动物营养学报 >

2022 , Vol. 34 >Issue 12: 7758 - 7769

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.12.027

研究论文 RESEARCH PAPER

笋头纤维对海兰褐蛋鸡育成期内脏器官指数、血浆激素含量和盲肠微生物区系的影响

  • 张世忠 ,
  • 陈秀琴 ,
  • 陈盛星 ,
  • 蓝豪杰 ,
  • 江斌 ,
  • 高炳辉 ,
  • 黄兴樑 ,
  • 王隆柏 ,
  • 刘景
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  • 1. 福建省农业科学院畜牧兽医研究所, 福州 350013;
    2. 中鲨动物保健品(厦门)有限公司, 厦门 361028;
    3. 福建明良食品有限公司, 南平 353100
张世忠(1976—),男,福建建瓯人,兽医师,硕士,研究方向为临床兽医、营养与饲养管理研究。E-mail:1161152782@qq.com

收稿日期: 2022-06-27

  网络出版日期: 2022-12-15

基金资助

福建省重大专项专题项目（2021NZ029023）；福建省省属公益类科研院所专项（2021R1026003）；福建省农业科学院科技示范基地项目

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Effects of Bamboo Shoot Head Fiber on Visceral Organ Indices, Plasma Hormone Contents and Cecal Microbiota of Hy-Line Brown Laying Hens during Growing Period

  • ZHANG Shizhong ,
  • CHEN Xiuqin ,
  • CHEN Shengxing ,
  • LAN Haojie ,
  • JIANG Bin ,
  • GAO Binghui ,
  • HUANG Xingliang ,
  • WANG Longbai ,
  • LIU Jing
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  • 1. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
    2. Joinsha Animal Health Products (Xiamen) Co., Ltd., Xiamen 361028, China;
    3. Fujian Mingliang Food Co., Ltd., Nanping 353100, China

Received date: 2022-06-27

  Online published: 2022-12-15

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摘要

本试验旨在研究饲粮添加笋头纤维对海兰褐蛋鸡育成期内脏器官指数、血浆激素含量和盲肠微生物区系的影响。试验选用384只8周龄健康海兰褐蛋鸡，随机分为4个组，每组4个重复，每个重复24只鸡。对照组饲喂基础饲粮，试验组在基础饲粮中分别添加10（试验Ⅰ组）、20（试验Ⅱ组）和30 g/kg （试验Ⅲ组）的笋头纤维。试验预试期7 d，正试期60 d。结果表明：1）与对照组相比，试验组蛋鸡肌胃指数显著提高（P<0.05），试验Ⅲ组肠道指数显著提高（P<0.05），试验Ⅱ组和试验Ⅲ组肠道长度和肠道校正长度显著提高（P<0.05）。2）与对照组相比，试验Ⅱ组和试验Ⅲ组蛋鸡血浆雌二醇含量显著降低（P<0.05），试验Ⅲ组血浆孕酮含量显著高于其他组（P<0.05）。3）与对照组相比，第31天，试验组蛋鸡盲肠菌群Shannon指数显著提高（P<0.05），试验组盲肠副拟杆菌属（Parabacteroides）、颤螺菌属（Oscillospira）和萨特氏菌属（Sutterella）相对丰度显著提高（P<0.05）；第61天，试验组蛋鸡盲肠菌群Chao1指数显著提高（P<0.05），试验组盲肠考拉杆菌属（Phascolarctobacterium）相对丰度显著降低（P<0.05）。差异物种分析表明，饲粮添加笋头纤维可显著降低蛋鸡盲肠甲烷杆菌科（Methanobacteriaceae）、肠球菌科（Enterococcaceae）和脱硫弧菌目（Desulfovibrionales）的相对丰度（P<0.05），显著提高盲肠巴恩斯氏菌科（Barnesiellaceae）、互养菌科（Synergistaceae）、克里斯滕森菌科（Christensenellaceae）、韦荣氏菌科（Veillonellaceae）和产碱菌科（Alcaligenaceae）的相对丰度（P<0.05）。综上所述，在育成期海兰褐蛋鸡饲粮中添加笋头纤维可改善蛋鸡胃肠道发育，改善血浆激素含量，提升盲肠菌群多样性和丰富度，并通过提高有益菌数量、抑制条件性致病菌繁殖，改善肠道健康，其中以30 g/kg添加水平效果较佳。

