1 材料与方法
1.1 发酵白酒糟制备
1.2 试验设计及饲粮配制
表1 试验饲粮组成及营养水平(风干基础)Table 1 Composition and nutrient levels of experimental diets (air-dry basis) |
| 项目 Items | 组别 Groups | |||||
|---|---|---|---|---|---|---|
| F0 | F5 | F10 | F15 | F20 | F25 | |
| 原料 Ingredients | ||||||
| 玉米 Corn | 49.54 | 47.08 | 43.47 | 42.70 | 44.21 | 44.59 |
| 小麦Wheat | 20.00 | 20.00 | 20.00 | 16.30 | 15.20 | 15.00 |
| 去皮豆粕 Peeled soybean meal | 16.50 | 14.10 | 12.10 | 10.90 | 10.00 | 9.10 |
| 全脂米糠 Full-fat rice bran | 10.00 | 10.00 | 10.00 | 10.00 | 5.00 | |
| 发酵白酒槽 Fermented white distiller’s grains | 5.00 | 10.00 | 15.00 | 20.00 | 25.00 | |
| 大豆油 Soybean oil | 0.20 | 0.80 | 1.50 | 2.10 | 2.90 | |
| 石粉 Limestone | 1.00 | 0.57 | 0.52 | 0.45 | 0.31 | 0.23 |
| L-赖氨酸 L-Lys | 0.46 | 0.55 | 0.61 | 0.65 | 0.68 | 0.68 |
| 预混料 Premix1) | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 |
| 合计 Total | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 |
| 营养水平 Nutrient levels2) | ||||||
| 消化能 DE/(MJ/kg) | 14.68 | 14.75 | 14.62 | 14.77 | 14.54 | 14.45 |
| 干物质 DM | 87.54 | 87.20 | 86.44 | 86.61 | 86.72 | 87.69 |
| 粗蛋白质 CP | 13.46 | 13.44 | 12.35 | 12.57 | 13.00 | 12.71 |
| 粗脂肪 EE | 3.73 | 3.13 | 3.62 | 4.70 | 2.44 | 2.60 |
| 粗纤维 CF | 2.04 | 2.21 | 3.12 | 3.20 | 3.29 | 3.62 |
| 粗灰分 Ash | 4.84 | 4.42 | 4.61 | 4.77 | 4.65 | 4.99 |
| 钙 Ca | 0.63 | 0.58 | 0.55 | 0.50 | 0.51 | 0.58 |
| 总磷 TP | 0.56 | 0.47 | 0.44 | 0.40 | 0.36 | 0.33 |
| 赖氨酸 Lys | 0.93 | 0.93 | 0.93 | 0.93 | 0.93 | 0.93 |
1)预混料为每千克饲粮提供 Premix provided the following per kilogram of diets:VA 6 000 IU,VD 2 000 IU,VE 30.00 mg,VK 31.50 mg,VB1 12.00 mg,VB4 25.00 mg,VB6 62.50 mg,VB12 0.20 mg,烟酸 nicotinic acid 20.00 mg,泛酸钙 calcium pantothenate 13.00 mg,叶酸 folic acid 0.35 mg,生物素 biotin 0.30 mg,胆碱 choline 300 mg,Fe 80 mg,Cu 15 mg,Zn 80 mg,Mn 20 mg,I 0.50 mg,Se 0.25 mg。 2)消化能和赖氨酸为计算值,其余均为实测值。DE and Lys were calculated values, while the others were measured values. |
1.3 样品采集与指标测定
1.3.1 样品采集
1.3.2 生长性能与经济效益
平均日采食量(kg/d)=总采食量/试验天数;
总增重(kg/头)=末重-初重;
平均日增重(kg/d)=总增重/试验天数;
料重比=平均日采食量/平均日增重;
增重成本(元/kg)=料重比×饲料价格;
生产效益(元/kg)=生猪价格-增重成本;
毛利润(元/头)=生产效益×总增重。
1.3.3 背最长肌肉品质
1.3.4 背最长肌中氨基酸组成
1.3.5 背最长肌中挥发性风味物质
OAVi=C/T;
ROAVi=(OAVi/OAVmax)×100。
1.3.6 背最长肌中脂肪酸组成
1.4 数据统计与分析
2 结果与分析
2.1 饲粮中添加不同水平发酵白酒糟对育肥猪生长性能的影响
表2 饲粮中添加不同水平发酵白酒糟对育肥猪生长性能的影响Table 2 Effects of dietary supplementation with different levels of fermented white distiller’s grains on growth performance of fattening pigs |
