研究论文 RESEARCH PAPER

生物蛋白API对肉兔生长性能和肠道健康的影响

  • 张莉 ,
  • 汤加勇 ,
  • 贾刚 ,
  • 田刚 ,
  • 刘光芒 ,
  • 陈小玲 ,
  • 蔡景义 ,
  • 赵华
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  • 四川农业大学动物营养研究所, 动物抗病营养教育部重点实验室, 农业农村部动物抗病营养与饲料重点实验室, 动物抗病营养四川省重点实验室, 成都 611130
张莉(1995-),女,四川宜宾人,硕士研究生,从事饲料资源开发与利用研究。E-mail:zhangli644211@126.com

收稿日期: 2022-01-13

  网络出版日期: 2022-07-14

基金资助

四川农业大学双支计划(03570126);四川吉隆达生物科技有限公司项目(2122319014)

Effects of Bioengineering Protein API on Growth Performance and Intestinal Health of Meat Rabbits

  • ZHANG Li ,
  • TANG Jiayong ,
  • JIA Gang ,
  • TIAN Gang ,
  • LIU Guangmang ,
  • CHEN Xiaoling ,
  • CAI Jingyi ,
  • ZHAO Hua
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  • Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Key Laboratory of Animal Disease-Resistant Nutrition and Feed of Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2022-01-13

  Online published: 2022-07-14

摘要

本试验旨在研究饲粮中添加不同水平生物蛋白API(含鲶鱼抗菌肽parasin I功能片段的基因工程蛋白)对肉兔生长性能和肠道健康的影响。选取30日龄健康且体重[(644.93±31.92)g]相近的断奶伊拉商品肉兔144只,随机分为4组,每组18个重复,每个重复2只兔。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加0.1、0.5和1.0 mg/kg API。试验期8周。结果表明:1)饲粮中添加API对肉兔末重、平均日采食量(ADFI)、平均日增重(ADG)和料重比(F/G)无显著影响(P>0.05)。与对照组相比,饲粮中添加1.0 mg/kg API可降低肉兔腹泻率、死亡率和健康风险指数。2)与对照组相比,饲粮中添加1.0 mg/kg API可显著降低盲肠大肠杆菌数量(P<0.05),并显著提高盲肠丙酸比例(P<0.05)。3)与对照组相比,饲粮中添加1.0 mg/kg API可显著提高空肠封闭蛋白-1(Claudin-1)的mRNA相对表达量(P<0.05),饲粮中添加0.5和1.0 mg/kg API可显著提高空肠白细胞介素-2(IL-2)的mRNA相对表达量(P<0.05)。4)与对照组相比,饲粮中添加1.0 mg/kg API可显著提高空肠淀粉酶活性(P<0.05),饲粮中添加0.1、0.5和1.0 mg/kg API均可显著提高空肠脂肪酶和胰蛋白酶活性(P<0.05)。综上所述,饲粮中添加API可降低肉兔腹泻率、死亡率和盲肠大肠杆菌数量,提高空肠Claudin-1和IL-2的mRNA相对表达量,提高空肠消化酶活性,表明API可提高肉兔的消化能力和改善肠道屏障,从而促进肠道健康。综合来看,本试验饲粮中API的适宜添加水平为1.0 mg/kg。

本文引用格式

张莉 , 汤加勇 , 贾刚 , 田刚 , 刘光芒 , 陈小玲 , 蔡景义 , 赵华 . 生物蛋白API对肉兔生长性能和肠道健康的影响[J]. 动物营养学报, 2022 , 34(7) : 4696 -4703 . DOI: 10.3969/j.issn.1006-267x.2022.07.056

Abstract

This experiment was conducted to evaluate the effects of supplementing bioengineering protein API (an engineered protein contained catfish antimicrobial peptide parasin I fragment) on growth performance and intestinal health of meat rabbits. A total of 144 healthy 30-day-old weaned meat rabbits with similar body weight[(644.93±31.92) g] were randomly assigned into 4 groups with 18 replicates per group and 2 rabbits per replicate. Rabbits in the control group were fed a basal diet, and others in experimental groups were fed basal diets supplemented with 0.1, 0.5 and 1.0 mg/kg API. The experiment lasted for 8 weeks. The results showed as follows:1) dietary API supplementation had no significant effects on the final weight, average daily feed intake (ADFI), average daily gain (ADG) and feed to weight ratio (F/G) of meat rabbits (P>0.05). Compared with the control group, dietary supplemented with 1.0 mg/kg API reduced the diarrhea rate, mortality rate and health risk index. 2) Compared with the control group, dietary supplemented with 1.0 mg/kg API significantly decreased the cecal Escherichia coli number (P<0.05), and significantly increased the cecal propionic acid ratio (P<0.05). 3) Compared with the control group, dietary supplemented with 1.0 mg/kg API significantly increased the jejunal Claudin-1 mRNA relative expression level (P<0.05), and dietary supplemented with 0.5 and 1.0 mg/kg API significantly increased the jejunal interleukin-2 (IL-2) mRNA relative expression level (P<0.05). 4) Compared with the control group, dietary supplemented with 1.0 mg/kg API significantly increased the jejunal amylase activity (P<0.05), and dietary supplemented with 0.1, 0.5 and 1.0 mg/kg API significantly increased the jejunal lipase and trypsin activities (P<0.05). In summary, dietary API supplementation reduces the diarrhea rate, mortality rate and cecal Escherichia coli number of meat rabbits, increases the mRNA relative expression levels of Claudin-1 and IL-2 and activity of digestive enzyme in jejunum, which shows API can improve the digestive capacity and intestinal barrier of the rabbits, then enhances the intestinal health. In general, the suitable dietary API supplemental level is 1.0 mg/kg in this experiment.

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