本试验旨在研究饲粮添加过瘤胃赖氨酸（rumen protected lysine，RPLys）和过瘤胃蛋氨酸（rumen protected methionine，RPMet）对泌乳水牛生产性能的影响。选择15头泌乳前期、健康状态良好的奶水牛，按品种、产奶量、产犊时间及胎次相近的原则随机分成5组，每组3头。采用5×5拉丁方试验设计，试验分5期（每期持续21 d），5个饲粮处理分别为对照1（基础饲粮1，精料粗蛋白质水平=16%）、对照2（基础饲粮2，精料粗蛋白质水平=20%)；处理1（基础饲粮1+RPLys 40 g/d）；处理2（基础饲粮1+RPMet 15 g/d）、处理3（基础饲粮1+RPLys 30 g/d+RPMet 6 g/d）。结果表明：1）与对照1比较，添加RPLys、RPMet对泌乳水牛产奶量的影响差异不显著(P>0.05)，但能在一定程度上提高泌乳水牛的产奶量，其中单独添加RPLys效果较好。与对照1和对照2相比，处理1的产奶量分别提高了10.0%和5.9%。2）添加RPLys、RPMet对泌乳水牛干物质采食量的影响差异不显著（P>0.05）。3）添加RPLys和RPMet能提高泌乳奶牛乳蛋白率、乳糖率和乳总固形物、乳非脂固形物含量，其中对乳蛋白率的影响差异显著（P<0.05）。与对照1相比，处理1、处理2和处理3的乳蛋白率分别提高了29.0%（P>0.05）、36.8%（P<0.05）和54.3%（P<0.05）；与对照2相比，处理1、处理2和处理3的乳蛋白率分别提高了3.9%（P>0.05）、10.2%（P>0.05）和24.4%（P>0.05）。综上所述，泌乳水牛饲粮中添加RPLys和RPMet能一定程度提高泌乳水牛的生产性能，改善乳品质。

This experiment was conducted to determine the effects of dietary supplementation of rumen-protected lysine (RPLys) and rumen-protected methionine (RPMet) on performance of lactating water buffalo. Fifteen healthy water buffaloes in early lactation were selected, and according to species, milk yield, calving time and similar parity, the experimental animals were randomly divided into 5 groups, and 3 lactating water buffaloes for each group, using 5×5 Latin square design with 5 periods (each period lasted for 21 days) and 5 groups, i.e. control 1 (basal diet 1, crude protein level of concentrate=16%), control 2 (basal diet 2, crude protein level of concentrate=20%), treatment 1 (basal diet 1+RPLys 40 g/d), treatment 2 (basal diet 1+RPMet 15 g/d), and treatment 3 (basal diet 1+RPLys 30 g/d+RPMet 6 g/d). The results showed as follows: 1) there was no significant difference in milk yield of lactating water buffalo among all treatments (P>0.05), but supplementation of RPLys or RPMet could increase the milk yield of lactating water buffalo to some extent and supplementation of RPLys had better effects than RPMet and RPMet+RPLys supplementation. Compared with control 1 and control 2, the supplementation of RPLys increased the milk yield by 10.0% and 5.9% (P>0.05). 2) There was no significant difference in dry matter intake of lactating water buffalo among all treatments (P>0.05). 3) The supplementation of RPLys and RPMet could improve milk protein rate, milk lactose rate, and milk total solid and milk non-fat solid contents of lactating water buffalo. Compared with control 1, the milk protein rate of the treatments with supplementation of RPLys, RPMet and RPLys+RPMet were increased by 29.0% (P>0.05), 36.8% (P＜0.05) and 54.3% (P＜0.05), respectively; and compared with control 2, the milk protein rate of the treatments with supplementation of RPLys, RPMet and RPLys+RPMet were increased by 3.9% (P>0.05), 10.2% (P>0.05) and 24.4% (P>0.05), respectively. Therefore, dietary RPLys and RPMet supplementation, to some extent, improve performance and milk quality of lactating water buffalo.［Chinese Journal of Animal Nutrition, 2010，22（5）:1445-1450］