动物营养学报 >

2022 , Vol. 34 >Issue 7: 4726 - 4736

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.07.059

研究论文 RESEARCH PAPER

向日葵副产物微贮复合发酵条件的响应曲面优化分析

  • 李肖 ,
  • 陈永成 ,
  • 许平珠 ,
  • 张凡凡 ,
  • 马春晖
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  • 1. 石河子大学动物科技学院, 石河子 832000;
    2. 新疆蓿之花草源生态有限公司, 哈巴河 836700
李肖(1998-),男,新疆伊犁人,硕士研究生,研究方向为饲草加工与生产。E-mail:744364871@qq.com

收稿日期: 2021-12-23

  网络出版日期: 2022-07-14

基金资助

农业农村部国家现代农业产业技术体系项目（CARS）；新疆维吾尔自治区优质饲草产业技术体系项目（SCTX-2021-03）

收起

Response Surface Optimization Analysis of Compound Fermentation Conditions of Sunflower By-Products Micro-Storage

  • LI Xiao ,
  • CHEN Yongcheng ,
  • XU Pingzhu ,
  • ZHANG Fanfan ,
  • MA Chunhui
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  • 1. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China;
    2. Xinjiang Xu zhi Hua Cao yuan ecology Co., Ltd., Habahe 836700, China

Received date: 2021-12-23

  Online published: 2022-07-14

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摘要

本试验旨在研究向日葵副产物的最优发酵条件，为该资源的合理利用提供基础。试验采用响应曲面优化法，在向日葵副产物青贮中添加乳酸菌、糖蜜、尿素和纤维素酶4种复合发酵剂，其中乳酸菌的添加量分别为1×105、5×105和1×106 CFU/g，尿素的添加量分别为0.1%、0.3%和0.5%，糖蜜的添加量分别为2%、3%和4%，纤维素酶的添加量分别为0.05%、0.10%和0.15%，选择中性洗涤纤维（NDF）和乳酸（LA）含量为优化目标，进行复合发酵条件30 d的厌氧发酵（真空袋法）。结果表明：NDF和LA含量的二次多项式模型均极显著（P<0.01），决定系数（R2）分别为0.88和0.82。其中，糖蜜和纤维素酶添加量对NDF含量的曲面效应影响极显著（P<0.01），尿素和纤维素酶添加量的交互作用对NDF含量的曲面效应影响显著（P<0.05）。乳酸菌添加量对LA含量的曲面效应影响极显著（P<0.01），糖蜜添加量对LA含量的曲面效应影响显著（P<0.05），乳酸菌和糖蜜添加量的交互作用对LA含量的曲面效应影响显著（P<0.05），糖蜜添加量分别与尿素、纤维素酶添加量的交互作用对LA含量的曲面效应影响显著（P<0.05）。最终优化结果为乳酸菌添加量9.3×105 CFU/g，糖蜜添加量3.57%，尿素添加量0.3%，纤维素酶的添加量0.14%。在此条件下厌氧发酵30 d，理论上可使NDF含量降低34.06%，LA含量提高134.74%。

关键词： 微贮; 向日葵; 发酵; 响应曲面分析

本文引用格式

李肖 , 陈永成 , 许平珠 , 张凡凡 , 马春晖 . 向日葵副产物微贮复合发酵条件的响应曲面优化分析[J]. 动物营养学报, 2022 , 34(7) : 4726 -4736 . DOI: 10.3969/j.issn.1006-267x.2022.07.059

