研究论文 RESEARCH PAPER

植物乳杆菌对北京鸭脂质代谢和抗氧化功能的影响

  • 安柯颖 ,
  • 高文文 ,
  • 李蕾 ,
  • 夏兆飞
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  • 中国农业大学动物医学院, 北京 100193
安柯颖(1996—),女,河南焦作人,博士研究生,研究方向为家禽营养。E-mail:ankeying126@126.com

收稿日期: 2022-05-09

  网络出版日期: 2022-12-15

基金资助

现代农业产业技术体系北京市家禽创新团队(BAIC04-2021)

Effects of Lactobacillus plantarum on Lipid Metabolism and Antioxidant Function of Pekin Ducks

  • AN Keying ,
  • GAO Wenwen ,
  • LI Lei ,
  • XIA Zhaofei
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  • College of Veterinary Medicine, China Agricultural University, Beijing 100193, China

Received date: 2022-05-09

  Online published: 2022-12-15

摘要

本试验旨在探究植物乳杆菌对北京鸭脂质代谢和抗氧化功能的影响。选择180只1日龄北京鸭,随机分成3个组,每组6个重复,每个重复10只。对照组饲喂基础饲粮,试验组在基础饲粮中分别添加400(LP1组)和800 mg/kg植物乳杆菌(LP2组)。试验期42 d。结果表明:1)42日龄时,与对照组相比,LP1和LP2组的腹脂率分别降低了11.40%和5.03%(P>0.05),血清总胆固醇(TC)含量极显著降低(P<0.01)。2)21日龄时,与对照组相比,LP1和LP2组肝脏胆固醇7α-羟化酶(CYP7A1)的mRNA相对表达量均极显著升高(P<0.01)。42日龄时,与对照组相比,LP1和LP2组肝脏脂肪酸合成酶(FAS)和3-羟基3-甲基戊二酰辅酶A还原酶(HMGCR)的mRNA相对表达量均极显著降低(P<0.01),LP1组肝脏固醇调节元件结合蛋白-1c (SREBP-1c)的mRNA相对表达量极显著降低(P<0.01)。3)21日龄时,与对照组相比,LP1和LP2组血清过氧化氢酶(CAT)活性及肝脏超氧化物歧化酶(SOD)的mRNA相对表达量均极显著升高(P<0.01),回肠丙二醛(MDA)含量极显著降低(P<0.01);LP1组血清SOD活性显著升高(P<0.05),血清MDA含量显著降低(P<0.05)。42日龄时,与对照组相比,LP1和LP2组肝脏MDA含量极显著降低(P<0.01)。综上所述,饲粮中添加植物乳杆菌可通过减少合成、增强排泄机制来改善北京鸭的脂质代谢,同时提高其抗氧化功能。推荐北京鸭饲粮中植物乳杆菌添加剂量为400 mg/kg。

本文引用格式

安柯颖 , 高文文 , 李蕾 , 夏兆飞 . 植物乳杆菌对北京鸭脂质代谢和抗氧化功能的影响[J]. 动物营养学报, 2022 , 34(12) : 7790 -7800 . DOI: 10.3969/j.issn.1006-267x.2022.12.030

Abstract

This experiment aimed to investigate the effects of Lactobacillus plantarum on lipid metabolism and antioxidant function of Pekin ducks. One hundred and eighty 1-day-old Pekin ducks were randomly allocated to 3 groups with 6 replicates per group and 10 ducks per replicate. Ducks in the control group were fed a basal diet, and others in experimental groups were fed basal diets supplemented with 400 (LP1 group) and 800 mg/kg Lactobacillus plantarum (LP2 group), respectively. The experiment lasted for 42 days. The results showed as follows:1) at 42 days of age, compared with the control group, the percentage of abdominal fat of LP1 and LP2 groups was decreased by 11.40% and 5.03% (P>0.05), and the serum total cholesterol (TC) content was significantly decreased (P<0.01). 2) At 21 days of age, compared with the control group, the liver cholesterol 7α-hydroxylase (CYP7A1) mRNA relative expression level of LP1 and LP2 groups was significantly increased (P<0.01). At 42 days of age, compared with the control group, the mRNA relative expression levels of fatty acid synthase (FAS) and recombinant 3-hydroxy-3-methylglutaryl coenzyme a reductase (HMGCR) in liver of LP1 and LP2 groups were significantly decreased (P<0.01), and the liver sterol regulatory element-binding protein-1c (SREBP-1c) mRNA relative expression level of LP1 group was significantly decreased (P<0.01).3) At 21 days of age, compared with the control group, the serum catalase (CAT) activity and liver superoxide dismutase (SOD) mRNA relative expression level of LP1 and LP2 groups were significantly increased (P<0.01), and the ileal malondialdehyde (MDA) content was significantly decreased (P<0.01); the serum SOD activity of LP1 group was significantly increased (P<0.05), and the serum MDA content was significantly decreased (P<0.05). At 42 days of age, compared with the control group, the liver MDA content of LP1 and LP2 groups was significantly decreased (P<0.01). It is concluded that dietary Lactobacillus plantarum can improve the lipid metabolism of Peking duck by reducing synthesis and enhancing excretion mechanism, meanwhile improve the antioxidant function. The recommended supplemental dose of Lactobacillus plantarum in diet of Pekin ducks is 400 mg/kg.