| 项目 Items | 组别Groups | 均值 标准误 SEM | P值P-value | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| F0 | F5 | F10 | F15 | F20 | F25 | 组别 Group | 线性 Linear | 二次 Quadratic | ||
| 平均日采食量ADFI/(kg/d) | 3.35 | 3.45 | 3.45 | 3.48 | 3.54 | 3.52 | 0.034 | 0.726 | 0.107 | 0.257 |
| 平均日增重ADG/(kg/d) | 1.09 | 1.13 | 1.16 | 1.10 | 1.11 | 1.01 | 0.017 | 0.190 | 0.128 | 0.033 |
| 料重比F/G | 3.07b | 3.08b | 2.98b | 3.17ab | 3.21ab | 3.52a | 0.050 | 0.016 | 0.004 | 0.001 |
同行数据肩标不同小写字母表示差异显著(P<0.05),相同或无字母表示差异不显著(P>0.05)。表5、表6、表8和表10同。 | |
In the same row, data with different lowercase letter superscripts indicated significant differences (P<0.05), while with the same or no letter superscripts indicated no significant difference (P>0.05). The same as Table 5, Table 6, Table 8 and Table 10. |
表3 发酵白酒糟添加水平与育肥猪生长性能指标的回归分析Table 3 Regression analysis between supplemental level of fermented white distiller’s and growth performance indexes of fattening pigs |
| 项目 Items | 回归方程 Regression equation | 决定系数 R2 | X极值 X-extremum | Y极值 Y-extremum |
|---|---|---|---|---|
| 平均日增重 ADG/(kg/d) | Y1=-0.013 393X2+0.078 821 1X+1.026 | 0.89 | 9.33 | 1.14 |
| 料重比 F/G | Y2=0.001 471X2-0.020 61X+3.092 1 | 0.79 | 7.01 | 3.02 |
表中X表示发酵白酒糟添加水平,Y1表示平均日增重,Y2表示料重比。 | |
In the table, X represented the supplemental level of fermented distiller’s grains, Y1 represented the ADG, and Y2 represented the F/G. |
2.2 饲粮中添加不同水平发酵白酒糟对育肥猪经济效益的影响
表4 饲粮中添加不同水平发酵白酒糟对育肥猪经济效益的影响Table 4 Effects of dietary supplementation with different levels of fermented white distiller’s grains on economic benefit of fattening pigs |
| 项目 Items | 组别Groups | |||||
|---|---|---|---|---|---|---|
| F0 | F5 | F10 | F15 | F20 | F25 | |
| 总增重 Total weight gain/(头/kg) | 65.55 | 67.50 | 69.45 | 66.00 | 66.45 | 60.60 |
| 饲料价格 Feed price/(元/kg) | 2.42 | 2.40 | 2.37 | 2.36 | 2.34 | 2.32 |
| 增重成本 Weight gain cost/(元/kg) | 7.42 | 7.38 | 7.06 | 7.47 | 7.52 | 8.16 |
| 生产效益 Production efficiency/(元/kg) | 6.02 | 6.06 | 6.38 | 5.97 | 5.92 | 5.28 |
| 毛利润 Gross profit/(元/头) | 394.39 | 409.05 | 443.32 | 394.06 | 393.49 | 319.93 |
2.3 饲粮中添加不同水平发酵白酒糟对育肥猪背最长肌肉品质的影响
表5 饲粮中添加不同水平发酵白酒糟对育肥猪背最长肌肉品质的影响Table 5 Effects of dietary supplementation with different levels of fermented white distiller’s grains on longissimus dorsi meat quality of fattening pigs |
