动物营养学报 >

2022 , Vol. 34 >Issue 7: 4704 - 4715

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.07.057

研究论文 RESEARCH PAPER

21日龄仔猪断奶过程不同肠段细胞外基质重要蛋白表达规律的研究

  • 唐青松 ,
  • 王丽 ,
  • 徐娥 ,
  • 刘春艳 ,
  • 肖明飞 ,
  • 肖昊 ,
  • 吴绮雯 ,
  • 蒋宗勇 ,
  • 易宏波
展开
  • 1. 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业农村部华南动物营养与饲料重点实验室, 岭南现代农业科学与技术广东省实验室茂名分中心, 广东省畜禽育种与营养研究重点实验室, 广州 510640;
    2. 贵州大学动物科学学院, 动物营养与饲料研究所, 贵阳 550025
唐青松(1996-),男,贵州盘州人,硕士研究生,动物营养与饲料科学专业。E-mail:tqs5213@foxmail.com

收稿日期: 2022-01-08

  网络出版日期: 2022-07-14

基金资助

财政部和农业农村部国家现代农业产业技术体系；科技创新战略专项资金（高水平农科院建设）（R2016YJ-YB2003，R2019PY-QF005，202106TD）；广东省现代农业产业技术体系生猪创新团队项目（2021KJ126）；茂名实验室自主科研项目（2021ZZ003）

收起

Study on Expression Regularity of Important Proteins in Extracellular Matrix of Different Intestinal Segments during Weaning of 21-Day-Old Piglets

  • TANG Qingsong ,
  • WANG Li ,
  • XU E ,
  • LIU Chunyan ,
  • XIAO Mingfei ,
  • XIAO Hao ,
  • WU Qiwen ,
  • JIANG Zongyong ,
  • YI Hongbo
Expand
  • 1. Guangdong Key Laboratory of Animal Breeding and Nutrition, Maoming Branch of Guangdong Laboratory for Lingnan Modern Agriculture Science and Technology, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Institute of Animal Nutrition and Feed Science, College of Animal Science, Guizhou University, Guiyang 550025, China

Received date: 2022-01-08

  Online published: 2022-07-14

Fold

摘要

本试验旨在研究21日龄仔猪断奶过程不同肠段细胞外基质（ECM）重要蛋白的表达规律。选用24头21日龄的断奶仔猪随机分配到6个栏中，分别于断奶后0、7、14和28 d每栏随机选择1头仔猪进行麻醉后处死，采集十二指肠、空肠和回肠样品，测定ECM相关基因和蛋白的表达量。结果表明：1）与0 d相比，7、14和28 d十二指肠Ⅰ型胶原蛋白α2链（COL1A2）、Ⅲ型胶原蛋白α1链（COL3A1）、Ⅳ型胶原蛋白α2链（COL4A2）、Ⅴ型胶原蛋白α1链（COL5A1）、Ⅵ型胶原蛋白α1链（COL6A1）、纤维黏连蛋白1（FN1）、肌腱蛋白C（TNC）和整合素α1（ITGA1）的mRNA相对表达量显著降低（P<0.05），且14 d十二指肠COL1A2和FN1的mRNA相对表达量显著低于28 d（P<0.05），14 d十二指肠COL3A1的mRNA相对表达量显著低于7和28 d（P<0.05），7 d十二指肠整合素β2（ITGB2）的mRNA相对表达量显著低于14和28 d（P<0.05）。2）与0 d相比，7、14和28 d空肠COL1A2、COL3A1、COL4A2和ITGA1的mRNA相对表达量显著降低（P<0.05），且7 d空肠COL5A1和COL6A1的mRNA相对表达量显著低于0和28 d（P<0.05），7 d空肠TNC和ITGB2的mRNA相对表达量显著低于28 d（P<0.05）。3）7 d回肠FN1的mRNA相对表达量显著低于0、14和28 d（P<0.05）。4）与0 d相比，7、14和28 d空肠COL1A2和Ⅵ型胶原蛋白的蛋白相对表达量显著降低（P<0.05），14和28 d空肠COL4A2、层黏连蛋白和FN1的蛋白相对表达量显著降低（P<0.05）。综上所述，仔猪断奶后十二指肠和空肠的胶原蛋白（COL1A2、COL3A1、COL4A2、COL5A1、COL6A1和Ⅵ型胶原蛋白）、FN1、层黏连蛋白、TNC、ITGA1和ITGB2的表达和分泌降低；十二指肠COL1A2、COL3A1和FN1的mRNA相对表达量在断奶后14 d降到最低，空肠COL5A1和COL6A1的mRNA相对表达量在断奶后7 d降到最低；仔猪断奶后7 d回肠FN1的mRNA相对表达量显著降低，但断奶对其他ECM相关基因表达无显著影响。