参考文献

[1] SEDDIK H A, BENDALI F, GANCEL F, et al.Lactobacillus plantarum and its probiotic and food potentialities[J].Probiotics and Antimicrobial Proteins, 2017, 9(2):111-122.  
[2] ZHANG Z Y, LIU C, ZHU Y Z, et al.Safety assessment of Lactobacillus plantarum JDM1 based on the complete genome[J].International Journal of Food Microbiology, 2012, 153(1/2):166-170.
[3] REN D Y, LI C, QIN Y Q, et al.In vitro evaluation of the probiotic and functional potential of Lactobacillus strains isolated from fermented food and human intestine[J].Anaerobe, 2014, 30:1-10.
[4] LI C, NIE S P, DING Q, et al.Cholesterol-lowering effect of Lactobacillus plantarum NCU116 in a hyperlipidaemic rat model[J].Journal of Functional Foods, 2014, 8:340-347.
[5] CAO C X, WU R N, ZHU X Y, et al.Ameliorative effect of Lactobacillus plantarum WW-fermented soy extract on rat fatty liver via the PPAR signaling pathway[J].Journal of Functional Foods, 2019, 60:103439.
[6] GAO D W, GAO Z R, ZHU G H.Antioxidant effects of Lactobacillus plantarum via activation of transcription factor Nrf2[J].Food & Function, 2013, 4(6):982-989.  
[7] FU W J, STROMBERG A J, VIELE K, et al.Statistics and bioinformatics in nutritional sciences:analysis of complex data in the era of systems biology[J].The Journal of Nutritional Biochemistry, 2010, 21(7):561-572.  
[8] AN K Y, GAO W W, LI P, et al.Dietary Lactobacillus plantarum improves the growth performance and intestinal health of Pekin ducks[J].Poultry Science, 2022, 101(6):101844.
[9] SALAJ R, STOFILOVÁ J, SOLTESOVÁ A, et al.The effects of two Lactobacillus plantarum strains on rat lipid metabolism receiving a high fat diet[J].The Scientific World Journal, 2013, 2013:135142.
[10] BORGERAAS H, JOHNSON L K, SKATTEBU J, et al.Effects of probiotics on body weight, body mass index, fat mass and fat percentage in subjects with overweight or obesity:a systematic review and meta-analysis of randomized controlled trials[J].Obesity Reviews, 2018, 19(2):219-232.  
[11] SOHN M, NA G Y, CHU J, et al.Efficacy and safety of Lactobacillus plantarum K50 on lipids in Koreans with obesity:a randomized, double-blind controlled clinical trial[J].Frontiers in Endocrinology, 2022, 12:790046.
[12] GOLDSTEIN J L, BROWN M S.The LDL receptor[J].Arteriosclerosis, Thrombosis, and Vascular Biology, 2009, 29(4):431-438.  
[13] TRABELSI I, KTARI N, BEN SLIMA S, et al.Effects of supplementation with L. plantarum TN8 encapsulated in alginate-chitosan in broiler chickens[J].International Journal of Biological Macromolecules, 2016, 89:677-681.
[14] LI Y, LIU T J, ZHANG X, et al.Lactobacillus plantarum helps to suppress body weight gain, improve serum lipid profile and ameliorate low-grade inflammation in mice administered with glycerol monolaurate[J].Journal of Functional Foods, 2019, 53:54-61.
[15] WANG Y H, VISCARRA J, KIM S J, et al.Transcriptional regulation of hepatic lipogenesis[J].Nature Reviews Molecular Cell Biology, 2015, 16(11):678-689.  
[16] JONES S F, INFANTE J R.Molecular pathways:fatty acid synthase[J].Clinical Cancer Research, 2015, 21(24):5434-5438.  
[17] WANG H S, NI X Q, QING X D, et al.Live probiotic Lactobacillus johnsonii BS15 promotes growth performance and lowers fat deposition by improving lipid metabolism, intestinal development, and gut microflora in broilers[J].Frontiers in Microbiology, 2017, 8:1073.
[18] DAWOOD M A O, MAGOUZ F I, SALEM M F I, et al.Modulation of digestive enzyme activity, blood health, oxidative responses and growth-related gene expression in GIFT by heat-killed Lactobacillus plantarum (L-137)[J].Aquaculture, 2019, 505:127-136.
[19] WANG L F, LIU C H, CHEN M, et al.A novel Lactobacillus plantarum strain P-8 activates beneficial immune response of broiler chickens[J].International Immunopharmacology, 2015, 29(2):901-907.  
[20] KHANIAN M, KARIMI-TORSHIZI M A, ALLAMEH A.Alleviation of aflatoxin-related oxidative damage to liver and improvement of growth performance in broiler chickens consumed Lactobacillus plantarum 299v for entire growth period[J].Toxicon, 2019, 158:57-62.
[21] LUO J, YANG H Y, SONG B L.Mechanisms and regulation of cholesterol homeostasis[J].Nature Reviews Molecular Cell Biology, 2020, 21(4):225-245.  