| 项目 Items | 组别 Groups | 均值标准误 SEM | P值 P-value | |||||
|---|---|---|---|---|---|---|---|---|
| F0 | F5 | F10 | F15 | F20 | F25 | |||
| 亮度 L* | 42.25 | 42.77 | 43.28 | 44.33 | 44.73 | 44.72 | 0.403 | 0.327 |
| 红度 a* | 8.26 | 7.37 | 7.77 | 7.76 | 7.02 | 8.09 | 0.247 | 0.763 |
| 黄度 b* | 1.26 | 1.25 | 1.41 | 1.27 | 1.91 | 1.92 | 0.594 | 0.128 |
| pH | 6.57 | 6.43 | 6.46 | 6.51 | 6.42 | 6.48 | 0.044 | 0.899 |
| 蒸煮损失Cooking loss/% | 13.04 | 12.70 | 11.43 | 13.87 | 12.80 | 11.10 | 0.436 | 0.478 |
| 滴水损失 Drip loss/% | 2.04 | 1.74 | 1.99 | 1.55 | 2.59 | 2.46 | 0.137 | 0.169 |
| 背膘厚 Back fat thickness/mm | 25.74 | 27.16 | 27.65 | 22.79 | 27.20 | 27.14 | 0.711 | 0.367 |
2.4 饲粮中添加不同水平发酵白酒糟对育肥猪背最长肌中氨基酸组成的影响
表6 饲粮中添加不同水平发酵白酒糟对育肥猪背最长肌中氨基酸组成的影响Table 6 Effects of dietary supplementation with different levels of fermented white distiller’s grains on longissimus dorsis amino acid composition of fattening pigs |
| 项目 Items | 组别 Groups | 均值标准误 SEM | P值 P-value | |||||
|---|---|---|---|---|---|---|---|---|
| F0 | F5 | F10 | F15 | F20 | F25 | |||
| 天冬氨酸 Asp | 14.0 | 18.0 | 11.8 | 17.0 | 15.5 | 12.7 | 0.90 | 0.268 |
| 丝氨酸 Ser | 25.2 | 29.3 | 25.3 | 33.5 | 27.7 | 26.7 | 1.12 | 0.232 |
| 谷氨酸 Glu | 36.3 | 43.3 | 46.7 | 57.2 | 48.5 | 40.0 | 2.55 | 0.212 |
| 甘氨酸 Gly | 62.7 | 62.2 | 69.5 | 78.7 | 56.8 | 65.2 | 3.93 | 0.742 |
| 丙氨酸 Ala | 117.5 | 128.7 | 138.5 | 162.3 | 129.5 | 124.0 | 6.97 | 0.506 |
| 胱氨酸 Cys | 9.5b | 16.8ab | 12.8ab | 20.2a | 9.5b | 9.6b | 1.23 | 0.030 |
| 缬氨酸 Val | 20.3 | 26.3 | 22.7 | 28.8 | 23.0 | 23.2 | 0.96 | 0.111 |
| 蛋氨酸 Met | 11.5 | 15.0 | 11.7 | 17.5 | 14.5 | 10.5 | 0.93 | 0.202 |
| 异亮氨酸 Ile | 12.0 | 16.3 | 12.7 | 17.8 | 13.8 | 14.3 | 0.69 | 0.088 |
| 亮氨酸 Leu | 25.8 | 31.0 | 24.5 | 31.7 | 26.8 | 26.3 | 1.04 | 0.218 |
| 酪氨酸 Tyr | 41.2 | 39.5 | 36.5 | 44.0 | 32.0 | 38.8 | 1.55 | 0.330 |
| 苯丙氨酸 Phe | 53.8 | 55.7 | 34.8 | 48.2 | 37.3 | 51.6 | 3.50 | 0.389 |
| 赖氨酸 Lys | 68.5ab | 60.2ab | 45.7b | 71.5a | 50.2ab | 52.2ab | 2.73 | 0.017 |
| 组氨酸 His | 15.8 | 22.2 | 17.2 | 23.7 | 21.2 | 18.2 | 0.94 | 0.271 |
| 精氨酸 Arg | 14.5 | 19.0 | 53.8 | 24.0 | 77.3 | 55.0 | 11.18 | 0.081 |
| 脯氨酸 Pro | 14.5 | 19.0 | 53.8 | 24.0 | 77.3 | 55.0 | 11.18 | 0.611 |
| 必需氨基酸 EAA | 191.9 | 204.5 | 152.1 | 215.5 | 165.6 | 178.1 | 5.99 | 0.470 |
| 非必需氨基酸NEAA | 351.2 | 398.0 | 465.9 | 484.6 | 495.3 | 445.2 | 23.34 | 0.417 |