关键词： 断奶仔猪; 细胞外基质; 胶原蛋白; 表达规律

本文引用格式

唐青松 , 王丽 , 徐娥 , 刘春艳 , 肖明飞 , 肖昊 , 吴绮雯 , 蒋宗勇 , 易宏波 . 21日龄仔猪断奶过程不同肠段细胞外基质重要蛋白表达规律的研究[J]. 动物营养学报, 2022 , 34(7) : 4704 -4715 . DOI: 10.3969/j.issn.1006-267x.2022.07.057

Abstract

The purpose of this experiment was to study the expression regularity of important proteins in the extracellular matrix (ECM) of different intestinal segments during weaning of 21-day-old piglets. Twenty-four piglets weaned at 21 days of age were randomly assigned into six pens. One pig was randomly selected from each pen at 0, 7, 14 and 28 days after weaned and executed after anesthesia, and then the samples of duodenum, jejunum and ileum were collected to determine the expression levels of ECM related genes and proteins. The results showed as follows:1) compared with 0 d, the mRNA relative expression levels of type Ⅰ collagen α2 chain (COL1A2), type Ⅲ collagen α1 chain (COL3A1), type Ⅳ collagen α2 chain (COL4A2), type Ⅴ collagen α1 chain (COL5A1), type Ⅵ collagen α1 chain (COL6A1), fibronectin 1 (FN1), tenascin C (TNC) and integrin α1 (ITGA1) in duodenum at 7, 14 and 28 d were significantly decreased (P<0.05). Moreover, the mRNA relative expression levels of COL1A2 and FN1 in duodenum were significantly decreased at 14 d compared with 28 d (P<0.05), and the COL3A1 mRNA relative expression level in duodenum was significantly lower at 14 d than that at 7 and 28 d (P<0.05), and the integrin β2 (ITGB2) mRNA relative expression level in duodenum at 7 d was lower than that at 14 and 28 d (P<0.05). 2) Compared with 0 d, the mRNA relative expression levels of COL1A2, COL3A1, COL4A2, and ITGA1 in jejunum at 7, 14 and 28 d were significantly decreased (P<0.05). Additionally, the mRNA relative expression levels of COL5A1 and COL6A1 in jejunum were significantly lower at 7 d than those at 0 and 28 d (P<0.05), and the mRNA relative expression levels of TNC and ITGB2 in jejunum at 7 d were significantly lower than that at 28 d (P<0.05). 3) The FN1 mRNA relative expression level in ileum at 7 d was significantly lower than that at 0, 14 and 28 d (P<0.05). 4) Compared with 0 d, the protein relative expression levels of COL1A2 and type Ⅵ collagen in jejunum at 7, 14 and 28 d were significantly decreased (P<0.05), and the protein relative expression levels of COL4A2, laminin and FN1 in jejunum at 14 and 28 d were significantly decreased (P<0.05). In conclusion, the expression and secretion of collagen (COL1A2, COL3A1, COL4A2, COL5A1, COL6A1 and type Ⅵ collagen), FN1, laminin, TNC, ITGA1 and ITGB2 are decreased in duodenum and jejunum of piglets after weaning; the mRNA relative expression levels of COL1A2, COL3A1 and FN1 in duodenum are the lowest at 14 d after weaning, and the mRNA relative expression levels of COL5A1 and COL6A1 in jejunum are the lowest at 7 d after weaning; the FN1 mRNA relative expression in ileum at 7 d after weaning is significantly decreased, but weaning has no significant effect on the expression of other ECM-related genes.