Abstract

This experiment was conducted to study the optimal fermentation conditions of sunflower by-products, and to provide a basis for the rational utilization of this resource. Addition with lactic acid bacteria, molasses, urea and cellulase in the sunflower by-products silage through the response surface optimization method, the addition of lactic acid bacteria was 1×105, 5×105 and 1×106 CFU/g, respectively, the addition of urea was 0.1%,0.3% and 0.5%, respectively, the addition of molasses was 2%, 3% and 4%, respectively, and the addition of cellulase was 0.05%, 0.10% and 0.15%, respectively. The contents of neutral detergent fiber (NDF) and lactic acid (LA) were selected as the optimization objectives for anaerobic fermentation under compound fermentation conditions for 30 days (vacuum bag method). The results showed that the quadratic polynomial models of NDF and LA content were extremely significant (P<0.01), the coefficient of determination (R2) were 0.88 and 0.82, respectively. The additions of molasses and cellulase on the curved-surface effect of NDF content was significant (P<0.01), and the interaction of additions of urea and cellulase on the curved-surface effect of NDF content was significant (P<0.05). The addition of lactic acid bacteria on the curved-surface effect of LA content was significant (P<0.01), the addition of molasses on the curved-surface effect of LA content was significant (P<0.05), the interaction between the additions of lactic acid bacteria and molasses on the curved-surface effect of LA content was significant (P<0.05), and the interaction between the additions of molasses and urea and cellulase on the curved-surface effect of LA content was significant (P<0.05). The final optimized results shows that the addition of lactic acid bacteria is 9.3×105 CFU/g, the addition of molasses is 3.57%, the addition of urea is 0.3%, and the addition of cellulase is 0.14%. Under the condition of anaerobic fermentation for 30 days, the NDF content can be decreased by 34.06% and LA content can be increased by 134.74%.

