动物营养学报 >

2022 , Vol. 34 >Issue 12: 8072 - 8085

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.12.054

研究论文 RESEARCH PAPER

宠物饲料中8种水溶性维生素同步分析方法研究

  • 严华 ,
  • 李兰 ,
  • 王继彤 ,
  • 贾铮 ,
  • 姚婷 ,
  • 刘佳 ,
  • 徐思远 ,
  • 田静 ,
  • 樊霞
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  • 1. 中国农业科学院农业质量标准与检测技术研究所, 北京 100081;
    2. 北京市兽药饲料监测中心, 北京 102200
严华(1978—),女,重庆人,副研究员,博士,主要从事饲料质量安全检测技术研究。E-mail:56431978@qq.com

收稿日期: 2022-05-31

  网络出版日期: 2022-12-15

基金资助

“十三五”国家重点研发计划项目课题（2019YFE0103800）

收起

Simultaneous Analysis of 8 Water Soluble Vitamins in Pet Feed

  • YAN Hua ,
  • LI Lan ,
  • WANG Jitong ,
  • JIA Zheng ,
  • YAO Ting ,
  • LIU Jia ,
  • XU Siyuan ,
  • TIAN Jing ,
  • FAN Xia
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  • 1. Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. Beijing Veterinary Drug and Feed Monitoring Center, Beijing 102200, China

Received date: 2022-05-31

  Online published: 2022-12-15

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摘要

本研究旨在建立宠物配合饲料中8种水溶性维生素（维生素B1、维生素B2、维生素B6、烟酸、烟酰胺、D-泛酸、D-生物素和叶酸）的同步分析方法。试验在宠物配合饲料样品中加入同位素内标，用1%盐酸溶液在50℃水浴40 min后，再用10%氨水溶液调节pH至6.0，沉淀后取上清液进行分析。以0.1%甲酸水溶液和0.1%甲酸乙腈溶液为流动相，SB-AQ C18色谱柱（100 mm×3.0 mm，1.8 μm）为分离柱，梯度洗脱，洗脱液经电喷雾离子源在正离子模式下进行电离，多反应监测模式采集数据，内标法定量。结果表明：8种水溶性维生素在5.0~2 000 ng/mL内具有良好的线性关系，相关系数（R2）大于0.995 0，犬粮和猫粮在3个添加水平下的加标回收率分别为80.0%~117.3%和81.0%~112.2%，相对标准偏差分别为1.04%~5.28%和1.06%~10.20%。该方法操作简便、过程快速、分析灵敏、结果准确，可实现宠物配合饲料中8种水溶性维生素的同时检测。

关键词： 宠物配合饲料; 水溶性维生素; 液相色谱-同位素稀释质谱; 内标法

本文引用格式

严华 , 李兰 , 王继彤 , 贾铮 , 姚婷 , 刘佳 , 徐思远 , 田静 , 樊霞 . 宠物饲料中8种水溶性维生素同步分析方法研究[J]. 动物营养学报, 2022 , 34(12) : 8072 -8085 . DOI: 10.3969/j.issn.1006-267x.2022.12.054

Abstract

The aim of this study was to establish a method for the simultaneous analysis of 8 water soluble vitamins (vitamin B1, vitamin B2, vitamin B6, niacin, nicotinamide, D-pantothenic acid, D-biotin and folic acid) in pet compound feed. In the experiment, the isotope internal standard was added to the pet compound feed samples, the samples were water bathed 40 min at 50℃ with 1% hydrochloric acid solution and the pH of the solutions were then adjusted to 6.0 by 10% ammonia solution. After precipitation, the supernatant was taken for analysis. The 0.1% formic acid aqueous solution and 0.1% formic acid acetonitrile solution were used as mobile phase, SB-AQ C18 chromatographic column (100 mm×3.0 mm, 1.8 μm) was used as the separation column, and gradient elution was performed. The eluate was ionized by electrospray ion source in positive ion mode, and the data were collected in multiple reaction monitoring mode, and quantified by internal standard method. The results showed that the eight water soluble vitamins had a good linear relationship in the range of 5.0 to 2 000 ng/mL, and the correlation coefficient (R2) was greater than 0.995 0. The recovery rates of dog food and cat food were 80.0% to 117.3% and 81.0% to 112.2%, respectively, and the relative standard deviations were 1.04% to 5.28% and 1.06% to 10.20%, respectively. The method is simple, rapid, sensitive and accurate, and can be used for the simultaneous detection of 8 water soluble vitamins in pet compound feed.

Key words： pet compound feed; water soluble vitamins; liquid chromatography-isotope dilution mass spectrometry; internal standard method

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