1 材料与方法
1.1 试验材料
1.2 试验设计
1.3 基础饲粮和饲养管理
表1 基础饲粮组成及营养水平(干物质基础)Table 1 Composition and nutrient levels of the basal diet (DM basis) % |
| 原料 Ingredients | 含量 Content | 营养水平 Nutrient levels2) | 含量 Content | |
|---|---|---|---|---|
| 玉米秸秆Corn stalk | 67.00 | 有机物Organic matter | 89.21 | |
| 玉米Corn | 19.14 | 粗蛋白质Crude protein | 12.54 | |
| 棉籽粕Cottonseed meal | 7.18 | 纤维素Cellulose | 26.44 | |
| 菜籽饼Rapeseed cake | 2.39 | 半纤维素Hemi-cellulose | 26.39 | |
| 尿素Urea | 0.93 | 木质素Lignin | 3.69 | |
| 食盐NaCl | 0.50 | 钙Calcium | 0.79 | |
| 石粉Limestone | 1.20 | 磷Phosphorus | 0.34 | |
| 磷酸氢钙CaHPO4 | 0.80 | 总能Gross energy/(MJ/kg) | 17.12 | |
| 硫酸钠Na2SO4 | 0.50 | |||
| 预混料Premix1) | 0.36 | |||
| 合计Total | 100.00 |
1)预混料为每千克饲粮提供 The premix provided the following per kg of the diet:VA 1 350 IU,VD3 270 IU,VE 45 IU,Fe 16 mg,Cu 8 mg,Zn 5 mg,Mn 10 mg,I 8.5 mg,Co 0.10 mg,Se 0.20 mg。 | |
2)营养水平为实测值。Nutrient levels were measured values. |
1.4 样品的收集保存与处理
1.4.1 饲料和剩料样品的收集处理与保存
1.4.2 十二指肠和空肠食糜收集处理与保存
1.5 测定方法
1.5.1 概略养分的测定
1.5.2 十二指肠食糜样品的RNA和DNA含量测定
1.5.3 十二指肠和回肠食糜样品的氨基酸含量测定
1.6 计算方法
1.7 统计分析
2 结果
2.1 聚丙烯酰胺和磁化水对绵羊自由采食量的影响
表2 聚丙烯酰胺和磁化水对绵羊自由采食量的影响Table 2 Effects of polyacrylamide and magnetized water on voluntary feed intake of sheep (n=6) g/d |
| 项目 Items | 干物质 DM | 有机物 OM | 混合精料 Mixed concentrate | 玉米秸秆 Corn stalk |
|---|---|---|---|---|
| 对照组Control group | 759.4±92.9Bc | 681.2±84.5Bc | 223.1±23.4Bc | 536.3±57.3Bc |
| 聚丙烯酰胺组PAM group | 871.4±93.6Aa | 779.9±87.6Aa | 256.0±25.5Aa | 615.4±54.4Aa |
| 磁化水组MW group | 820.7±90.2Bb | 735.4±82.5Bb | 241.2±24.7Bb | 579.5±55.8Bb |
同列数据肩标不同小写字母表示差异显著(P<0.05),不同大写字母表示差异极显著(P<0.01),相同小写字母或无字母表示差异不显著(P>0.05)。 | |
In the same column, values with different small letter superscripts mean significant difference (P<0.05), and with different capital letter superscripts mean significant difference (P<0.01), while with the same small letter or no letter superscripts mean no significant difference (P>0.05). |
2.2 聚丙烯酰胺和磁化水对绵羊前胃营养物质消化吸收的影响
表3 聚丙烯酰胺和磁化水对绵羊前胃营养物质消化吸收的影响Table 3 Effects of polyacrylamide and magnetized water on nutrient digestion and absorption in fore-stomach of sheep (n=6) |
| 项目 Items | 对照组 Control group | 聚丙烯酰胺组 PAM group | 磁化水组 MW group |