Key words： weaned piglets; extracellular matrix; collagen; expression regularity

参考文献

[1] XIONG X,TAN B,SONG M,et al.Nutritional intervention for the intestinal development and health of weaned pigs[J].Frontiers in Veterinary Science,2019,6:46.
[2] THEOCHARIS A D,SKANDALIS S S,GIALELI C,et al.Extracellular matrix structure[J].Advanced Drug Delivery Reviews,2016,97:4-27.
[3] GELSE K,PÖSCHL E,AIGNER T.Collagens-structure,function,and biosynthesis[J].Advanced Drug Delivery Reviews,2003,55(12):1531-1546.  
[4] PETERSON R J,KOVAL M.Above the matrix:functional roles for apically localized integrins[J].Frontiers in Cell and Developmental Biology,2021,9:699407.
[5] ENGIN A B,NIKITOVIC D,NEAGU M,et al.Mechanistic understanding of nanoparticles' interactions with extracellular matrix:the cell and immune system[J].Particle and Fibre Toxicology,2017,14(1):22.
[6] HYNES R O.The extracellular matrix:not just pretty fibrils[J].Science,2009,326(5957):1216-1219.  
[7] TOMLIN H,PICCININI A M.A complex interplay between the extracellular matrix and the innate immune response to microbial pathogens[J].Immunology,2018,155(2):186-201.  
[8] 黎林峰.秀丽隐杆线虫上皮细胞机械支撑结构调控自噬机制研究[D].硕士学位论文.苏州:苏州大学,2017. LI L F.Spatially-restricted activation of autophagy in the C. elegans epidermis upon tissue damage[D].Master's Thesis.Suzhou:Soochow University,2017.(in Chinese)
[9] KISS M,KISS A A,RADICS M,et al.Drosophila type Ⅳ collagen mutation associates with immune system activation and intestinal dysfunction[J].Matrix Biology,2016,49:120-131.
[10] LIU C C,LIN S P,HSU H S,et al.Erratum:suspension survival mediated by PP2A-STAT3-Col ⅩⅦ determines tumour initiation and metastasis in cancer stem cells[J].Nature Communications,2016,7:14027.
[11] DE ARCANGELIS A,HAMADE H,ALPY F,et al.Hemidesmosome integrity protects the colon against colitis and colorectal cancer[J].Gut,2017,66(10):1748-1760.  
[12] LIU C C,LIN S P,HSU H S,et al.Suspension survival mediated by PP2A-STAT3-Col ⅩⅦ determines tumour initiation and metastasis in cancer stem cells[J].Nature Communications,2016,7:11798.
[13] MOHAN V,DAS A,SAGI I.Emerging roles of ECM remodeling processes in cancer[J].Seminars in Cancer Biology,2020,62:192-200.
[14] MONTELEONE I,ZORZI F,MARAFINI I,et al.Aryl hydrocarbon receptor-driven signals inhibit collagen synthesis in the gut[J].European Journal of Immunology,2016,46(4):1047-1057.  
[15] BEJARANO L,JORDÃO M J C,JOYCE J A.Therapeutic targeting of the tumor microenvironment[J].Cancer Discovery,2021,11(4):933-959.  
[16] CHEN Y,KIM J,YANG S J,et al.Type Ⅰ collagen deletion in αSMA+ myofibroblasts augments immune suppression and accelerates progression of pancreatic cancer[J].Cancer Cell,2021,39(4):548-565.e6.  
[17] DI LULLO G A,SWEENEY S M,KORKKO J,et al.Mapping the ligand-binding sites and disease-associated mutations on the most abundant protein in the human,type Ⅰcollagen[J].Journal of Biological Chemistry,2002,277(6):4223-4231.  
[18] KADLER K E,BALDOCK C,BELLA J,et al.Collagens at a glance[J].Journal of Cell Science,2007,120(12):1955-1958.  
[19] RICARD-BLUM S.The collagen family[J].Cold Spring Harbor Perspectives in Biology,2011,3(1):a004978.
[20] 唐青松,徐娥,王丽,等.胶原蛋白动态平衡及其对动物肠道健康的作用[J].动物营养学报,2020,32(12):5578-5586. TANG Q S,XU E,WANG L,et al.Collagen homeostasis and its effects on intestinal health of animal[J].Chinese Journal of Animal Nutrition,2020,32(12):5578-5586.(in Chinese)
[21] MADEC F,BRIDOUX N,BOUNAIX S,et al.Measurement of digestive disorders in the piglet at weaning and related risk factors[J].Preventive Veterinary Medicine,1998,35(1):53-72.  
[22] LI Z H,CHEN S W,CUI H W,et al.Tenascin-C-mediated suppression of extracellular matrix adhesion force promotes entheseal new bone formation through activation of Hippo signalling in ankylosing spondylitis[J].Annals of the Rheumatic Diseases,2021,80(7):891-902.  
[23] VAN MECHELEN M,LORIES R.Tenascin-C,a novel target to inhibit new bone formation in axial spondyloarthritis,linked with inflammation,mechanical strain and tissue damage[J].Annals of the Rheumatic Diseases,2021,80(7):823-824.  
[24] SONG J,ZHAO D,SUN G Z,et al.PTPRM methylation induced by FN1 promotes the development of glioblastoma by activating STAT3 signalling[J].Pharmaceutical Biology,2021,59(1):904-911.
[25] MARZEDA A M,MIDWOOD K S.Internal affairs:tenascin-C as a clinically relevant,endogenous driver of innate immunity[J].The Journal of Histochemistry and Cytochemistry,2018,66(4):289-304.  
[26] WIEMANN S,REINHARD J,REINEHR S,et al.Loss of the extracellular matrix molecule tenascin-C leads to absence of reactive gliosis and promotes anti-inflammatory cytokine expression in an autoimmune glaucoma mouse model[J].Frontiers in Immunology,2020,11:566279.
[27] POZZI A,YURCHENCO P D,IOZZO R V.The nature and biology of basement membranes[J].Matrix Biology,2017,57/58:1-11.
[28] LI Y C,LI J D,WANG X Y,et al.Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection[J].Virulence,2021,12(1):2352-2365.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2