动物营养学报 >

2022 , Vol. 34 >Issue 1: 612 - 625

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.056

研究论文 RESEARCH PAPER

体外法研究竹叶黄酮对奶牛乳腺上皮细胞抗氧化及哺乳动物雷帕霉素靶蛋白信号通路中关键基因表达的影响

  • 王宇彤 ,
  • 沈义媛 ,
  • 霍相羽 ,
  • 牛慧 ,
  • 韩思雨 ,
  • 童津津 ,
  • 熊本海 ,
  • 蒋林树
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  • 1. 北京农学院动物科学技术学院, 奶牛营养学北京市重点实验室, 北京 102206;
    2. 中国农业科学院北京畜牧兽医研究所, 北京 100193
王宇彤(2000-),女,北京人,本科生,研究方向为奶牛营养与免疫。E-mail:wangyutong428@126.com

收稿日期: 2021-06-23

  网络出版日期: 2022-01-18

基金资助

北京市教委重点项目(20JF0008)

收起

In Vitro Study on Effects of Bamboo Leaf Flavonoids on Antioxidation and Expression of Key Genes in Mammalian Target of Rapamycin Signaling Pathway in Bovine Mammary Epithelial Cells

  • WANG Yutong ,
  • SHEN Yiyuan ,
  • HUO Xiangyu ,
  • NIU Hui ,
  • HAN Siyu ,
  • TONG Jinjin ,
  • XIONG Benhai ,
  • JIANG Linshu
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  • 1. College of Animal Science and Technology, Beijing University of Agriculture, Beijing Key Laboratory of Cow Nutrition, Beijing 102206, China;
    2. Beijing Institute of Animal Husbandry and Veterinary Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2021-06-23

  Online published: 2022-01-18

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摘要

本试验旨在通过体外法研究竹叶黄酮（BLF）对奶牛乳腺上皮细胞（BMECs）抗氧化及哺乳动物雷帕霉素靶蛋白（mTOR）信号通路中关键基因表达的影响。采用单因素试验设计，对照组不添加BLF，试验组分别添加不同浓度（0.5、1.0、5.0、10.0、20.0、60.0、100.0 μg/mL）BLF，处理BMECs 3、6、12、24和48 h后，应用细胞增殖试剂盒（CCK-8）法检测BMECs的活性，应用乳酸脱氢酶（LDH）法检测BLF对BMECs的毒性，每次试验平行复孔6个，重复试验3次。分别应用流式细胞仪和试剂盒法检测BLF对BMECs抗氧化指标的影响，并应用实时荧光定量PCR法检测凋亡相关基因以及mTOR信号通路中相关基因表达量的变化。结果表明：1）BLF对BMECs活性适宜作用浓度为1.0、5.0、10.0 μg/mL，最佳作用时间为12 h，且此浓度的BLF对BMECs无毒性作用。2）流式细胞仪检测结果表明，与对照组相比，5.0、10.0 μg/mL的BLF极显著降低了BMECs的早期凋亡率和晩期凋亡/坏死率（P<0.01），BLF极显著降低了BMECs的总凋亡率（P<0.01）。3）与对照组相比，5.0 μg/mL的BLF极显著降低了细胞内活性氧（ROS）的生成和线粒体膜电位（MMP）水平（P<0.01）。4）与对照组相比，添加BLF可极显著升高BMECs的总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）（P<0.01）活性极显著降低丙二醛（MDA）的含量（P<0.01）。5）实时荧光定量PCR的结果表明，与对照组相比，添加5.0 μg/mL的BLF显著降低B细胞淋巴瘤/白血病-2相关X蛋白（Bax）的基因相对表达量（P<0.05）；BLF可极显著提高B淋巴细胞瘤/白血病-2（Bcl-2）的基因相对表达量（P<0.01），极显著降低Bax/Bcl-2的比值（P<0.01）；添加5.0和10.0 μg/mL的BLF显著降低环氧合酶-2（COX-2）基因相对表达量（P<0.05），极显著降低半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）基因相对表达量（P<0.01），极显著提高真核翻译起始因子4E结合蛋白1（EIF-4EBP1）基因相对表达量（P<0.01）；此外，与对照组相比，BLF可极显著提高BMECs中mTOR信号通路关键调控基因mTOR、核糖体p70s6（S6K1）和细胞周期素D1（Cyclin D1）基因相对表达量（P<0.01），极显著提高β-酪蛋白（β-casein）基因相对表达量（P<0.01）。综上所述，BLF可促进BMECs的活性，减少ROS的积累和MMP水平；BLF可显著提高BMECs的抗氧化能力，降低凋亡相关基因的表达，通过mTOR信号通路转导，从而影响乳蛋白的合成。

