研究论文 RESEARCH PAPER

山香圆叶提取物对文昌鸡生长性能、血清免疫和抗氧化功能以及肠道微生物的影响

  • 宋文静 ,
  • 宋琼莉 ,
  • 邹志恒 ,
  • 陈小连 ,
  • 刘林秀 ,
  • 谭佳 ,
  • 魏立民 ,
  • 熊平文 ,
  • 陶秀花 ,
  • 孙瑞萍 ,
  • 张艳
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  • 1. 海南省农业科学院畜牧兽医研究所, 海口 571100;
    2. 江西省农业科学院畜牧兽医研究所, 南昌 330200;
    3. 海南省热带动物繁育与疫病研究重点实验室, 海口 570203;
    4. 江西省农业科学院蔬菜花卉研究所, 南昌 330200
宋文静(1989-),女,河南安阳人,助理研究员,硕士,从事动物营养与饲料科学相关研究。E-mail:928152727@qq.com

收稿日期: 2021-12-28

  网络出版日期: 2022-07-14

基金资助

海南省热带动物繁育与疫病研究重点实验室开放课题(HKL20200201);国家肉鸡产业技术体系(CARS-41-Z17);江西省重点研发计划一般项目(20212BBF63025)

Effects of Turpiniae folium Extract on Growth Performance, Serum Immune and Antioxidant Function and Intestinal Microflora of Wenchang Chickens

  • SONG Wenjing ,
  • SONG Qiongli ,
  • ZOU Zhiheng ,
  • CHEN Xiaolian ,
  • LIU Linxiu ,
  • TAN Jia ,
  • WEI Limin ,
  • XIONG Pingwen ,
  • TAO Xiuhua ,
  • SUN Ruiping ,
  • ZHANG Yan
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  • 1. Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou 571100, China;
    2. Institute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China;
    3. Hainan Key Laboratory of Tropical Animal Breeding and Disease Research, Haikou 570203, China;
    4. Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China

Received date: 2021-12-28

  Online published: 2022-07-14

摘要

本试验旨在研究饲粮中添加山香圆叶提取物(TFE)对文昌鸡生长性能、血清免疫和抗氧化功能及肠道微生物的影响。选择同批次的42日龄雌性文昌鸡432羽,随机分为4组,每组6个重复,每个重复18羽。对照组饲喂基础饲粮,试验组饲喂在基础饲粮中分别添加0.015%、0.030%、0.060% TFE的试验饲粮,试验期为42 d。结果表明:1)随着饲粮中TFE添加水平的增加,文昌鸡终末体重呈显著线性升高(P<0.05),料重比(F/G)呈显著线性和二次曲线变化(P<0.05)。与对照组相比,添加0.030%和0.060%的TFE显著提高了文昌鸡的平均日增重(ADG)(P<0.05)。2)随着TFE添加水平的增加,文昌鸡血清总蛋白(TP)、球蛋白(GLB)、白细胞介素-10(IL-10)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量呈显著线性和二次曲线变化(P<0.05),血清免疫球蛋白M(IgM)含量呈显著线性增加(P<0.05)。与对照组相比,添加0.030%和0.060%的TFE显著提高了血清免疫球蛋白A(IgA)和免疫球蛋白G(IgG)含量(P<0.05),显著降低了血清丙二醛(MDA)、IL-6和TNF-α含量;添加0.060%的TFE显著提高了血清总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性和IL-10含量(P<0.05),不同添加水平的TFE均显著提高了文昌鸡血清谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05),显著降低了血清IL-1β含量(P<0.05)。3)随着TFE添加水平的增加,空肠绒毛高度和绒毛高度/隐窝深度呈显著线性和二次曲线变化(P<0.05)。4)与对照组相比,添加0.060%的TFE显著降低了盲肠变形菌门相对丰度(P<0.05),显著提高了粪肠杆菌属和乳酸杆菌属的相对丰度(P<0.05)。5)通过二次函数拟合,确定TFE最适添加水平为0.056%。综上所述,饲粮中添加TFE能够改善文昌鸡生长性能,提高文昌鸡血清免疫和抗氧化功能,可以通过提高血清抗炎因子含量,降低促炎因子含量,从而缓解机体炎症损伤,改善肠道组织形态,促进肠道对营养物质的吸收。本试验条件下,推荐42~84日龄文昌鸡TFE最适添加水平为0.056%。