[22] SONG Y F, XU C, SHAO S S, et al.Thyroid-stimulating hormone regulates hepatic bile acid homeostasis via SREBP-2/HNF-4α/CYP7A1 axis[J].Journal of Hepatology, 2015, 62(5):1171-1179.  
[23] QAYYUM F, LAURIDSEN B K, FRIKKE-SCHMIDT R, et al.Genetic variants in CYP7A1 and risk of myocardial infarction and symptomatic gallstone disease[J].European Heart Journal, 2018, 39(22):2106-2116.  
[24] CHEN Z, TIAN R F, SHE Z G, et al.Role of oxidative stress in the pathogenesis of nonalcoholic fatty liver disease[J].Free Radical Biology & Medicine, 2020, 152:116-141.
[25] WU D F, CEDERBAUM A I.Alcohol, oxidative stress, and free radical damage[J].Alcohol Research & Health, 2003, 27(4):277-284.
[26] CICHOŻ-LACH H, MICHALAK A.Oxidative stress as a crucial factor in liver diseases[J].World Journal of Gastroenterology, 2014, 20(25):8082-8091.  
[27] DEEB R S, HAJJAR D P.Repair mechanisms in oxidant-driven chronic inflammatory disease[J].American Journal of Pathology, 2016, 186(7):1736-1749.  
[28] SURAI P F, KOCHISH I I, FISININ V I, et al.Antioxidant defence systems and oxidative stress in poultry biology:an update[J].Antioxidants, 2019, 8(7):235.
[29] KOZIOROWKA-GILUN M, KOZIOROWSKI M, STRZEZEK J, et al.Seasonal changes in antioxidant defense systems in seminal plasma and fluids of the boar reproductive tract[J].Reproductive Biology, 2011, 11(1):37-47.  
[30] WU Y P, WANG B K, ZENG Z H, et al.Effects of probiotics Lactobacillus plantarum 16 and Paenibacillus polymyxa 10 on intestinal barrier function, antioxidative capacity, apoptosis, immune response, and biochemical parameters in broilers[J].Poultry Science, 2019, 98(10):5028-5039.  
[31] IZUDDIN W I, HUMAM A M, LOH T C, et al.Dietary postbiotic Lactobacillus plantarum improves serum and ruminal antioxidant activity and upregulates hepatic antioxidant enzymes and ruminal barrier function in post-weaning lambs[J].Antioxidants, 2020, 9(3):250.
[32] WANG J, ZHANG W, WANG S X, et al.Lactobacillus plantarum exhibits antioxidant and cytoprotective activities in porcine intestinal epithelial cells exposed to hydrogen peroxide[J].Oxidative Medicine and Cellular Longevity, 2021, 2021:8936907.
[33] TANG W, XING Z Q, LI C, et al.Molecular mechanisms and in vitro antioxidant effects of Lactobacillus plantarum MA2[J].Food Chemistry, 2017, 221:1642-1649.
[34] LIU Z Q, DONG L Y, JIA K Y, et al.Sulfonation of Lactobacillus plantarum WLPL04 exopolysaccharide amplifies its antioxidant activities in vitro and in a Caco-2 cell model[J].Journal of Dairy Science, 2019, 102(7):5922-5932.  
[35] WANG X, SHAO C G, LIU L, et al.Optimization, partial characterization and antioxidant activity of an exopolysaccharide from Lactobacillus plantarum KX041[J].International Journal of Biological Macromolecules, 2017, 103:1173-1184.
[36] XU Y M, CUI Y L, WANG X, et al.Purification, characterization and bioactivity of exopolysaccharides produced by Lactobacillus plantarum KX041[J].International Journal of Biological Macromolecules, 2019, 128:480-492.
[37] NOWAK A, PALIWODA A N, BŁASIAK J.Anti-proliferative, pro-apoptotic and anti-oxidative activity of Lactobacillus and Bifidobacterium strains:a review of mechanisms and therapeutic perspectives[J].Critical Reviews in Food Science and Nutrition, 2019, 59(21):3456-3467.  
[38] OLEKSY M, KLEWICKA E.Exopolysaccharides produced by Lactobacillus sp.:biosynthesis and applications[J].Critical Reviews in Food Science and Nutrition, 2018, 58(3):450-462.
[39] FENG T, WANG J.Oxidative stress tolerance and antioxidant capacity of lactic acid bacteria as probiotic:a systematic review[J].Gut Microbes, 2020, 12(1):1801944.
[40] SETH A, YAN F, POLK D B, et al.Probiotics ameliorate the hydrogen peroxide-induced epithelial barrier disruption by a PKC- and MAP kinase-dependent mechanism[J].American Journal of Physiology.Gastrointestinal and Liver Physiology, 2008, 294(4):G1060-G1069.
[41] LIU Y H, LIU Q, HESKETH J, et al.Protective effects of selenium-glutathione-enriched probiotics on CCl4-induced liver fibrosis[J].Journal of Nutritional Biochemistry, 2018, 58:138-149.
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