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动物营养学报 2019, Vol. 31 Issue (3) :1288-1294    DOI: 10.3969/j.issn.1006-267x.2019.03.034
饲料科学与技术 Feed science and technology 最新目录 | 下期目录 | 过刊浏览 | 高级检索 << Previous Articles | Next Articles >>
饲粮添加果胶寡糖对轮状病毒攻毒断奶仔猪回肠屏障功能的影响
毛湘冰, 陈浩, 陈代文, 余冰, 何军, 郑萍, 虞洁, 罗钧秋, 罗玉衡
四川农业大学动物营养研究所, 动物抗病营养教育部重点实验室, 成都 611130
Effects of Dietary Pectic Oligosaccharide on Ileal Barrier Function of Weaned Piglets Challenged by Rotavirus
MAO Xiangbing, CHEN Hao, CHEN Daiwen, YU Bing, HE Jun, ZHENG Ping, YU Jie, LUO Junqiu, LUO Yuheng
Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China
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摘要 本研究旨在探讨饲粮添加果胶寡糖对轮状病毒攻毒断奶仔猪回肠屏障功能的影响。试验选取28头健康的21日龄断奶的"杜×长×大"仔猪,采用2×2因子试验设计,即果胶寡糖处理(饲粮添加200 mg/kg果胶寡糖或不添加)和轮状病毒攻毒(灌服轮状病毒或无菌培养液),轮状病毒攻毒于试验第15天早上进行。试验期18 d。结果表明:轮状病毒攻毒提高了断奶仔猪的腹泻率和回肠黏膜中轮状病毒非结构蛋白4(NSP4)的阳性率,显著降低了回肠黏膜的绒毛高度和绒毛高度/隐窝深度值(P<0.05),显著降低了回肠黏膜中闭锁小带蛋白-1(ZO-1)、闭合蛋白(occludin)、黏蛋白(mucin)1和mucin2的表达(P<0.05),显著提高了回肠黏膜中丙二醛的含量和回肠食糜中大肠杆菌的数量(P<0.05),并显著降低了回肠黏膜中总抗氧化能力和回肠食糜中乳酸杆菌和总菌的数量(P<0.05);而饲粮添加果胶寡糖降低了轮状病毒攻毒断奶仔猪的腹泻率和回肠黏膜中轮状病毒NSP4的阳性率,并显著缓解了轮状病毒对断奶仔猪回肠形态结构、回肠黏膜中occludin、mucin1和mucin2的表达和抗氧化能力以及回肠食糜中微生物数量的影响(P<0.05)。综上所述,在断奶仔猪饲粮中添加果胶寡糖可以预防轮状病毒诱导发生的腹泻,而这一作用与其可改善回肠屏障功能有关。
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关键词果胶寡糖   断奶仔猪   轮状病毒   回肠屏障功能     
Abstract: This experiment was conducted to determine the effects of dietary pectic oligosaccharide (POS) on the ileal barrier function of weaned pigs challenged by rotavirus (RV). Twenty-eight healthy crossbred (Duroc×Landrace×Yorkshire) piglets weaned at 21 days of age were used in a 2×2 factorial design. The trial factors included POS treatment (control diet or supplementing 200 mg/kg POS diet) and RV challenge (oral gavage with RV or sterilized medium). On day 15, RV challenge was carried out. The trial duration was 18 days. The results showed that RV infection increased the diarrhea rate and positive rate of rotavirus non-structural protein 4 (NSP4) in ileal mucosa, significantly decreased the villus height and villus height to crypt depth ratio of ileal mucosa (P<0.05), significantly reduced the expressions of zonula occludens-1 (ZO-1), occludin, mucin 1 and mucin 2 in ileal mucosa (P<0.05), significantly enhanced the malondialdehyde content in ileal mucosa and E. coli population in ileal digesta (P<0.05), and significantly decreased total antioxidant capacity in ileal mucosa and the population of Lactobacillus and total bacteria in ileal digesta of weaned pigs (P<0.05). Dietary POS reduced the diarrhea rate and positive rate of rotavirus NSP4 in ileal mucosa of the RV-infected weaned piglets, and significantly alleviated the effects of RV challenge on ileal mucosa morphology, microbial population in ileal digesta, antioxidant capacity and expressions of occludin, mucin 1 and mucin 2 in ileal mucosa of weaned piglets (P<0.05). These results suggest that dietary POS can prevent the RV-induced diarrhea, which is associated with the improvement of ileal barrier function of weaned pigs.
Keywordspectic oligosaccharide,   weaned piglets,   rotavirus,   ileal barrier function     
收稿日期: 2018-08-14;
基金资助:

