动物营养学报 >

2022 , Vol. 34 >Issue 7: 4562 - 4571

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.07.045

研究论文 RESEARCH PAPER

菌酶联用制剂对滩羊生长性能、养分表观消化率、瘤胃发酵参数及血清生化指标的影响

  • 张腱皓 ,
  • 钟锐 ,
  • 李荫柱 ,
  • 李海海 ,
  • 雷晓青 ,
  • 寇启芳 ,
  • 张桂杰
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  • 1. 宁夏大学农学院, 银川 750021;
    2. 宁夏草牧业工程技术研究中心, 银川 750021;
    3. 天津云力之星生物科技有限公司, 天津 300354;
    4. 吴忠市红寺堡区天源良种羊繁育养殖有限公司, 吴忠 751900
张腱皓(2000-),男,河南辉县人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:1179051152@qq.com

收稿日期: 2022-01-10

  网络出版日期: 2022-07-14

基金资助

国家自然科学基金项目（31960672）；农牧交错带牛羊牧繁农育关键技术集成示范项目（16200158-08）；宁夏高等学校一流学科建设（草学学科）资助项目（NXYLXK2017A01）；滩羊大理石花纹（雪花）肉生产关键技术研究与集成示范（2019BEF02001）

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Effects of Bacteria-Enzyme Combined Preparation on Growth Performance, Nutrient Apparent Digestibility, Rumen Fermentation Parameters and Serum Biochemical Indexes of Tan Sheep

  • ZHANG Jianhao ,
  • ZHONG Rui ,
  • LI Yinzhu ,
  • LI Haihai ,
  • LEI Xiaoqing ,
  • KOU Qifang ,
  • ZHANG Guijie
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  • 1. Agricultural college of Ningxia University, Yinchuan 750021, China;
    2. Ningxia Grassland and Animal Husbandry Engineering Technology Research Center, Yinchuan 750021, China;
    3. Winning of Tianjin Biotechnology Industry Company, Tianjin 300354, China;
    4. Hongsibu District Tianyuan Well-Bred Sheep Breeding Co., Ltd., Wuzhong 751900, China

Received date: 2022-01-10

  Online published: 2022-07-14

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摘要

本试验旨在研究菌酶联用制剂对滩羊生长性能、养分表观消化率、瘤胃发酵参数及血清生化指标的影响。选用4月龄左右、体重[（19.12±1.83）kg]相近的健康去势滩羔羊48只，随机分为4组，每组3个重复，每个重复4只羊。各组分别在基础饲粮中添加0（对照组）、0.05%、0.10%、0.15%的菌酶联用制剂（酵母菌1.0×1010 CFU/g、纤维素酶10 000 IU/g、木聚糖酶1 000 IU/g和甘露聚糖酶600 IU/g）。预试期14 d，正试期134 d。结果表明：1）与对照组相比，饲粮中添加0.05%、0.10%、0.15%菌酶联用制剂显著增加了滩羊平均日采食量和终末体重（P<0.05）；饲粮中添加0.10%和0.15%菌酶联用制剂显著增加了滩羊平均日增重（P<0.05），显著降低了料重比（P<0.05）。2）与对照组相比，饲粮中添加0.05%、0.10%、0.15%菌酶联用制剂显著增加了饲粮干物质、有机物、中性洗涤纤维表观消化率（P<0.05），饲粮中添加0.10%和0.15%菌酶联用制剂显著增加了饲粮粗蛋白质表观消化率（P<0.05）。3）与对照组相比，饲粮中添加0.05%、0.10%、0.15%菌酶联用制剂极显著增加了瘤胃液挥发性脂肪酸、乙酸和丙酸含量（P<0.01），显著增加了瘤胃液戊酸含量（P<0.05），显著降低了瘤胃液氨态氮含量和乙酸/丙酸（P<0.05）；饲粮中添加0.10%和0.15%菌酶联用制剂显著增加了瘤胃液丁酸含量（P<0.05），显著降低了瘤胃液异戊酸含量（P<0.05）。4）与对照组相比，饲粮中添加0.05%、0.10%和0.15%菌酶联用制剂显著增加了血清总蛋白、白蛋白、球蛋白和免疫球蛋白G含量（P<0.05）；饲粮中添加0.10%和0.15%菌酶联用制剂显著降低了血清尿素氮和肌酐含量（P<0.05），显著增加了血清葡萄糖和甘油三酯含量（P<0.05），极显著增加了血清免疫球蛋白M含量（P<0.01）。综上所述，饲粮中添加菌酶联用制剂能够提高滩羊饲粮养分表观消化率，改善瘤胃发酵参数和血清生化指标，进而改善滩羊生长性能，菌酶联用制剂添加量以0.10%为宜。