Key words： micro storage; sunflower; fermentation; response surface analysis

参考文献

[1] DE TONISSI E BUSCHINELLI DE GOES R H,MIYAGI E S,DE OLIVEIRA E R,et al.Chemical changes in sunflower silage associated with different additives[J].Acta Scientiarum.Animal Sciences,2012,35(1):29-35.
[2] 新疆维吾尔自治区统计局,国家统计局新疆调查总队.新疆统计年鉴-2020[M].北京:中国统计出版社,2021. Statistics Bureau of Xinjiang Uygur Autonomous Region,Xinjiang Survey Corps of National Bureau of Statistics.Xinjiang statistical yearbook-2020[M].Beijing:China Statistics Press,2021.(in Chinese)
[3] 张佳,王园,安晓萍,等.向日葵副产物的营养特性及在反刍动物中的应用[J].中国畜牧兽医,2021,48(3):916-924. ZHANG J,WANG Y,AN X P,et al.Nutritional characteristics of sunflower by-products and its application in ruminants[J].China Animal Husbandry & Veterinary Medicine,2021,48(3):916-924.(in Chinese)
[4] GANDRA J R,OLIVEIRA E R,DE SENA GANDRA E R,et al.Inoculation of Lactobacillus buchneri alone or with Bacillus subtilis and total losses,aerobic stability,and microbiological quality of sunflower silage[J].Journal of Applied Animal Research,2017,45(1):609-614.  
[5] 田亚红,王巍杰,王之爽.向日葵花盘、秸秆发酵生产生物蛋白饲料工艺的研究[J].饲料工业,2013,34(11):42-45. TIAN Y H,WANG W J,WANG Z S.Study on fermentation technology using sunflower head and sunflower stalk to produce bioprotein feed[J].Feed Industry,2013,34(11):42-45.(in Chinese)
[6] POSTEMSKY P D,BIDEGAIN M A,LLUBERAS G,et al.Biorefining via solid-state fermentation of rice and sunflower by-products employing novel monosporic strains from Pleurotus sapidus[J].Bioresource Technology,2019,289:121692.
[7] MAFAKHER E,MESKARBASHEE M,HASSIBI P,et al.Study of chemical composition and quality characteristics of corn,sunflower and corn-sunflower mixture silages[J].Asian Journal of Animal and Veterinary Advances,2010,5(2):175-179.  
[8] 于杰,郑琛,李发弟,等.向日葵秸秆与全株玉米混合青贮饲料品质评定[J].草业学报,2013,22(5):198-204. YU J,ZHENG C,LI F D,et al.An evaluation of mixed-ensiling sunflower straw and whole corn on silage quality[J].Acta Prataculturae Sinica,2013,22(5):198-204.(in Chinese)
[9] DEMIREL M,BOLAT D,CELIK S,et al.Determination of fermentation and digestibility characteristics of corn,sunflower and combination of corn and sunflower silages[J].Journal of Animal and Veterinary Advances,2008,7(6):707-711.
[10] 赵士萍,周敏,蒋林树.青贮饲料添加剂的研究进展[J].中国农学通报,2016,32(20):6-10. ZHAO S P,ZHOU M,JIANG L S.Research progress of silage additive[J].Chinese Agricultural Science Bulletin,2016,32(20):6-10.(in Chinese)
[11] 黄媛,代胜,梁龙飞,等.不同添加剂对构树青贮饲料发酵品质及微生物多样性的影响[J].动物营养学报,2021,33(3):1607-1617. HUANG Y,DAI S,LIANG L F,et al.Effects of different additives on fermentation quality and microbial diversity of paper mulberry silage[J].Chinese Journal of Animal Nutrition,2021,33(3):1607-1617.(in Chinese)
[12] 叶杭,张文昌,陈国富,等.绿汁发酵液和纤维素酶对香蕉茎十小麦麸混合青贮品质的影响[J].中国畜牧兽医,2014,41(10):85-90. YE H,ZHANG W C,CHEN G F,et al.Effects of fermented green juice and cellulase on quality of banana stem and wheat bran mixed silage[J].China Animal Husbandry & Veterinary Medicine,2014,41(10):85-90.(in Chinese)
[13] 王建福,雷赵民,万学瑞,等.5株乳酸菌复合物与CaCO3、酶及尿素不同组合对全株玉米青贮品质影响[J].草业学报,2018,27(3):90-97. WANG J F,LEI Z M,WAN X R,et al.Effects on the quality of corn silage of 5 strains of lactic acid bacteria with different combinations of added CaCO3,enzyme and urea[J].Acta Prataculturae Sinica,2018,27(3):90-97.(in Chinese)
[14] 谢展,穆麟,张志飞,等.乳酸菌或有机酸盐与尿素复配添加对紫花苜蓿混合青贮的影响[J].草业学报,2021,30(5):165-173. XIE Z,MU L,ZHANG Z F,et al.Effects on fermentation in alfalfa mixed silage of added lactic acid bacteria or organic acid salt combined with urea[J].Acta Prataculturae Sinica,2021,30(5):165-173.(in Chinese)
[15] 刘婷婷,张振威,岳春旺,等.添加糖蜜和青贮添加剂对"张杂谷"全株青贮发酵品质的影响[J].中国畜牧兽医,2017,44(5):1382-1387. LIU T T,ZHANG Z W,YUE C W,et al.The effects of added molasses and additives on fermentation quality of "Zhang hybrid millet" whole plant silage[J].China Animal Husbandry & Veterinary Medicine,2017,44(5):1382-1387.(in Chinese)