本文引用格式

宋文静 , 宋琼莉 , 邹志恒 , 陈小连 , 刘林秀 , 谭佳 , 魏立民 , 熊平文 , 陶秀花 , 孙瑞萍 , 张艳 . 山香圆叶提取物对文昌鸡生长性能、血清免疫和抗氧化功能以及肠道微生物的影响[J]. 动物营养学报, 2022 , 34(7) : 4380 -4393 . DOI: 10.3969/j.issn.1006-267x.2022.07.029

Abstract

This experiment was conducted to investigate the effects of Turpiniae folium extract (TFE) on growth performance, serum immune and antioxidant function and intestinal microflora of Wenchang chickens. A total of 432 female Wenchang chickens at 42 days of age were randomly divided into 4 groups, with 6 replicates of 18 birds. Birds received either a basal diet (control group) or the basal diet+0.015%, 0.030% or 0.060% TFE. The experiment lasted for 42 days. The results showed as follows:1) the final body weight of chicken increased linearly (P<0.05), feed to gain ratio (F/G) changed linearly and quadratically (P<0.05) with increasing TFE levels. Compared with the control group, the average daily gain (ADG) of chicken significantly increased by adding 0.030% and 0.060% TFE (P<0.05). 2) With increasing TFE levels, dietary TFE supplementation resulted in linear and quadratic variations in serum total protein (TP), globulin (GLB), interleukin-10 (IL-10), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) contents (P<0.05), and linear increases in serum immunoglobulin M (IgM) content (P<0.05). Compared with the control group, serum immunoglobulin A (IgA) and immunoglobulin G (IgG) contents increased significantly (P<0.05), and malondialdehyde (MDA), IL-6 and TNF-α contents decreased significantly (P<0.05) in 0.030% and 0.060% TFE groups; the total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity and IL-10 content in serum showed significant increases in 0.060% TFE group (P<0.05); chicken fed TFE-supplemented diets exhibited both significant increases in glutathione peroxidase (GSH-Px) activity and decreases in serum IL-1β content (P<0.05). 3) Jejunum villus height (VH) and villus height/crypt depth (VH/CD) differed linearly and quadratically (P<0.05) with increase levels of dietary TFE. 4) Compared with the control group, the relative abundance of Proteobacteria in caecum decreased significantly (P<0.05), and the relative abundance of Faecalibacterium and Lactobacillus increased significantly (P<0.05) in 0.060% TFE group. 5) Analysis by quadratic regression showed the optimal supplementation TFE level was 0.056%. In conclusion, TFE can improve the growth performance, serum immune and antioxidant function of Wenchang chickens. Increasing the content of serum anti-inflammatory factors and reducing the content of pro-inflammatory factors can alleviate the body's inflammatory injury, improve the intestinal tissue morphology, and promote the intestinal absorption of nutrients. The optimal level of TFE supplementation for Wenchang chickens aged 42 to 84 d is 0.056%.

