研究论文 RESEARCH PAPER

发酵麸皮多糖对杜寒杂交肉羊瘤胃菌群及其屏障功能相关指标的影响

  • 王文文 ,
  • 王园 ,
  • 孟子琪 ,
  • 安晓萍 ,
  • 齐景伟
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  • 1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
    2. 内蒙古自治区草食家畜饲料工程技术研究中心, 呼和浩特 010018;
    3. 内蒙古自治区农牧业科学院动物营养与饲料研究所, 呼和浩特 010031
王文文(1993-),女,内蒙古呼和浩特人,博士研究生,从事生物饲料的研发与应用研究。E-mail:wangwenwen2017@emails.imau.edu.cn

收稿日期: 2021-06-10

  网络出版日期: 2022-01-18

基金资助

内蒙古自治区科技重大专项(2020ZD0004);内蒙古自然科学基金项目(2020MS03041);内蒙古自治区关键技术攻关计划项目(2020GG0030)

Effects of Fermented Wheat Bran Polysaccharides on Microbiota and Rumen Barrier Junction Related Indices of Dorper×Thin-Tailed Han Crossbred Meat Lambs

  • WANG Wenwen ,
  • WANG Yuan ,
  • MENG Ziqi ,
  • AN Xiaoping ,
  • QI Jingwei
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  • 1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Inner Mongolia Herbivorous Livestock Feed Engineering and Technology Research Center, Hohhot 010018, China;
    3. Institute of Animal Nutrition and Feed in Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China

Received date: 2021-06-10

  Online published: 2022-01-18

摘要

本试验旨在研究发酵麸皮多糖(FWBPs)对杜寒杂交肉羊瘤胃组织紧密连接蛋白和细胞因子基因表达及瘤胃菌群结构的影响。选取21只健康且体重[(21.85±0.64)kg]相近的6周龄杜寒杂交肉羊21只,随机分为3个组,每组7只羊。对照组、低剂量组和高剂量组分别在基础饲粮中添加0、200、400 mg/kg BW的FWBPs。预试期14 d,正试期56 d。结果表明:1)与对照组相比,低剂量组肉羊瘤胃组织中闭锁蛋白(occludin)、闭合蛋白-1(claudin-1)和闭合蛋白-4(claudin-4)的mRNA相对表达量显著增加(P<0.05),高剂量组肉羊瘤胃组织中occludin、claudin-1、claudin-4、闭合小环蛋白-1(ZO-1)、干扰素-γ(IFN-γ)和白细胞介素-6(IL-6)的mRNA相对表达量显著增加(P<0.05)。2)高剂量组肉羊瘤胃菌群Shannon指数和Simpson指数显著高于对照组和低剂量组(P<0.05)。与对照组相比,低剂量组肉羊瘤胃菌群中螺旋菌门(Spirochaetota)和琥珀酸弧菌属(Succinivibrio)的相对丰度显著增加(P<0.05);高剂量组肉羊瘤胃菌群中变形菌门(Proteobacteria)、琥珀酸弧菌科_UCG-001(Succinivibrionaceae_UCG-001)和解琥珀酸菌属(Succiniclasticum)的相对丰度显著或极显著降低(P<0.05或P<0.01),拟杆菌门(Bacteroidota)和新月形单胞菌属(Selenomonas)的相对丰度显著增加(P<0.05)。高剂量组肉羊瘤胃菌群中Proteobacteria、Spirochaetota和Succinivibrionaceae_UCG-001的相对丰度显著或极显著低于低剂量组(P<0.05或P<0.01),而Bacteroidota、韦荣球菌科_UCG-001(Veillonellaceae_UCG-001)和Selenomonas的相对丰度极显著高于低剂量组(P<0.01)。3)线性判别分析效应大小(LEfSe)分析显示,柔膜菌目(Erysipelotrichales)、氨基酸球菌科(Acidaminococcaceae)、氨基酸球菌目(Acidaminococcales)、Succiniclasticum在对照组中富集,琥珀酸弧菌科(Succinivibrionaceae)、气单胞菌目(Aeromonadales)、γ-变形菌纲(Gammaproteobacteria)、Proteobacteria、Succinivibrionaceae_UCG-001在低剂量组中富集,Bacteroidota、拟杆菌纲(Bacteroidia)、拟杆菌目(Bacteroidales)、普雷沃氏菌科(Prevotellaceae)、新月形单胞菌科(Selenomonadaceae)、栖瘤胃普雷沃氏菌(Prevotella_ruminicola)、Selenomonas、反刍兽月形单胞菌(Selenomonas_ruminantium)、Veillonellaceae_UCG-001在高剂量组中富集。综上所述,FWBPs能够提高杜寒杂交肉羊瘤胃组织中紧密连接蛋白和细胞因子的mRNA相对表达量,调控瘤胃菌群结构,维持瘤胃健康。