关键词： 蛋鸡; 育成期; 笋头纤维; 器官指数; 血浆激素; 盲肠微生物区系

本文引用格式

张世忠 , 陈秀琴 , 陈盛星 , 蓝豪杰 , 江斌 , 高炳辉 , 黄兴樑 , 王隆柏 , 刘景 . 笋头纤维对海兰褐蛋鸡育成期内脏器官指数、血浆激素含量和盲肠微生物区系的影响[J]. 动物营养学报, 2022 , 34(12) : 7758 -7769 . DOI: 10.3969/j.issn.1006-267x.2022.12.027

Abstract

This experiment was conducted to investigate the effects of dietary bamboo shoot head fiber on visceral organ indices, plasma hormone contents and cecal microbiota of Hy-Line brown laying hens during growing period. A total of 384 healthy Hy-Line brown laying hens of 8-week-old were randomly divided into 4 groups with 4 replicates per group and 24 hens per replicate. Hens in the control group were fed a basal diet, and those in the experimental groups were fed the basal diet supplemented with 10 (experimental group Ⅰ), 20 (experimental group Ⅱ) and 30 g/kg (experimental group Ⅲ) bamboo shoot head fiber, respectively. The pre-trial period lasted for 7 days and the experimental period lasted for 60 days. The results showed as follows:1) compared with the control group, the muscular stomach index of laying hens in all experimental groups was significantly increased (P<0.05), the intestine index in experimental group Ⅲ was significantly increased (P<0.05), and the intestine length and intestine correction length in experimental groups Ⅱ and Ⅲ were significantly increased (P<0.05). 2) Compared with the control group, the plasma estradiol content of laying hens in experimental groups Ⅱ and Ⅲ was significantly decreased (P<0.05), and the plasma progesterone content in experimental group Ⅲ was significantly higher than that in the other groups (P<0.05). 3) Compared with the control group, on day 31, the Shannon index of cecal microbiota of laying hens in all experimental groups was significantly increased (P<0.05), and the relative abundances of Parabacteroides, Oscillospira and Sutterella in cecum in all experimental groups were significantly increased (P<0.05); on day 61, the Chao1 index of cecal microbiota of laying hens in all experimental groups was significantly increased (P<0.05), and the Phascolarctobacterium relative abundance in cecum in all experimental groups was significantly decreased (P<0.05). The analysis of different species showed that dietary bamboo shoot head fiber could significantly reduce the relative abundances of Methanobacteriaceae, Enterococcaceae and Desulfovibrionales in cecum of laying hens (P<0.05), and significantly increase the relative abundances of Barnesiellaceae, Synergistaceae, Christensenellaceae, Veillonellaceae and Alcaligenace in cecum (P<0.05). In conclusion, dietary bamboo shoot head fiber for growing Hy-Line brown laying hens can improve the gastrointestinal development, plasma hormone content, cecal microbiota diversity and richness, and improve the intestine health by increasing the number of beneficial bacteria and inhibiting the propagation of conditional pathogenic bacteria, and the supplemental level of 30 g/kg has a better effect.