现代农业产业技术体系(CARS-35);四川省科技支撑项目(2016NYZ0052,2016NZ0006)

作者简介: 毛湘冰(1980-),男,湖南长沙人,副教授,博士,研究方向为动物营养与饲料科学。E-mail:acatmxb2003@163.com
引用本文:   
. 饲粮添加果胶寡糖对轮状病毒攻毒断奶仔猪回肠屏障功能的影响[J]. 动物营养学报, 2019,V31(3): 1288-1294
. Effects of Dietary Pectic Oligosaccharide on Ileal Barrier Function of Weaned Piglets Challenged by Rotavirus[J]. Chinese Journal of Animal Nutrition, 2019,V31(3): 1288-1294.
链接本文:  
http://www.chinajan.com/CN/10.3969/j.issn.1006-267x.2019.03.034     或     http://www.chinajan.com/CN/Y2019/V31/I3/1288
 
[1] 毛湘冰,冯小花,陈代文,等.饲粮添加苹果果胶寡糖对断奶大鼠生长性能、抗氧化能力和肠道健康的影响[J].动物营养学报,2016,28(9):2872-2877.
[2] 任文华,崔志洪.猪轮状病毒概述[J].畜牧兽医杂志,2005,24(5):21-25.
[3] DONELLI G,SUPERTI F.The rotavirus genus[J].Comparative Immunology,Microbiology and Infectious Diseases,1994,17(3/4):305-320.
[4] BARNETT B.Viral gastroenteritis[J].Medical Clinics of North America,1983,67(5):1031-1058.
[5] WU S P,YUAN L J,ZHANG Y G,et al.Probiotic Lactobacillus rhamnosus GG mono-association suppresses human rotavirus-induced autophagy in the gnotobiotic piglet intestine[J].Gut Pathogens,2013,5(1):22.
[6] MAO X B,XIAO X J,CHEN D W,et al.Dietary apple pectic oligosaccharide improves gut barrier function of rotavirus-challenged weaned pigs by increasing antioxidant capacity of enterocytes[J].Oncotarget,2017,8(54):92420-92430.
[7] MAO X B,GU C S,REN M,et al.L-isoleucine administration alleviates rotavirus infection and immune response in the weaned piglet model[J].Frontiers in Immunology,2018,9:1654.
[8] MAO X B,LIU M H,TANG J,et al.Dietary leucine supplementation improves the mucin production in the jejunal mucosa of the weaned pigs challenged by porcine rotavirus[J].PLoS One,2015,10(9):e0137380.
[9] MAO X B,GU C S,HU H Y,et al.Dietary Lactobacillus rhamnosus GG supplementation improves the mucosal barrier function in the intestine of weaned piglets challenged by porcine rotavirus[J].PLoS One,2016,11(1):e0146312.
[10] JUNTUNEN M,KIRJAVAINEN P V,OUWEHAND A C,et al.Adherence of probiotic bacteria to human intestinal mucus in healthy infants and during rotavirus infection[J].Clinical and Diagnostic Laboratory Immunology,2001,8(2):293-296.
[11] TIAN G,LIANG X F,CHEN D W,et al.Vitamin D3 supplementation alleviates rotavirus infection in pigs and IPEC-J2 cells via regulating the autophagy signaling pathway[J].The Journal of Steroid Biochemistry and Molecular Biology,2016,163:157-163.
[12] MAO X B,ZENG X F,QIAO S Y,et al.Specific roles of threonine in intestinal mucosal integrity and barrier function[J].Frontiers in Bioscience,2011,3:1192-1200.
[13] DEPLANCKE B,GASKINS H R.Microbial modulation of innate defense:goblet cells and the intestinal mucus layer[J].The American Journal of Clinical Nutrition,2001,73(6):1131S-1141S.
[14] KUREK-GÍRECKA A,RZEPECKA-STOJKO A,GÍRECKI M,et al.Structure and antioxidant activity of polyphenols derived from propolis[J].Molecules,2014,19(1):78-101.
[15] ELLIS R J,BRUCE K D,JENKINS C,et al.Comparison of the distal gut microbiota from people and animals in Africa[J].PLoS One,2013,8(1):e54783.
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