1 材料与方法
1.1 试验材料
1.2 试验设计
表1 基础饲粮组成及营养水平(风干基础)Table 1 Composition and nutrient levels of the basal diet (air-dry basis) % |
| 项目Items | 含量Content | ||
|---|---|---|---|
| 原料Ingredients | |||
| 玉米Corn | 56.80 | ||
| 豆粕Soybean meal | 23.30 | ||
| 豆油Soybean oil | 2.50 | ||
| 小麦麸Wheat bran | 4.40 | ||
| 菊花梗粉Chrysanthemum stalks powder | 4.70 | ||
| 石粉Limestone | 6.00 | ||
| 磷酸氢钙CaHPO4 | 1.50 | ||
| DL-蛋氨酸DL-methionine | 0.10 | ||
| 预混料Premix1) | 0.30 | ||
| 食盐NaCl | 0.40 | ||
| 合计Total | 100.00 | ||
| 营养水平Nutrient levels2) | |||
| 代谢能ME/(MJ/kg) | 11.35 | ||
| 粗纤维CF | 4.49 | ||
| 粗蛋白质CP | 16.21 | ||
| 粗灰分Ash | 9.49 | ||
| 粗脂肪EE | 6.92 | ||
| 钙Ca | 2.72 | ||
| 磷P | 0.69 | ||
| 蛋氨酸Met | 0.35 | ||
| 赖氨酸Lys | 0.89 | ||
| 苏氨酸Thr | 0.61 | ||
| 色氨酸Trp | 0.22 |
1)预混料为每千克饲粮提供 The premix provided the following per kg of the diet:VA 9 000 IU,VD3 2 000 IU,VE 40 mg,VK3 0.8 mg,VB1 2.0 mg,VB2 4.0 mg,烟酰胺 nicotinamide 30 mg,泛酸钙 calcium pantothenate 11 mg,VB6 4.0 mg,生物素 biotin 0.2 mg,叶酸 folic acid 0.5 mg,VB12 12 μg,Se 0.3 mg,Fe 80 mg,Mn 30 mg,Cu 4 mg,I 0.3 mg。 2)粗纤维、粗蛋白质、粗灰分、粗脂肪、钙、磷为实测值,其他为计算值。CF, CP, Ash, EE, Ca and P were measured values, while the others were calculated values. |
1.3 饲养管理
1.4 样品采集和指标测定
1.4.1 饲粮营养成分
1.4.2 生产性能
1.4.3 血清生殖激素、抗氧化和生化指标
1.5 统计分析
2 结果
2.1 不同锌源对产蛋期种鹅生产性能的影响
表2 不同锌源对产蛋期种鹅生产性能的影响Table 2 Effects of different zinc sources on performance of breeding geese during laying period |
| 项目 Items | 对照组 Control group | 蛋白锌组 Zn-Pro group | 蛋氨酸锌组 Zn-Met group | 甘氨酸锌组 Zn-Gly group | SEM | P值 P-value |
|---|---|---|---|---|---|---|
| 产蛋率LR/% | 34.87b | 34.07b | 40.29a | 39.64a | 0.917 | 0.016 |
| 平均日采食量ADFI/(g/d) | 183.64 | 182.82 | 178.44 | 181.58 | 0.910 | 0.228 |
| 平均蛋重AEW/g | 134.83 | 137.12 | 132.24 | 130.44 | 1.452 | 0.406 |
| 料蛋比F/E | 5.85a | 5.81a | 4.71b | 4.87ab | 0.180 | 0.033 |
| 产蛋总重EM/(kg/只) | 3.33 | 3.31 | 3.87 | 3.76 | 0.101 | 0.110 |
同行数据肩标不同小写字母表示差异显著(P<0.05),相同或无字母表示差异不显著(P>0.05)。下表同。 | |
In the same row, values with different small letter superscripts mean significant difference (P<0.05), while with the same or no letter superscripts mean no significant difference (P>0.05). The same as below. |
2.2 不同锌源对产蛋期种鹅血清生殖激素指标的影响
表3 不同锌源对产蛋期种鹅血清生殖激素指标的影响Table 3 Effects of different zinc sources on reproductive hormone indices of breeding geese during laying period |
| 项目 Items | 对照组 Control group | 蛋白锌组 Zn-Pro group | 蛋氨酸锌组 Zn-Met group | 甘氨酸锌组 Zn-Gly group | SEM | P值 P-value |
|---|---|---|---|---|---|---|
| 促卵泡素FSH/(mIU/L) | 1.36 | 1.37 | 1.52 | 1.39 | 0.045 | 0.539 |
| 促黄体激素LH/(mIU/L) | 2.46ab | 2.00b | 2.76a | 2.30ab | 0.096 | 0.030 |
| 催乳素PRL/(mIU/L) | 163.51 | 176.55 | 156.58 | 164.88 | 3.438 | 0.261 |
| 雌二醇E2/(pg/mL) | 72.65bc | 70.55c | 89.62a | 84.89ab | 2.814 | 0.020 |
2.3 不同锌源对产蛋期种鹅血清抗氧化指标的影响
表4 不同锌源对产蛋期种鹅血清抗氧化指标的影响Table 4 Effects of different zinc sources on serum antioxidant indices of breeding geese during laying period |
| 项目 Items | 对照组 Control group | 蛋白锌组 Zn-Pro group | 蛋氨酸锌组 Zn-Met group | 甘氨酸锌组 Zn-Gly group | SEM | P值 P-value |
|---|---|---|---|---|---|---|
| 总抗氧化能力T-AOC/(mmol/L) | 0.82 | 0.83 | 0.81 | 0.80 | 0.008 | 0.712 |
| 丙二醛MDA/(nmol/mL) | 2.72a | 2.01b | 1.68b | 1.80b | 0.125 | 0.010 |
| 超氧化物歧化酶SOD/(U/mL) | 18.64 | 19.20 | 19.86 | 19.39 | 0.250 | 0.432 |
| 谷胱甘肽过氧化物酶 GSH-Px/(U/mL) | 248.96 | 271.60 | 286.65 | 298.80 | 8.637 | 0.202 |
2.4 不同锌源对产蛋期种鹅血清生化指标的影响
表5 不同锌源对产蛋期种鹅血清生化指标的影响Table 5 Effects of different zinc sources on serum biochemical indices of breeding geese during laying period |
| 项目 Items | 对照组 Control group | 蛋白锌组 Zn-Pro group | 蛋氨酸锌组 Zn-Met group | 甘氨酸锌组 Zn-Gly group | SEM | P值 P-value |
|---|---|---|---|---|---|---|
| 总蛋白TP/(g/L) | 36.50 | 45.29 | 43.75 | 43.32 | 1.511 | 0.167 |
| 白蛋白ALB/(g/L) | 15.91 | 16.83 | 16.43 | 16.32 | 0.155 | 0.188 |
| 碱性磷酸酶AKP/(金氏单位/dL) | 2.36 | 2.47 | 2.44 | 2.47 | 0.021 | 0.209 |
| 甘油三酯TG/(mmol/L) | 1.48 | 1.25 | 0.90 | 1.20 | 0.103 | 0.273 |
| 谷草转氨酶AST/(U/L) | 29.92 | 29.03 | 29.53 | 32.25 | 0.635 | 0.314 |

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