Key words： laying hens; growing period; bamboo shoot head fiber; organ index; plasma hormone; cecal microbiota

参考文献

[1] 韩淑云.日粮中添加可溶性纤维对蛋鸡采食行为、产蛋性能和蛋品质的影响[J].中国饲料, 2020(10):53-55. HAN S Y.The effect of dietary soluble fiber on the feeding behavior, laying performance and egg quality of laying hens[J].China Feed, 2020(10):53-55.(in Chinese)
[2] DAOU C, ZHANG H.Functional and physiological properties of total, soluble, and insoluble dietary fibres derived from defatted rice bran[J].Journal of Food Science and Technology, 2014, 51:3878-3885.
[3] 张茜, 杨莉, 刘毅.果皮果壳膳食纤维含量及性能测定[J].江西化工, 2014(4):116-118. ZHANG Q, YANG L, LIU Y.The content and determination of dietary fiber from peel husk[J].Jiangxi Chemical Industry, 2014(4):116-118.(in Chinese)
[4] 刘杰.雷竹笋复合饮料的研发与生产工艺研究[D].硕士学位论文.长沙:中南林科技大学, 2014. LIU J.Studies on the research & development and production process of the beverage of bamboo (Phyllostachys praecox C.D.Chu et C.S.Chao) shoot[D].Master's Thesis.Changsha:Central South University of Forestry and Technology, 2014.(in Chinese)
[5] SADEHI A, TOGHYANI M, GHEISARI A.Effect of various fiber types and choice feeding of performance, gut development, humoral immunity, and fiber preference in broiler chicks[J].Poultry Science, 2015, 94(11):2734-2743.  
[6] 丁冉冉, 徐彬, 孙全友, 等.不可溶性纤维对幼龄肉鸡生长性能以及肝脏脂质代谢的影响[J].动物营养学报, 2022, 34(5):2918-2927. DING R R, XU B, SUN Q Y, et al.Effects of insoluble fiber on growth performance and liver lipid metabolism of young broilers[J].Chinese Journal of Animal Nutrition, 2022, 34(5):2918-2927.(in Chinese)
[7] JIMÉNEZ-MORENO E, GONZÁLEZ-ALVARADO J M, GONZÁLEZ-SERRANO A, et al.Effect of dietary fiber and fat on performance and digestive traits of broilers from one to twenty-one days of age[J].Poultry Science, 2009, 88(12):2562-2574.  
[8] RÖHE I, METZGER F, VAHJEN W, et al.Effect of feeding different levels of lignocellulose on performance, nutrient digestibility, excreta dry matter, and intestinal microbiota in slow growing broilers[J].Poultry Science, 2020, 99(10):5018-5026.  
[9] 马晓聪, 熊兴江, 莫毅, 等.基于16S rDNA测序技术的自发性高血压大鼠肠道菌群结构变化及中药干预作用[J].中华中医药学刊, 2020, 38(8):71-74, 265-266. MA X C, XIONG X J, MO Y, et al.Study on changes of intestinal microflora in spontaneously hypertensive rats based on 16S rDNA sequencing and intervention of traditional Chinese medicine[J].Chinese Archives of Traditional Chinese Medicine, 2020, 38(8):71-74, 265-266.(in Chinese)
[10] 张世忠, 陈秀琴, 陈盛星, 等.单宁酸对白羽肉鸡生长性能、屠宰性能及肠道微生物菌群的影响[J].福建农业学报, 2021, 36(10):1153-1159. ZHANG S Z, CHEN X Q, CHEN S X, et al.Effects of tannic acid supplement on growth, slaughter performance, and gut microbiota of broilers[J].Fujian Journal of Agricultural Sciences, 2021, 36(10):1153-1159.(in Chinese)
[11] 叶保国, 王峰, 魏立民, 等.日粮粗纤维水平对35-70 d定安鹅生产性能及器官指数的影响[J].中国饲料, 2019(11):36-39. YE B G, WANG F, WEI L M, et al.Effects of dietary crude fibre levels on production performance and organ index of Ding'an geese aged from 35 to 70 days[J].China Feed, 2019(11):36-39.(in Chinese)