表7 各组育肥猪背最长肌中氨基酸的TAVTable 7 TAV of amino acids in longissimus dorsi of fattening pigs in different groups |
| 项目 Items | 组别 Groups | 阈值 Threshold | ||||||
|---|---|---|---|---|---|---|---|---|
| F0 | F5 | F10 | F15 | F20 | F25 | |||
鲜味氨基酸 Umami amino acids | 谷氨酸 | 22.28 | 23.96 | 25.19 | 27.23 | 24.46 | 21.39 | 0.3 |
| 天冬氨酸 | 2.58 | 2.99 | 1.91 | 2.43 | 2.35 | 2.04 | 1.0 | |
| 赖氨酸 | 25.23 | 19.98 | 14.79 | 20.43 | 15.19 | 16.75 | 0.5 | |
| 甘氨酸 | 8.88 | 7.94 | 8.65 | 8.65 | 6.61 | 8.05 | 1.3 | |
| 丙氨酸 | 36.06 | 35.60 | 37.35 | 38.64 | 32.66 | 33.16 | 0.6 | |
| 合计 | 95.02 | 90.47 | 87.89 | 97.37 | 81.27 | 81.38 | ||
甜味氨基酸 Sweetness amino acids | 组氨酸 | 14.55 | 18.42 | 13.92 | 16.93 | 16.04 | 14.60 | 0.2 |
| 脯氨酸 | 0.89 | 1.05 | 2.90 | 1.14 | 3.90 | 2.94 | 3.0 | |
| 丝氨酸 | 3.09 | 3.24 | 2.73 | 3.19 | 2.79 | 2.86 | 1.5 | |
| 合计 | 18.53 | 22.72 | 19.55 | 21.26 | 22.73 | 20.40 | ||
苦味氨基酸 Bitter taste amino acids | 蛋氨酸 | 7.06 | 8.30 | 6.31 | 8.33 | 7.31 | 5.62 | 0.3 |
| 精氨酸 | 5.34 | 6.31 | 17.41 | 6.86 | 23.39 | 17.65 | 0.5 | |
| 缬氨酸 | 9.34 | 10.91 | 9.18 | 10.28 | 8.70 | 9.31 | 0.4 | |
| 亮氨酸 | 2.50 | 2.71 | 2.09 | 2.38 | 2.13 | 2.22 | 1.9 | |
| 异亮氨酸 | 2.46 | 3.01 | 2.28 | 2.82 | 2.32 | 2.55 | 0.9 | |
| 苯丙氨酸 | 11.01 | 10.27 | 6.26 | 7.65 | 6.27 | 9.20 | 0.9 | |
| 合计 | 37.70 | 41.50 | 43.53 | 38.33 | 50.13 | 46.54 | ||
2.5 饲粮中添加不同水平发酵白酒糟对育肥猪背最长肌中挥发性风味物质的影响
图1 各组育肥猪背最长肌中挥发性风味物质的种类和相对含量Fig.1 Types and relative contents of volatile flavor substances in longissimus dorsi of fattening pigs in different groups |
表8 饲粮中添加不同水平发酵白酒糟对育肥猪背最长肌中挥发性风味物质相对含量的影响Table 8 Effects of dietary supplementation with different levels of fermented white distiller’s grains on volatile flavor substance relative contents in longissimus dorsis of fattening pigs |
| 项目 Items | 组别 Groups | 均值标准误 SEM | P值 P-value | ||||||
|---|---|---|---|---|---|---|---|---|---|
| F0 | F5 | F10 | F15 | F20 | F25 | ||||
醛类 Aldehydes | 苯甲醛 | 0.10b | 0.11b | 0.20a | 0.15ab | 0.16ab | 0.13ab | 0.009 | 0.016 |
| 壬醛 | 4.94 | 5.72 | 6.70 | 5.58 | 5.48 | 5.52 | 0.858 | 0.997 | |
| 反式-2-壬烯醛 | 0.07 | 0.08 | 0.09 | 0.10 | 0.09 | 0.10 | 0.022 | 0.180 | |
| 癸醛 | 0.95 | 1.12 | 1.45 | 1.32 | 1.66 | 1.18 | 0.133 | 0.729 | |
| 十二醛 | 0.24 | 0.17 | 0.18 | 0.31 | 0.17 | 0.18 | 0.023 | 0.423 | |
| 肉豆蔻醛 | 0.29 | 0.32 | 0.40 | 0.40 | 0.25 | 0.21 | 0.040 | 0.709 | |
烃类 Hydrocarbons | 2-溴十八烷 | 0.22 | 0.31 | 0.32 | 0.47 | 0.40 | 0.28 | 0.039 | 0.123 |