参考文献

[1] MEHDI Y,LÉTOURNEAU-MONTMINY M P,GAUCHER M L,et al.Use of antibiotics in broiler production:global impacts and alternatives[J].Animal Nutrition,2018,4(2):170-178.  
[2] 肖春荣,涂林锋,张睿增,等.山香圆属植物的化学成分及药理活性研究进展[J].中国中药杂志,2019,44(7):1295-1304. XIAO C R,TU L F,ZHANG R Z,et al.Research progress on chemical constituents and biological activities from Turpinia species[J].China Journal of Chinese Materia Medica,2019,44(7):1295-1304.(in Chinese)
[3] ZHAO L J,XIA B H,LIN L M,et al.Optimization of ultrasound-assisted enzymatic polysaccharide extraction from Turpiniae folium based on response surface methodology[J].Digital Chinese Medicine,2018,1(3):239-246.  
[4] 吴珊,李小芳,罗佳,等.大孔树脂纯化山香圆叶中的总黄酮[J].华西药学杂志,2014,29(6):680-682. WU S,LI X F,LUO J,et al.Purification of the total flavonoids from leaves of Turpinia arguta by macroporous resin[J].West China Journal of Pharmaceutical Sciences,2014,29(6):680-682.(in Chinese)
[5] 曾贤,陈世华,黄佩蓓,等.山香圆总黄酮提取工艺的研究[J].现代中药研究与实践,2015,29(5):46-48. ZENG X,CHEN S H,HUANG P B,et al.Study on the extraction of total flavones from Turpinia arguta Seem[J].Research and Practice on Chinese Medicines,2015,29(5):46-48.(in Chinese)
[6] 詹怡飞,张建军,毛友昌,等.山香圆含片抗菌作用的试验研究[J].江西中医学院学报,2005,17(2):55. ZHAN Y F,ZHANG J J,MAO Y C,et al.Experimental study on antibacterial effect of Turpinia arguta Seem buccal tablet[J].Journal of Jiangxi University of Traditional Chinese Medicine,2005,17(2):55.(in Chinese)
[7] 詹怡飞,张建军,陈娇婷,等.山香圆含片镇痛作用研究[J].时珍国医国药,2005,16(5):389-390. ZHAN Y F,ZHANG J J,CHEN J T,et al.Studies on the analgesia effect of Shanxiangyuan baccal tablet[J].Lishizhen Medicine and Materia Medica Research,2005,16(5):389-390.(in Chinese)
[8] 张磊,李俊,余世春,等.山香圆总黄酮对免疫功能低下小鼠免疫功能的影响[J].安徽医科大学学报,2006,41(5):539-543. ZHANG L,LI J,YU S C,et al.The effects of total flavonoids of Turpinia arguta Seen on the immune function of immunosuppressed mice induced by cyclophosphamide[J].Acta Universitatis Medicinalis Anhui,2006,41(5):539-543.(in Chinese)
[9] 张磊.山香圆总黄酮的抗炎免疫作用及部分机制研究[D].硕士学位论文.合肥:安徽医科大学,2003:23-24. ZHANG L.Anti-inflammatory and immunodulatory actions of total flavonoids of saciente and its mechanism[D].Master's Thesis.Hefei:Anhui Medical University,2003:23-24.(in Chinese)
[10] 张磊,李俊,余世春,等.山香圆总黄酮体外对大鼠佐剂性关节炎免疫功能的影响[J].中国药理学通报,2007,23(1):106-110. ZHANG L,LI J,YU S C,et al.Effect of total flavonoids of Turpinia arguta Seen on immune function in adjuvant arthritis rats in vitro[J].Chinese Pharmacological Bulletin,2007,23(1):106-110.(in Chinese)
[11] 王思佳.山香圆总黄酮抗慢性咽炎作用及其机制研究[D].硕士学位论文.南昌:江西中医药大学,2020. WANG S J.Anti-chronic pharyngitis effect of total flavonoids from Turpinia arguta Seem and its mechanism[D].Master's Thesis.Nanchang:Jiangxi University of Traditional Chinese Medicine,2020.(in Chinese)
[12] 海南省质量技术监督局.文昌鸡饲养管理技术规程:DB46/T 44-2011[S].海口:海南省质量技术监督局,2011. Hainan Provincial Quality and Technical Supervision Bureau.Technical regulations for breeding and management of Wenchang chickens:DB46/T 44-2011[S].Haikou:Hainan Provincial Quality and Technical Supervision Bureau,2011.(in Chinese)