[12] 从光雷, 肖蕴祺, 张倩雲, 等.饲粮添加化香果单宁对肉鸡生长性能、屠宰性能、器官指数、肉品质、抗氧化功能和肠道发育的影响[J].动物营养学报, 2021, 33(5):2661-2671. CONG G L, XIAO Y Q, ZHANG Q Y, et al.Effects of plotytarya strohilacea Sieb.Et Zuce tannin on growth performance, slaughter performance, organ indexes, meat quality, antioxidant function and intestinal development of broilers[J].Chinese Journal of Animal Nutrition, 2021, 33(5):2661-2671.(in Chinese)
[13] 周建民, 邱凯, 张海军, 等.饲粮添加低聚木糖对蛋鸡蛋品质、血清抗氧化功能和脂质代谢的影响[J].动物营养学报, 2021, 33(7):3853-3862. ZHOU J M, QIU K, ZHANG H J, et al.Effects of dietary xylooligosaccharide on egg quality, serum antioxidant function and lipid metabolism of laying hens[J].Chinese Journal of Animal Nutrition, 2021, 33(7):3853-3862.(in Chinese)
[14] JIMÉNEZ-MORENO E, CHAMORRO S, FRIKHA M, et al.Effects of increasing levels of pea hulls in the diet on productive performance, development of the gastrointestinal tract, and nutrient retention of broilers from one to eighteen days of age[J].Animal Feed Science and Technology, 2011, 168(1/2):100-112.
[15] BAREKATAIN R, SWICK R A, TOGHYANI M, et al.Interactions of full-fat canola seed, oat hulls as an insoluble fiber source and pellet temperature for nutrient utilization and growth performance of broiler chickens[J].Poultry Science, 2017, 96(7):2233-2242.  
[16] 李刚, 雷福红, 欧阳吾乐, 等.苜蓿纤维对肉鸡生长性能、屠宰性能和器官指数的影响[J].饲料研究, 2021, 44(12):41-45. LI G, LEI F H, OU Y W L, et al.Effect of alfalfa dietary fiber on growth performance, slaughter performance and organ indices of broilers[J].Feed Research, 2021, 44(12):41-45.(in Chinese)
[17] 彭涵, 杨朝武, 陈家磊, 等.日粮纤维水平对大恒优质肉鸡胃肠道发育、免疫指标及血清生化指标的影响[J].中国家禽, 2019(24):34-39. PENG H, YANG C W, CHEN J L, et al.Effects of dietary fiber level on gastrointestinal tract development immune indices and serum biochemical indices of Daheng broiler[J].China Poultry, 2019(24):34-39.(in Chinese)
[18] 孙杰, 张星星, 杨亮, 等.蛋壳形成过程中部分生殖激素及其受体基因变化规律的研究[J].黑龙江畜牧兽医, 2014(3):87-89, 93. SUN J, ZHANG X X, YANG L, et al.Study on the variation regularity of parts of reproductive hormones and the receptor gene in the process of eggshell formation[J].Heilongjiang Animal Science and Veterinary Medicine, 2014(3):87-89, 93.(in Chinese)
[19] 宋扬, 李珏声, 吴晖云, 等.膳食纤维不同成分对大鼠体内雌激素水平的影响[J].营养学报, 1997(2):44-47. SONG Y, LI J S, WU H Y, et al.Effect of dietary fiber on estrogens levels in rats[J].Acta Nutrimenta Sinica, 1997(2):44-47.(in Chinese)
[20] 李刘冬, 李来好, 石红, 等.海藻膳食纤维对雌激素吸附作用的研究[J].中国海洋药物杂志, 2005, 24(3):1-4, 25. LI L D, LI L H, SHI H, et al.Adsorption effect of dietary fibers from seaweeds on estrogens[J].Chinese Journal of Marine Drugs, 2005, 24(3):1-4, 25.(in Chinese)
[21] 陈杨超, 张国祖, 吕明其, 等.6味中药超微粉对产蛋后期蛋鸡生产性能、蛋品质和生殖激素水平影响的研究[J].黑龙江畜牧兽医, 2018(8):163-165. CHEN Y C, ZHANG G Z, LYU M Q, et al.Effects of 6 kinds of Chinese medicine superfine powder on performance, egg quality and reproductive hormone levels of laying hens in late laying period[J].Heilongjiang Animal Science and Veterinary Medicine, 2018(8):163-165.(in Chinese)
