研究论文 RESEARCH PAPER

饲粮中联合添加牛至精油和抗菌肽对肉兔生长性能和肠道屏障的影响

  • 牛金玲 ,
  • 刘永需 ,
  • 李福昌 ,
  • 刘磊
展开
  • 1. 山东农业大学动物科技学院, 山东省动物生物工程与疾病防治重点实验室, 泰安 271000;
    2. 青岛康大食品有限公司, 青岛 266555
牛金玲(1996-),女,山东淄博人,硕士研究生,从事家兔饲料资源的开发利用。E-mail:jinniu0312@163.com

收稿日期: 2021-06-27

  网络出版日期: 2022-01-18

基金资助

现代农业产业技术体系建设专项(CARS-43-B-1);泰山产业领军项目(TSCY20190107);山东省自然科学基因(ZR202102200454);山东省博士后创新项目(2020年);新疆自治区重点研发计划项目(2020B01004-1-3)

Effects of Combined Dietary Oregano Essential Oil and Antimicrobial Peptides on Growth Performance and Intestinal Barrier in Meat Rabbits

  • NIU Jinling ,
  • LIU Yongxu ,
  • LI Fuchang ,
  • LIU Lei
Expand
  • 1. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Anima lScience and Technology, Shandong Agricultural University, Tai'an 271000, China;
    2. Qingdao Kangda Food Co., Ltd., Qingdao 266555, China

Received date: 2021-06-27

  Online published: 2022-01-18

摘要

本试验旨在研究饲粮中联合添加牛至精油和抗菌肽对生长肉兔生长性能和肠道屏障的影响。试验选用324只初始体重相近的38日龄健康断奶肉兔,随机分为2组,每组9个重复,每个重复18只兔。对照组饲喂基础饲粮,试验组在基础饲粮中联合添加0.08%的牛至精油和0.15%的抗菌肽。试验期为30 d。结果表明:与对照组相比,试验组肉兔的平均日增重显著增加(P<0.05),料重比和腹泻率显著降低(P<0.05);试验组空肠和回肠的绒毛高度及回肠的绒毛高度/隐窝深度显著增加(P<0.05);饲粮联合添加牛至精油和抗菌肽显著增加空肠中闭合蛋白1(Claudin1)和连接黏附分子3基因相对表达量(P<0.05),同时也显著降低了回肠中Claudin1基因相对表达量(P<0.05);饲粮联合添加牛至精油和抗菌肽显著升高了空肠中白细胞介素-10和回肠中分泌型免疫球蛋白G的含量(P<0.05),却显著降低了空肠中肿瘤坏死因子-α的含量(P<0.05)。综上所述,在饲粮中联合添加0.08%牛至精油及0.15%抗菌肽提高了肉兔的平均日增重,降低了肉兔的料重比和腹泻率,提高了肠道的机械屏障和免疫屏障,提高了机体免疫力。

本文引用格式

牛金玲 , 刘永需 , 李福昌 , 刘磊 . 饲粮中联合添加牛至精油和抗菌肽对肉兔生长性能和肠道屏障的影响[J]. 动物营养学报, 2022 , 34(1) : 555 -562 . DOI: 10.3969/j.issn.1006-267x.2022.01.051

Abstract

The aim of this experiment was to investigate the effects of combined dietary oregano essential oil and antimicrobial peptides on growth performance and intestinal barrier in meat rabbits. Three hundred and twenty-four healthy weaned meat rabbits (35-day-old) with similar initial body weight were randomly divided into two groups (nine replicates per group, 18 rabbits in each replicate):control group fed a basal diet and experiment group fed the basal diet with an addition of 0.08% oregano essential oil and 0.15% antimicrobial peptide. The test period was 30 days. The results showed that combined dietary oregano essential oil and antimicrobial peptide significantly increased the average daily body weight (P<0.05), but decreased the rabbit feed/gain and diarrhea rate (P<0.05) compared with the control group. The villus height of jejunum and ileum, as well as the ratio of villus height to crypt depth in ileum were significantly increased after oregano essential oil and antimicrobial peptide treatment (P<0.05). Combined dietary oregano essential oil and antimicrobial peptide significantly increased the relative expression levels of Claudin1 and connect the adhesion molecule 3 (JAM3) genes in jejunum (P<0.05), and significantly decreased the relative expression level of Claudin1 gene in ileum (P<0.05). Combined dietary oregano essential oil and antimicrobial peptide treatment significantly increased the contents of interleukin-10 and secreted immunoglobulin G in jejunum (P<0.05), but significantly decreased the content of tumor necrosis factor-α in jejunum (P<0.05). In conclusion, combined dietary 0.08% oregano essential oil and 0.15% antimicrobial peptide can increase the average daily body weight, reduce the diarrhea rate of meat rabbits, improve the intestinal mechanical barrier and immune barrier, and improve the body immunity.