[13] OTEIZA P I,FRAGA C G,MILLS D A,et al.Flavonoids and the gastrointestinal tract:local and systemic effects[J].Molecular Aspects of Medicine,2018,61:41-49.
[14] KAMBOH A A,ZHU W Y.Individual and combined effects of genistein and hesperidin supplementation on meat quality in meat-type broiler chickens[J].Journal of the Science of Food and Agriculture,2013,93(13):3362-3367.  
[15] DENLI M,OKAN F,ULUOCAK A N.Effect of dietary supplementation of herb essentialoils on the growthperformance,carcass and intestinal characteristics of quail(Coturnix coturnix japonica)[J].South African Journal of Animal Science,2004,34(3):174-179.
[16] 剧浩,郝小燕,刘森,等.饲粮中添加枸杞多糖对羔羊生长性能、屠宰性能和肉品质的影响[J].动物营养学报,2022,34(3):1777-1788. JU H,HAO X Y,LIU S,et al. Effects of dietary supplementation of lycium barbarum polysaccharide on growth performance,slaughter performance and meat quality of lambs[J].Chinese Journal of Animal Nutrition,2022,34(3):1777-1788.(in Chinese)
[17] APPELDOORN M M,VINCKEN J P,AURA A M,et al.Procyanidin dimers are metabolized by human microbiota with 2-(3,4-dihydroxyphenyl)acetic acid and 5-(3,4-dihydroxyphenyl)-gamma-valerolactone as the major metabolites[J].Journal of Agricultural and Food Chemistry,2009,57(3):1084-1092.  
[18] ZHANGL,WANGY J,LI D X,et al.The absorption,distribution,metabolism and excretion of procyanidins[J].Food & Function,2016,7(3):1273-1281.  
[19] EHRENSTEIN M R,NOTLEY C A.The importance of natural IgM:scavenger,protector and regulator[J].Nature Reviews Immunology,2010,10(11):778-786.  
[20] 吕天星,于晨龙,陈光明,等.2株乳酸菌对小鼠血清中IgG及IgA含量影响的研究[J].中国畜牧兽医,2014,41(6):117-120. LV T X,YU C L,CHEN G M,et al.Study on the influence of 2 strains of lactobacillus on the content of IgG and IgA in blood serum of mice[J].China Animal Husbandry & Veterinary Medicine,2014,41(6):117-120.(in Chinese)
[21] 张鹏飞.艾蒿水提物对脂多糖刺激的肉仔鸡免疫和抗氧化功能的影响及其机理研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2018:27-43. ZHANG P F.Effects of Artemisia argyiaqueous extract on immunological and anti-oxidative function in broilers exposedto lipopolysaccharide and the underlying mechanism[D].Ph.D.Thesis.Hohhot:Inner Mongolia Agricultural University,2018:27-43.(in Chinese)
[22] 王小宁,郑锦庆,伦海文,等.飞机草总黄酮对AA肉鸡生长性能、免疫器官指数和血清免疫球蛋白水平的影响[J].中兽医医药杂志,2016,35(2):18-23. WANG X N,ZHENG J Q,LUN H W,et al.Effects of total flavonoids extract from Eupatorium odoratum Linn.on growth performance,immune organ indexes and serum immunoglobulins level in AA broiler[J].Journal of Traditional Chinese Veterinary Medicine,2016,35(2):18-23.(in Chinese)
[23] 宋敏,王超普,邓盾,等.桑叶提取物对断奶仔猪生长性能、养分表观消化率、抗氧化能力和免疫功能的影响[J/OL].动物营养学报.https://kns.cnki.net/kcms/detail/11.5461.S.20220317.1143.066.html SONG M,WANG C P,DENG D,et al. Effects of mulberry leaf extract on growth performance, nutrient apparent digestibility,antioxidant ability and immune function of weaned piglets[J/OL].Chinese Journal of Animal Nutrition.https://kns.cnki.net/kcms/detail/11.5461.S.20220317.1143.066.html (in Chinese)