|---|---|---|---|
| 营养物质消失率Nutrient disappearance rate/% | |||
| 干物质DM | 42.33±1.05 | 45.01±2.36 | 43.84±1.96 |
| 有机物OM | 46.30±1.65 | 48.46±2.04 | 48.19±1.68 |
| 粗蛋白质CP | 29.05±0.96 | 27.97±1.36 | 28.77±1.31 |
| 纤维素Cellulose | 56.99±2.66 | 59.46±2.74 | 58.21±2.12 |
| 半纤维素Hemicellulose | 63.00±2.96 | 65.33±3.58 | 64.64±3.62 |
| 营养物质消失量Nutrient disappearance amount/(g/d) | |||
| 干物质DM | 321.5±20.0C | 392.2±34.3A | 359.8±18.1B |
| 有机物OM | 286.5±14.1C | 345.2±34.9A | 318.8±10.8B |
| 粗蛋白质CP | 31.1±0.9Bc | 34.9±1.9Aa | 33.5±0.8Bb |
| 纤维素Cellulose | 138.1±14.4C | 169.0±10.4A | 155.1±4.5B |
| 半纤维素Hemicellulose | 59.8±6.8C | 73.3±11.5A | 66.9±8.3B |
同行数据肩标不同小写字母表示差异显著(P<0.05),不同大写字母表示差异极显著(P<0.01),相同小写字母或无字母表示差异不显著(P>0.05)。下表同。 | |
In the same row, values with different small letter superscripts mean significant difference (P<0.05), and with different capital letter superscripts mean significant difference (P<0.01), while with the same small letter or no letter superscripts mean no significant difference (P>0.05). The same as below. |
2.3 聚丙烯酰胺和磁化水对绵羊十二指肠营养物质消化吸收的影响
2.3.1 到达十二指肠常规营养物质含量
表4 聚丙烯酰胺和磁化水对绵羊到达十二指肠常规营养物质含量的影响Table 4 Effects of polyacrylamide and magnetized water on arriving duodenum routine nutrient contents of sheep (n=6) g/d |
| 项目 Items | 对照组 Control group | 聚丙烯酰胺组 PAM group | 磁化水组 MW group |
|---|---|---|---|
| 干物质DM | 437.9±17.6b | 479.2±14.4a | 460.9±22.4a |
| 有机物OM | 332.3±17.9c | 367.1±22.5a | 342.7±22.9b |
| 粗蛋白质CP | 76.04±3.76c | 89.75±4.12a | 82.89±4.21b |
| 微生物蛋白质MCP | 35.69±2.32C | 42.70±3.65A | 39.39±1.26B |
| 过瘤胃蛋白质Rumen bypass protein | 40.35±1.62Bc | 47.05±3.15Aa | 43.51±3.24Bb |
| 纤维素Cellulose | 104.3±10.5 | 115.2±10.8 | 111.4±7.6 |
| 半纤维素Hemicellulose | 35.1±2.3 | 38.9±3.7 | 36.6±4.0 |
| 钙Ca | 3.85±0.27Bb | 4.12±0.29Aa | 3.87±0.18Bb |
| 磷P | 3.09±0.13Bb | 3.40±0.18Aa | 3.18±0.15Bb |
2.3.2 到达十二指肠氨基酸含量
表5 聚丙烯酰胺和磁化水对绵羊到达十二指肠氨基酸含量的影响Table 5 Effects of polyacrylamide and magnetized water on arriving duodenum amino acid contents of sheep (n=6) |
| 项目 Items | 含量Content/(g/d) | 占总氨基酸比例Proportion of TAA/% | |||||
|---|---|---|---|---|---|---|---|
