitamin K3 was supplemented into a basal diet with the levels of 0.78 (K0 group), 5.80 (K5 group), 10.82 (K10 group), 15.84 (K15 group) and 20.85 mg/kg (K20 group), and then the five experimental diets were fed to healthy juvenile largemouth bass with the initial average body weight of (12.96±0.07) g. The results showed that the activities of protease, amylase and lipase in the stomach and intestine of the groups with vitamin K3 supplementation were significantly lower than those of K0 group (P<0.05). The apparent digestibility and protein apparent digestibility of K10, K15 and K20 groups were significantly lower than those of K0 group (P<0.05). The serum urea nitrogen content of K20 group was significantly higher than that in the other four groups (P<0.05), but there were no significant differences in the serum total protein, albumin, total cholesterol and triglyceride contents among the five groups (P>0.05). There were no significant differences in the contents of muscle essential amino acids, non-essential amino acids and total amino acids among all groups (P>0.05), essential amino acid index as well, but with the increase of dietary vitamin K3 content, the contents of essential amino acids and total amino acids showed an increasing trend. The content of muscle monounsaturated fatty acids of K15 group was significantly higher than that of other four groups (P<0.05), and the contents of C16:1n7 and C18:1n5 were significantly increased compared with other groups (P<0.05). There were no significant differences in the contents of muscle saturated fatty acids and polyunsaturated fatty acids among all groups (P>0.05). In summary, dietary supplementation of vitamin K3 can improve the muscle amino acid composition, but it decreases the digestive ability when the vitamin K3 content is equal or greater than 5.80 mg/kg, and high dose of dietary vitamin K3 changes protein and fatty acid metabolism.

Key words： largemouth bass; vitamin K3; digestion; metabolism; muscle composition

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