[22] 张家才.孕酮对鸡蛋壳品质的影响和机制及外源调控研究[D].博士学位论文.武汉:华中农业大学, 2020:51-79. ZHANG J C.The effect and mechanism of progesterone on eggshell quality in laying hens and the study of exogenous regulation[D].Ph.D. Thesis.Wuhan:Huazhong Agricultural University, 2020:51-79.(in Chinese)
[23] 陈秀琴, 黄梅清, 郑敏, 等.病毒感染与肠道菌群关系的研究进展[J].中国兽医学报, 2019, 39(6):1239-1244. CHEN X Q, HUANG M Q, ZHENG M, et al.Research progress of the relationship between viral infection and gut microbiota[J].Chinese Journal of Veterinary Science, 2019, 39(6):1239-1244.(in Chinese)
[24] 张雪, 张珊, 钟光, 等.枯草芽孢杆菌对肉鸡生长性能、肠道组织形态和盲肠微生物组成的影响[J].动物营养学报, 2020, 32(11):5195-5208. ZHANG X, ZHANG S, ZHONG G, et al.Effects of Bacillus subtilis on growth performance, intestinal morphology and cecal microbial composition of broilers[J].Chinese Journal of Animal Nutrition, 2020, 32(11):5195-5208.(in Chinese)
[25] 陈祥宇, 耿浩川, 侯建库, 等.复合植物提取物对蛋鸡生产性能、肠道黏膜形态及盲肠菌群结构的影响[J].动物营养学报, 2021, 33(4):2033-2043. CHEN X Y, GENG H C, HOU J K, et al.Effects of compound plant extracts on performance, intestinal mucosal morphology and cecal microflora structure of laying hens[J].Chinese Journal of Animal Nutrition, 2021, 33(4):2033-2043.(in Chinese)
[26] SHANG H M, ZHAO J C, DONG X Q, et al.Inulin improves the egg production performance and affects the cecum microbiota of laying hens[J].International Journal of Biological Macromolecules, 2020, 155:1599-1609.
[27] EVANS N J, BROWN J M, MURRAY R D, et al.Characterization of novel bovine gastrointestinal tract Treponema isolates and comparison with bovine digital dermatitis treponemes[J].Applied and Environmental Microbiology, 2011, 77(1):138-147.  
[28] CHEN X Q, ZHENG M, LIN F Q, et al.Impacts of novel duck reovirus infection on the composition of intestinal microbiota of Muscovy ducklings[J].Microbial Pathogenesis, 2019, 137:103764.
[29] HORNE R, ST PIERRE J, ODEH S, et al.Microbe and host interaction in gastrointestinal homeostasis[J].Psychopharmacology, 2019, 236(5):1623-1640.  
[30] 白路平, 乔向宇, 海勒, 等.骆驼乳对慢性酒精性肝损伤小鼠肠道菌群的影响[J].中国食品学报, 2022, 22(1):78-87. BAI L P, QIAO X Y, HAI L, et al.Effect of camel milk on intestinal flora of mice with chronic alcoholic liver injury[J].Journal of Chinese Institute of Food Science and Technology, 2022, 22(1):78-87.(in Chinese)
[31] WU T R, LIN C S, CHANG C J, et al.Gut commensal Parabacteroides goldsteinii plays a predominant role in the anti-obesity effects of polysaccharides isolated from Hirsutella sinensis[J].Gut, 2019, 68(2):248-262.  
[32] LV W J, LIU C, YE C X, et al.Structural modulation of gut microbiota during alleviation of antibiotic-associated diarrhea with herbal formula[J].International Journal of Biological Macromolecules, 2017, 105(Pt.3):1622-1629.
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