研究论文 RESEARCH PAPER

胆固醇调节元件结合蛋白-1基因对高寒草原饲养西门塔尔牛血液脂肪酸组成的影响

  • 李新淼 ,
  • 王圆圆 ,
  • HESHUOTE Mailisi ,
  • 白玉廷 ,
  • 包音都古荣· ,
  • 金花 ,
  • 呼格吉勒图 ,
  • 敖日格勒 ,
  • 薛强
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  • 1. 内蒙古农业大学食品科学与工程学院, 呼和浩特 010018;
    2. 内蒙古自治区生物制造重点实验室, 呼和浩特 010018;
    3. 哥伦比亚大学, 纽约 10027, 美国;
    4. 内蒙古农业大学兽医学院, 呼和浩特 010018;
    5. 内蒙古贺斯格绿色产业进出口有限公司, 锡林郭勒 026321
李新淼(1997-),女,河北承德人,硕士,研究方向为食品机能学。E-mail:530327708@qq.com

收稿日期: 2021-06-04

  网络出版日期: 2022-01-18

基金资助

内蒙古自然科学基金项目(2017MS0357);内蒙古自治区科技计划项目(2018NC1377);内蒙古自治区科技重大专项项目(内财教[2013] 2186号);内蒙古自治区应用技术研究与开发资金计划(201803055);内蒙古自治区科技成果转化专项资金项目(2020CG0031);内蒙古自治区科技成果转化专项资金项目(内科发[2018] 88号);内蒙古草原英才项目(内财行[2021]333号)

Effects of Sterol-Regulatory Element Binding Protein-1 Gene on Blood Fatty Acid Composition of Simmental Cattle Raised in Alpine Grassland

  • LI Xinmiao ,
  • WANG Yuanyuan ,
  • HESHUOTE Mailisi ,
  • BAI Yuting ,
  • Baoyindugurong· ,
  • Jinhua ,
  • Hugejiletu ,
  • Aorigele ,
  • XUE Qiang
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  • 1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Inner Mongolia Key Laboratory of Bio-Manufacturing, Hohhot 010018, China;
    3. Columbia University, New York 10027, USA;
    4. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
    5. Inner Mongolia Hesige Green Industry Import and Export Co., Ltd., Xilingol 026321, China

Received date: 2021-06-04

  Online published: 2022-01-18

摘要

本试验旨在研究胆固醇调节元件结合蛋白-1(SREBP-1)基因对高寒草原饲养西门塔尔牛血液脂肪酸组成的影响,为其今后繁育中优良遗传基因的保留提供基础数据。试验使用血液样品来自高寒草原饲养的30头18月龄澳洲进口纯种西门塔尔公牛。利用气相色谱-质谱联用法测定血液脂肪酸组成和含量,实时荧光定量PCR测定血液SREBP-1基因相对表达量,并对SREBP-1基因进行测序。结果表明:高寒草原饲养的纯种西门塔尔牛血液共测得36种脂肪酸,其中含量较高的是棕榈酸(12.57%)、硬脂酸(21.76%)、油酸(22.11%)和亚油酸(29.53%)。血液SREBP-1基因相对表达量与棕榈酸、棕榈油酸、硬脂酸、油酸和单不饱和脂肪酸含量均呈正相关(P>0.05)。血液SREBP-1基因相对表达量对西门塔尔牛血液脂肪酸中的肉豆蔻酸、肉豆蔻油酸、棕榈酸、亚油酸和饱和脂肪酸(SFA)含量以及单不饱和脂肪酸/饱和脂肪酸(MUFA/SFA)、C14指数、C16指数、C18指数和伸长指数有显著性影响(P<0.05)。SREBP-1基因在第5内含子84 bp处有插入缺失多态性,表现为LL和LS基因型;SREBP-1基因的LL和LS基因型对脂肪酸组成均无显著性影响(P>0.05)。由此可见,SREBP-1基因可以作为高寒草原饲养下西门塔尔牛品种培育和评估的重要候选基因。

本文引用格式

李新淼 , 王圆圆 , HESHUOTE Mailisi , 白玉廷 , 包音都古荣· , 金花 , 呼格吉勒图 , 敖日格勒 , 薛强 . 胆固醇调节元件结合蛋白-1基因对高寒草原饲养西门塔尔牛血液脂肪酸组成的影响[J]. 动物营养学报, 2022 , 34(1) : 651 -658 . DOI: 10.3969/j.issn.1006-267x.2022.01.059

Abstract

This experiment was conducted to study the effects of sterol-regulatory element binding protein-1 (SREBP-1) gene on blood fatty acid composition of Simmental cattle raised in alpine grassland, and to provide a theoretical basis for the preservation of excellent genetic genes in the future process of breeding. Blood samples were collected from thirty 18-month-old alpine grasslands raised Australian imported Simmental bulls. The composition and content of fatty acids in blood were studied by gas chromatography-mass spectrometry method, the relative expression level of SREBP-1 gene was determined by real-time fluorescence quantitative PCR, and then the SREBP-1 gene was sequenced. The results showed as that 36 kinds of fatty acids were detected in the blood of pure Simmental cattle raised in alpine steppe, and the palmitic acid (12.57%), stearic acid (21.76%), oleic acid (22.11%) and linoleic acid (29.53%) contents were higher. The blood SREBP-1 gene relative expression level was positively correlated with palmitic acid, palmitoleic acid, stearic acid, oleic acid and monounsaturated fatty acid (P>0.05). The blood SREBP-1 gene relative expression level had significant effects on myristic acid, myristic acid, palmitic acid, linoleic acid and saturated fatty acids (SFA), as well as monounsaturated fatty acids/saturated fatty acids (MUFA/SFA), C14 index, C16 index, C18 index and elongation index in blood fatty acids of Simmental cattle. The SREBP-1 gene had insertion and deletion polymorphism at 84 bp of intron 5, which was characterized LL and LS genotypes. The LL and LS genotypes of SREBP-1 gene had no significant effects no fatty acid composition (P>0.05). In conclusion, the expression of SREBP-1 gene in blood of pure Simmental cattle raised in alpine grassland plays an irreplaceable role in regulating fatty acids. Therefore, the SREBP-1 gene can be used as an important candidate gene for breeding and evaluation of Simmental cattle breeds raised in alpine steppe.

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