本文引用格式

王文文 , 王园 , 孟子琪 , 安晓萍 , 齐景伟 . 发酵麸皮多糖对杜寒杂交肉羊瘤胃菌群及其屏障功能相关指标的影响[J]. 动物营养学报, 2022 , 34(1) : 640 -650 . DOI: 10.3969/j.issn.1006-267x.2022.01.058

Abstract

This study was conducted to investigate the effects of fermented wheat bran polysaccharides (FWBPs) on expression of tight junction protein and cytokine genes in rumen tissue and rumen microbiota structure of Dorper×thin-tailed Han crossbred meat lambs. Twenty-one 6-week-old Dorper×thin-tailed Han crossbred meat lambs with similar body weight[(21.85±0.64) kg] were randomly assigned into three groups of seven lambs in each group. Lambs in the three groups were fed basal diets supplemented with 0 (control group), 200 (low dose group) and 400 mg/kg BW (high dose group) FWBPs, respectively. The pre-experimental period lasted for 14 days, and the experimental period lasted for 56 days. The results showed as follows:1) compared with the control group, the mRNA relative expression levels of occludin, claudin-1 and claudin-4 in rumen tissue of meat lambs of low dose group were significantly increased (P<0.05), and the mRNA relative expression levels of occludin, claudin-1, claudin-4, zonula occludens-1 (ZO-1) interferon-γ (IFN-γ) and interleukin-6 (IL-6) in rumen tissue of meat lambs of high dose group were significantly increased (P<0.05). 2) The Shannon index and Simpson index in rumen microbiota of meat lambs of high dose group were significantly higher than those of the control and low dose group (P<0.01). Compared with the control group, the relative abundances of Spirochaetota and Succinivibrio in rumen microbiota of meat lambs of low dose group were significantly increased (P<0.05); the relative abundances of Proteobacteria, Succinivibrionaceae_UCG-001 and Succiniclasticum in rumen microbiota of meat lambs of high dose group were significantly decreased (P<0.05 or P<0.01), and the Bacteroidota and Selenomonas relative abundances were significantly increased (P<0.05). The relative abundances of Proteobacteria, Spirochaetota and Succinivibrionaceae_UCG-001 in rumen microbiota of meat lambs of high dose group were significantly lower than those of low dose group (P<0.05 or P<0.01), while the Bacteroidota, Veillonellaceae_UCG-001 and Selenomonas relative abundances were significantly higher than those of low dose group (P<0.01). 3) The linear discriminant analysis effect size (LEfSe) analysis showed that Erysipelotrichales, Acidaminococcaceae, Acidaminococcales and Succiniclasticum were enriched in the control group, the Succinivibrionaceae, Aeromonadales, Gammaproteobacteria, Proteobacteria and Succinivibrionaceae_UCG-001 were enriched in low dose group, and the Bacteroidota, Bacteroidia, Bacteroidales, Prevotellaceae, Selenomonadaceae, Prevotella_ruminicola, Selenomonas, Selenomonas_ruminantium and Veillonellaceae_UCG-001 were enriched in high dose group. In conclusion, FWBPs can increase tight junction protein and cytokine mRNA relative expression levels of Dorper×thin-tailed Han crossbred meat lambs, regulate the rumen microbiota structure, and maintain the rumen health.