[24] 宫汝淳,田小东,欧小女,等.桑黄多糖对育成期蛋鸡生长性能、血液生化及免疫相关指标的影响[J].中国兽医杂志,2021,9(57):86-90. GONG R C,TIAN X D,OU X N,et al. Effects of Phellinus Linteus ploysaccharide on growth performance,hematological parameters and immunological indexes of growing-laying hens[J]. Chinese Journal of Veterinary Medicine,2021,9(57):86-90.(in Chinese)
[25] BENAVENTE-GARCÍA O,CASTILLO J.Update on uses and properties of citrus flavonoids:new findings in anticancer,cardiovascular,and anti-inflammatory activity[J].Journal of Agricultural Food Chemistry,2008,56(15):6185-6205.  
[26] ANDRÉ M C,GILLE C,GLEMSER P,et al.Bacterial reprogramming of PBMCs impairs monocyte phagocytosis and modulates adaptive T cell responses[J].Journal of Leukocyte Biology,2012,91(6):977-989.  
[27] WU Q J,WANG Y Q,QI Y X.Influence of procyanidin supplementation on the immune responses of broilers challenged with lipopolysaccharide[J].Animal Science Journal,2017,88(7):983-990.  
[28] 赵亚波,张艳梅,白晨,等.葱属植物提取物对动物的抗炎作用及其机理[J].饲料工业,2021,42(17):37-42. ZHAO Y B,ZHANG Y M,BAI C,et al.Anti-inflammatory effect and mechanism of Allium plant extracts on animals[J].Feed Industry,2021,42(17):37-42.(in Chinese)
[29] FRIDOVICH I.Superoxide radical and superoxide dismutases[J].Annual Review of Biochemistry,1995,64:97-112.
[30] 丘富安,张建龙,丁育诗,等.车前子黄酮的响应面法优化微波提取及其对鸡抗氧化能力的影响[J].中国兽医科学,2017,47(10):1315-1325. QIU F A,ZHANG J L,DING Y S,et al.Optimization of microwave-assisted extraction of plantain seed flavonoids by response surface methodology and its effects on antioxidant activities in chickens response to oxidative stress[J].Chinese Veterinary Science,2017,47(10):1315-1325.(in Chinese)
[31] 贾琦珍,陈根元,王帅,等.小花棘豆黄酮对热应激肉仔鸡抗氧化能力的影响[J].中国饲料,2014(17):14-17. JIA Q Z,CHEN G Y,WANG S,et al.Effects of total flavonoids in Oxytiopis glabra DC on antioxidative ability in broilers by heat stress[J].China Feed,2014(17):14-17.(in Chinese)
[32] 谭榀新,叶涛,刘湘新,等.植物提取物抗氧化成分及机理研究进展[J].食品科学,2010,31(15):288-292. TAN P X,YE T,LIU X X,et al.Research advances in antioxidant composition of botanical extracts and their action mechanisms[J].Food Science,2010,31(15):288-292.(in Chinese)
[33] 史东辉,马学会.植物提取物的抗氧化作用及其在养禽生产中的应用研究进展[J].中国畜牧杂志,2009,45(19):73-76. SHI D H,MA X H.Advances in the antioxidative effect of plant extracts and the application to poultry production[J].Chinese Journal of Animal Science,2009,45(19):73-76.(in Chinese)
[34] CSERNUS B,CZEGLÉDI L.Physiological,antimicrobial,intestine morphological,and immunological effects of fructooligosaccharides in pigs[J].Archives Animal Breeding,2020,63(2):325-335.  
[35] 刘靖.博落回生物碱对黄羽肉鸡生长的影响[D].硕士学位论文.长沙:湖南农业大学,2010:21-30. LIU J.The effect of macleaya alkaloids on the growth of yellow broilers[D].Master's Thesis.Changsha:Hunan Agricultural University,2010:21-30.(in Chinese)
[36] 岳进,杨贵芸,邓云,等.植物提取物抗菌作用及其在食品中的应用[J].上海交通大学学报(农业科学版),2013,31(5):36-42,53. YUE J,YANG G Y,DENG Y,et al.Research advances in antimicrobial action of plant extracts and their applications in food[J].Journal of Shanghai Jiaotong University(Agricultural Science),2013,31(5):36-42,53.(in Chinese)