| 十四烷 | 0.49 | 0.33 | 0.46 | 0.35 | 0.71 | 0.54 | 0.068 | 0.657 | |
| 十六烷 | 0.21 | 0.23 | 0.22 | 0.19 | 0.25 | 0.20 | 0.014 | 0.886 | |
| 正十九烷 | 0.39 | 0.23 | 0.31 | 0.23 | 0.57 | 0.36 | 0.054 | 0.469 | |
| 1,2-环氧基十六烷 | 0.30 | 0.15 | 0.19 | 0.22 | 0.21 | 0.19 | 0.017 | 0.286 | |
醇类 Alcohols | 1-辛烯-3-醇 | 0.18 | 0.25 | 0.26 | 0.23 | 0.21 | 0.20 | 0.016 | 0.724 |
| 辛醇 | 0.85 | 0.91 | 1.56 | 1.48 | 1.45 | 0.89 | 0.134 | 0.396 | |
| 壬醇 | 0.27 | 0.28 | 0.32 | 0.31 | 0.32 | 0.42 | 0.040 | 0.913 | |
| 2,4-二叔丁基苯酚 | 0.19 | 0.21 | 0.22 | 0.19 | 0.22 | 0.16 | 0.019 | 0.936 | |
| 2-[(Z)-十八碳癸-9- 烯氧基]乙醇 | 0.18 | 0.13 | 0.24 | 0.23 | 0.24 | 0.47 | 0.057 | 0.639 | |
| 邻苯二甲酸二异丁酯 | 0.36 | 0.29 | 0.19 | 0.16 | 0.33 | 0.25 | 0.044 | 0.769 | |
| 邻苯二甲酸二丁酯 | 0.40 | 0.60 | 0.36 | 0.37 | 0.71 | 0.53 | 0.086 | 0.837 | |
酸类 Acids | N-羟基苯羧酸 | 1.66a | 0.75b | 0.88b | 0.46c | 0.79b | 0.47c | 0.105 | 0.004 |
| 花生四烯酸 | 0.27ab | 0.13b | 0.32ab | 0.49ab | 0.25ab | 0.70a | 0.055 | 0.034 | |
| 癸酸 | 0.50 | 0.57 | 0.47 | 0.44 | 0.38 | 0.44 | 0.050 | 0.935 | |
| N,N-二乙基氨基 甲二硫酸 | 0.83ab | 1.24ab | 1.01ab | 0.56b | 1.93a | 0.94ab | 0.129 | 0.036 | |
其他 Others | 2,6-二甲基苯甲醛、 硫代氨基甲脲 | 0.26 | 0.48 | 0.42 | 0.79 | 1.33 | 0.99 | 0.147 | 0.282 |
| 2-(7-七炔氧基) 四氢-2H-吡喃 | 1.27 | 2.27 | 2.44 | 1.51 | 0.54 | 0.99 | 0.379 | 0.710 | |
表9 各组育肥猪背最长肌中挥发性风味物质的ROAVTable 9 ROAV of volatile flavor substances in longissimus dorsi of fattening pigs in different groups |
| 项目 Items | 组别 Groups | 感官阈值 Sensory threshold/ (ng/g) | 气味描述 Description of smell | ||||||
|---|---|---|---|---|---|---|---|---|---|
| F0 | F5 | F10 | F15 | F20 | F25 | ||||
醛类 Aldehydes | 反式-2-辛烯醛 | 0.40 | 0.48 | 0.56 | 0.72 | 1.02 | 0.58 | 5.00 | 香蕉味 |
| 壬醛 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 1.10 | 油脂味、柑橘味 | |
| 反式-2-壬烯醛 | 19.52 | 20.89 | 25.07 | 28.97 | 25.07 | 26.93 | 0.08 | 强烈的黄瓜气味 | |
| 反式-2-癸烯醛 | 0.02 | 0.01 | 0.01 | 0.02 | 0.03 | 0.02 | 135.00 | 泥土味、蘑菇味 | |
| 肉豆蔻醛 | 0.06 | 0.06 | 0.08 | 0.08 | 0.05 | 0.04 | 110.00 | 花香味、蜡味 | |
醇类 Alcohols | 1-辛烯-3-醇 | 2.65 | 3.76 | 3.84 | 3.34 | 3.09 | 2.97 | 1.50 | 蘑菇味、薰衣草、 玫瑰香气 |
| 辛醇 | 0.15 | 0.16 | 0.28 | 0.26 | 0.26 | 0.16 | 125.80 | 脂肪味、水果味 | |
| 壬醇 | 0.12 | 0.12 | 0.14 | 0.14 | 0.14 | 0.19 | 50.00 | 玫瑰香气、柑橘味 | |
2.6 饲粮中添加不同水平发酵白酒糟对育肥猪背最长肌中脂肪酸组成的影响