参考文献

[1] ASCHENBACH J R,PENNER G B,STUMPFF F,et al.Ruminant nutrition symposium:role of fermentation acid absorption in the regulation of ruminal pH[J].Journal of Animal Science,2011,89(4):1092-1107.  
[2] YÁÑEZ-RUIZ D R,ABECIA L,NEWBOLD C J.Manipulating rumen microbiome and fermentation through interventions during early life:a review[J].Frontiers in Microbiology,2015,6:1133.
[3] JING X P,WANG W J,DEGEN A,et al.Tibetan sheep have a high capacity to absorb and to regulate metabolism of SCFA in the rumen epithelium to adapt to low energy intake[J].The British Journal of Nutrition,2020,123(7):721-736.  
[4] SUN Y Y,CHENG M,XU M,et al.The effects of subacute ruminal acidosis on rumen epithelium barrier function in dairy goats[J].Small Ruminant Research,2018,169:1-7.
[5] STEELE M A,CROOM J,KAHLER M,et al.Bovine rumen epithelium undergoes rapid structural adaptations during grain-induced subacute ruminal acidosis[J].American Journal of Physiology.Regulatory,Integrative and Comparative Physiology,2011,300(6):R1515-R1523.
[6] 李颖.猴头菇多糖缓解山羊亚急性瘤胃酸中毒效果及作用机理[D].硕士学位论文.福州:福建农林大学,2019. LI Y.The effect and mechanism of Hericium erinaceus polysaccharides mitigating SARA in goat[D].Master's Thesis.Fuzhou:Fujian Agriculture and Forestry University,2019.(in Chinese)
[7] CHEN Q Y,WANG R F,WANG Y,et al.Characterization and antioxidant activity of wheat bran polysaccharides modified by Saccharomyces cerevisiae and Bacillus subtilis fermentation[J].Journal of Cereal Science,2021,97:103157.
[8] 王园,史俊祥,段元霄,等.麸皮多糖微生物发酵工艺优化及其抗炎活性[J].食品科学,2018,39(14):192-198. WANG Y,SHI J X,DUAN Y X,et al.Optimization of fermentation conditions for improved production of polysaccharides from wheat bran and anti-inflammatory effects of the extracted polysaccharides[J].Food Science,2018,39(14):192-198.(in Chinese)
[9] 王文文,王园,郝希然,等.发酵麸皮多糖对大鼠组织细胞因子含量及盲肠菌群结构的影响[J].动物营养学报,2019,31(6):2865-2874. WANG W W,WANG Y,HAO X R,et al.Effects of fermented wheat bran polysaccharides on tissue cytokine contents and caecal microflora structure of rats[J].Chinese Journal of Animal Nutrition,2019,31(6):2865-2874.(in Chinese)
[10] 王园,杨可心,段元霄,等.发酵麸皮多糖对大鼠空肠组织抗氧化能力、形态结构和紧密连接蛋白表达的影响[J].食品科学,2019,40(13):166-170. WANG Y,YANG K X,DUAN Y X,et al.Effects of fermented wheat bran polysaccharides on antioxidant capacity,morphological structure and tight junction protein expression in rat jejunum[J].Food Science,2019,40(13):166-170.(in Chinese)
[11] 孟子琪.发酵麸皮多糖对杜寒杂交肉羊生长性能、肉品质及血液抗氧化指标的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2018. MENG Z Q.Effect of dietary fermented wheat bran polysaccharides supplementation on growth performance,meat quality and plasma antioxidant indexes of Dorper×thin-tailed Han crossbred meat lambs[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University,2018.(in Chinese)
[12] 史俊祥.麸皮多糖微生物发酵制备及其粗制品抗氧化活性的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2017. SHI J X.Research on the microbial fermentation of wheat bran polysaccharide and the antioxidant activity of crude polysaccharide[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University,2017.(in Chinese)
[13] OSHIMA T,KOSEKI J,CHEN X,et al.Acid modulates the squamous epithelial barrier function by modulating the localization of claudins in the superficial layers[J].Laboratory Investigation,2012,92(1):22-31.  
[14] PORITZ L S,HARRIS L R 3rd,KELLY A A,et al.Increase in the tight junction protein claudin-1 in intestinal inflammation[J].Digestive Diseases and Sciences,2011,56(10):2802-2809.  