关键词： 竹叶黄酮; 抗氧化; 细胞凋亡; mTOR信号通路

本文引用格式

王宇彤 , 沈义媛 , 霍相羽 , 牛慧 , 韩思雨 , 童津津 , 熊本海 , 蒋林树 . 体外法研究竹叶黄酮对奶牛乳腺上皮细胞抗氧化及哺乳动物雷帕霉素靶蛋白信号通路中关键基因表达的影响[J]. 动物营养学报, 2022 , 34(1) : 612 -625 . DOI: 10.3969/j.issn.1006-267x.2022.01.056

Abstract

The study was conducted to investigate the effects of bamboo leaf flavonoids(BLF)on antioxidant activity and expression of key genes in mammalian target of rapamycin (mTOR) signaling pathway in bovine mammary epithelial cells(BMECs) in vitro. Using a single factor experimental design, BMECs were treated with different concentrations of BLF(0, 0.5, 1.0, 5.0, 10.0, 20.0, 60.0, 100.0 μg/mL)for 3, 6, 12, 24 and 48 h without BLF as control group. The activity of BMECs was detected by CCK-8 assay, and the toxicity of BMECs was detected by lactic dehydrogenase (LDH) method. There were 6 parallel multiple holes in each test, and the test was repeated for 3 times. The effects of BLF on the antioxidant indices of BMECs was detected by flow cytometry and kit method. The changes of mRNA expression levels of apoptotic genes, and the key genes in mTOR signaling pathway were detected by fluorescence quantitative PCR. The results showed as follows:1) the optimum concentrations of BLF on BMECs activity were 1.0, 5.0, 10.0 μg/mL, and the best action time was 12 h. The concentrations of BLF had no toxic effect on BMECs. 2) The results of flow cytometry showed that 5.0 and 10.0 μg/mL BLF extremely significantly decreased the early apoptosis rate and apoptosis/necrosis rate of BMECs(P<0.01), and BLF extremely significantly decreased the total apoptosis rate of BMECs(P<0.01). 3) 5.0 μg/mL BLF extremely significantly decreased the production of intracellular reactive oxygen species (ROS) and the level of mitochondrial membrane potential (MMP)(P<0.01). 4) BLF supplementation extremely significantly increased the activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) (P<0.01), and extremely significantly decreased the content of MDA(P<0.01). 5) The results of fluorescence quantitative PCR showed that 5.0 μg/mL BLF significantly decreased the gene relative expression level of B-cell lymphoma/leukaemia-2-associated X protein (Bax)(P<0.05), BLF extremely significantly increased the gene relative expression level of B-cell lymphoma/leukaemia-2 (Bcl-2)(P<0.01), and extremely significantly decreased the ratio of Bax/Bcl-2 (P<0.01), 5.0 and 10.0 μg/mL BLF significantly decreased the gene relative expression level of cyclooxygenase-2 (COX-2) (P<0.05), and extremely significantly decreased the gene relative expression level of Caspase-3 (P<0.01), and extremely significantly increased the gene relative expression level of eukaryotic translation initiation factor 4E binding protein 1 (EIF-4EBP1)(P<0.01). In addition, BLF extremely significantly increased the gene relative expression level of mTOR, ribosome p70s6 (S6K1) and Cyclin D1 in BMECs (P<0.01), the key regulatory genes of mTOR signaling pathway in BMECs. BLF extremely significantly increased the gene expression levels of β-casein in BMECs(P<0.01). In conclusion, BLF can promote the activity of BMECs, reduce the accumulation of ROS and the level of MMP. BLF can significantly improve the antioxidant capacity of BMECs, reduce the expression of apoptosis-related genes, and affect the synthesis of milk protein by promoting mTOR signaling pathway.

Key words： bamboo leaf flavonoids; anti-oxidative; apoptosis; mTOR signaling pathway

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