参考文献

[1] 时志琪,连凯琪,林正丹,等.抗菌肽杀菌效果、机制研究方法进展[J].中国畜禽种业,2020,16(11):52-53. SHI Z Q,LIAN K Q,LIN Z D,et al.Advances in research methods on bactericidal effects and mechanisms of antimicrobial peptides[J].The Chinese Livestock and Poultry Breeding,2020,16(11):52-53.(in Chinese)
[2] 何兰花.新型植物抗生素-牛至油[J].畜牧与兽医,2004,36(5):22-23. HE L H.New plant-based antibiotic-oregano oil[J].Animal Husbandry & Veterinary Medicine,2004,36(5):22-23.(in Chinese)
[3] BOTSOGLOU N A,FLOROU-PANERI P,CHRISTAKI E,et al.Performance of rabbits and oxidative stability of muscle tissues as affected by dietary supplementation with oregano essential oil[J].Archives of Animal Nutrition,2004,58(3):209-218.  
[4] 牛金玲.牛至精油对生长肉兔生产性能及肠道屏障的影响[D].硕士学位论文.泰安:山东农业大学,2021. NIU J L.Effect of oregano essential oil on the productive performance and intestinal barrier of growing meat rabbits[D].Master's Thesis.Tai'an:Shandong Agricultural University,2021.(in Chinese)
[5] 王曦尧.抗菌肽对吉戎兔生长性能和繁殖性能的影响[D].硕士学位论文.长春:吉林大学,2016. WANG X Y.Effects of antibacterial peptides on growth performance and reproductive performance of Jirong rabbits[D].Master's Thesis.Changchun:Jilin University,2016.(in Chinese)
[6] 王红娜,杨振燕,杨志伟,等.抗菌肽制剂对肉鸡生产性能的影响[C]//中国畜牧兽医学会,中国畜牧兽医学会2018年学术年会禽病学分会第十九次学术研讨会论文集,南宁:中国畜牧兽医学会,2018:1. WANG H N,YANG Z Y,YANG Z W,et al.Effect of antimicrobial peptide preparations on the performance of broiler chickens[C]//Chinese Society of Animal Husbandry and Veterinary Medicine,Proceedings of the 19th Symposium of the Avian Branch of the 2018 Academic Annual Meeting of the Chinese Society of Animal Husbandry and Veterinary Medicine,Nanning:Chinese Society of Animal Husbandry and Veterinary Medicine,2018:1.(in Chinese)
[7] 徐博成,李智,汪以真,等.抗菌肽对仔猪生长性能、腹泻率和免疫球蛋白水平影响的Meta分析[J].动物营养学报,2020,32(8):3584-3593. XU B C,LI Z,WANG Y Z,et al.Effects of antimicrobial peptides on growth performance,diarrhea rate and immunoglobulin levels of piglets:a Meta-analysis[J].Chinese Journal of Animal Nutrition,2020,32(8):3584-3593.(in Chinese)
[8] 杨福剑,张宗尧,何胜学,等.枯草芽孢杆菌与植物精油组合对广西地方肉种鸡生产性能的影响[J].饲料博览,2020(11):6-9. YANG F J, ZHANG Z Y, HE S X, et al.Effects of combination of Bacillus subtilis and essential oils on performance of local broiler breeders in Guangxi[J].Feed Review,2020(11):6-9.(in Chinese)
[9] 苗旭,刘彦,贺军,等.抗菌肽对肉鸡屠宰性能和肉品质影响的研究[J].国外畜牧学(猪与禽),2020,40(10):27-31. MIAO X,LIU Y,HE J,et al.Study on the effect of antimicrobial peptides on slaughter performance and meat quality of broiler chickens[J].Animal Science Abroad(Pigs and Poultry),2020,40(10):27-31.(in Chinese)