表10 饲粮中添加不同水平发酵白酒糟对育肥猪背最长肌中脂肪酸组成的影响Table 10 Effects of dietary supplementation with different levels of fermented white distiller’s grains on fatty acid composition in longissimus dorsi of fattening pigsμg/g |
| 项目 Items | 组别 Groups | 均值表准误 SEM | P值 P-value | ||||||
|---|---|---|---|---|---|---|---|---|---|
| F0 | F5 | F10 | F15 | F20 | F25 | ||||
饱和脂肪酸 Saturated fatty acids | 癸酸 | 13.41 | 10.62 | 16.46 | 19.71 | 12.09 | 14.93 | 1.624 | 0.672 |
| 肉豆蔻酸 | 122.94 | 101.26 | 201.97 | 115.57 | 144.20 | 126.57 | 12.364 | 0.230 | |
| 棕榈酸 | 1 870.09b | 1 937.58b | 1 877.96b | 2 183.48ab | 2 951.34a | 2 548.04ab | 90.948 | 0.001 | |
| 十七碳酸 | 13.51 | 12.25 | 22.04 | 14.14 | 16.23 | 17.58 | 1.350 | 0.343 | |
| 硬脂酸 | 1 244.97 | 1 061.95 | 1 505.50 | 1 117.75 | 1 390.33 | 1 322.94 | 83.077 | 0.692 | |
| 花生酸 | 19.26 | 15.74 | 29.82 | 16.96 | 15.37 | 19.95 | 1.714 | 0.136 | |
| 二十三碳酸 | 16.69b | 33.07b | 120.52a | 136.19a | 117.07a | 132.95a | 9.279 | 0.001 | |
| 合计 | 3 300.87 | 3 172.46 | 3 774.27 | 3 603.79 | 4 646.62 | 4 182.96 | |||
单不饱和脂肪 Monounsaturated fatty acids | 棕榈油酸 | 290.93 | 228.03 | 376.26 | 254.67 | 300.12 | 291.89 | 19.379 | 0.359 |
| 顺-10-十七 碳一烯酸 | 13.46 | 10.98 | 19.09 | 12.73 | 14.84 | 15.32 | 1.050 | 0.336 | |
| 油酸 | 16.71 | 14.26 | 16.58 | 15.60 | 14.62 | 15.46 | 0.612 | 0.842 | |
| 顺-11-二十 碳一烯酸 | 54.45 | 57.03 | 99.20 | 66.46 | 71.31 | 41.19 | 5.755 | 0.066 | |
| 顺-15-二十四 碳一烯酸 | 9.45b | 37.78a | 8.93b | 21.26ab | 15.96ab | 2.30b | 3.080 | 0.007 | |
| 合计 | 384.99 | 348.08 | 520.06 | 370.72 | 416.85 | 366.16 | |||
多不饱和脂肪酸 Polyunsaturated fatty acids | 反式亚油酸 | 4.60 | 3.98 | 7.07 | 4.58 | 4.79 | 5.27 | 0.791 | 0.435 |
| 亚油酸 | 971.58ab | 1 081.72ab | 1 346.79a | 1 271.96ab | 933.06b | 1 139.66ab | 43.653 | 0.033 | |
| α-亚麻酸 | 25.40 | 36.04 | 51.21 | 47.62 | 35.23 | 36.94 | 3.562 | 0.340 | |
| 顺,顺-11-14- 二十碳二烯酸 | 40.83 | 38.68 | 51.88 | 40.18 | 25.49 | 40.63 | 2.472 | 0.072 | |
| 顺,顺,顺-8,11- 14-二十碳三烯酸 | 18.11 | 18.74 | 21.13 | 18.55 | 12.05 | 12.89 | 1.039 | 0.057 | |
| 花生四烯酸 | 129.50 | 116.60 | 93.40 | 46.90 | 48.51 | 59.67 | 10.943 | 0.098 | |
| 顺-5,8,11,14,17- 二十碳五烯酸 | 8.57 | 9.25 | 8.33 | 7.46 | 7.53 | 6.56 | 0.499 | 0.743 | |
| 顺-4,7,10,13,16, 19-二十二碳六烯酸 | 4.86ab | 4.83ab | 4.62ab | 5.84ab | 3.21b | 6.90a | 0.317 | 0.014 | |
| 合计 | 1 203.45 | 1 309.84 | 1 584.43 | 1 443.09 | 1 069.87 | 1 308.52 | |||

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