本研究旨在探讨半纯化日粮中添加不同含量的维生素B1（thiamine）对凡纳滨对虾（Litopenaeus vannamei）生长性能和生理生化指标的影响，评价凡纳滨对虾对维生素B1的需要量。以酪蛋白、明胶、鱼粉作为蛋白源，配制维生素B1含量分别为0.83、19.70、37.40、50.80、65.30、94.20、163.00和323.00 mg/kg的8种半纯化日粮，选择初始体重（0.55±0.03）g的凡纳滨对虾虾苗960尾，随机分为8组，每组3个重复，每个重复40尾，分别连续投喂42 d。结果显示，半纯化日粮中添加维生素B1能显著提高凡纳滨对虾的特定生长率和饲料效率（P<0.05）。添加维生素B1 19.70～50.80 mg/kg能显著提高凡纳滨对虾的存活率（P<0.05）。添加维生素B1对凡纳滨对虾的体成分和肝胰脏中的淀粉酶活性无显著影响（P>0.05）。添加维生素B1 50.80 mg/kg日粮组的血清转酮醇酶活性（TKA）最高，显著高于未添加（0.83 mg/kg）组（P<0.05），与其他添加组间差异不显著（P>0.05）。在本试验条件下，以生长性能和血清TKA为评价指标，通过折线模型分析得到凡纳滨对虾维生素B1需要量分别为23.90和23.70 mg/kg日粮。

An experiment was conducted to determine the requirement of dietary vitamin B1 (thiamine, TN) for juvenile Litopenaeus vannamei. Semi-purified basal diets were formulated using vitamin-free casein, gelatin and fish meal as protein resources. Graded levels of TN were added to the basal diet which analytically contained 0.83, 19.70, 37.40, 50.80, 65.30, 94.20, 163.00 and 323.00 mg/kg diet, respectively. Each diet was fed into tanks of three replicates of shrimp (mean weight of (0.55±0.03) g) for 42 days. Results indicated that the specific growth rate (SGR) and feed efficiency of shrimp fed diets supplemented with thiamine were significantly higher than those of shrimp fed non-supplemented diet (0.83 mg/kg diet) (P<0.05). Shrimp fed 19.70～50.80 mg/kg diets had a significantly higher survival rate (P<0.05). There was no significant difference in body composition and hepatopancreatic amylase activity among the groups (P>0.05). The activity of serum transketolase (TKA) was the highest in shrimp fed 50.8 mg/kg diet, significantly higher than that of shrimp fed the non-supplemented diet. SGR and TKA in serum were found to be suitable indicators, and analysis of broken-line regression indicated that the dietary vitamin B1 requirement of Litopenaeus vannamei was estimated to be 23.90 and 23.70 mg/kg diet, respectively.［Chinese Journal of Animal Nutrition, 2010，22（4）:977-984］