| 对照组 Control group | 聚丙烯酰胺组 PAM group | 磁化水组 MW group | 对照组 Control group | 聚丙烯酰胺组 PAM group | 磁化水组 MW group | ||
| 总氨基酸TAA | 44.10±6.15C | 50.46±5.75A | 48.40±6.53B | ||||
| 丙氨酸Ala | 1.71±0.18 | 1.87±0.23 | 1.82±0.10 | 3.88±0.50 | 3.71±0.75 | 3.76±0.24 | |
| 精氨酸Arg | 3.67±0.37 | 3.92±0.21 | 3.84±0.41 | 8.30±1.42a | 7.77±1.29b | 7.93±0.47b | |
| 天门冬氨酸Asp | 2.90±0.24 | 3.10±0.33 | 2.99±0.26 | 6.58±1.33a | 6.14±0.79b | 6.18±0.61b | |
| 半胱氨酸Cys | 0.61±0.03 | 0.67±0.03 | 0.62±0.04 | 1.39±0.18 | 1.33±0.07 | 1.28±0.14 | |
| 谷氨酸Glu | 5.22±0.45C | 6.64±0.32A | 6.55±0.33B | 11.81±2.14b | 13.16±2.75a | 13.54±3.40a | |
| 甘氨酸Gly | 4.22±0.24 | 4.71±0.21 | 4.44±0.23 | 9.56±1.43 | 9.33±0.91 | 9.16±2.28 | |
| 组氨酸His | 1.82±0.19b | 1.93±0.17a | 1.85±0.16b | 4.13±0.49 | 3.82±0.35 | 3.82±1.11 | |
| 异亮氨酸Ile | 2.84±0.24C | 3.21±0.35A | 3.03±0.15B | 6.45±0.97 | 6.36±0.72 | 6.27±1.19 | |
| 亮氨酸Leu | 4.04±0.39Bc | 5.02±0.34Aa | 4.89±0.44Ab | 9.15±1.68b | 9.95±2.35a | 10.10±0.53a | |
| 赖氨酸Lys | 3.77±0.28C | 4.23±0.49A | 4.06±0.32B | 8.52±0.47 | 8.38±0.45 | 8.39±0.43 | |
| 蛋氨酸Met | 0.55±0.03C | 0.68±0.04A | 0.62±0.04B | 1.25±0.07 | 1.35±0.10 | 1.28±0.09 | |
| 苯丙氨酸Phe | 2.54±0.16 | 2.66±0.17 | 2.55±0.23 | 5.76±0.32 | 5.27±0.29 | 5.26±0.48 | |
| 脯氨酸Pro | 1.95±0.11 | 2.12±0.13 | 1.97±0.10 | 4.42±0.61 | 4.20±0.35 | 4.07±0.46 | |
| 丝氨酸Ser | 1.78±0.19a | 1.97±0.21b | 1.94±0.26b | 4.04±0.43 | 3.90±0.44 | 4.01±0.25 | |
| 酪氨酸Tyr | 3.42±0.16 | 3.66±0.18 | 3.53±0.34 | 7.75±1.54 | 7.25±1.26 | 7.31±2.46 | |
| 苏氨酸Thr | 2.36±0.11C | 3.04±0.34A | 2.75±0.26B | 5.36±0.57 | 6.03±0.41 | 5.68±0.52 | |
| 缬氨酸Val | 0.72±0.04Bc | 1.04±0.06Aa | 0.96±0.05Ab | 1.63±0.13b | 2.06±0.07a | 1.98±0.18a | |
2.4 聚丙烯酰胺和磁化水对绵羊小肠营养物质消化吸收的影响
2.4.1 到达小肠常规营养物质含量
表6 聚丙烯酰胺和磁化水对绵羊到达小肠常规营养物质含量的影响Table 6 Effects of polyacrylamide and magnetized water on arriving small intestine routine nutrient contents of sheep (n=6) g/d |
| 项目 Items | 对照组 Control group | 聚丙烯酰胺组 PAM group | 磁化水组 MW group |
|---|---|---|---|
| 干物质DM | 85.81±7.27C | 115.11±8.34A | 102.68±8.81B |
| 有机物OM | 69.72±15.44C | 93.50±12.34A | 82.49±7.61B |
| 粗蛋白质CP | 47.41±13.55Bc | 56.62±4.36Aa | 52.24±14.30Bb |
| 纤维素Cellulose | 0.68±0.03C | 0.93±0.12A | 0.87±0.04B |