关键词： 菌酶联用; 滩羊; 生长性能; 表观消化率; 瘤胃发酵参数

本文引用格式

张腱皓 , 钟锐 , 李荫柱 , 李海海 , 雷晓青 , 寇启芳 , 张桂杰 . 菌酶联用制剂对滩羊生长性能、养分表观消化率、瘤胃发酵参数及血清生化指标的影响[J]. 动物营养学报, 2022 , 34(7) : 4562 -4571 . DOI: 10.3969/j.issn.1006-267x.2022.07.045

Abstract

This experiment was conducted to study the effects of bacteria-enzyme combined preparation on growth performance, nutrient apparent digestibility, rumen fermentation parameters and serum biochemical indexes of Tan sheep. Forty-eight 4-month-old healthy castrated beach lambs with similar body weight[(19.12±1.83) kg] were randomly divided into 4 groups with 3 replicates in each group and 4 lambs in each replicate. Sheep in 4 groups were fed basal diets supplemented 0 (control group), 0.05%, 0.10% and 0.15% bacteria-enzyme combined preparation (active yeast 1.0×1010 CFU/g, cellulase 10 000 IU/g, xylanase 1 000 IU/g and mannanase 600 IU/g), respectively. The pre-test period lasted for 14 days, and the experimental period lasted for 134 days. The results showed as follows:1) compared with the control group, dietary supplemented 0.05%, 0.10% and 0.15% bacteria-enzyme combined preparation significantly increased the final body weight and average daily feed intake of Tan sheep (P<0.05); dietary supplemented 0.10% and 0.15% bacteria-enzyme combined preparation significantly increased the average daily gain of Tan sheep (P<0.05), and significantly decreased the feed to gain ratio (P<0.05). 2) Compared with the control group, dietary supplemented 0.05%, 0.10% and 0.15% bacteria-enzyme combined preparation significantly increased the apparent digestibility of dry matter, organic matter and neutral detergent fiber in diet (P<0.05), dietary supplemented 0.10% and 0.15% bacteria-enzyme combined preparation significantly increased the apparent digestibility of crude protein in diet (P<0.05). 3) Compared with the control group, dietary supplemented 0.05%, 0.10% and 0.15% bacteria-enzyme combined preparation significantly increased the contents of volatile fatty acids, acetic acid and propionic acid in rumen fluid (P<0.01), significantly increased the rumen fluid valeric acid content (P<0.05), and significantly decreased the ammonia nitrogen content and acetic acid/propionic acid in rumen fluid (P<0.05); dietary supplemented 0.10% and 0.15% bacteria-enzyme combined preparation significantly increased the rumen fluid butyric acid content (P<0.05), and significantly decreased the rumen fluid isovaleric acid content (P<0.05). 4) Compared with the control group, dietary supplemented 0.05%, 0.10% and 0.15% bacteria-enzyme combined preparation significantly increased the contents of total protein, albumin, globulin and immunoglobulin G in serum (P<0.05); dietary supplemented 0.10% and 0.15% bacteria-enzyme combined preparation significantly decreased the contents of urea nitrogen and creatinine in serum (P<0.05), significantly increased the contents of glucose and triglycerides in serum (P<0.05), and significantly increased the serum immunoglobulin M content (P<0.01). In conclusion, dietary supplementation of bacterium-enzyme combined preparation can increase the nutrient apparent digestibility, improve the rumen fermentation parameters and serum biochemical indexes, and further improve the growth performance of Tan sheep. The optimal addition amount of bacteria-enzyme combined preparation is 0.10%.

Key words： bacteria-enzyme combined; Tan sheep; growth performance; apparent digestibility; rumen fermentation parameters

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