[10] 董国权,郭琛琛,王宝晴.牛至油对育肥猪生长性能和肉品质的影响[J].中国饲料,2019(12):34-38. DONG G Q,GUO C C,WANG B Q.Effects of dietary supplementation of oregano oil on growth performance and meat quality of growing pigs[J].China Feed,2019(12):34-38.(in Chinese)
[11] 张金贵.牛至油对蛋鸡生产性能和腹泻率病死率的影响[D].硕士学位论文.通辽:内蒙古民族大学.2013. ZHANG J G.Effects of oregano oil on performance and diarrhea fatality rate in laying hens[D].Master's Thesis.Tongliao:Inner Mongolia Minzu University,2016.(in Chinese)
[12] 宋延飞.抗菌肽替代抗生素在断奶仔猪中的应用研究[J].饲料工业.2018,39(4):17-20. SONG Y F.Application of antimicrobial peptides instead of antibiotics in weaned piglets[J].Feed Industry,2018,39(4):17-20.(in Chinese)
[13] 刘静慧,李冲,徐美利,等.绿原酸对肉兔生长性能、空肠结构与抗氧化的影响[J].中国畜牧杂志,2021,57(6):205-210. LIU J H,LI C,XU M L,et al.Effects of chlorogenic acid on growth performance,jejunum structure and jejunum antioxidant activity of meat rabbits[J].Chinese Journal of Animal Science,2021,57(6):205-210.(in Chinese)
[14] MOHITI-ASLI M,GHANAATPARAST-RASHTI M.Dietary oregano essential oil alleviates experimentally induced coccidiosis in broilers[J].Preventive Veterinary Medicine,2015,120(2):195-202.  
[15] TEKCE E,ÇINAR K,BAYRAKTAR B,et al.Effects of an essential oil mixture added to drinking water for temperature-stressed broilers:performance,meat quality,and thiobarbituric acid-reactive substances[J].Journal of Applied Poultry Research,2020,29(1):77-84.  
[16] 黄熠.玉屏风散和抗菌肽对肉鸡生产性能的影响[D].硕士学位论文.广州:华南农业大学,2016. HUANG Y.Effects of Jade screen powder and antibacterial peptide on the performance of broilers[D].Master's Thesis.Guangzhou:South China Agricultural University,2016.(in Chinese)
[17] YOSEPH B P,KLINGENSMITH N J,LIANG Z,et al.Mechanisms of intestinal barrier dysfunction in sepsis[J].Shock,2016,46(1):52-59.  
[18] 赵晓宇.大蒜秸对生长肉兔饲用效果的评价[D].硕士学位论文.泰安:山东农业大学,2018. ZHAO X Y.The evaluation of the feeding effects of garlic straw in growing meat rabbit[D].Master's Thesis.Tai'an:Shandong Agricultural University,2018.(in Chinese)
[19] RAJASEKARAN A,SHIVAKUMAR V,KALMADY S V,et al.The impact of IL-10 polymorphisms and sHLA-G levels on the risk of schizophrenia[J].Asian Journal of Psychiatry,2016,23:39-43.
[20] KARSEN H,TEKIN KORUK S,DUYGU F,et al.Review of 17 cases of neurobrucellosis:clinical manifestations,diagnosis,and management[J].Archives of Iranian Medicine,2012,15(8):491-494.
[21] 郭明.抗菌肽和合生素对肉仔鸡生长性能和免疫功能的影响[D].硕士学位论文.郑州:河南农业大学,2016. GUO M.Antibacterial peptides and synbiotic effect on growth performance and immune function of broiler chickens[D].Master's Thesis.Zhengzhou:Henan Agricultural University,2016.(in Chinese)
文章导航

/