研究论文 RESEARCH PAPER

苏氨酸和异亮氨酸对皖西白鹅生长性能、盲肠组织结构、细胞因子和微生物区系的影响

  • 杨亚南 ,
  • 孙祁 ,
  • 徐廉竹 ,
  • 丁元翠 ,
  • 袁宇尘 ,
  • 胡倩倩 ,
  • 李升和 ,
  • 任曼
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  • 安徽科技学院动物科学学院, 动物营养调控与健康安徽省重点实验室, 凤阳 233100
杨亚南(1997—),男,安徽宿州人,硕士研究生,从事动物营养与健康相关研究。E-mail:1603555628@qq.com

收稿日期: 2022-05-31

  网络出版日期: 2022-12-15

基金资助

国家自然科学基金资助项目(31672502,31501968);安徽省科技重大专项计划项目(17030701004,201903a06020005);安徽省高校学科(专业)拔尖人才学术资助项目(gxbjZD2020080);省级大学生创新创业训练计划项目(S202010879113)

Effects of Threonine and Isoleucine on Growth Performance, Cecum Morphology, Cytokines and Microbiota of Wanxi White Geese

  • YANG Yanan ,
  • SUN Qi ,
  • XU Lianzhu ,
  • DING Yuancui ,
  • YUAN Yuchen ,
  • HU Qianqian ,
  • LI Shenghe ,
  • REN Man
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  • Anhui Key Laboratory of Animal Nutritional Regulation and Health, College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China

Received date: 2022-05-31

  Online published: 2022-12-15

摘要

本试验旨在研究苏氨酸和异亮氨酸对皖西白鹅生长性能、盲肠组织形态、细胞因子和微生物区系的影响。选取30日龄皖西白鹅180只,随机分为3组,每组3个重复,每个重复20只。对照组饲喂基础饲粮,苏氨酸组在基础饲粮中添加2.0 g/kg的苏氨酸,异亮氨酸组在基础饲粮中添加2.5 g/kg的异亮氨酸。试验期60 d。结果表明:1)苏氨酸组和异亮氨酸组61~90日龄料重比(F/G)极显著低于对照组(P<0.01)。2)60日龄时,异亮氨酸组盲肠绒毛高度和隐窝深度显著或极显著高于对照组(P<0.05或P<0.01)。90日龄时,苏氨酸组盲肠隐窝深度显著高于对照组(P>0.05),苏氨酸组和异亮氨酸组盲肠绒毛高度/隐窝深度显著低于对照组(P<0.05)。3)90日龄时,苏氨酸组和异亮氨酸组盲肠肿瘤坏死因子-α(TNF-α)含量显著低于对照组(P<0.05),苏氨酸组盲肠干扰素-γ(IFN-γ)和白细胞介素-4(IL-4)含量显著高于对照组(P<0.05)。4)90日龄时,异亮氨酸组盲肠软壁菌门(Tenericutes)相对丰度极显著高于对照组(P<0.01),苏氨酸组盲肠颤螺旋菌属(Oscillospira)、消化球菌属(Peptococcus)和S24_7科未分类菌属(unspecified_S24_7)相对丰度显著高于对照组(P<0.05)。由此可见,饲粮中添加2.0 g/kg苏氨酸或2.5 g/kg异亮氨酸均可一定程度提高皖西白鹅生长性能,异亮氨酸对60日龄盲肠组织结构的影响较大,苏氨酸对90日龄盲肠组织结构的影响较大。饲粮中添加2.5 g/kg异亮氨酸增加了皖西白鹅盲肠软壁菌门相对丰度,饲粮中添加2.0 g/kg苏氨酸增加了皖西白鹅盲肠颤螺旋菌属、消化球菌属和S24_7科未分类菌属相对丰度。

本文引用格式

杨亚南 , 孙祁 , 徐廉竹 , 丁元翠 , 袁宇尘 , 胡倩倩 , 李升和 , 任曼 . 苏氨酸和异亮氨酸对皖西白鹅生长性能、盲肠组织结构、细胞因子和微生物区系的影响[J]. 动物营养学报, 2022 , 34(12) : 7821 -7832 . DOI: 10.3969/j.issn.1006-267x.2022.12.033

Abstract

This experiment was conducted to study the effects of threonine and isoleucine on growth performance, cecum morphology, cytokines and microbiota of Wanxi white geese. A total of 180 thirty-day-old Wanxi white geese were randomly divided into 3 groups with 3 replicates per group and 20 geese per replicate. The control group was fed a basal diet, the threonine group was fed the basal diet supplemented with 2.0 g/kg threonine, and the isoleucine group was fed the basal diet supplemented with 2.5 g/kg isoleucine. The experiment lasted for 60 days. The results showed as follows:1) the feed to gain ratio (F/G) during 61 to 90 days of age of threonine group and isoleucine group was significantly lower than that of the control group (P<0.01). 2) At 60 days of age, the villous heigh and crypt depth in cecum of isoleucine group was significantly higher than that of the control group (P<0.05 or P<0.01). At 90 days of age, the crypt depth in cecum of threonine group was significantly higher than that of the control group (P<0.05), and the villous heigh/crypt depth in cecum of threonine group and isoleucine group was significantly lower than that of the control group (P<0.05). 3) At 90 days of age, the cecum tumor necrosis factor-α (TNF-α) content of threonine group and isoleucine group was significantly lower than that of the control group (P<0.05), the contents of interferon-γ (IFN-γ) and interleukin-4 (IL-4) in cecum of threonine group was significantly higher than that of the control group (P<0.05). 4) At 90 days of age, the cecum Tenericutes relative abundance of isoleucine group was significantly higher than that of the control group (P<0.01), and the relative abundances of Oscillospira, Peptococcus and unspecified_S24_7 in cecum of threonine group were significantly higher than those of the control group (P<0.05). In conclusion, dietary 2.0 g/kg threonine or 2.5 g/kg isoleucine can improve the growth performance of Wanxi white geese to a certain extent, isoleucine has a great effect on the cecum morphology at 60 days of age, and threonine has a great effect on the cecum morphology at 90 days of age. Dietary 2.5 g/kg isoleucine increases the cecum Tenericutes relative abundance of Wanxi white geese, and dietary 2.0 g/kg threonine increases the relative abundances of Oscillospira, Peptococcus and unspecified_S24_7 in cecum of Wanxi white geese.

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