[16] KONCA Y,BEYZI S B,KALIBER M,et al.Chemical and nutritional changes in sunflower silage associated with molasses,lactic acid bacteria and enzyme supplementation[J].Harran Journal of Agricultural and Food Science,2015,19(4):223-231.
[17] 王万中.试验的设计与分析[M].北京:高等教育出版社,2004. WANG W Z.The design of the test and analysis[M].Beijing:Higher Education Press,2004.(in Chinese)
[18] MOHAMMADZADEH H,KHORVASH M,GHORBANI G R.Ensiling corn silage with different levels of a multi-species lactic acid bacteria inoculant[J].Animal Production Science,2013,54(2):165-171.
[19] 唐振华,杨承剑,李孟伟,等.植物乳杆菌、布氏乳杆菌对甘蔗尾青贮品质及有氧稳定性的影响[J].中国畜牧兽医,2018,45(7):1824-1832. TANG Z H,YANG C J,LI M W,et al.Effect of applying Lactobacillus plantarum and Lactobacillus buchneri on quality and aerobic stability of sugarcane tops silage[J].China Animal Husbandry & Veterinary Medicine,2018,45(7):1824-1832.(in Chinese)
[20] 中华人民共和国国家质量监督检验检疫总局.实验动物配合饲料常规营养成分的测定:GB/T 14924.9-2001[S].北京:中国标准出版社,2002. General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China.Laboratory animals-formula feeds-determination of routine nutrients:GB/T 14924.9-2001[S].Beijing:Standards Press of China,2002.(in Chinese)
[21] 柳俊超,姜俊芳,宋雪梅,等.青贮饲料中主要有机酸的高效液相色谱分离分析[J].畜牧与兽医,2015,47(10):47-52. LIU J C,JIANG J F,SONG X M,et al.Separation and analysis of main organic acids in silage by high performance liquid chromatography[J].Animal Husbandry & Veterinary Medicine,2015,47(10):47-52.(in Chinese)
[22] 叶明,张爱忠,姜宁,等.NDF/NFC对反刍动物瘤胃微生物及发酵参数的调控作用[J].饲料研究,2015(22):4-9. YE M,ZHANG A Z,JIANG N,et al.Regulation of NDF/NFC on rumen microorganisms and fermentation parameters of ruminants[J].Feed Research,2015(22):4-9.(in Chinese)
[23] ZHANG F F,WANG X Z,LU W H,et al.Improved quality of corn silage when combining cellulose-decomposing bacteria and Lactobacillus buchneri during silage fermentation[J].BioMed Research International,2019,2019:4361358.
[24] WANG C,HAN H Y,SUN L,et al.Bacterial succession pattern during the fermentation process in whole-plant corn silage processed in different geographical areas of northern China[J].Processes,2021,9(5):900.
[25] WEBSTER J.The biochemistry of silage (second edition)[J].Experimental Agriculture,1992,28(1):125.
[26] ARBABI S,GHOORCH T.The effect of different levels of molasses as silage additives on fermentation quality of foxtail millet (Setaria italica) silage[J].Asian Journal of Animal Sciences,2008,2(2):43-50.  
[27] 张适,吴琼,尤欢,等.添加不同酶制剂对全株玉米青贮发酵品质的影响[J].中国畜牧兽医,2019,46(4):1045-1052. ZHANG S,WU Q,YOU H,et al.Effects of different enzyme preparations on fermentation quality of whole corn silage[J].China Animal Husbandry & Veterinary Medicine,2019,46(4):1045-1052.(in Chinese)
[28] ZHAO C,WANG L H,MA G M,et al.Cellulase interacts with lactic acid bacteria to affect fermentation quality,microbial community,and ruminal degradability in mixed silage of soybean residue and corn stover[J].Animals,2021,11(2):334.
[29] 李秀丽.氢氧化钠/尿素预处理强化木质纤维素酶解的研究[D].硕士学位论文.广州:华南理工大学,2015. LI X L.Study on enhancing hydrolysis of lignocellulosic biomass by NaOH/urea pretreatment[D].Master's Thesis.Guangzhou:South China University of Technology,2015.(in Chinese)
[30] 荣辉,余成群,李志华,等.添加糖蜜和尿素对象草青贮发酵品质的影响[J].草地学报,2012,20(5):940-946. RONG H,YU C Q,LI Z H,et al.Effects of adding molasses and urea on fermentation quality of napier grass silage[J].Acta Agrectir Sinica,2012,20(5):940-946.(in Chinese)
[31] 郭海明,夏天婵,朱雯,等.青贮添加剂对稻草青贮品质和有氧稳定性的影响[J].草业学报,2017,26(2):190-196. GUO H M,XIA T C,ZHU W,et al.Effect of additives on the quality and aerobic stability of rice straw silage[J].Acta Prataculturae Sinica,2017,26(2):190-196.(in Chinese)
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