[15] 李碧波.绒山羊胃肠道微生物区系及其对日粮响应的研究[D].博士学位论文.杨凌:西北农林科技大学,2020. LI B B.Study on gastro-intestinal microbial flora and its response to diet in cashmere goat[D].Ph.D.Thesis.Yangling:Northwest A & F University,2020.(in Chinese)
[16] 杜芳瑜,薛盖君,刘中博,等.细胞因子风暴及其治疗方法的研究进展[J].中国药物化学杂志,2021,31(1):39-54. DU F Y,XUE G J,LIU Z B,et al.Research advances of cytokine storm and therapeutics[J].Chinese Journal of Medicinal Chemistry,2021,31(1):39-54.(in Chinese)
[17] NAZARENKO E L,KOMANDROVA N A,GORSHKOVA R P,et al.Structures of polysaccharides and oligosaccharides of some Gram-negative marine Proteobacteria[J].Carbohydrate Research,2003,338(23):2449-2457.  
[18] CHEN X D,SU X D,LI J L,et al.Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis[J].NPJ Biofilms and Microbiomes,2021,7(1):45.
[19] 金鹿,李胜利,桑丹,等.沙蒿多糖组合制剂对滩羊羔羊瘤胃菌群多样性的影响[J].动物营养学报,2021,33(1):317-329. JIN L,LI S L,SANG D,et al.Effects of Artemisia polysaccharide combination preparation on rumen microflora diversity of Tan lambs[J].Chinese Journal of Animal Nutrition,2021,33(1):317-329.(in Chinese)
[20] 马秀花,桂瑞麒,焦娜,等.荞麦秸秆饲粮条件下甘露寡糖对滩羊瘤胃菌群结构的影响[J].动物营养学报,2021,33(4):2365-2377. MA X H,GUI R Q,JIAO N,et al.Effect of mannan oligosaccharides on rumen bacterial flora structure of Tan sheep under buckwheat straw diet condition[J].Chinese Journal of Animal Nutrition,2021,33(4):2365-2377.(in Chinese)
[21] HAN X F,LI B B,WANG X L,et al.Effect of dietary concentrate to forage ratios on ruminal bacterial and anaerobic fungal populations of cashmere goats[J].Anaerobe,2019,59:118-125.
[22] EL KAOUTARI A,ARMOUGOM F,GORDON J I,et al.The abundance and variety of carbohydrate-active enzymes in the human gut microbiota[J].Nature Reviews Microbiology,2013,11(7):497-504.  
[23] AN D D,DONG X Z,DONG Z Y.Prokaryote diversity in the rumen of yak (Bos grunniens) and Jinnan cattle (Bos taurus) estimated by 16S rDNA homology analyses[J].Anaerobe,2005,11(4):207-215.  
[24] MCCABE M S,CORMICAN P,KEOGH K,et al.Illumina MiSeq phylogenetic amplicon sequencing shows a large reduction of an uncharacterised succinivibrionaceae and an increase of the Methanobrevibacter gottschalkii clade in feed restricted cattle[J].PLoS One,2015,10(7):e0133234.
[25] 林静.活性干酵母对荷斯坦泌乳牛生产性能、瘤胃发酵和细菌群落的影响[D].硕士学位论文.乌鲁木齐:新疆农业大学,2017. LIN J.Effects of active dry yeast on production performance,ruminal fermentation and bacterial community of lactating Holstein dairy cow[D].Master's Thesis.Urumqi:Xinjiang Agricultural University,2017.(in Chinese)
[26] 程冠文.日粮碳水化合物类型调控瘤胃发酵乙/丙比的机制研究[D].硕士学位论文.泰安:山东农业大学,2019. CHENG G W.Study on the mechanism of dietary carbohydrate types regulating C2/C3 ratio in rumen fermentation[D].Master's Thesis.Tai'an:Shandong Agricultural University,2019.(in Chinese)
[27] JAMI E,ISRAEL A,KOTSER A,et al.Exploring the bovine rumen bacterial community from birth to adulthood[J].The ISME Journal,2013,7(6):1069-1079.  
[28] NEWBOLD C J,BROCK R,WALLACE R J.Influence of autoclaved or irradiated Aspergillus oryzae fermentation extract on fermentation in the rumen simulation technique (Rusitec)[J].Journal of Agricultural Science,1991,116(1):159-162.  
[29] KREHBIEL C R,BRITTON R A,HARMON D L,et al.The effects of ruminal acidosis on volatile fatty acid absorption and plasma activities of pancreatic enzymes in lambs[J].Journal of Animal Science,1995,73(10):3111-3121.  
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