[37] 符莎露,吴甜甜,吴春华,等.植物多酚的抗氧化和抗菌机理及其在食品中的应用[J].食品工业,2016,37(6):242-246. FU S L,WU T T,WU C H,et al.Antioxidation and antibacterial mechanisms of plant polyphenols and application in food industry[J].The Food Industry,2016,37(6):242-246.(in Chinese)
[38] MORENO S,SCHEYER T,ROMANO CS,et al.Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition[J].Free Radical Research,2006,40(2):223-231.  
[39] 黄桂荣,杜晓英,徐大高,等.三峡地区20种植物甲醇提取物的抗真菌活性[J].华南农业大学学报,2007,28(3):37-41. HUANG G R,DU X Y,XU D G,et al.Antifungal Activities in methanol extracts from twenty species of plants in Three Gorges region,Hubei Province[J].Journal of South China Agricultural University,2007,28(3):37-41.(in Chinese)
[40] HAMMER K A,CARSON C F,RILEY T V.Antimicrobial activity of essential oils and other plant extracts[J].Journal of Applied Microbiology,1999,86(6):985-990.  
[41] SARMIENTO-RUBIANO L A,ZÚÑIGA M,PÉREZ-MARTÍNEZ G,et al.Dietary supplementation with sorbitol results in selective enrichment of lactobacilli in rat intestine[J].Research in Microbiology,2007,158(8/9):694-701.
[42] HAO Y,LI D F,PIAO X L,et al.Forsythia suspensa extract alleviates hypersensitivity induced by soybean β-conglycinin in weaned piglets[J].Journal of Ethnopharmacology,2010,128(2):412-418.  
[43] TABASCO R,SÁNCHEZ-PATÁN F,MONAGAS M,et al.Effect of grape polyphenols on lactic acid bacteria and Bifidobacteria growth:resistance and metabolism[J].Food Microbiology,2011,28(7):1345-1352.  
[44] DURLU-ÖZKAYA F,AYHAN K,VURAL N.Biogenic amines produced by Enterobacteriaceae isolated from meat products[J].Meat Science,2001,58(2):163-166.  
[45] 乔树民.与变形杆菌联系的爆发性食物中毒的流行病学调查和实验研究[J].中华医学杂志,1957,43(8):607-613. QIAO S M.Epidemiological investigation and experimental study of explosive food poisoning associated with proteus Bacillus[J].National Medical Journal of China,1957,43(8):607-613.(in Chinese)
[46] MUKHOPADHYA I,HANSEN R,EL-OMAR E M,et al.IBD-what role do Proteobacteria play?[J].Nature Reviews.Gastroenterology & Hepatology,2012,9(4):219-230.  
[47] QUÉVRAIN E,MAUBERT M A,MICHON C,et al.Identification of an anti-inflammatory protein from Faecalibacterium prausnitzii,a commensal bacterium deficient in Crohn's disease[J].Gut,2016,65(3):415-425.  
[48] ZHOU L X,ZHANG M M,WANG Y M,et al.Faecalibacterium prausnitzii produces butyrate to maintain Th17/Treg balance and to ameliorate colorectal colitis by inhibiting histone deacetylase 1[J].Inflammatory Bowel Diseases,2018,24(9):1926-1940.  
[49] DEEPAK V,SUNDAR W A,PANDIAN S R K,et al.Exopolysaccharides from Lactobacillus acidophilus modulates the antioxidant status of 1,2-dimethyl hydrazine-induced colon cancer rat model[J].3 Biotech,2021,11(5):225.
[50] ANGELAKIS E,RAOULT D.The increase of Lactobacillus species in the gut flora of newborn broiler chicks and ducks is associated with weight gain[J].PLoS One,2010,5(5):e10463.
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