| 半纤维素Hemicellulose | 5.42±0.27C | 7.03±1.32A | 6.32±0.73B |
| 钙Ca | 1.26±0.11C | 1.64±0.15A | 1.53±0.14B |
| 磷P | 1.82±0.10Bc | 2.14±0.17Aa | 1.99±0.15Bb |
2.4.2 到达小肠氨基酸含量
表7 聚丙烯酰胺和磁化水对绵羊到达小肠氨基酸含量的影响Table 7 Effects of polyacrylamide and magnetized water on arriving small intestine amino acid contents of sheep (n=6) |
| 项目 Items | 含量Content/(g/d) | 占总氨基酸比例Proportion of TAA/% | |||||
|---|---|---|---|---|---|---|---|
| 对照组 Control group | 聚丙烯酰胺组 PAM group | 磁化水组 MW group | 对照组 Control group | 聚丙烯酰胺组 PAM group | 磁化水组 MW group | ||
| 总氨基酸TAA | 21.46±2.48Bb | 24.60±2.64Aa | 23.62±2.18Aa | ||||
| 丙氨酸Ala | 0.65±0.06b | 0.73±0.05a | 0.69±0.05 | 3.03±0.21 | 3.18±0.14 | 2.95±0.15 | |
| 精氨酸Arg | 1.26±0.07 | 1.35±0.09 | 1.30±0.12 | 5.87±1.23 | 6.03±1.32 | 5.55±1.21 | |
| 天门冬氨酸Asp | 1.46±0.12 | 1.56±0.12 | 1.58±0.18 | 6.80±1.65 | 6.76±1.39 | 6.75±1.86 | |
| 半胱氨酸Cys | 0.82±0.03 | 1.01±0.03 | 0.82±0.02 | 3.82±0.68 | 3.87±0.89 | 3.50±0.64 | |
| 谷氨酸Glu | 2.61±0.31Bb | 3.12±0.26Aa | 3.09±0.28Aa | 12.16±2.51 | 11.77±1.11 | 13.19±2.13 | |
| 甘氨酸Gly | 2.12±0.37Bb | 2.55±0.15Aa | 2.20±0.31Bb | 9.88±1.62 | 9.83±1.81 | 9.39±1.86 | |
| 组氨酸His | 0.75±0.04 | 0.76±0.03 | 0.78±0.05 | 3.49±0.39 | 3.58±0.28 | 3.33±0.38 | |
| 异亮氨酸Ile | 1.74±0.18b | 1.96±0.12a | 1.81±0.16 | 8.11±1.26 | 8.86±1.65 | 7.73±1.62 | |
| 亮氨酸Leu | 2.29±0.19Bb | 2.83±0.16Aa | 2.86±0.17Aa | 10.67±1.36 | 9.93±1.40 | 12.21±1.68 | |
| 赖氨酸Lys | 1.79±0.12Bc | 2.14±0.11Aa | 2.02±0.24Bb | 8.34±1.68 | 8.27±1.35 | 8.63±1.63 | |
| 蛋氨酸Met | 0.31±0.02C | 0.43±0.02A | 0.36±0.01B | 1.44±0.09 | 1.36±0.12 | 1.54±0.13 | |
| 苯丙氨酸Phe | 1.17±0.04 | 1.19±0.03 | 1.18±0.03 | 5.45±0.62 | 5.34±0.37 | 5.04±0.57 | |
| 脯氨酸Pro | 1.05±0.03 | 1.05±0.04 | 1.08±0.02 | 4.89±0.33 | 4.83±0.13 | 4.61±0.41 | |
| 丝氨酸Ser | 0.97±0.04b | 1.09±0.03a | 1.09±0.05a | 4.52±0.34 | 3.88±0.51 | 4.65±0.41 | |
| 酪氨酸Tyr | 1.35±0.08b | 1.47±0.09a | 1.41±0.12 | 6.29±0.52 | 6.32±0.30 | 6.02±0.31 | |
| 苏氨酸Thr | 1.05±0.07Bb | 1.36±0.07Aa | 1.27±0.06Aa | 4.89±0.24 | 5.28±0.51 | 5.42±0.40 | |
| 缬氨酸Val | 0.07±0.02Bc | 0.10±0.02Aa | 0.08±0.03Bb | 0.33±0.04 | 0.91±0.06 | 0.34±0.11 | |

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